Abstract:
:Reduced glutathione (0.3 mM) stimulates the activity of sodium-potassium activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione, however, has no effect on Na+K+ATPase activity in the same tissue, although it does inhibit magnesium activated ATPase (Mg++ATPase) by approximately 30%. Adenosine neither stimulates nor inhibits either Na+K+ATPase or Mg++ATPase in these plasma membranes. It is postulated that the stimulatory effect of glutathione on deturgescence stems from the direct reaction of the reduced form of the tripeptide on sulfhydryl groups located on plasma membranes of corneal endothelial cells. It is highly probable that these sulfhydryl groups are part of the Na+k+ATPase complex itself.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Whikehart DR,Soppet DRdoi
10.3109/02713688109019985subject
Has Abstractpub_date
1981-01-01 00:00:00pages
451-5issue
8eissn
0271-3683issn
1460-2202journal_volume
1pub_type
杂志文章abstract:PURPOSE:The purpose of these studies is to develop an in vitro model of corneal endothelial aging and to investigate age-related changes in morphology, mitosis, prostaglandin synthesis and prostaglandin response pathways. METHODS:First-passage rabbit corneal endothelial cells were grown in vitro for up to 30 days afte...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.5.428.7048
更新日期:1997-05-01 00:00:00
abstract::We adapted a previously described procedure to quantitate collagen in corneal tissue sections prepared from paraffin-embedded samples. The method entailed staining the deparaffinized tissue sections with Sirius red, eluting the bound dye with NaOH-methanol, and estimating the color in a spectrophotometer as an indicat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609015119
更新日期:1986-08-01 00:00:00
abstract:PURPOSE:To study the effect of Na-K pump and K(ATP) channel inhibition on the diameter and the adenosine-induced vasodilation of small retinal arterioles. METHODS:Thirty isolated porcine arterioles with a diameter of approximately 70 microm were mounted in a double-barrelled pipette system placed in an organ bath, and...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.1.23.9966
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:To investigate optic nerve head (ONH) pallor quantitatively in patients with primary open-angle glaucoma (POAG) and in healthy subjects, and to examine the relationship to mean deviation in perimetry (MD), cup-disk ratio (CDR), and diameters of retinal vessels. METHODS:A total of 89 POAG patients (67.6 ± 11.1 ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1307415
更新日期:2017-09-01 00:00:00
abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408995780
更新日期:1994-03-01 00:00:00
abstract:PURPOSE:To assess histopathologic prognostic factors relative to clinical ones in predicting local recurrence and survival after primary conjunctival melanoma (CM). METHODS:85 patients with CM were identified in Finland between 1967 and 2000, and 70 primary tumors were available for histopathologic study. Time to firs...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701648019
更新日期:2007-11-01 00:00:00
abstract::The cell content of the precorneal tears of eight subjects was sampled following overnight wear of a contact lens in one eye. This was compared with morning samples taken from the other eye, and afternoon samples taken several days later from both eyes. In the morning the median number of desquamated epithelial cells ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908996380
更新日期:1989-04-01 00:00:00
abstract::The morphology and postnatal ontogeny of the major pathway for aqueous outflow in the canine eye has been examined. Normal canine chamber angles of the Beagle at one day, one through ten weeks, three, four and six months, and one and two years of age were used. Angle tissue was prepared and examined by standard techni...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000791
更新日期:1984-06-01 00:00:00
abstract::The full length cDNA sequence for the lens-specific intermediate filament protein, CP49, from chicken is presented. The sequence contains features typical of the other intermediate filament proteins, including two major alpha-helical regions, helix I and II and appropriate linker regions. CP49 lacks a C-terminal non-a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508998401
更新日期:1995-07-01 00:00:00
abstract::In the bovine lens the gamma IV-crystallin fraction is a principal determinant of the phase separation and opacification temperature, Tc (Siezen et al, Proc. Natl. Acad. Sci. USA 82, 1985, 1701). We have now measured the effect on Tc of purified gamma IV-crystallin solutions produced by a variety of reagents which aff...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509003352
更新日期:1985-10-01 00:00:00
abstract::Purpose: To investigate to what extent the OSDI can be utilized as a discriminative test for clinical findings. Methods: One thousand and ninety patients with dry eye disease (DED) were consecutively included and examined for osmolarity, tear film break-up time (TFBUT), ocular protection index (OPI), ocular surface st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1604972
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To examine the effects of external modulation of epithelial-mesenchymal interaction on conjunctival epithelial cell differentiation characteristics. METHODS:Keratin K4 and laminin beta2 chain protein localization was examined in an organotypic model which facilitates the comparison of differentiation character...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.1.28.5394
更新日期:1999-01-01 00:00:00
abstract::A prototype automated flicker perimeter was used to investigate flicker luminance thresholds in glaucoma patients (n = 11), ocular hypertensives (high risk n = 10, low and intermediate risk n = 21) and normal controls (n = 10). Differential light sensitivity was measured for one eye of each subject using a flickering ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409047005
更新日期:1994-10-01 00:00:00
abstract:PURPOSE:Human beta-defensins 1 and 2 (hBD-1, -2) are antimicrobial peptides found in several epithelia including corneal epithelium. Breach of the epithelium leaves the cornea vulnerable to infection so we sought to determine if there is an increase in defensin expression after injury. METHODS:The epithelium from huma...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.1.64.6978
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:The effects of repeated topical administration of the selective beta 2-adrenergic agonist fenoterol on the intraocular pressure and on the adenylyl cyclase activity in ciliary processes in rabbit were examined in order to detect their possible causal relationship. METHODS:Intraocular pressure was measured by p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.10.1061.9020
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:To measure the cell size and cell density in five layers of the central cornea in the widely used inbred C57BL/6 mouse strain using in vivo three-dimensional (3D) two-photon (2PH) imaging. METHODS:Corneas were scanned using a 2PH laser scanning fluorescence microscope after staining with plasma membrane stain ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1031253
更新日期:2016-04-01 00:00:00
abstract::The 'fast PIII' rod mediated photoreceptor response to flashes of monochromatic light has been investigated in isolated retinae of the RCS rat at age 30 days. A loss of both sensitivity and maximum response amplitude were found, with a concurrent slowing of the response kinetics. These observations indicate a rod abno...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000805
更新日期:1984-09-01 00:00:00
abstract::The effects of light damage were studied in four inbred strains of albino rats, Fischer (F344), Lewis (L), Wistar (W), and Buffalo (BUF). Thirty-five-day-old rats (3 from each strain) were kept in cyclic light for two weeks, after which they were exposed to constant fluorescent light (180-190 foot-candles, 490-580 nm)...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999576
更新日期:1990-08-01 00:00:00
abstract::It has been shown that illumination of rod outer segment suspension in the presence of photosensitizers (methylene blue lambda greater than or equal to 620 nm; retinal 370 less than or equal to lambda less than or equal to 390 nm) results in chemical modification of the lipid and protein components of the photo-recept...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209019997
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:Statins have been shown to reduce ocular inflammation in animal models of uveitis and to prevent development of uveitis in observational studies. There have been no experimental human studies evaluating statins' efficacy and safety in uveitis. In this study, we aimed to investigate efficacy and safety of simvas...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2017.1355468
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:To investigate epithelial cell adhesion and proliferation on a newly developed elastin-like polymer (ELP) that mimics the functional characteristics of extracellular matrices. MATERIALS AND METHODS:A genetically engineered ELP with cell attachment sequences was adsorbed onto glass coverslips as 1, 2, or 3 mole...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802542053
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:The similar and overlapping activity of VEGF and the potent corneal-derived angiogenic eicosanoid 12(R)-HETrE calls for a study of the temporal relationship in the expression of these two autocoids. Since recent evidence suggests that hypoxia induces the expression of a CYP4B1 mRNA which might be involved in th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.1.1.5422
更新日期:2001-07-01 00:00:00
abstract:PURPOSE:Outbreaks of bacterial conjunctivitis have been linked to nontypeable strains of Streptococcus pneumoniae that lack a capsule, a key virulence factor for invasive infections. In contrast, isolates from sporadic, nonoutbreak cases of conjunctivitis were thought to be similar to invasive or nasopharyngeal isolate...
journal_title:Current eye research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3109/02713683.2011.624670
更新日期:2011-12-01 00:00:00
abstract:PURPOSE:To assess the potential impact of corneal crosslinking treatment (365 nm ultraviolet (UV)A irradiation with riboflavin) on the material properties of Intracorneal Rings Segments (ICRS). MATERIALS AND METHODS:Material properties were studied using FT-IR spectroscopy and UV-Vis spectroscopy. Rings were examined:...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.941071
更新日期:2015-05-01 00:00:00
abstract::Glucocorticoid-induced ocular hypertension has been demonstrated in both animals and humans. It is possible that glucocorticoid-induced changes in trabecular meshwork (TM) cells are responsible for this hypertension. In order to elaborate further the effect of glucocorticoids on the trabecular meshwork, the ultrastruc...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309020383
更新日期:1993-09-01 00:00:00
abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514055
更新日期:2018-12-01 00:00:00
abstract::Thrombospondins are a family of large multi-domain glycoproteins described as matricelluar proteins based on their ability to interact with a broad range of receptors, matrix molecules, growth factors or proteases, and to modulate array of cellular functions including intracellular signaling, proliferation and migrati...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713683.2013.877936
更新日期:2014-08-01 00:00:00
abstract:PURPOSE:The present study was aimed to investigate the expression of purinergic P2 receptors in oxygen-induced retinal neovascularization. METHODS:Immunohistochemistry was used to study the expression of purinergic P2Y2 and P2X2 receptors in the neonatal mouse retina during normal vascular development and after oxygen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701885470
更新日期:2008-03-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells ar...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract::The effects of 4% epinephrine bitartrate and 0.5% timolol on ocular and optic nerve blood flow were studied in phakic and aphakic rabbit eyes using a radioactive microsphere (85Sr) technique. Either epinephrine or timolol was given topically as eight drops in the hour immediately preceding blood flow measurements (one...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000822
更新日期:1984-10-01 00:00:00