A novel class of small RNAs in mouse spermatogenic cells.


:Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class of small RNAs in the mouse male germline termed piwi-interacting RNAs (piRNAs). piRNAs are approximately 30 nt in length. They are expressed during spermatogenesis, mostly in spermatids. piRNAs are associated with MIWI, a spermatogenesis-specific PIWI subfamily member of the Argonaute protein family, and depend on MIWI for their biogenesis and/or stability. Furthermore, a subpopulation of piRNAs are associated with polysomes, suggesting their potential role in translational regulation.


Genes Dev


Genes & development


Grivna ST,Beyret E,Wang Z,Lin H




Has Abstract


2006-07-01 00:00:00














  • Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila.

    abstract::Gut homeostasis is controlled by both immune and developmental mechanisms, and its disruption can lead to inflammatory disorders or cancerous lesions of the intestine. While the impact of bacteria on the mucosal immune system is beginning to be precisely understood, little is known about the effects of bacteria on gut...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Buchon N,Broderick NA,Chakrabarti S,Lemaitre B

    更新日期:2009-10-01 00:00:00

  • Methylation of RUNX1 by PRMT1 abrogates SIN3A binding and potentiates its transcriptional activity.

    abstract::RUNX1/AML1 is required for the development of definitive hematopoiesis, and its activity is altered by mutations, deletions, and chromosome translocations in human acute leukemia. RUNX1 function can be regulated by post-translational modifications and protein-protein interactions. We show that RUNX1 is arginine-methyl...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Zhao X,Jankovic V,Gural A,Huang G,Pardanani A,Menendez S,Zhang J,Dunne R,Xiao A,Erdjument-Bromage H,Allis CD,Tempst P,Nimer SD

    更新日期:2008-03-01 00:00:00

  • Differential requirements for cell-specific elastase I enhancer domains in transfected cells and transgenic mice.

    abstract::The 134-bp enhancer region of the pancreatic elastase I gene is sufficient to direct pancreatic acinar cell-specific transcription in transgenic mice and in transfected cells in culture. Ten-base-pair scanner mutations in three separate enhancer domains that inactivate enhancer function in transfected pancreatic cells...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Swift GH,Kruse F,MacDonald RJ,Hammer RE

    更新日期:1989-05-01 00:00:00

  • The 65-kD subunit of NF-kappa B is a receptor for I kappa B and a modulator of DNA-binding specificity.

    abstract::A recent study has shown that the dimeric form of the 50-kD DNA-binding subunit of the NF-kappa B transcription factor is commonly associated with two molecules of a 65-kD protein (p65) and that p65 appears to be required for inactivation of the p50 dimer by the inhibitory subunit I kappa B. Here, we provide evidence ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Urban MB,Baeuerle PA

    更新日期:1990-11-01 00:00:00

  • Transcript levels and translational control of hsp70 synthesis in Xenopus oocytes.

    abstract::Until recently it was believed that Xenopus oocytes respond to heat shock by synthesizing the 70-kD heat shock protein hsp70 and that, uniquely amongst animal cell types, this response is mediated entirely at the translational level. This view has now been challenged and we present data that reevaluate the involvement...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Horrell A,Shuttleworth J,Colman A

    更新日期:1987-07-01 00:00:00

  • Fgf-10 is required for both limb and lung development and exhibits striking functional similarity to Drosophila branchless.

    abstract::Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Min H,Danilenko DM,Scully SA,Bolon B,Ring BD,Tarpley JE,DeRose M,Simonet WS

    更新日期:1998-10-15 00:00:00

  • Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B.

    abstract::Transformation of primary rodent cells by the adenovirus E1A and E1B oncogenes is a two-step process, where E1A-dependent induction of proliferation is coupled to E1B-dependent suppression of programmed cell death (apoptosis). The E1B gene encodes two distinct transforming proteins, the 19K and 55K proteins, both of w...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Debbas M,White E

    更新日期:1993-04-01 00:00:00

  • Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.

    abstract::Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger J

    更新日期:2002-11-01 00:00:00

  • The relative ratio of condensin I to II determines chromosome shapes.

    abstract::To understand how chromosome shapes are determined by actions of condensins and cohesin, we devised a series of protocols in which their levels are precisely changed in Xenopus egg extracts. When the relative ratio of condensin I to II is forced to be smaller, embryonic chromosomes become shorter and thicker, being re...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Shintomi K,Hirano T

    更新日期:2011-07-15 00:00:00

  • The HAT4 gene of Arabidopsis encodes a developmental regulator.

    abstract::The HAT4 gene from the plant Arabidopsis thaliana encodes a homeo domain protein that contains a leucine zipper motif. Homeo domain-leucine zipper (HD-Zip) proteins have not been found in animal systems, suggesting that HAT4 may define a new family of transcription factors that regulate higher plant development. To ex...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Schena M,Lloyd AM,Davis RW

    更新日期:1993-03-01 00:00:00

  • Core promoter elements can regulate transcription on a separate chromosome in trans.

    abstract::Transvection can cause the expression of a gene to be sensitive to the proximity of a homolog. It can account for many cases of intragenic complementation at the Drosophila yellow gene, where one mode of transvection involves the action of enhancers in trans on a promoter present on a separate chromosome. Our goal was...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Morris JR,Geyer PK,Wu CT

    更新日期:1999-02-01 00:00:00

  • USP21 deubiquitinase promotes pancreas cancer cell stemness via Wnt pathway activation.

    abstract::The ubiquitin-specific protease (USP) family is the largest group of cysteine proteases. Cancer genomic analysis identified frequent amplification of USP21 (22%) in human pancreatic ductal adenocarcinoma (PDAC). USP21 overexpression correlates with human PDAC progression, and enforced expression of USP21 accelerates m...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hou P,Ma X,Zhang Q,Wu CJ,Liao W,Li J,Wang H,Zhao J,Zhou X,Guan C,Ackroyd J,Jiang S,Zhang J,Spring DJ,Wang YA,DePinho RA

    更新日期:2019-10-01 00:00:00

  • A tetramer of the Flp recombinase silences the trimers within it during resolution of a Holliday junction substrate.

    abstract::Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining of four DNA strands between two target substrates. The reaction is carried out in two steps of pairwise strand exchanges by a DNA-protein assembly in which four Flp monomers act cooperatively to execute strand cleavage and joini...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lee J,Jayaram M

    更新日期:1997-09-15 00:00:00

  • Coregulation of anterior and posterior mesendodermal development by a hairy-related transcriptional repressor.

    abstract::During embryonic development in vertebrates, the endoderm becomes patterned along the anteroposterior axis to produce distinct derivatives. How this regulation is controlled is not well understood. We report that the zebrafish hairy/enhancer of split [E(spl)]-related gene her5 plays a critical role in this process. At...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bally-Cuif L,Goutel C,Wassef M,Wurst W,Rosa F

    更新日期:2000-07-01 00:00:00

  • Regulation of splicing at an intermediate step in the formation of the spliceosome.

    abstract::In vivo experiments have demonstrated that the ribosomal protein L32 of Saccharomyces cerevisiae brings about the inhibition of splicing of the transcript of its own gene through an RNA structure comprised largely of the first exon. We now show that L32, itself, binds specifically to this RNA. Splicing of the RPL32 tr...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Vilardell J,Warner JR

    更新日期:1994-01-01 00:00:00

  • Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

    abstract::The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRN...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bouvet P,Dimitrov S,Wolffe AP

    更新日期:1994-05-15 00:00:00

  • RNase E action at a distance: degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria.

    abstract::A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by us...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Morita T,Aiba H

    更新日期:2011-02-15 00:00:00

  • The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.

    abstract::Although the human U2 and U6 snRNA genes are transcribed by different RNA polymerases (i.e., RNA polymerases II and III, respectively), their promoters are very similar in structure. Both contain a proximal sequence element (PSE) and an octamer motif-containing enhancer, and these elements are interchangeable between ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lobo SM,Lister J,Sullivan ML,Hernandez N

    更新日期:1991-08-01 00:00:00

  • Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster.

    abstract::We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bishop JG 3rd,Corces VG

    更新日期:1988-05-01 00:00:00

  • Developmental control of the G1 to S transition in Drosophila: cyclin Eis a limiting downstream target of E2F.

    abstract::The E2F transcription factor is required for S phase in Drosophila. While it also triggers expression of replication genes at the G1-S transition, the relevance of this transcription is not clear because many of the induced gene products are sufficiently stable that new expression is not required for S phase. However,...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Duronio RJ,O'Farrell PH

    更新日期:1995-06-15 00:00:00

  • Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4.

    abstract::Histone deacetylase 3 (HDAC3) is one of four members of the human class I HDACs that regulates gene expression by deacetylation of histones and nonhistone proteins. Early studies have suggested that HDAC3 activity is regulated by association with the corepressors N-CoR and SMRT. Here we demonstrate that, in addition t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Zhang X,Ozawa Y,Lee H,Wen YD,Tan TH,Wadzinski BE,Seto E

    更新日期:2005-04-01 00:00:00

  • scratch, a pan-neural gene encoding a zinc finger protein related to snail, promotes neuronal development.

    abstract::The Drosophila scratch (scrt) gene is expressed in most or all neuronal precursor cells and encodes a predicted zinc finger transcription factor closely related to the product of the mesoderm determination gene snail (sna). Adult flies homozygous for scrt null alleles have a reduced number of photoreceptors in the eye...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Roark M,Sturtevant MA,Emery J,Vaessin H,Grell E,Bier E

    更新日期:1995-10-01 00:00:00

  • Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle.

    abstract::Disruptions in the use of skeletal muscle lead to muscle atrophy. After short periods of disuse, muscle atrophy is reversible, and even after prolonged periods of inactivity, myofiber degeneration is uncommon. The pathways that regulate atrophy, initiated either by peripheral nerve damage, immobilization, aging, catab...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wang X,Blagden C,Fan J,Nowak SJ,Taniuchi I,Littman DR,Burden SJ

    更新日期:2005-07-15 00:00:00

  • A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

    abstract::P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Karpen GH,Schaefer JE,Laird CD

    更新日期:1988-12-01 00:00:00

  • I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.

    abstract::The NF-kappa B transcription factor complex is comprised of two subunits, p50 and p65, that share significant homology to the rel oncogene. We have isolated a cDNA encoding a novel 66-kD rel-related protein, designated I-Rel. Unlike other rel-related proteins, I-Rel does not interact with DNA. I-Rel forms heterodimers...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ruben SM,Klement JF,Coleman TA,Maher M,Chen CH,Rosen CA

    更新日期:1992-05-01 00:00:00

  • Brachyury downstream notochord differentiation in the ascidian embryo.

    abstract::The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Takahashi H,Hotta K,Erives A,Di Gregorio A,Zeller RW,Levine M,Satoh N

    更新日期:1999-06-15 00:00:00

  • CDK12 phosphorylates 4E-BP1 to enable mTORC1-dependent translation and mitotic genome stability.

    abstract::The RNA polymerase II (RNAPII) C-terminal domain kinase, CDK12, regulates genome stability, expression of DNA repair genes, and cancer cell resistance to chemotherapy and immunotherapy. In addition to its role in mRNA biosynthesis of DNA repair genes, we show here that CDK12 phosphorylates the mRNA 5' cap-binding repr...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Choi SH,Martinez TF,Kim S,Donaldson C,Shokhirev MN,Saghatelian A,Jones KA

    更新日期:2019-04-01 00:00:00

  • The proteasome regulates the UV-induced activation of the AP-1-like transcription factor Gcn4.

    abstract::The proteasome is well known for its regulation of the cell cycle and degradation of mis-folded proteins, yet many of its functions are still unknown. We show that RPN11, a gene encoding a subunit of the regulatory cap of the proteasome, is required for UV-stimulated activation of Gcn4p target genes, but is dispensabl...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Stitzel ML,Durso R,Reese JC

    更新日期:2001-01-15 00:00:00

  • Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.

    abstract::To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty-seven percent of the introns assayed manifest >50% cotranscriptional splicing. The remaining 13% are cotranscriptionally spliced poorly or slow...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Khodor YL,Rodriguez J,Abruzzi KC,Tang CH,Marr MT 2nd,Rosbash M

    更新日期:2011-12-01 00:00:00

  • Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.

    abstract::In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Marini NJ,Reed SI

    更新日期:1992-04-01 00:00:00