Myc-mediated apoptosis requires wild-type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21waf1/cip1.


:Deregulated expression of the c-myc proto-oncogene can lead to apoptosis under certain physiological conditions. By introducing a conditionally active Myc allele into primary embryo fibroblasts null for p53, and into fibroblasts without endogenous p53 expression but ectopically expressing a temperature-sensitive p53 allele, we show that expression of wild-type p53 is required for susceptibility to Myc-mediated apoptosis. Although ectopic expression of wild-type p53 blocked cells in the G1 phase of the cell cycle, G1 arrest by isoleucine starvation, in a manner independent of p53, did not confer susceptibility to apoptosis. Thus, growth arrest per se is not sufficient to induce Myc-mediated apoptosis; instead, a property intrinsic to p53 is specifically required. Moreover, apoptosis did not require induction of p53 target proteins, including the cyclin-dependent kinase inhibitor p21waf1/cip1. Therefore, the role of p53 in apoptosis may be distinct from its role in cell cycle arrest.


Genes Dev


Genes & development


Wagner AJ,Kokontis JM,Hay N




Has Abstract


1994-12-01 00:00:00












  • Purification of the human histone H4 gene-specific transcription factors H4TF-1 and H4TF-2.

    abstract::Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Dailey L,Roberts SB,Heintz N

    更新日期:1988-12-01 00:00:00

  • Cell surface proteins Nasrat and Polehole stabilize the Torso-like extracellular determinant in Drosophila oogenesis.

    abstract::Structural cell-surface and extracellular-matrix proteins modulate intercellular signaling events during development, but how this is achieved remains largely unknown. Here we identify a novel family of Drosophila proteins, Nasrat and Polehole, that coat the oocyte surface and play two roles: They mediate assembly of ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jiménez G,González-Reyes A,Casanova J

    更新日期:2002-04-15 00:00:00

  • The loss of circadian PAR bZip transcription factors results in epilepsy.

    abstract::DBP (albumin D-site-binding protein), HLF (hepatic leukemia factor), and TEF (thyrotroph embryonic factor) are the three members of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family. All three of these transcriptional regulatory proteins accumulate with robust circadian...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gachon F,Fonjallaz P,Damiola F,Gos P,Kodama T,Zakany J,Duboule D,Petit B,Tafti M,Schibler U

    更新日期:2004-06-15 00:00:00

  • The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression.

    abstract::Cell cycle progression is dependent on two major waves of gene expression. Early cell cycle gene expression occurs during G1/S to generate factors required for DNA replication, while late cell cycle gene expression begins during G2 to prepare for mitosis. Here we demonstrate that the MuvB complex-comprised of LIN9, LI...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sadasivam S,Duan S,DeCaprio JA

    更新日期:2012-03-01 00:00:00

  • (CA/GT)(n) microsatellites affect homologous recombination during yeast meiosis.

    abstract::One of the most common microsatellites in eukaryotes consists of tandem arrays of the dinucleotide GT. Although the study of the instability of such repetitive DNA has been extremely fruitful over the last decade, no biological function has been demonstrated for these sequences. We investigated the genetic behavior of...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gendrel CG,Boulet A,Dutreix M

    更新日期:2000-05-15 00:00:00

  • A bZIP protein, sisterless-a, collaborates with bHLH transcription factors early in Drosophila development to determine sex.

    abstract::Sexual identity in Drosophila is determined by zygotic X-chromosome dose. Two potent indicators of X-chromosome dose are sisterless-a (sis-a) and sisterless-b (sis-b). Genetic analysis has shown that a diplo-X dose of these genes activates their regulatory target, the feminizing switch gene Sex-lethal (Sxl), whereas a...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Erickson JW,Cline TW

    更新日期:1993-09-01 00:00:00

  • Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing.

    abstract::To investigate functional differences between RNA polymerases IIA and IIO (Pol IIA and Pol IIO), with hypo- and hyperphosphorylated carboxy-terminal repeat domains (CTDs), respectively, we have visualized the in vivo distributions of the differentially phosphorylated forms of Pol II on Drosophila polytene chromosomes....

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Weeks JR,Hardin SE,Shen J,Lee JM,Greenleaf AL

    更新日期:1993-12-01 00:00:00

  • Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein.

    abstract::How dividing mammalian cells overcome blocks to DNA replication by DNA damage, depleted nucleotide pools, or template-bound proteins is unclear. Here, we show that the response to blocked replication requires BRCA2, a suppressor of human breast cancer. By using two-dimensional gel electrophoresis, we demonstrate that ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lomonosov M,Anand S,Sangrithi M,Davies R,Venkitaraman AR

    更新日期:2003-12-15 00:00:00

  • Glucose regulates protein interactions within the yeast SNF1 protein kinase complex.

    abstract::The SNF1 protein kinase is broadly conserved in eukaryotes and has been implicated in responses to environmental and nutritional stress. In yeast, the SNF1 kinase has a central role in the response to glucose starvation. SNF1 is associated with its activating subunit, SNF4, and other proteins in complexes. Using the t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jiang R,Carlson M

    更新日期:1996-12-15 00:00:00

  • Dynamic changes in histone modifications precede de novo DNA methylation in oocytes.

    abstract::Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are implicated in a...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Stewart KR,Veselovska L,Kim J,Huang J,Saadeh H,Tomizawa S,Smallwood SA,Chen T,Kelsey G

    更新日期:2015-12-01 00:00:00

  • RPS25 is essential for translation initiation by the Dicistroviridae and hepatitis C viral IRESs.

    abstract::Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the 5' untranslated region. The cr...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Landry DM,Hertz MI,Thompson SR

    更新日期:2009-12-01 00:00:00

  • Surfing with the tunicates into the post-genome era.

    abstract::This year is the centenary of Edward G. Conklin's signal findings in embryology: the elucidation of complete cell lineages and the discovery of localized maternal determinants. Conklin used ascidian embryos to elucidate universal principles in embryology. A century later, ascidians, or sea squirts, have not only enter...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Satoh N,Levine M

    更新日期:2005-10-15 00:00:00

  • Keratinocyte growth factor is required for hair development but not for wound healing.

    abstract::Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), is synthesized by skin fibroblasts. However, its mitogenic activity is on skin keratinocytes, where it is the most potent growth factor identified thus far. To explore KGF's function in vivo, we used embryonic stem cell technology to ge...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Guo L,Degenstein L,Fuchs E

    更新日期:1996-01-15 00:00:00

  • I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.

    abstract::The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ruben SM,Klement JF,Coleman TA,Maher M,Chen CH,Rosen CA

    更新日期:1992-05-01 00:00:00

  • Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57-kD RNA-binding protein.

    abstract::Translation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal internal entry into the 5'-nontranslated region (5' NTR) rather than by ribosomal scanning. The internal ribosomal entry site (IRES) in the EMCV 5' NTR was determined by in vitro translation with RNAs that were generated by in vitro transcriptio...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jang SK,Wimmer E

    更新日期:1990-09-01 00:00:00

  • ZNF445 is a primary regulator of genomic imprinting.

    abstract::Genomic imprinting is an epigenetic process regulated by germline-derived DNA methylation, causing parental origin-specific monoallelic gene expression. Zinc finger protein 57 (ZFP57) is critical for maintenance of this epigenetic memory during post-fertilization reprogramming, yet incomplete penetrance of ZFP57 mutat...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Takahashi N,Coluccio A,Thorball CW,Planet E,Shi H,Offner S,Turelli P,Imbeault M,Ferguson-Smith AC,Trono D

    更新日期:2019-01-01 00:00:00

  • pX, the HBV-encoded coactivator, suppresses the phenotypes of TBP and TAFII250 mutants.

    abstract::Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Haviv I,Matza Y,Shaul Y

    更新日期:1998-04-15 00:00:00

  • Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.

    abstract::The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Cherry S,Doukas T,Armknecht S,Whelan S,Wang H,Sarnow P,Perrimon N

    更新日期:2005-02-15 00:00:00

  • P-element-mediated enhancer detection: a versatile method to study development in Drosophila.

    abstract::We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of s...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bellen HJ,O'Kane CJ,Wilson C,Grossniklaus U,Pearson RK,Gehring WJ

    更新日期:1989-09-01 00:00:00

  • Dynamic expression and essential functions of Hes7 in somite segmentation.

    abstract::The basic helix-loop-helix (bHLH) gene Hes7, a putative Notch effector, encodes a transcriptional repressor. Here, we found that Hes7 expression oscillates in 2-h cycles in the presomitic mesoderm (PSM). In Hes7-null mice, somites are not properly segmented and their anterior-posterior polarity is disrupted. As a resu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bessho Y,Sakata R,Komatsu S,Shiota K,Yamada S,Kageyama R

    更新日期:2001-10-15 00:00:00

  • Combinatorial control of Drosophila circular RNA expression by intronic repeats, hnRNPs, and SR proteins.

    abstract::Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms by which these circular RNAs are generated are largely unknown. Here, using extensive muta...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kramer MC,Liang D,Tatomer DC,Gold B,March ZM,Cherry S,Wilusz JE

    更新日期:2015-10-15 00:00:00

  • Chromatin signatures of cancer.

    abstract::Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Morgan MA,Shilatifard A

    更新日期:2015-02-01 00:00:00

  • A two-base change in a POU factor-binding site switches pituitary-specific to lymphoid-specific gene expression.

    abstract::The structurally related POU homeo domain proteins Pit-1 and Oct-2 activate pituitary- and lymphoid-specific transcription, respectively, by binding to similar AT-rich motifs in their target genes. In this study we identify bases critical for recognition and activation by Pit-1 and examine how small differences in Pit...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Elsholtz HP,Albert VR,Treacy MN,Rosenfeld MG

    更新日期:1990-01-01 00:00:00

  • Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore.

    abstract::Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Zheng LB,Donovan WP,Fitz-James PC,Losick R

    更新日期:1988-08-01 00:00:00

  • Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase.

    abstract::Ribonucleotide reductase activity is essential for progression through the cell cycle, catalyzing the rate-limiting step for the production of deoxyribonucleotides needed for DNA synthesis. The enzymatic activity of the enzyme fluctuates in the cell cycle with an activity maximum in S phase. We have identified and cha...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Elledge SJ,Davis RW

    更新日期:1990-05-01 00:00:00

  • Foxl1 is a mesenchymal Modifier of Min in carcinogenesis of stomach and colon.

    abstract::Constitutive activation of the Wnt/APC/beta-catenin pathway is a frequent initiating event in gastrointestinal carcinogenesis. Mutations in the Adenomatous Polyposis Coli (APC) gene up-regulate Wnt signaling by stabilizing beta-catenin and causing activation of targets important in proliferation control. Here we show ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Perreault N,Sackett SD,Katz JP,Furth EE,Kaestner KH

    更新日期:2005-02-01 00:00:00

  • Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi.

    abstract::Cosuppression is a silencing phenomenon triggered by the introduction of homologous DNA sequences into the genomes of organisms as diverse as plants, fungi, flies, and nematodes. Here we report sex-induced silencing (SIS), which is triggered by tandem integration of a transgene array in the human fungal pathogen Crypt...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wang X,Hsueh YP,Li W,Floyd A,Skalsky R,Heitman J

    更新日期:2010-11-15 00:00:00

  • Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells.

    abstract::Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we dem...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Inoue A,Chen Z,Yin Q,Zhang Y

    更新日期:2018-12-01 00:00:00

  • Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target.

    abstract::A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and chara...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Figueroa-Bossi N,Valentini M,Malleret L,Fiorini F,Bossi L

    更新日期:2009-09-01 00:00:00

  • Different upstream transcriptional activators have distinct coactivator requirements.

    abstract::Activated transcription by RNA polymerase II (Pol II) requires coactivators, one of which is the SRB/mediator. Whereas Srb4, an essential subunit of the SRB/mediator, is broadly required for Pol II transcription in yeast, we have shown that it is dispensable for the transcriptional activation of some genes. Here, we s...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lee DK,Kim S,Lis JT

    更新日期:1999-11-15 00:00:00