Abstract:
:Genetic analysis in Arabidopsis has led to the identification of several genes that are required for auxin response. One of these genes, AXR1, encodes a protein related to yeast Aos1p, a protein that functions to activate the ubiquitin-related protein Smt3p. Here we report the identification of a new gene called TRANSPORT INHIBITOR RESPONSE 1 (TIR1). The tir1 mutants are deficient in a variety of auxin-regulated growth processes including hypocotyl elongation and lateral root formation. These results indicate that TIR1 is also required for normal response to auxin. Further, mutations in TIR1 display a synergistic interaction with mutations in AXR1, suggesting that the two genes function in overlapping pathways. The TIR1 protein contains a series of leucine-rich repeats and a recently identified motif called an F box. Sequence comparisons indicate that TIR1 is related to the yeast protein Grr1p and the human protein SKP2. Because Grr1p and other F-box proteins have been implicated in ubiquitin-mediated processes, we speculate that auxin response depends on the modification of a key regulatory protein(s) by ubiquitin or a ubiquitin-related protein.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Ruegger M,Dewey E,Gray WM,Hobbie L,Turner J,Estelle Mdoi
10.1101/gad.12.2.198subject
Has Abstractpub_date
1998-01-15 00:00:00pages
198-207issue
2eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract::The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title:Genes & development
pub_type: 杂志文章
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abstract::Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elonga...
journal_title:Genes & development
pub_type: 杂志文章,评审
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abstract::We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC mi...
journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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abstract::Specific recognition of splice sites within metazoan mRNA precursors (pre-mRNAs) is a potential stage for gene regulation by alternative splicing. Splicing factors of the SR protein family play a major role in this regulation, as they are required for early recognition of splice sites during spliceosome assembly. Here...
journal_title:Genes & development
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abstract::The Wnt pathway controls numerous developmental processes via the beta-catenin-TCF/LEF transcription complex. Deregulation of the pathway results in the aberrant accumulation of beta-catenin in the nucleus, often leading to cancer. Normally, cytoplasmic beta-catenin associates with APC and axin and is continuously pho...
journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2007-03-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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pub_type: 杂志文章
doi:10.1101/gad.2.12b.1745
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journal_title:Genes & development
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journal_title:Genes & development
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doi:10.1101/gad.179663.111
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abstract::Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title:Genes & development
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abstract::U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dom...
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pub_type: 杂志文章
doi:10.1101/gad.5.5.773
更新日期:1991-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.872801
更新日期:2001-03-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.846800
更新日期:2000-12-01 00:00:00
abstract::An RNA-binding protein gene (rbp1) from Drosophila melanogaster, encoding an RNA recognition motif and an Arg-Ser rich (RS) domain, has been characterized. The predicted amino acid sequence of rbp1 is similar to those of the human splicing factor ASF/SF2, the Drosophila nuclear phosphoprotein SRp55, and the Drosophila...
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pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Genes & development
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更新日期:2014-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.1.29
更新日期:1992-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-04-15 00:00:00
abstract::Our laboratory has been utilizing the Escherichia coli outer membrane protein LamB to study the mechanism of protein localization. Various lines of evidence suggest that, in addition to a signal sequence, regions within the mature protein are required for efficient localization. In particular, studies using LamB-LacZ ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.2.185
更新日期:1987-04-01 00:00:00
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journal_title:Genes & development
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更新日期:2003-03-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2002-03-15 00:00:00
abstract::Extensive DNA elimination via a DNA breakage and rejoining process occurs during macronuclear development in the hypotrich ciliate Euplotes crassus. The excision process involves the removal of short, unique segments of DNA (internal eliminated sequences; IESs) and at least two highly repetitive families of transposon...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.1.84
更新日期:1993-01-01 00:00:00