Abstract:
:Ocular aldehyde oxidase was purified for the first time from bovine ciliary body cytosol by ammonium sulfate fractionation and successive HPLC using DEAE anion-exchange and hydroxyapatite columns. The purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of the enzyme was estimated to be about 150,000 by electrophoresis and to be about 300,000 by gel filtration HPLC on a TSK gel G3000SWXL column, indicating that the enzyme consists of two subunits with the same molecular weight. On the other hand, nicotinamide N-oxide reductase activity was associated with aldehyde oxidase activity throughout the purification steps of the latter enzyme. This fact indicated that nicotinamide N-oxide reductase activity of the ciliary body cytosol is due to aldehyde oxidase present in the tissue preparation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Shimada S,Mishima HK,Nikaido H,Kitamura S,Tatsumi Kdoi
10.3109/02713688909025807subject
Has Abstractpub_date
1989-07-01 00:00:00pages
721-6issue
7eissn
0271-3683issn
1460-2202journal_volume
8pub_type
杂志文章abstract::The lacrimal gland stores retinyl esters which are synthesized by the enzyme acyl CoA:retinyl acyl transferase. Retinol is released from retinyl ester reserves by retinyl ester hydrolase (REH). Since the lacrimal gland secretes retinol, this gland should also contain this enzyme. To identify bile salt-dependent REH ac...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209033486
更新日期:1992-09-01 00:00:00
abstract::Water-soluble (WS) and water-insoluble (WI) crystallins were prepared from calf and bovine lenses. The WS-crystallins were analyzed by capillary isotachophoresis and by immunoelectrophoresis with polyvalent and alpha-, beta-, gamma-crystallin specific antisera. The WI-crystallins were first solubilized in formamide an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409065590
更新日期:1984-05-01 00:00:00
abstract::Purpose: The optical coherence tomography (OCT) has been used to evaluate the changes of retinal degeneration in patients with diabetic peripheral neuropathy (DPN) in recent years, but the results of previous studies were controversial. Therefore, systematic review and meta-analysis were performed to evaluate the degr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2021.1874025
更新日期:2021-01-11 00:00:00
abstract::Four groups of BALB/c mice were treated locally with different concentrations (0.1-1.0%) of retinoic acid (Etretinate) ointment for a period of one to four weeks. A fifth group was treated with the ointment base only. Ia-positive dendritic cells were identified by an indirect immunofluorescence technique which was con...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689509033511
更新日期:1995-03-01 00:00:00
abstract:PURPOSE:To evaluate the clinical outcomes of laser in situ keratomileusis (LASIK) using a new approach for the calculation of the ablation profile based on wavefront vertexing from pupil plane to corneal. METHODS:One hundred eyes of 50 patients (age, 21-41 years) with low and moderate myopia were enrolled in this pros...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1822418
更新日期:2020-09-16 00:00:00
abstract:PURPOSE:To study the effect of cataract surgery with intraocular lens (IOL) on frequency doubling perimetry (FDP). METHODS:Patients aged 40 years or above seen at our outpatient clinic with no ocular pathology except for visually significant cataract and visual acuity 6/24 or better were eligible. They underwent FDP b...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490904278
更新日期:2005-02-01 00:00:00
abstract:PURPOSE:To determine retinal vessel diameter variations in response to neuronal activity induced by diffuse luminance flicker. METHODS:The diameter of retinal arteries and veins was measured in 9 normal subjects by computer analysis of fundus pictures taken in monochromatic light under normal conditions of illuminatio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.12.1252.5021
更新日期:1997-12-01 00:00:00
abstract:PURPOSE:This study was designed to investigate whether synthetic small interference RNA (siRNA) targeting VEGF-A could inhibit mouse corneal neovascularization or not. METHODS:First, the effect of synthetic siRNA targeting VEGF-A on the expression of VEGF in human retinal pigment epithelial line ARPE-19 cells was exam...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003597230
更新日期:2010-05-01 00:00:00
abstract::The water soluble (WS), urea soluble (US) and urea insoluble (UI) fractions from individual human lenses 1.8 to 65 years of age were isolated from concentric fiber layers. In lenses younger than 19 years, a uniform distribution in the amount of WS, US and UI fractions was found throughout the entire lens. These fracti...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609015101
更新日期:1986-02-01 00:00:00
abstract:PURPOSE:The aim of the present study was to evaluate the efficacy of the inverted internal limiting membrane (ILM) flap technique that involved the use of a perfluorocarbon liquid (PFCL) bubble for the selective prevention of indocyanine green (ICG) staining of the parts of the inverted flap in contact with the retinal...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1524014
更新日期:2019-01-01 00:00:00
abstract::Cornea endothelial cells sensitized with rose bengal and exposed to light demonstrated a wavelength dependent alteration in physiological response. Corneas exposed to 549 millimicron swelled at rates similar to controls exposed to identical energy levels of incandescent light. Corneas exposed to 451, 500, 612, and 651...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019990
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To determine the roles of the murine interleukin-8 receptor homolog (mIL-8Rh, neutrophil chemokine CXC receptor 2) and macrophage inflammatory protein-1alpha (MIP-1alpha, a CC chemokine) in two eye inflammation models: endotoxin-induced uveitis (EIU) and immune complex-induced uveitis (reverse passive Arthus re...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.1.76.5339
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVES:1) To elucidate the role of collector channels in the aqueous humor outflow pathway 2) To suggest anatomic and functional methods of imaging collector channels in-vitro and in-vivo and 3) To discuss the role of such imaging modalities in the surgical management of glaucoma. METHODS:A thorough literature sea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1773866
更新日期:2020-10-01 00:00:00
abstract:PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903509410
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:To investigate the effects of citicoline on upregulated clusterin and retinal damage induced by kainic acid (KA). METHODS:KA was injected into the vitreous of rats. Effects of systemic citicoline treatments were estimated by measuring the thickness of the various retinal layers, immunoblotting, and immunohisto...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701758719
更新日期:2007-12-01 00:00:00
abstract::Purpose/Aim: The goal of this study is to reveal how age-related changes in phospholipid (PL) composition in the fiber cell plasma membranes of the human eye lens affect the cholesterol (Chol) content at which Chol bilayer domains (CBDs) and Chol crystals start to form.Materials and Methods: Saturation-recovery electr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1662058
更新日期:2020-02-01 00:00:00
abstract::The pigmented epithelium of the vertebrate retina phagocytizes the discarded tips of photoreceptors and it is likely that a specific cellular recognition process is involved in this phenomenon. The apical surface of retinal pigmented epithelium (RPE) contains microvilli which interdigitate with the outer segment regio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034868
更新日期:1987-08-01 00:00:00
abstract:PURPOSE:Corneal scarring is a common poor outcome of corneal trauma. Transforming growth factor β-1 plays a vital role in corneal fibrosis, inducing keratocyte transformation to myofibroblasts. Other than corneal transplantation, no other curative treatment methods for corneal scarring are currently available. Hypercap...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1820526
更新日期:2020-09-11 00:00:00
abstract:PURPOSE:To assess histopathologic prognostic factors relative to clinical ones in predicting local recurrence and survival after primary conjunctival melanoma (CM). METHODS:85 patients with CM were identified in Finland between 1967 and 2000, and 70 primary tumors were available for histopathologic study. Time to firs...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701648019
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:To evaluate the ultrastructure of the cut edge and associated endothelial cell loss following donor cornea trephination with a standard punch, vacuum punch, and vacuum trephine and artificial anterior chamber system. MATERIALS AND METHODS:This laboratory investigation compared trephinations (8.0 mm) performed ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903222779
更新日期:2009-11-01 00:00:00
abstract::Systemically administered fluorescein is converted to fluorescein glucuronide. The conjugate is fluorescent and interferes with the measurement of fluorescein in the anterior chamber. Carboxyfluorescein is a hydrophilic derivative of fluorescein. In the rabbit, carboxyfluorescein is not converted as readily to a fluor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609025171
更新日期:1986-05-01 00:00:00
abstract::Purpose/Aim: To assess the changes in anterior eye segment biometrics and intraocular pressure during and after wearing swimming goggles.Materials and methods: Anterior segment biometry and intraocular pressure were assessed in 20 healthy young adults (22.2 ± 4.7 years) during the wearing of a drilled swimming goggle....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1662056
更新日期:2020-05-01 00:00:00
abstract:PURPOSE:We report on the use of subconjunctival bevacizumab on corneal vessel density in recurrent pterygia. METHODS:The charts of 5 patients with recurrent pterygium, who received subconjunctival injections of bevacizumab (2.5 mg/0.1 ml) were retrospectively reviewed. Ophthalmic evaluation included Snellen visual acu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701799101
更新日期:2008-01-01 00:00:00
abstract::Fresh isolates of neonatal human corneal endothelial cells were maintained in tissue culture using a technique employing collagen-coated, dextran-based microcarrier beads. This method provides large yields of endothelial cells suitable for biochemical and/or transplant studies without exposing the cells to enzymatic p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689009000051
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:Increases in matrix proteins, such as type I collagen and fibronectin, are observed with aging in the retinal pigment epithelium (RPE) basement membrane. However, little is known about altered gene expression profiles of RPE associated with increases in matrix proteins. We investigated changes in gene expressio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701604749
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate how glutathione reductase (GR) loses its activity during cataract formation and whether it is possible to revive it back to the normal levels. METHOD:In this study, endogenous as well as synthetic reducing systems (GSH, TTase, DTT, captopril) and alpha-crystallin at dif...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590953110
更新日期:2005-10-01 00:00:00
abstract:PURPOSE:N-acetyl-cysteine (NAC) is a potent antioxidant known to be a precursor of glutathione. The purpose of this study was to investigate the role of NAC in the development of choroidal neovascularization (CNV). METHODS:CNV was induced in C57BL/6 mice by laser photocoagulation of the ocular fundus. Mice were inject...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.500112
更新日期:2010-11-01 00:00:00
abstract::We have either introduced into the vitreous space or perfused through the anterior chamber of macaque monkey eyes two anionic tracers, anionic ferritin (AF) and horseradish peroxidase (HRP) and a cationic probe, cationic ferritin (CF). We have observed that the anionic molecules, but not the cationic one, are transpor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509025167
更新日期:1985-04-01 00:00:00
abstract::The initial rapid fall in tear film fluorescence after instilling a 1 microliter drop of fluorophore could be a result of stimulated tear flow or of distribution of the dye throughout the tear fluid present in the conjunctival sac. An attempt was made to decide this by comparing the fall in fluorescence of two differe...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689509033526
更新日期:1995-04-01 00:00:00
abstract::Change of urate and ascorbate levels in retinae of rat left eyes after ophthalmic artery and optic nerve ligation was studied by reversed-phase high-performance liquid chromatography with electrochemical detection. The right eyes were used for sham operation. We found that uric acid levels in the retinae of the left e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608996369
更新日期:1986-07-01 00:00:00