The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing.


:Piwi-piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions of most of the factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show that MOV10L1 exhibits 5'-to-3' directional RNA-unwinding activity in vitro and that a point mutation that abolishes this activity causes a failure in primary piRNA biogenesis in vivo. We demonstrate that MOV10L1 selectively binds piRNA precursor transcripts and is essential for the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Multiple analyses suggest an intimate coupling of piRNA precursor processing with elements of local secondary structures such as G quadruplexes. Our results support a model in which MOV10L1 RNA helicase activity promotes unwinding and funneling of the single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing.


Genes Dev


Genes & development


Vourekas A,Zheng K,Fu Q,Maragkakis M,Alexiou P,Ma J,Pillai RS,Mourelatos Z,Wang PJ




Has Abstract


2015-03-15 00:00:00














  • Multipotent hematopoietic cells susceptible to alternative double-strand break repair pathways that promote genome rearrangements.

    abstract::Chromosomal translocations are a hallmark of hematopoietic malignancies. The initial molecular events or pathways that lead to translocations in hematopoietic cells are largely unknown, particularly in the stem cell-enriched population postulated to be the initial target for these events. We used in vitro differentiat...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Francis R,Richardson C

    更新日期:2007-05-01 00:00:00

  • CREB mediates brain serotonin regulation of bone mass through its expression in ventromedial hypothalamic neurons.

    abstract::Serotonin is a bioamine regulating bone mass accrual differently depending on its site of synthesis. It decreases accrual when synthesized in the gut, and increases it when synthesized in the brain. The signal transduction events elicited by gut-derived serotonin once it binds to the Htr1b receptor present on osteobla...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Oury F,Yadav VK,Wang Y,Zhou B,Liu XS,Guo XE,Tecott LH,Schutz G,Means AR,Karsenty G

    更新日期:2010-10-15 00:00:00

  • Functional analysis and in vivo footprinting implicate the erythroid transcription factor GATA-1 as a positive regulator of its own promoter.

    abstract::Transcription of erythroid-expressed genes and normal erythroid development in vivo are dependent on a regulatory protein (GATA-1) that recognizes a consensus GATA motif. GATA-1 expression is itself restricted to erythroid progenitors and to two related hematopoietic lineages, megakaryocytes and mast cells. During cel...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Tsai SF,Strauss E,Orkin SH

    更新日期:1991-06-01 00:00:00

  • A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting.

    abstract::To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hugo SE,Cruz-Garcia L,Karanth S,Anderson RM,Stainier DY,Schlegel A

    更新日期:2012-02-01 00:00:00

  • The cAMP receptor CAR4 regulates axial patterning and cellular differentiation during late development of Dictyostelium.

    abstract::Pseudoplasmodia of developing Dictyostelium are organized with anteroposterior polarity. We have isolated CAR4, the gene for a new cell-surface, G protein-linked cAMP receptor. CAR4 mRNA is initially expressed during tip elongation and continues to accumulate into culmination. CAR4 is maximally expressed in pseudoplas...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Louis JM,Ginsburg GT,Kimmel AR

    更新日期:1994-09-01 00:00:00

  • Vein expression is induced by the EGF receptor pathway to provide a positive feedback loop in patterning the Drosophila embryonic ventral ectoderm.

    abstract::The presence of a single EGF receptor in Drosophila is contrasted by multiple ligands activating it. This work explores the role of two ligands, Spitz and Vein, in the embryonic ventral ectoderm. Spitz is a potent ligand, whereas Vein is an intrinsically weak activating ligand. We show that secreted Spitz emanating fr...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Golembo M,Yarnitzky T,Volk T,Shilo BZ

    更新日期:1999-01-15 00:00:00

  • EGF domain swap converts a drosophila EGF receptor activator into an inhibitor.

    abstract::In Drosophila the function of the epidermal growth factor (EGF) receptor is modulated zygotically by three EGF-like proteins: Spitz (Spi), which is a potent activator; Vein (Vn), which is a moderate activator; and Argos (Aos), which is an inhibitor. Chimeric molecules were constructed in which the EGF domain of Vn was...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Schnepp B,Donaldson T,Grumbling G,Ostrowski S,Schweitzer R,Shilo BZ,Simcox A

    更新日期:1998-04-01 00:00:00

  • The Caenorhabditis elegans SOMI-1 zinc finger protein and SWI/SNF promote regulation of development by the mir-84 microRNA.

    abstract::Hundreds of microRNAs (miRNAs) have been discovered in metazoans and plants, and understanding of their biogenesis has advanced dramatically; however, relatively little is known about the cofactors necessary for miRNA regulation of target gene expression. In Caenorhabditis elegans, the conserved miRNA let-7 and its pa...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hayes GD,Riedel CG,Ruvkun G

    更新日期:2011-10-01 00:00:00

  • RNase E action at a distance: degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria.

    abstract::A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by us...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Morita T,Aiba H

    更新日期:2011-02-15 00:00:00

  • FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis, encodes a protein with a zinc finger and HMG-related domains.

    abstract::Distinctive from that of the animal system, the basic plan of the plant body is the continuous formation of a structural unit, composed of a stem with a meristem at the top and lateral organs continuously forming at the meristem. Therefore, mechanisms controlling the formation, maintenance, and development of a merist...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sawa S,Watanabe K,Goto K,Liu YG,Shibata D,Kanaya E,Morita EH,Okada K

    更新日期:1999-05-01 00:00:00

  • A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

    abstract::In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to ionizing radiation (IR). Along with a number of known DDR ge...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hurov KE,Cotta-Ramusino C,Elledge SJ

    更新日期:2010-09-01 00:00:00

  • The proteasome regulates the UV-induced activation of the AP-1-like transcription factor Gcn4.

    abstract::The proteasome is well known for its regulation of the cell cycle and degradation of mis-folded proteins, yet many of its functions are still unknown. We show that RPN11, a gene encoding a subunit of the regulatory cap of the proteasome, is required for UV-stimulated activation of Gcn4p target genes, but is dispensabl...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Stitzel ML,Durso R,Reese JC

    更新日期:2001-01-15 00:00:00

  • Mismatch repair-dependent processing of methylation damage gives rise to persistent single-stranded gaps in newly replicated DNA.

    abstract::O(6)-Methylguanine ((Me)G) is a highly cytotoxic DNA modification generated by S(N)1-type methylating agents. Despite numerous studies implicating DNA replication, mismatch repair (MMR), and homologous recombination (HR) in (Me)G toxicity, its mode of action has remained elusive. We studied the molecular transactions ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Mojas N,Lopes M,Jiricny J

    更新日期:2007-12-15 00:00:00

  • WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.

    abstract::Tuberous sclerosis (TSC) is an autosomal dominant disease characterized by hamartoma formation in various organs and is caused by mutations targeting either the TSC1 or TSC2 genes. TSC1 and TSC2 proteins form a functionally interdependent dimeric complex. Phosphorylation of either TSC subunit by different kinases regu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y

    更新日期:2008-04-01 00:00:00

  • Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I.

    abstract::We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strain BL21(DE3) and is not...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Morham SG,Shuman S

    更新日期:1990-04-01 00:00:00

  • Fragile X-related protein and VIG associate with the RNA interference machinery.

    abstract::RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Dro...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Caudy AA,Myers M,Hannon GJ,Hammond SM

    更新日期:2002-10-01 00:00:00

  • Uniform gene expression in embryos is achieved by temporal averaging of transcription noise.

    abstract::Transcription is often stochastic. This is seemingly incompatible with the importance of gene expression during development. Here we show that during zebrafish embryogenesis, transcription activation is stochastic due to (1) genes acquiring transcriptional competence at different times in different cells, (2) differen...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Stapel LC,Zechner C,Vastenhouw NL

    更新日期:2017-08-15 00:00:00

  • CXCR4 and Gab1 cooperate to control the development of migrating muscle progenitor cells.

    abstract::Long-range migrating progenitor cells generate hypaxial muscle, for instance the muscle of the limbs, hypoglossal cord, and diaphragm. We show here that migrating muscle progenitors express the chemokine receptor CXCR4. The corresponding ligand, SDF1, is expressed in limb and branchial arch mesenchyme; i.e., along the...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Vasyutina E,Stebler J,Brand-Saberi B,Schulz S,Raz E,Birchmeier C

    更新日期:2005-09-15 00:00:00

  • Mst1 shuts off cytosolic antiviral defense through IRF3 phosphorylation.

    abstract::Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional factor of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how the status of IRF3 activation is controlled rem...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Meng F,Zhou R,Wu S,Zhang Q,Jin Q,Zhou Y,Plouffe SW,Liu S,Song H,Xia Z,Zhao B,Ye S,Feng XH,Guan KL,Zou J,Xu P

    更新日期:2016-05-01 00:00:00

  • Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization.

    abstract::Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We identified Zeppo1 (zinc finger elbow-related proline domain protein 1) (FLJ14299/ZNF703) within this amplicon as a regulator of cell adhesion, migration, and prol...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Slorach EM,Chou J,Werb Z

    更新日期:2011-03-01 00:00:00

  • Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells.

    abstract::Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we dem...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Inoue A,Chen Z,Yin Q,Zhang Y

    更新日期:2018-12-01 00:00:00

  • Polycomb protein Ezh2 regulates pancreatic beta-cell Ink4a/Arf expression and regeneration in diabetes mellitus.

    abstract::Proliferation of pancreatic islet beta cells is an important mechanism for self-renewal and for adaptive islet expansion. Increased expression of the Ink4a/Arf locus, which encodes the cyclin-dependent kinase inhibitor p16(INK4a) and tumor suppressor p19(Arf), limits beta-cell regeneration in aging mice, but the basis...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Chen H,Gu X,Su IH,Bottino R,Contreras JL,Tarakhovsky A,Kim SK

    更新日期:2009-04-15 00:00:00

  • Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast.

    abstract::Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells, but the effectors mediating this control remain unknown. Through the use of the complete yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus C...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Brown JL,Jaquenoud M,Gulli MP,Chant J,Peter M

    更新日期:1997-11-15 00:00:00

  • Nitric oxide directly regulates gene expression during Drosophila development: need some gas to drive into metamorphosis?

    abstract::Nitric oxide (NO) is an important second messenger involved in numerous biological processes, but how it regulates gene expression is not well understood. In this issue of Genes & Development, Cáceres and colleagues (pp. 1476-1485) report a critical requirement of NO as a direct regulator of gene expression through it...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Yamanaka N,O'Connor MB

    更新日期:2011-07-15 00:00:00

  • PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes.

    abstract::PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Yan Z,Cui K,Murray DM,Ling C,Xue Y,Gerstein A,Parsons R,Zhao K,Wang W

    更新日期:2005-07-15 00:00:00

  • Topoisomerases and yeast rRNA transcription: negative supercoiling stimulates initiation and topoisomerase activity is required for elongation.

    abstract::Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Schultz MC,Brill SJ,Ju Q,Sternglanz R,Reeder RH

    更新日期:1992-07-01 00:00:00

  • Mechanism of corepressor binding and release from nuclear hormone receptors.

    abstract::The association of transcription corepressors SMRT and N-CoR with retinoid and thyroid receptors results in suppression of basal transcriptional activity. A key event in nuclear receptor signaling is the hormone-dependent release of corepressor and the recruitment of coactivator. Biochemical and structural studies hav...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nagy L,Kao HY,Love JD,Li C,Banayo E,Gooch JT,Krishna V,Chatterjee K,Evans RM,Schwabe JW

    更新日期:1999-12-15 00:00:00

  • Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes.

    abstract::The Komeda miniature rat Ishikawa (KMI) is a naturally occurring mutant caused by an autosomal recessive mutation mri, which exhibits longitudinal growth retardation. Here we identified the mri mutation as a deletion in the rat gene encoding cGMP-dependent protein kinase type II (cGKII). KMIs showed an expanded growth...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Chikuda H,Kugimiya F,Hoshi K,Ikeda T,Ogasawara T,Shimoaka T,Kawano H,Kamekura S,Tsuchida A,Yokoi N,Nakamura K,Komeda K,Chung UI,Kawaguchi H

    更新日期:2004-10-01 00:00:00

  • Regulating the regulator: an RNA decoy acts as an OFF switch for the regulation of an sRNA.

    abstract::Many bacterial small regulatory RNAs (sRNAs) pair with mRNA targets, stimulating or inhibiting mRNA stability and/or translation. Regulation of these sRNAs is usually due to tight transcriptional regulation of synthesis.In this issue of Genes & Development and a related paper in Molecular Microbiology, Figueroa-Bossi ...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Mandin P,Gottesman S

    更新日期:2009-09-01 00:00:00

  • Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis.

    abstract::Entry into sporulation by the Gram-positive bacterium Bacillus subtilis is governed by two transcription factors, Spo0A and sigma H, and involves a switch in the site of division from a medial to a polar location. We report that at the onset of sporulation, assembly of the cell division protein FtsZ shifts from midcel...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Levin PA,Losick R

    更新日期:1996-02-15 00:00:00