Abstract:
PURPOSE:Glutamate transporters are responsible for removing glutamate from the extracellular space and have the potential to protect neurons from excitotoxicity. In the present study, the effects of ceftriaxone and (2R, 4R)-APDC (APDC) on the protein expression of GLAST and GLT-1, the rate of glutamate uptake, and neuroprotection were evaluated in a cell culture model of glutamate excitotoxicity. METHODS:Mixed neuron/astrocyte cultures were prepared from 1 day old rat pups. Protein levels of GLAST and GLT-1 glutamate transporters were quantified using In-Cell Western techniques after acute or 5-day treatment with either ceftriaxone or APDC. Glutamate uptake was measured using Michaelis-Menten kinetics to evaluate the effects of 5-day treatment with ceftriaxone or APDC. Neuronal cell death in response to a 10-minute 1 mM glutamate challenge was measured following 5-day treatment with either ceftriaxone or APDC. RESULTS:Five-day treatment with 100 μM ceftriaxone significantly increased both GLAST and GLT-1 protein levels 31.3% and 47.5% above control, respectively, increased the Vmax 29.3%, increased the Km of glutamate uptake 117.9%, and reduced neuronal death 22.0% after a 1 mM glutamate challenge. Five-day treatment with 1 mM APDC significantly increased GLAST protein levels 27.6%, increased the Vmax 92.4%, increased the Km of glutamate transport 118.9%, and decreased neuronal death 36.8% after a 1 mM glutamate challenge. CONCLUSIONS:Chronic treatment with ceftriaxone or APDC provided neuroprotection from glutamate excitotoxicity while increasing GLAST and GLT-1 protein levels and increasing glutamate uptake. These compounds may have therapeutic potential in chronic excitotoxic neurodegenerative diseases.
journal_name
Restor Neurol Neuroscijournal_title
Restorative neurology and neuroscienceauthors
Beller JA,Gurkoff GG,Berman RF,Lyeth BGdoi
10.3233/RNN-2011-603subject
Has Abstractpub_date
2011-01-01 00:00:00pages
331-46issue
5eissn
0922-6028issn
1878-3627pii
6M57U5366203U8W2journal_volume
29pub_type
杂志文章abstract:BACKGROUND AND PURPOSE:Neuroplastic alterations of cortical excitability and activity represent the likely neurophysiological foundation of learning and memory formation. Beyond their induction, alterations of these processes by subsequent modification of cortical activity, termed metaplasticity, came into the focus of...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-2011-0601
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:High definition transcranial direct current stimulation (HD-tDCS) has been administered over single brain regions for small numbers of sessions. Safety, feasibility and tolerability of HD-tDCS over multiple brain regions, multiple daily stimulations and long periods are not established. OBJECTIVE:We studied...
journal_title:Restorative neurology and neuroscience
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journal_title:Restorative neurology and neuroscience
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abstract:PURPOSE:Previous studies demonstrated that administering extradural cortical stimulation (ECS) to rats during the acute phase of a photothrombotic infarct enhances motor recovery. However, the effect of ECS during the subacute phase was unknown. We aimed to evaluate the effects of ECS on motor recovery in a rat model o...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-140445
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abstract:PURPOSE:To analyze the effects of CGP 3466, a compound structurally related to deprenyl, on survival and function of fetal ventral mesencephalic dopaminergic neurons. METHODS:Free-floating roller tube (FFRT) cultures of rat (E14) ventral mesencephalon were treated with CGP 3466 [10-8 M] for 7 days. Tyrosine hydroxylas...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::In the present study, we have developed an animal model for central nervous system (CNS) damage using the graded crush injury of the adult rat optic nerve. This injury model has the following characteristics: (a) the injuries are reproducible; (b) the injuries can be created with controlled severity; and (c) variation...
journal_title:Restorative neurology and neuroscience
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doi:10.3233/RNN-1990-2104
更新日期:1990-01-01 00:00:00
abstract::In 2009 the German BMBF (German Ministry of Education and Research) established an Integrated Center for Research and Treatment (IFB(LMU)) of Vertigo, Balance and Ocular Motor Disorders in Munich. After the 10-year period of funding by the BMBF, it is envisioned that the (IFB(LMU)) will continue over the long term wit...
journal_title:Restorative neurology and neuroscience
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abstract:PURPOSE:The present study was aimed to investigate whether combination treatment with progesterone and rehabilitation training would be more effective than monotherapy after cerebral ischemia. METHODS:C57BL/6 mice were subjected to focal ischemia by photothrombosis and were treated with progesterone (2 mg/kg), rehabil...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-130312
更新日期:2013-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-1994-7402
更新日期:1995-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The olfactory nerve differs from cranial nerves III-XII in that it contains a specialised type of glial cell, called 'olfactory ensheathing cell' (OEC), rather than Schwann cells. In addition, functional neurogenesis persists postnatally in the olfactory system, i.e. the primary olfactory pathway continuously rebuilds...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
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doi:10.3233/RNN-120286
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:To demonstrate, in a proof-of-concept study, whether potentiating ipsilesional higher motor areas (premotor cortex and supplementary motor area) augments and accelerates recovery associated with constraint induced movement. METHODS:In a randomized, double-blinded pilot clinical study, 12 patients with chronic ...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章,随机对照试验
doi:10.3233/RNN-150574
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abstract::The antagonizing effect of the β-adrenoceptor antagonist propranolol (PRP) on the desipramine (DMI)-induced regeneration of brain noradrenergic fibers was examined. The symmetrical sites of the rat frontal cortex were pretreated with 6-hydroxydopamine (6-OHDA). Then, the same cortical site of one hemisphere was infuse...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-1994-6405
更新日期:1994-01-01 00:00:00
abstract:PURPOSE:The primary somatosensory cortex (S1) is somatotopically reorganized after limb amputation. The duration of the amputation, the intensity of phantom limb pain but also a multifactoral model of altered cerebral input have been discussed to be associated with cortical changes. Patients with finger amputation rare...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-130380
更新日期:2014-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-120228
更新日期:2013-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-160655
更新日期:2017-01-01 00:00:00
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journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-1990-13418
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:The present study was designed to investigate the neural correlates of German Sign Language (Deutsche Gebärdensprache; DGS) processing. In particular, was expected the impact of the visuo-spatial mode in sign language on underlying neural networks compared to the impact of the interpretation of linguistic infor...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::We have tested the capacity of several compounds with in vitro and/or in vivo antioxidant or antilipolytic activity to ameliorate locomotor function in cats subjected to static loading (i.e. compression) injury of the spinal cord. These include the synthetic glucocorticoid, methylprednisolone sodium succinate (MP), an...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-1991-245603
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:Transgenic manipulation of mouse physiology facilitates the preclinical study of genetic risk factors, neural plasticity, and reactive processes accompanying Alzheimer's disease. Alternatively, entorhinal cortex lesions (ECLs) model pathophysiological denervation and axonal sprouting in rat. Given reports of an...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-130311
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:In brain-computer interfaces (BCIs), electrical brain signals during motor imagery are utilized as commands connecting the brain to a computer. To use BCI in patients with stroke, unique brain signal changes should be characterized during motor imagery process. This study aimed to examine the trial-dependent mo...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-150534
更新日期:2015-08-26 00:00:00
abstract::Injury of the nervous system initiates a cascade of signal transduction including a rise in extracellular glutamate which leads to subsequent secondary neuronal loss. Excitotoxicity is known to play a crucial role in cell death spinal cord trauma. Gangliosides, particularly monosialogangliosides (GM1), are protective ...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:INTRODUCTION:Traumatic brain injuries (TBI) are one of the most common consequences of traffic accidents. Patients with mild, moderate or severe brain injuries suffer from physical, cognitive, behavioral, emotional and social problems. Most of these problems have been a long standing focus amongst practitioners and res...
journal_title:Restorative neurology and neuroscience
pub_type: 共识发展会议,杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:There is considerable variability in the extent and nature of the glial response to injury and neurodegeneration. Transplantation of fetal cortical tissue onto the brain of neonatal host rats or mice results in region-specific changes dependent on where the fetal tissue is placed. These changes include chronic ...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-150591
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:To determine how age at the time of left middle cerebral artery stroke affects language lateralization in a combined sample of subjects with perinatal, childhood, and adult stroke. METHODS:19 participants who had perinatal stroke (<1 month of age), 32 with later stroke, and 51 sex-/age-matched healthy controls...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-140402
更新日期:2014-01-01 00:00:00
abstract::A long-term goal in motor rehabilitation is that treatment is not selected on the basis of 'schools of thought', but rather, based on knowledge about efficacy and effectiveness of specific interventions for specific situations (e.g. functional syndromes). Motor dysfunction after stroke or TBI can be caused by many dif...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to delineate the relationship between several types of T1-weighted MRI pathology and motor rehabilitation potential following Constraint Induced Movement therapy (CI therapy) in chronic stroke. METHODS:Stepwise regression was employed (n = 80) to identify predictors of motor recov...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-130366
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The current treatment intervention study determined the effect of coupled bilateral training (i.e., bilateral movements and EMG-triggered neuromuscular stimulation) and resistive load (mass) on upper extremity motor recovery in chronic stroke. METHODS:Thirty chronic stroke subjects were randomly assigned to...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章,随机对照试验
doi:10.3233/RNN-2009-0455
更新日期:2009-01-01 00:00:00
abstract::Animals with unilateral neurotoxic lesions in the striatum exhibit a stereotypical biased rotational behavior in response to dopamine agonists. We recently argued that the rotational test may be subject to sensitization effects of the drug. Accordingly, we proposed the drug-free elevated body swing test (EBST) as an a...
journal_title:Restorative neurology and neuroscience
pub_type: 杂志文章
doi:10.3233/RNN-1995-9103
更新日期:1995-01-01 00:00:00