Abstract:
PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells are reported to be one of the MMP-producing cells in the retina. However, the molecular mechanism of MMP production derived from Müller cells remains to be fully elucidated. MATERIALS AND METHODS:The human retinal Müller cell line (MIO-M1) was continuously-subcultured in high glucose (25 mM) condition. After the cells reached confluence, they were treated for 24 h with phorbol ester and/or a protein kinase C (PKC) inhibitor, GF109203X in high (25 mM) or low (5 mM) glucose condition. Gelatinase activities in conditioned medium were assessed using gelatin zymography. RT-PCR was performed to analyze the mRNA expression level of MMP-9. Western blot analysis used to detect the protein expression of tissue inhibitors of metalloproteinases (TIMPs). Electrophoresis mobility shift assay was conducted to examine transcription factors involved in MMP-9 transcription. RESULTS:We demonstrated the protein kinase C (PKC)-mediated regulation of proMMP-9 transcription and protein expression through the action of phorbol ester, an activator of PKC. Moreover, we demonstrated the expression of TIMPs, known as natural inhibitors of MMPs at the protein level in a human retinal Müller cell line for the first time, and report that production of these proteins was also regulated in a PKC-dependent manner. CONCLUSION:Our results suggest that imbalance between MMP and TIMP proteins may promote neovascularization by PKC activation in retinal Müller cells. In addition, the development of novel compounds with regulatory action on MMP and TIMP production through inhibiting PKC activity in retinal Müller cells may lead to new therapeutic approaches for the treatment and prevention of diabetic retinopathy.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Miyata Y,Kase M,Sugita Y,Shimada A,Nagase T,Katsura Y,Kosano Hdoi
10.3109/02713683.2012.682635subject
Has Abstractpub_date
2012-09-01 00:00:00pages
842-9issue
9eissn
0271-3683issn
1460-2202journal_volume
37pub_type
杂志文章abstract:PURPOSE:To examine the effects of nitric oxide synthase (NOS) inhibitors on intraocular pressure (IOP) and ocular inflammation following laser irradiation of the rabbit iris, and to investigate the involvement of nitric oxide (NO). METHODS:Thirty min after the intravenous administration of a nonselective inhibitor of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.3.308.5225
更新日期:1998-03-01 00:00:00
abstract::The morphology and postnatal ontogeny of the major pathway for aqueous outflow in the canine eye has been examined. Normal canine chamber angles of the Beagle at one day, one through ten weeks, three, four and six months, and one and two years of age were used. Angle tissue was prepared and examined by standard techni...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000791
更新日期:1984-06-01 00:00:00
abstract::Previous attempts to prepare monoclonal antibodies (MAbs) against S-antigen, a photoreceptor cell protein involved in the visual process and a potent autoantigen for the induction of experimental autoimmune uveitis (EAU), have yielded MAbs which define only carboxyl terminal epitopes. In this study we devised alternat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999622
更新日期:1990-04-01 00:00:00
abstract::Phosphatidic acid (PA) is an obligate intermediate in the phosphatidylinositol (PI) cycle and may serve as a sensitive marker for this pathway of reactions. Previously, we have shown that acetylcholine (ACh) stimulates formation of labeled PA in whole rat corneas whose phospholipids were prelabeled with [14C]arachidon...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034832
更新日期:1987-05-01 00:00:00
abstract::This study reports on serum interleukin 2-receptor (IL-2R) levels in uveitis patients. IL-2R is the mediator protein of interleukin 2 (IL-2), a mitogenetic cytokine of primarily immune cell interaction. Stimulated T-cells readily express IL-2R on their cell surface and release a soluble form into serum. The measuremen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999415
更新日期:1990-01-01 00:00:00
abstract::As it is known that chlorpromazine (CPZ) can bind to melanins as well as cause ocular phototoxicity, we investigated the cytotoxic effects of UV-visible irradiation of melanotic and amelanotic retinal pigment epithelial (RPE) cells in the presence of CPZ. At low concentrations (5 micrograms/ml) of CPZ a photosensitiza...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809047014
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:Androgens are thought to play a role in the regulation of the human lacrimal gland. Androgen receptor mRNA has been isolated from human lacrimal tissue; however, it is not known which cell(s) in human lacrimal tissue may contain androgen receptors. This study is an immunohistochemical investigation of the locat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.4.300.5362
更新日期:1999-04-01 00:00:00
abstract:PURPOSE:To assess the potential impact of corneal crosslinking treatment (365 nm ultraviolet (UV)A irradiation with riboflavin) on the material properties of Intracorneal Rings Segments (ICRS). MATERIALS AND METHODS:Material properties were studied using FT-IR spectroscopy and UV-Vis spectroscopy. Rings were examined:...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.941071
更新日期:2015-05-01 00:00:00
abstract::Purpose/Aim: The goal of this study is to reveal how age-related changes in phospholipid (PL) composition in the fiber cell plasma membranes of the human eye lens affect the cholesterol (Chol) content at which Chol bilayer domains (CBDs) and Chol crystals start to form.Materials and Methods: Saturation-recovery electr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1662058
更新日期:2020-02-01 00:00:00
abstract::Purpose: The purpose of this study is to present the study design and one month's preliminary results of a 2-year randomized trial, Combined Atropine with Orthokeratology (AOK), for myopia control study. Methods: Children aged 6 to <11 years and with 1.00-4.00 D myopia were randomly assigned to AOK group or ortho-k al...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2019.1568501
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To evaluate "in vivo" the effect of topical travoprost on the central corneal thickness (CCT) of rabbit eyes, and the changes in the CCT after acute increases of intraocular pressure (IOP) in these eyes. MATERIALS AND METHODS:This is an interventional, prospective, case-control, masked study. Topical travopros...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.608239
更新日期:2011-11-01 00:00:00
abstract::A model for endothelial transport is updated to include recent evidence. We discuss electrolyte movements based on a Na+-K+ ATPase, a Na+-H+ exchanger, a Na+-HCO3 coupler, a Cl- -HCO-3 exchanger, a K+-Cl-coupler, and K+ and anion channels. We discuss near-isotonic transport of fluid on the basis of recent findings of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509025148
更新日期:1985-04-01 00:00:00
abstract:PURPOSE:Klotho genes are expressed in limited tissues and have been found to be a pathogenic factor of age-related diseases in mammals, but their expressions and functions in human eyes are yet to be defined. This study was performed to investigate the expression of Klotho family genes in human ocular tissues and anter...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1259421
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:To determine the role of PKCγ in the regulation of gap junction coupling in the normal lens, we have compared the properties of coupling in lenses from wild type (WT) and PKC-γ knockout (KO) mice. METHODS:Western blotting, confocal immunofluorescence microscopy, immunoprecipitation, RT-PCR and quantitative rea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.573899
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To investigate the association between corneal temperature on ocular surface and stability of tear film, and define the normal reference range of corneal surface temperature in the population of Pudong New Area, Shanghai, China. METHODS:Temperature of cornea (TOC) and tear film break-up time (BUT) were measu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2016.1167920
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:In tissues outside the brain, vascular endothelial growth factor-A (VEGF) causes vascular hyper-permeability by opening of inter-endothelial junctions and induction of fenestrations and vesiculo-vacuolar organelles (VVOs). In preliminary studies, we observed that in blood-retinal barrier endothelium, other cell...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::The corneal endothelium is known to contain gap junctions on its lateral borders. Here we show that the endothelial cells of the rabbit cornea are highly dye coupled. Lucifer Yellow CH introduced into a single cell through either an intracellular glass microelectrode or a patch electrode rapidly spreads into its neigh...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909000876
更新日期:1989-08-01 00:00:00
abstract::It has been hypothesized that an activator of Ca-ATPase co-exists with Ca-ATPase in the mouse lens. Though the Ca-ATPase activity could be measured from mouse lens homogenate, its activity could not be determined in individual soluble and insoluble fractions. The Ca-ATPase activity, however, could be detected when an ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409065593
更新日期:1984-05-01 00:00:00
abstract:PURPOSE:To evaluate the effect of the birth weight and the postconceptional age on the tear production of preterm and term newborn infants and to evaluate the changes in tear production during the first two months of life. SUBJECTS--METHODS: Both eyes of medically stable term and preterm infants were included in the st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.28.2.145.26235
更新日期:2004-02-01 00:00:00
abstract::Four days after corneal inoculation of mice with herpes simplex (type 1) virus (HSV), infected trigeminal ganglion cells with and without calcitonin gene-related peptide (CGRP) antigenicity were examined by electron microscopy in sections treated with colloidal gold labeled antibodies. Cells that contain CGRP were ide...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020354
更新日期:1991-01-01 00:00:00
abstract::Purified Pseudomonas aeruginosa elastase cleaved a 65 kDa gelatinase [inactive proenzyme form of matrix metalloproteinase (MMP-2)] from human corneal fibroblasts into a biologically active fragment with an approximate molecular mass of 58 kDa. However, purified pseudomonal alkaline protease did not cleave MMP-2 apprec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209015082
更新日期:1992-11-01 00:00:00
abstract::The immunochemical reactivities of the alpha A and alpha B subunits from bovine alpha-crystallin have been compared using 5 monoclonal antibodies and 2 polyvalent antisera. Each subunit bound the same maximum amount of antibody, regardless of its source, and each subunit was able to completely displace alpha-crystalli...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609020047
更新日期:1986-03-01 00:00:00
abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001869
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To investigate the therapeutic role of topical and oral fluconazole treatment using a rabbit model of Aspergillus fumigatus keratitis. METHODS:Aspergillus fumigatus spores were injected into the corneal stroma of the right eye of 20 rabbits. Forty-eight hours later the rabbits were randomly divided into three ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.2.113.14514
更新日期:2003-02-01 00:00:00
abstract::The effect of cyclosporine (CsA) treatment in both primary and secondary Chlamydia trachomatis ocular infection in cynomolgus monkeys was studied. Following primary infection, CsA-treated animals developed severe clinical symptoms which persisted for up to 12 weeks compared to control animals which developed less seve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034831
更新日期:1987-05-01 00:00:00
abstract:PURPOSE:The pneumococcal capsule is required for pathogenesis in systemic infections, yet reports show most conjunctivitis outbreaks are caused by nonencapsulated pneumococci, while keratitis infections are caused by encapsulated strains. This study aims to determine the effect of capsule in pneumococcal keratitis and ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.492462
更新日期:2010-09-01 00:00:00
abstract::Ocular aldehyde oxidase was purified for the first time from bovine ciliary body cytosol by ammonium sulfate fractionation and successive HPLC using DEAE anion-exchange and hydroxyapatite columns. The purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The mol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909025807
更新日期:1989-07-01 00:00:00
abstract:PURPOSE:To evaluate how patient satisfaction after cataract surgery is associated with postoperative visual acuity, visual functioning, dry eye signs and symptoms, health anxiety, and depressive symptoms. PATIENTS AND METHODS:Fifty-four patients (mean age: 68.02 years) were assessed 2 months after uneventful phacoemul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1262429
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular developm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1545911
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:To investigate whether zeaxanthin, the predominant carotenoid pigment of the macular pigments in human retina, provides neuroprotection against retinal cell damage. METHODS:We used in vitro cultured retinal ganglion cells (RGCs), specifically RGC-5, an E1A virus-transformed rat cell line. Cell damage was induc...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680902745408
更新日期:2009-04-01 00:00:00