Abstract:
PURPOSE:The purpose was to investigate the effect of the superoxide dismutase mimetic tempol on decreases in retinal blood flow that are found in diabetic mice. MATERIALS AND METHODS:Streptozotocin (STZ) was injected into male C57BL/6 mice to induce hyperglycemia. One week following the STZ injection, subsets of the mice were given drinking water with or without 1 mM tempol for an additional three weeks. At the end of the four-week protocol, microvascular parameters were quantified via intravital microscopy, and included measurements of retinal diameters, red blood cell (RBC) velocities, blood flow rates, and wall shear rates. RESULTS:Diabetes induced ~40-45% decreases in retinal blood flow rate (p < 0.001) four weeks following injection of STZ. The decrease in blood flow rate occurred with decreases in microvascular diameters (D) and RBC velocities (V). The average percentage decrease in velocity was greater than the percentage decrease in diameter and, therefore, wall shear rates (= 8 V/D) were ~25% lower in the diabetics than in the non-diabetics (p < 0.05). A three-week administration of tempol in the STZ mice allowed significantly higher blood flow rates than in the untreated STZ mice, with RBC velocities improved by the antioxidant (p < 0.05 on the venular side). However, tempol provided only moderate (and not statistically significant) improvements in wall shear rates. CONCLUSIONS:The antioxidant tempol provides partial improvements in retinal microvascular hemodynamics early in the progression of STZ-induced diabetes in mice.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Yadav AS,Harris NRdoi
10.3109/02713683.2011.556300subject
Has Abstractpub_date
2011-05-01 00:00:00pages
456-61issue
5eissn
0271-3683issn
1460-2202journal_volume
36pub_type
杂志文章abstract::Reduced glutathione (0.3 mM) stimulates the activity of sodium-potassium activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione, however, has no effect on Na+K+ATPase activity in the same tissue, although it does inhibit magnesium activated ATPase...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019985
更新日期:1981-01-01 00:00:00
abstract:AIM:To investigate microscopic changes in corneal morphology in chloroquine (CQ)-treated patients without corneal abnormalities being diagnosable by slit lamp microscopy. METHODS:Thirty-six eyes of 36 patients with rheumatoid arthritis (RA) were divided into two groups: group 1 included 26 patients receiving chloroqui...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.631719
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:A quick, simple, and efficient extraction technique was developed for the removal of protein from soft hydrophilic contact lenses. METHODS:An extraction solvent consisting of a 50:50 mix of 0.2% trifluoroacetic acid and acetonitrile was used to remove protein from in vitro laboratory-deposited and human-worn c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.5.503.7049
更新日期:1997-05-01 00:00:00
abstract::The 'fast PIII' rod mediated photoreceptor response to flashes of monochromatic light has been investigated in isolated retinae of the RCS rat at age 30 days. A loss of both sensitivity and maximum response amplitude were found, with a concurrent slowing of the response kinetics. These observations indicate a rod abno...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000805
更新日期:1984-09-01 00:00:00
abstract:PURPOSE:Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular developm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1545911
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:To compare the quality of corneal endothelium of precut Descemet-stripping automated endothelial keratoplasty (DSAEK) tissue when transported with and without the anterior lamellar corneal tissue (ALCT) when organ-culture corneal storage methods are used. METHODS:After microkeratome-assisted excision of anteri...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.648045
更新日期:2012-02-01 00:00:00
abstract::An experimental model of optic nerve ischemia was designed in the rabbit to determine early biochemical alterations, i.e.--changes of high energy phosphate metabolites (ATP and phosphocreatine)--in occlusive and peri-occlusive areas. Vascular occlusion provoked a rapid fall of ATP and phosphocreatine in the optic nerv...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209001779
更新日期:1992-03-01 00:00:00
abstract::Calcium permeability was significantly increased in lenses from rats 36 h following a single injection of a cataractogenic dose of sodium selenite (30 nmol/g body weight) and this permeability returned to control values by 72 h post-injection (PI). The greater Ca2+ permeability could be partially reversed by incubatin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999466
更新日期:1993-03-01 00:00:00
abstract:PURPOSE:To evaluate the immediate effect of 3% diquafosol ophthalmic solution on tear MUC5AC concentration, periodic acid-Schiff (PAS)-positive goblet cells, and tear film stability in normal and keratoconjunctivitis sicca (KCS) rat models. METHODS:Rats were divided into normal and KCS groups. 3% of diquafosol solutio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1233986
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To examine whether transforming growth factor-beta 2 (TGF-beta 2) induces the expression of alpha-smooth muscle actin (SMA), a biochemical marker of myofibroblasts, in cultured bovine retinal pigment epithelial (RPE) cells. METHODS:Bovine RPE cells were cultured in F-12 nutrient mixture supplemented with 5% fe...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608995147
更新日期:1996-11-01 00:00:00
abstract::We measured the relative solubility of collagen in acetic acid after pepsin digestion and tentatively identified the types of collagen present in corneas of rabbits of various ages and in corneal scar tissue, using hydroxyproline assays and polyacrylamide gel electrophoretic analyses. More than 80% of the collagen in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019966
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:There is a substantial literature showing that the selective estrogen receptor modulator tamoxifen can block swelling-activated chloride channels, such as those in astrocytes. This study aimed to test the hypothesis that anatomic characteristics consistent with swelling may be measured within the optic nerve he...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600602547
更新日期:2006-04-01 00:00:00
abstract:PURPOSE:To evaluate differences of phagocytic capacities in rat retinal pigment epithelial (RPE) cells, iris pigment epithelial (IPE) cells, and basic fibroblast growth factor (bFGF) cDNA-transfected IPE (bFGF-IPE) cells in vitro. METHODS:The RPE cells and IPE cells were isolated from adult Long Evans rats' eyes. The ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.3.185.5465
更新日期:2001-09-01 00:00:00
abstract:PURPOSE:To evaluate postnatal developmental changes in the amounts of retinal glutamate, glutamine and GABA, and in the distribution of retinal glutamine in the rat. METHODS:Free amino acids were extracted from rat retinas of different postnatal stages, and the concentrations of glutamate, glutamine and GABA were dete...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609008907
更新日期:1996-06-01 00:00:00
abstract:PURPOSE:The aim of this pilot study was to determine the presence of complement activation products in tears from pre- and postkeratoplasty eyes and the fellow eyes in order to investigate the activation of the classical and alternative pathways of the complement system in the early postkeratoplasty period. METHODS:Te...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.2.99.8165
更新日期:2002-02-01 00:00:00
abstract::Purpose: To investigate to what extent the OSDI can be utilized as a discriminative test for clinical findings. Methods: One thousand and ninety patients with dry eye disease (DED) were consecutively included and examined for osmolarity, tear film break-up time (TFBUT), ocular protection index (OPI), ocular surface st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1604972
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To investigate the effect of shaking on corneal endothelial preservation. METHODS:Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into two groups. In Group ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.11.1111.5111
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:Predicting the toxic potential of compounds to the ocular surface has depended on the Draize test for the past half century. Alternatives to Draize testing have recently been sought for a number of reasons. Stress gene expression has emerged as a means of quantifying cellular reaction and, thus, the toxic poten...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.115.5325
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:This study aims to evaluate and correlate the levels of interleukin-6 (IL-6) and thrombin-antithrombin III complex (TAT) in the vitreous of patients with different vitreoretinal pathologies. METHODS:Vitreous samples were collected from 78 patients scheduled for pars plana vitrectomy at a tertiary medical cente...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1313432
更新日期:2017-09-01 00:00:00
abstract:AIMS:To elucidate the intramuscular distribution and branching patterns of the abducens nerve in the lateral rectus (LR) muscle so as to provide anatomical confirmation of the presence of compartmentalization, including for use in clinical applications such as botulinum toxin injections. METHODS:Thirty whole-mount hum...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1438631
更新日期:2018-06-01 00:00:00
abstract:PURPOSE:To determine the cellular mechanisms involved in the hypoxia-induced alteration of the retinal pigment epithelium (RPE) potentials and the light-evoked responses of the RPE in chicks. In addition, to determine the mechanisms involved in the recovery of the RPE during the post-hypoxic period. METHODS:In vitro p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713689808951219
更新日期:1998-04-01 00:00:00
abstract:PURPOSE:Oxidative damage of lens tissue contributes to the formation of age-related cataract. Pi-class glutathione-S-transferase (GSTP1) plays a role in the removal of oxidative adducts by transferring them to glutathione. To assess epigenetic regulation of GSTP1 and its potential role in age-related nuclear cataract (...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1185129
更新日期:2017-03-01 00:00:00
abstract::It has been shown that illumination of rod outer segment suspension in the presence of photosensitizers (methylene blue lambda greater than or equal to 620 nm; retinal 370 less than or equal to lambda less than or equal to 390 nm) results in chemical modification of the lipid and protein components of the photo-recept...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209019997
更新日期:1982-01-01 00:00:00
abstract::The pigmented epithelium of the vertebrate retina phagocytizes the discarded tips of photoreceptors and it is likely that a specific cellular recognition process is involved in this phenomenon. The apical surface of retinal pigmented epithelium (RPE) contains microvilli which interdigitate with the outer segment regio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034868
更新日期:1987-08-01 00:00:00
abstract:PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903509410
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:Raman spectroscopy could potentially be used as a diagnostic tool for the detection of meibomian gland dysfunction by measuring the composition, conformation, and amount of meibum lipid on the lid margin. Toward this goal and to test our studies of meibum lipid using infrared spectroscopy, we measured the Raman...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903122029
更新日期:2009-10-01 00:00:00
abstract::Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale cultures and as ascites in mice. The specificity of the antibo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000800
更新日期:1984-06-01 00:00:00
abstract::Purpose: To evaluate the correlation between pain during cataract surgery and preoperative anxiety.Methods: This prospective observational masked study included 103 eyes of 103 consecutive patients who underwent routine clear corneal incision phacoemulsification surgery at the Department of Ophthalmology, Rambam Healt...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1666996
更新日期:2020-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.2.133.5531
更新日期:2001-02-01 00:00:00
abstract::Components of the Renin Angiotensin System (RAS) have been detected in ocular tissues and fluids. The source of the ocular RAS proteins is unknown but possibilities include diffusion or leakage from the systemic circulation, specific uptake from the blood, or local synthesis. We have used RT-PCR and in situ hybridizat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409047011
更新日期:1994-10-01 00:00:00