Abstract:
:Stem cells do not all respond the same way, but the mechanisms underlying this heterogeneity are not well understood. Here, we found that expression of Hes1 and its downstream genes oscillate in mouse embryonic stem (ES) cells. Those expressing low and high levels of Hes1 tended to differentiate into neural and mesodermal cells, respectively. Furthermore, inactivation of Hes1 facilitated neural differentiation more uniformly at earlier time. Thus, Hes1-null ES cells display less heterogeneity in both the differentiation timing and fate choice, suggesting that the cyclic gene Hes1 contributes to heterogeneous responses of ES cells even under the same environmental conditions.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Kobayashi T,Mizuno H,Imayoshi I,Furusawa C,Shirahige K,Kageyama Rdoi
10.1101/gad.1823109subject
Has Abstractpub_date
2009-08-15 00:00:00pages
1870-5issue
16eissn
0890-9369issn
1549-5477pii
23/16/1870journal_volume
23pub_type
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