The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p.

Abstract:

:Genetic analysis in Arabidopsis has led to the identification of several genes that are required for auxin response. One of these genes, AXR1, encodes a protein related to yeast Aos1p, a protein that functions to activate the ubiquitin-related protein Smt3p. Here we report the identification of a new gene called TRANSPORT INHIBITOR RESPONSE 1 (TIR1). The tir1 mutants are deficient in a variety of auxin-regulated growth processes including hypocotyl elongation and lateral root formation. These results indicate that TIR1 is also required for normal response to auxin. Further, mutations in TIR1 display a synergistic interaction with mutations in AXR1, suggesting that the two genes function in overlapping pathways. The TIR1 protein contains a series of leucine-rich repeats and a recently identified motif called an F box. Sequence comparisons indicate that TIR1 is related to the yeast protein Grr1p and the human protein SKP2. Because Grr1p and other F-box proteins have been implicated in ubiquitin-mediated processes, we speculate that auxin response depends on the modification of a key regulatory protein(s) by ubiquitin or a ubiquitin-related protein.

journal_name

Genes Dev

journal_title

Genes & development

authors

Ruegger M,Dewey E,Gray WM,Hobbie L,Turner J,Estelle M

doi

10.1101/gad.12.2.198

subject

Has Abstract

pub_date

1998-01-15 00:00:00

pages

198-207

issue

2

eissn

0890-9369

issn

1549-5477

journal_volume

12

pub_type

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