The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.

abstract：:Growth of numerous cancer types is believed to be driven by a subpopulation of poorly differentiated cells, often referred to as cancer stem cells (CSCs), that have the capacity for self-renewal, tumor initiation, and generation of nontumorigenic progeny. Despite their potentially key role in tumor establishment and m...

journal_title：Genes & development

authors： Janiszewska M,Suvà ML,Riggi N,Houtkooper RH,Auwerx J,Clément-Schatlo V,Radovanovic I,Rheinbay E,Provero P,Stamenkovic I

更新日期：2012-09-01 00:00:00

abstract：:Spliceosome assembly has been characterized as the ordered association of the snRNP particles U1, U2, and U4/U6.U5 onto pre-mRNA. We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cerevisiae nuclear extracts to examine the first step of this process, 5' splice site recognition. This trans-s...

journal_title：Genes & development

authors： Johnson TL,Abelson J

更新日期：2001-08-01 00:00:00

abstract：:The gene encoding yeast U6 snRNA that is transcribed by RNA polymerase III (Pol III) contains both a TATA box upstream of the transcription start site and a downstream binding site for the factor TFIIIC. This juxtaposition of elements typical of both Pol II- and Pol III-transcribed genes raises the question of how pol...

journal_title：Genes & development

authors： Roberts S,Colbert T,Hahn S

更新日期：1995-04-01 00:00:00

abstract：:The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...

journal_title：Genes & development

authors： Bell-Pedersen D,Dunlap JC,Loros JJ

更新日期：1992-12-01 00:00:00

abstract：:Bone metastases are the most common skeletal complication of malignancy. Tumor cells disrupt normal bone remodeling to promote bone destruction and its associated morbidity. In the August 15, 2009, issue of Genes & Development, Lu and colleagues (pp. 1882-1894) propose a novel molecular mechanism by which tumor-produc...

journal_title：Genes & development

authors： Guise TA

更新日期：2009-09-15 00:00:00

abstract：:Chk2/hcds1, the human homolog of the Saccharomyces cerevisiae RAD53/SPK1 and Schizosaccharomyces pombe cds1 DNA damage checkpoint genes, encodes a protein kinase that is post-translationally modified after DNA damage. Like its yeast homologs, the Chk2/hCds1 protein phosphorylates Cdc25C in vitro, suggesting that it ar...

journal_title：Genes & development

authors： Chehab NH,Malikzay A,Appel M,Halazonetis TD

更新日期：2000-02-01 00:00:00

abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...

journal_title：Genes & development

authors： Waterman ML,Fischer WH,Jones KA

更新日期：1991-04-01 00:00:00

abstract：:Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...

journal_title：Genes & development

authors： Jiang YW,Stillman DJ

更新日期：1996-03-01 00:00:00

abstract：:Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...

journal_title：Genes & development

authors： Mueller JE,Smith D,Belfort M

更新日期：1996-09-01 00:00:00

abstract：:The timing of asexual fruiting body formation during Aspergillus nidulans colony development is precisely regulated so that conidiophores are typically produced 1-2 mm behind the growing edge of the colony. Mutations in any of four A. nidulans genes, flbB, flbC, flbD, or flbE, result in colonies that are delayed at le...

journal_title：Genes & development

authors： Wieser J,Adams TH

更新日期：1995-02-15 00:00:00

abstract：:Commitment to mitosis is regulated by a protein kinase complex called MPF. MPF is inhibited by Wee1-related kinases and activated by Cdc25 phosphatase. MPF activation further boosts Cdc25 and represses Wee1. This feedback control probably involves polo kinase. A dominant cut12.s11 mutation in the Schizosaccharomyces p...

journal_title：Genes & development

authors： MacIver FH,Tanaka K,Robertson AM,Hagan IM

更新日期：2003-06-15 00:00:00

abstract：:Structural cell-surface and extracellular-matrix proteins modulate intercellular signaling events during development, but how this is achieved remains largely unknown. Here we identify a novel family of Drosophila proteins, Nasrat and Polehole, that coat the oocyte surface and play two roles: They mediate assembly of ...

journal_title：Genes & development

authors： Jiménez G,González-Reyes A,Casanova J

更新日期：2002-04-15 00:00:00

abstract：:We describe three strains of transgenic mice derived by embryo microinjection of DNA consisting of a long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MSV) linked to the murine c-mos coding sequences. Southern analysis of the genomic DNA of these strains suggested that in each case the transgene had integ...

journal_title：Genes & development

authors： Khillan JS,Oskarsson MK,Propst F,Kuwabara T,Vande Woude GF,Westphal H

更新日期：1987-12-01 00:00:00

abstract：:Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein ac...

journal_title：Genes & development

authors： Yanagawa S,van Leeuwen F,Wodarz A,Klingensmith J,Nusse R

更新日期：1995-05-01 00:00:00

abstract：:Transcription factors represent one of the largest groups of proteins regulated by SUMO (small ubiquitin-like modifier) modification, and their sumoylation is usually associated with transcriptional repression. To investigate whether sumoylation plays a general role in regulating transcription in yeast, we determined ...

journal_title：Genes & development

authors： Rosonina E,Duncan SM,Manley JL

更新日期：2010-06-15 00:00:00

abstract：:The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, inclu...

journal_title：Genes & development

authors： Tanos BE,Yang HJ,Soni R,Wang WJ,Macaluso FP,Asara JM,Tsou MF

更新日期：2013-01-15 00:00:00

abstract：:In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...

journal_title：Genes & development

authors： Homer HA,McDougall A,Levasseur M,Yallop K,Murdoch AP,Herbert M

更新日期：2005-01-15 00:00:00

abstract：:Tissue morphogenesis during development is dependent on activities of the cadherin family of cell-cell adhesion proteins that includes classical cadherins, protocadherins, and atypical cadherins (Fat, Dachsous, and Flamingo). The extracellular domain of cadherins contains characteristic repeats that regulate homophili...

journal_title：Genes & development

authors： Halbleib JM,Nelson WJ

更新日期：2006-12-01 00:00:00

abstract：:Cohesin is a chromosome-associated multisubunit protein complex that is highly conserved in eukaryotes and has close homologs in bacteria. Cohesin mediates cohesion between replicated sister chromatids and is therefore essential for chromosome segregation in dividing cells. Cohesin is also required for efficient repai...

journal_title：Genes & development

authors： Peters JM,Tedeschi A,Schmitz J

更新日期：2008-11-15 00:00:00

abstract：:Oxidative tissues such as heart undergo a dramatic perinatal mitochondrial biogenesis to meet the high-energy demands after birth. PPARgamma coactivator-1 (PGC-1) alpha and beta have been implicated in the transcriptional control of cellular energy metabolism. Mice with combined deficiency of PGC-1alpha and PGC-1beta ...

journal_title：Genes & development

authors： Lai L,Leone TC,Zechner C,Schaeffer PJ,Kelly SM,Flanagan DP,Medeiros DM,Kovacs A,Kelly DP

更新日期：2008-07-15 00:00:00

abstract：:Cell cycle transitions are often accompanied by the degradation of regulatory molecules. Targeting proteins to the proteasome for degradation is accomplished by the covalent addition of ubiquitin chains. The specificity of this pathway is largely dictated by a set of enzymes called ubiquitin ligases (or E3s). The anap...

journal_title：Genes & development

authors： Thornton BR,Toczyski DP

更新日期：2006-11-15 00:00:00

abstract：:Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, re...

journal_title：Genes & development

authors： Zhu L,Wilken J,Phillips NB,Narendra U,Chan G,Stratton SM,Kent SB,Weiss MA

更新日期：2000-07-15 00:00:00

abstract：:Repression of a-cell specific gene expression in yeast alpha cells requires MAT alpha 2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To investigate the possibi...

journal_title：Genes & development

authors： Cooper JP,Roth SY,Simpson RT

更新日期：1994-06-15 00:00:00

abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...

journal_title：Genes & development

authors： Belsky JA,MacAlpine HK,Lubelsky Y,Hartemink AJ,MacAlpine DM

更新日期：2015-01-15 00:00:00

abstract：:The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidire...

journal_title：Genes & development

authors： Yoon JH,Prakash L,Prakash S

更新日期：2010-01-15 00:00:00

abstract：:Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...

journal_title：Genes & development

authors： Sandri-Goldin RM

更新日期：1998-03-15 00:00:00

abstract：:The choice of self-renewal versus differentiation is a fundamental issue in stem cell and cancer biology. Neural progenitors of the Drosophila post-embryonic brain, larval neuroblasts (NBs), divide asymmetrically in a stem cell-like fashion to generate a self-renewing NB and a Ganglion Mother Cell (GMC), which divides...

journal_title：Genes & development

authors： Wang H,Somers GW,Bashirullah A,Heberlein U,Yu F,Chia W

更新日期：2006-12-15 00:00:00

abstract：:The incretin hormone GLP1 promotes islet-cell survival via the second messenger cAMP. Here we show that mice deficient in the activity of CREB, caused by expression of a dominant-negative A-CREB transgene in pancreatic beta-cells, develop diabetes secondary to beta-cell apoptosis. Remarkably, A-CREB severely disrupted...

journal_title：Genes & development

authors： Jhala US,Canettieri G,Screaton RA,Kulkarni RN,Krajewski S,Reed J,Walker J,Lin X,White M,Montminy M

更新日期：2003-07-01 00:00:00

abstract：:We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of s...

journal_title：Genes & development

authors： Bellen HJ,O'Kane CJ,Wilson C,Grossniklaus U,Pearson RK,Gehring WJ

更新日期：1989-09-01 00:00:00

abstract：:The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...

journal_title：Genes & development

authors： Casanova J,Struhl G

更新日期：1989-12-01 00:00:00