Abstract:
:Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis of transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 transcriptional regulators and identified 78 genes involved in patterning the stereotyped dendritic arbors of class I da neurons in Drosophila. Most of these transcriptional regulators affect dendrite morphology without altering the number of class I dendrite arborization (da) neurons and fall primarily into three groups. Group A genes control both primary dendrite extension and lateral branching, hence the overall dendritic field. Nineteen genes within group A act to increase arborization, whereas 20 other genes restrict dendritic coverage. Group B genes appear to balance dendritic outgrowth and branching. Nineteen group B genes function to promote branching rather than outgrowth, and two others have the opposite effects. Finally, 10 group C genes are critical for the routing of the dendritic arbors of individual class I da neurons. Thus, multiple genetic programs operate to calibrate dendritic coverage, to coordinate the elaboration of primary versus secondary branches, and to lay out these dendritic branches in the proper orientation.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Parrish JZ,Kim MD,Jan LY,Jan YNdoi
10.1101/gad.1391006subject
Has Abstractpub_date
2006-04-01 00:00:00pages
820-35issue
7eissn
0890-9369issn
1549-5477pii
gad.1391006journal_volume
20pub_type
杂志文章abstract::The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.14.1812
更新日期:1996-07-15 00:00:00
abstract::Organization into polarized three-dimensional structures defines whether epithelial cells are normal or malignant. In a model of morphogenesis, we show that inhibiting key signaling pathways in human breast cancer cells leads to "phenotypic reversion" of the malignant cells. Using architecture as an endpoint, we repor...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1990410
更新日期:2010-12-15 00:00:00
abstract::The growth suppression function of the retinoblastoma protein (RB) is mediated by its interaction with a variety of cellular proteins. RB contains at least two protein-binding pockets: the large A/B pocket, which interacts with E2F and the D-type cyclins, and the C pocket, which interacts with the nuclear c-Abl tyrosi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.1.31
更新日期:1995-01-01 00:00:00
abstract::In meiosis I, homologous chromosomes pair and then attach to the spindle so that the homologs can be pulled apart at anaphase I. The segregation of homologs before pairing would be catastrophic. We describe two mechanisms that prevent this. First, in early meiosis, Ipl1, the budding yeast homolog of the mammalian Auro...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.227454.113
更新日期:2013-10-01 00:00:00
abstract::We characterized a gene, extradenticle, which seems to interact with a specific subset of Drosophila homeo domain proteins, possibly affecting their target specificity. This interpretation is based on an examination of the zygotic and maternal effect phenotypes of extradenticle mutations. In embryos with reduced level...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.7.1209
更新日期:1990-07-01 00:00:00
abstract::Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2'-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.268482.115
更新日期:2015-09-15 00:00:00
abstract::To investigate functional differences between RNA polymerases IIA and IIO (Pol IIA and Pol IIO), with hypo- and hyperphosphorylated carboxy-terminal repeat domains (CTDs), respectively, we have visualized the in vivo distributions of the differentially phosphorylated forms of Pol II on Drosophila polytene chromosomes....
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.12a.2329
更新日期:1993-12-01 00:00:00
abstract::Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1265905
更新日期:2005-01-15 00:00:00
abstract::Changes in the pattern of gene expression play an important role in allowing cancer cells to acquire their hallmark characteristics, while genomic instability enables cells to acquire genetic alterations that promote oncogenesis. Chromatin plays central roles in both transcriptional regulation and the maintenance of g...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.255182.114
更新日期:2015-02-01 00:00:00
abstract::A conditional block to transcriptional elongation is an important mechanism for regulating c-myc gene expression. This elongation block within the first c-myc exon was defined originally in mammalian cells by nuclear run-on transcription analyses. Subsequent oocyte injection and in vitro transcription analyses suggest...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.11.2201
更新日期:1992-11-01 00:00:00
abstract::RBP is a cellular protein that functions as a transcriptional repressor in mammalian cells. RBP has elicited great interest lately because of its established roles in regulating gene expression, in Drosophila and mouse development, and as a component of the Notch signal transduction pathway. This report focuses on the...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.11.1621
更新日期:1998-06-01 00:00:00
abstract::The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.187328.112
更新日期:2012-04-01 00:00:00
abstract::The balance between proliferation and differentiation of muscle stem cells is tightly controlled, ensuring the maintenance of a cellular pool needed for muscle growth and repair. We demonstrate here that the transcriptional regulator Hes1 controls the balance between proliferation and differentiation of activated musc...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.322818.118
更新日期:2019-05-01 00:00:00
abstract::Overexpression of the type II transmembrane serine protease matriptase is a highly consistent feature of human epithelial tumors. Here we show that matriptase possesses a strong oncogenic potential when unopposed by its endogenous inhibitor, HAI-1. Modest orthotopic overexpression of matriptase in the skin of transgen...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1300705
更新日期:2005-08-15 00:00:00
abstract::Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accu...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.287404
更新日期:2004-06-15 00:00:00
abstract::Stem cells do not all respond the same way, but the mechanisms underlying this heterogeneity are not well understood. Here, we found that expression of Hes1 and its downstream genes oscillate in mouse embryonic stem (ES) cells. Those expressing low and high levels of Hes1 tended to differentiate into neural and mesode...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1823109
更新日期:2009-08-15 00:00:00
abstract::We report on the function of the human ortholog of Saccharomyces cerevisiae Rif1 (Rap1-interacting factor 1). Yeast Rif1 associates with telomeres and regulates their length. In contrast, human Rif1 did not accumulate at functional telomeres, but localized to dysfunctional telomeres and to telomeric DNA clusters in AL...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1216004
更新日期:2004-09-01 00:00:00
abstract::Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.17.2158
更新日期:1996-09-01 00:00:00
abstract::We have developed a new technique, called 'toeprinting,' which has allowed a study of the tRNA-binding properties of Escherichia coli translation initiation complexes. In response to natural mRNAs, the initiator tRNA and a variety of elongator tRNAs bind to the same tRNA-binding site on the 30S ribosomal subunit as lo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.12a.1899
更新日期:1989-12-01 00:00:00
abstract::Transcriptional profiling is a powerful approach for understanding development and disease. Current cell type-specific RNA purification methods have limitations, including cell dissociation trauma or inability to identify all RNA species. Here, we describe "mouse thiouracil (TU) tagging," a genetic and chemical inters...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.205278.112
更新日期:2013-01-01 00:00:00
abstract::In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1511907
更新日期:2007-02-15 00:00:00
abstract::Our laboratory has been utilizing the Escherichia coli outer membrane protein LamB to study the mechanism of protein localization. Various lines of evidence suggest that, in addition to a signal sequence, regions within the mature protein are required for efficient localization. In particular, studies using LamB-LacZ ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.2.185
更新日期:1987-04-01 00:00:00
abstract::Pre-rRNA transcription by RNA Polymerase I (Pol I) is very robust on active rDNA repeats. Loss of yeast Topoisomerase I (Top1) generated truncated pre-rRNA fragments, which were stabilized in strains lacking TRAMP (Trf4/Trf5-Air1/Air2-Mtr4 polyadenylation complexes) or exosome degradation activities. Loss of both Top1...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.573310
更新日期:2010-07-15 00:00:00
abstract::O(6)-Methylguanine ((Me)G) is a highly cytotoxic DNA modification generated by S(N)1-type methylating agents. Despite numerous studies implicating DNA replication, mismatch repair (MMR), and homologous recombination (HR) in (Me)G toxicity, its mode of action has remained elusive. We studied the molecular transactions ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.455407
更新日期:2007-12-15 00:00:00
abstract::Known prions (infectious proteins) are self-propagating amyloids or conformationally altered proteins, but in theory an enzyme necessary for its own activation could also be a prion (or a gene composed of protein). We show that yeast protease B is such a prion, called [beta].[beta] is infectious, reversibly curable, a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1115803
更新日期:2003-09-01 00:00:00
abstract::The p53 tumor suppressor is often disrupted in human cancers by the acquisition of missense mutations. We generated mice with a missense mutation at codon 172 that mimics the p53R175H hot spot mutation in human cancer. p53 homozygous mutant mice have unstable mutant p53 in normal cells and stabilize mutant p53 in some...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1662908
更新日期:2008-05-15 00:00:00
abstract::Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.16.2469
更新日期:1998-08-15 00:00:00
abstract::Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1021002
更新日期:2002-11-01 00:00:00
abstract::The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required for early segmentat...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.328905
更新日期:2005-02-01 00:00:00
abstract::Transvection can cause the expression of a gene to be sensitive to the proximity of a homolog. It can account for many cases of intragenic complementation at the Drosophila yellow gene, where one mode of transvection involves the action of enhancers in trans on a promoter present on a separate chromosome. Our goal was...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.3.253
更新日期:1999-02-01 00:00:00