The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

abstract：:The daughterless (da) gene is known to have separate maternal and zygotic functions: Maternally supplied daughterless activity is required for proper sex determination and dosage compensation in female embryos, whereas loss of zygotically supplied da+ activity causes embryonic lethality in both male and female embryos...

journal_title：Genes & development

authors： Caudy M,Grell EH,Dambly-Chaudière C,Ghysen A,Jan LY,Jan YN

更新日期：1988-07-01 00:00:00

abstract：:We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...

journal_title：Genes & development

authors： Bishop JG 3rd,Corces VG

更新日期：1988-05-01 00:00:00

abstract：:The formation of the apical ectodermal ridge (AER) is critical for the distal outgrowth and patterning of the vertebrate limb. Recent work in the chick has demonstrated that interplay between the Wnt and Fgf signaling pathways is essential in the limb mesenchyme and ectoderm in the establishment and perhaps the mainte...

journal_title：Genes & development

authors： Barrow JR,Thomas KR,Boussadia-Zahui O,Moore R,Kemler R,Capecchi MR,McMahon AP

更新日期：2003-02-01 00:00:00

abstract：:Neurofibromatosis type 1 (NF1) is a prevalent genetic disorder that affects growth properties of neural-crest-derived cell populations. In addition, approximately one-half of NF1 patients exhibit learning disabilities. To characterize NF1 function both in vitro and in vivo, we circumvent the embryonic lethality of NF1...

journal_title：Genes & development

authors： Zhu Y,Romero MI,Ghosh P,Ye Z,Charnay P,Rushing EJ,Marth JD,Parada LF

更新日期：2001-04-01 00:00:00

abstract：:The bcl-6 proto-oncogene encodes a POZ/zinc finger transcriptional repressor expressed in germinal center (GC) B and T cells and required for GC formation and antibody affinity maturation. Deregulation of bcl-6 expression by chromosomal rearrangements and point mutations of the bcl-6 promoter region are implicated in ...

journal_title：Genes & development

authors： Niu H,Ye BH,Dalla-Favera R

更新日期：1998-07-01 00:00:00

abstract：:Expression of HNF-4, a transcription factor in the steroid hormone receptor superfamily, is detected only in the visceral endoderm of mouse embryos during gastrulation and is expressed in certain embryonic tissues from 8.5 days of gestation. To examine the role of HNF-4 during embryonic development, we disrupted the g...

journal_title：Genes & development

authors： Chen WS,Manova K,Weinstein DC,Duncan SA,Plump AS,Prezioso VR,Bachvarova RF,Darnell JE Jr

更新日期：1994-10-15 00:00:00

abstract：:A recent study has shown that the dimeric form of the 50-kD DNA-binding subunit of the NF-kappa B transcription factor is commonly associated with two molecules of a 65-kD protein (p65) and that p65 appears to be required for inactivation of the p50 dimer by the inhibitory subunit I kappa B. Here, we provide evidence ...

journal_title：Genes & development

authors： Urban MB,Baeuerle PA

更新日期：1990-11-01 00:00:00

abstract：:Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...

journal_title：Genes & development

authors： Kessler JD,Hasegawa H,Brun SN,Emmenegger BA,Yang ZJ,Dutton JW,Wang F,Wechsler-Reya RJ

更新日期：2009-01-15 00:00:00

abstract：:Activating JAK2 point mutations are implicated in the pathogenesis of myeloid and lymphoid malignancies, including high-risk B-cell acute lymphoblastic leukemia (B-ALL). In preclinical studies, treatment of JAK2 mutant leukemias with type I JAK2 inhibitors (e.g., Food and Drug Administration [FDA]-approved ruxolitinib...

journal_title：Genes & development

authors： Kim SK,Knight DA,Jones LR,Vervoort S,Ng AP,Seymour JF,Bradner JE,Waibel M,Kats L,Johnstone RW

更新日期：2018-06-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) can trigger repressive chromatin, but how they recruit silencing factors remains unclear. In Schizosaccharomyces pombe, heterochromatin assembly on transcribed noncoding pericentromeric repeats requires both RNAi and RNAi-independent mechanisms. In Saccharomyces cerevisiae, which lacks a ...

journal_title：Genes & development

authors： Marina DB,Shankar S,Natarajan P,Finn KJ,Madhani HD

更新日期：2013-09-01 00:00:00

abstract：:Unrepaired DNA lesions in the template strand block the replication fork. In yeast, Mec1 protein kinase-mediated replication checkpoint prevents the breakdown of replication forks and maintains viability in DNA-damaged cells going through the S phase. By ensuring that the replisome does not dissociate from the fork st...

journal_title：Genes & development

authors： Pagès V,Santa Maria SR,Prakash L,Prakash S

更新日期：2009-06-15 00:00:00

abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...

journal_title：Genes & development

authors： Waterman ML,Fischer WH,Jones KA

更新日期：1991-04-01 00:00:00

abstract：:Stem cells do not all respond the same way, but the mechanisms underlying this heterogeneity are not well understood. Here, we found that expression of Hes1 and its downstream genes oscillate in mouse embryonic stem (ES) cells. Those expressing low and high levels of Hes1 tended to differentiate into neural and mesode...

journal_title：Genes & development

authors： Kobayashi T,Mizuno H,Imayoshi I,Furusawa C,Shirahige K,Kageyama R

更新日期：2009-08-15 00:00:00

abstract：:Mammalian telomeres are coated by the sequence-specific, DNA-binding protein, TRF1, a negative regulator of telomere length. Previous results showed that ADP-ribosylation of TRF1 by tankyrase 1 released TRF1 from telomeres and promoted telomere elongation. We now show that loss of TRF1 from telomeres results in ubiqui...

journal_title：Genes & development

authors： Chang W,Dynek JN,Smith S

更新日期：2003-06-01 00:00:00

abstract：:Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked kinase (ILK) is an FA component that interacts with the cytoplasmic domains of integrins, recruits adaptor proteins that lin...

journal_title：Genes & development

authors： Sakai T,Li S,Docheva D,Grashoff C,Sakai K,Kostka G,Braun A,Pfeifer A,Yurchenco PD,Fässler R

更新日期：2003-04-01 00:00:00

abstract：:PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity...

journal_title：Genes & development

authors： Yan Z,Cui K,Murray DM,Ling C,Xue Y,Gerstein A,Parsons R,Zhao K,Wang W

更新日期：2005-07-15 00:00:00

abstract：:Two retrovirus promoter trap vectors (U3His and U3Neo) have been used to disrupt genes expressed in totipotent murine embryonal stem (ES) cells. Selection in L-histidinol or G418 produced clones in which the coding sequences for histidinol-dehydrogenase or neomycin-phosphotransferase were fused to sequences in or near...

journal_title：Genes & development

authors： von Melchner H,DeGregori JV,Rayburn H,Reddy S,Friedel C,Ruley HE

更新日期：1992-06-01 00:00:00

abstract：:Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...

journal_title：Genes & development

authors： Mueller JE,Smith D,Belfort M

更新日期：1996-09-01 00:00:00

abstract：:Using an immunochemical approach, we have identified a unique antigen, PEP (protein on ecdysone puffs), which is associated in third-instar larvae with the active ecdysone-regulated loci on polytene chromosomes; PEP is not associated with most intermolt puffs and is found on some, but not all, heat shock-induced puffs...

journal_title：Genes & development

authors： Amero SA,Elgin SC,Beyer AL

更新日期：1991-02-01 00:00:00

abstract：:The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BR...

journal_title：Genes & development

authors： Evans R,Fairley JA,Roberts SG

更新日期：2001-11-15 00:00:00

abstract：:The superoxide radical (O(2)(-)) has long been considered a major cause of aging. O(2)(-) in cytosolic, extracellular, and mitochondrial pools is detoxified by dedicated superoxide dismutase (SOD) isoforms. We tested the impact of each SOD isoform in Caenorhabditis elegans by manipulating its five sod genes and saw no...

journal_title：Genes & development

authors： Doonan R,McElwee JJ,Matthijssens F,Walker GA,Houthoofd K,Back P,Matscheski A,Vanfleteren JR,Gems D

更新日期：2008-12-01 00:00:00

abstract：:Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary ...

journal_title：Genes & development

authors： Sawatsubashi S,Murata T,Lim J,Fujiki R,Ito S,Suzuki E,Tanabe M,Zhao Y,Kimura S,Fujiyama S,Ueda T,Umetsu D,Ito T,Takeyama K,Kato S

更新日期：2010-01-15 00:00:00

abstract：:In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...

journal_title：Genes & development

authors： Homer HA,McDougall A,Levasseur M,Yallop K,Murdoch AP,Herbert M

更新日期：2005-01-15 00:00:00

abstract：:TP53 is the most frequently mutated gene in human cancer. Many mutant p53 proteins exert oncogenic gain-of-function (GOF) properties that contribute to metastasis, but the mechanisms mediating these functions remain poorly defined in vivo. To elucidate how mutant p53 GOF drives metastasis, we developed a traceable som...

journal_title：Genes & development

authors： Pourebrahim R,Zhang Y,Liu B,Gao R,Xiong S,Lin PP,McArthur MJ,Ostrowski MC,Lozano G

更新日期：2017-09-15 00:00:00

abstract：:We describe here a new murine homeo box gene, denoted Hlx, which is expressed within specific hematopoietic lineages. The cDNA sequence indicates that Hlx differs markedly from known vertebrate homeo box genes, and linkage analysis of an interspecific murine backcross showed that it resides at a novel homeo box locus ...

journal_title：Genes & development

authors： Allen JD,Lints T,Jenkins NA,Copeland NG,Strasser A,Harvey RP,Adams JM

更新日期：1991-04-01 00:00:00

abstract：:Although MAX is regarded as an obligate dimerization partner for MYC, its function in normal development and neoplasia is poorly defined. We show that B-cell-specific deletion of Max has a modest effect on B-cell development but completely abrogates Eµ-Myc-driven lymphomagenesis. While Max loss affects only a few hund...

journal_title：Genes & development

authors： Mathsyaraja H,Freie B,Cheng PF,Babaeva E,Catchpole JT,Janssens D,Henikoff S,Eisenman RN

更新日期：2019-09-01 00:00:00

abstract：:Mammalian cells possess two amino acid-sensing kinases: general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). Their combined effects orchestrate cellular adaptation to amino acid levels, but how their activities are coordinated remains poorly understood. Here, we demonstrate...

journal_title：Genes & development

authors： Ye J,Palm W,Peng M,King B,Lindsten T,Li MO,Koumenis C,Thompson CB

更新日期：2015-11-15 00:00:00

abstract：:Mammalian histone gene transcription is increased approximately fivefold during the transition from the G1 phase to the S phase of the cell cycle. In this study, we present a detailed in vivo analysis of the human histone H2b promoter, which establishes that transcriptional regulation of this gene is mediated by a sub...

journal_title：Genes & development

authors： LaBella F,Sive HL,Roeder RG,Heintz N

更新日期：1988-01-01 00:00:00

abstract：:The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C ...

journal_title：Genes & development

authors： Cherry S,Doukas T,Armknecht S,Whelan S,Wang H,Sarnow P,Perrimon N

更新日期：2005-02-15 00:00:00

abstract：:Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms by which these circular RNAs are generated are largely unknown. Here, using extensive muta...

journal_title：Genes & development

authors： Kramer MC,Liang D,Tatomer DC,Gold B,March ZM,Cherry S,Wilusz JE

更新日期：2015-10-15 00:00:00