Abstract:
PURPOSE:N-acetyl-cysteine (NAC) is a potent antioxidant known to be a precursor of glutathione. The purpose of this study was to investigate the role of NAC in the development of choroidal neovascularization (CNV). METHODS:CNV was induced in C57BL/6 mice by laser photocoagulation of the ocular fundus. Mice were injected intraperitoneally with NAC or vehicle alone. The levels of 4-hydoroxy-2-nonenal (4-HNE)-modified protein and nucleus factor (NF)-κB were determined by wester blotting. The recruitment of macrophages and neutrophils after laser injury was analyzed immunohistochemically and in myeloperoxidase (MPO) assays. Enzyme-linked immunosorbent assays (ELISA) were used to measure monocyte chemotactic protein (MCP)-1, CXCL1, vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-1, and VEGFR-2. The extent of CNV was evaluated 7 d after laser injury by lectin staining. RESULTS:In NAC-treated mice with laser-induced injuries, the induction of 4-HNE-modified protein after 3 hr and the activation of NF-κB in nuclear extracts after 6 hr were markedly suppressed compared to vehicle-treated mice. Macrophage and neutrophil recruitment were inhibited and the levels of MCP-1, CXCL1, VEGF, and VEGFR-1 were also lower in NAC-treated mice compared to vehicle-treated mice. Furthermore, the extent of CNV induced was significantly lower in NAC-treated compared to vehicle-treated mice (p = 0.027). CONCLUSIONS:Our results clearly showed that NAC inhibited indicators of oxidative stress and the activation of NF-κB induced by laser injury, and, consequently, suppressed macrophage and neutrophil infiltration and the development of CNV. This suggests novel preventative and interventional therapeutic strategies for age-related macular degeneration.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Hara R,Inomata Y,Kawaji T,Sagara N,Inatani M,Fukushima M,Tanihara Hdoi
10.3109/02713683.2010.500112subject
Has Abstractpub_date
2010-11-01 00:00:00pages
1012-20issue
11eissn
0271-3683issn
1460-2202journal_volume
35pub_type
杂志文章abstract:PURPOSE:The purpose of this work is to describe and review the technology of plasma rich in growth factors (PRGF), a novel blood derivative product, in the treatment of ocular surface disorders. METHODS:To demonstrate the importance of this technology in the treatment of ocular pathologies, a thorough review of the pr...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713683.2015.1104362
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:To evaluate clinical outcome(s) and complication(s) of topical bevacizumab (avastin) in patients with impending recurrent pterygium. PATIENTS AND METHODS:This prospective clinical trial included 54 consecutive patients (54 eyes), who underwent pterygium surgery with bare sclera and mitomycin, and who were diag...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.3109/02713680903395273
更新日期:2010-01-01 00:00:00
abstract::Due to the multiple barriers imposed by the eye against the penetration of drugs, the ocular delivery and targeting are considered difficult to achieve. A major challenge in ocular drug therapy is to improve the poor bioavailability of topically applied ophthalmic drugs by overcoming the severe constraints imposed by ...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713681003760168
更新日期:2010-07-01 00:00:00
abstract:PURPOSE:Several previous studies have shown that glycoconjugates of extracellular matrix, cell membrane and nucleus play an important role in the mediation of cell proliferation, migration and differentiation. Lens epithelial cells and lens fiber cells show regional differences with regard to these parameters. If glyco...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.6.527.5083
更新日期:1997-06-01 00:00:00
abstract::Corneal acidosis has been shown to reduce corneal hydration control (CHC) as measured by the rate, expressed as the percent recovery per hour (PRPH), at which the thickness of the cornea decreases exponentially after an increased hydration load. Since the effect of pH on corneal function is of scientific interest and ...
journal_title:Current eye research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/02713689508999932
更新日期:1995-05-01 00:00:00
abstract:PURPOSE:We performed nailfold capillary microscopy to explore microvasculature abnormalities in uveitis overall and uveitis stratified in various ways. METHODS:This was a cross-sectional, case-control, observational study. One hundred and seven uveitis patients and 130 control subjects were included. We used a JH-1004...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1496265
更新日期:2018-11-01 00:00:00
abstract::This study asks whether retinal adhesiveness is affected by cytochalasin D, a drug that is known to alter the apical morphology of the retinal pigment epithelium (RPE). Cytochalasin D was injected intravitreally in Dutch rabbits and retinal adhesiveness measured 0.5 to 72 h later by two methods: in vitro peeling of th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508995817
更新日期:1995-12-01 00:00:00
abstract:PURPOSE:To synthesize and characterize hydrogels with viscoelastic properties comparable to those of the natural lens. METHODS:Hydrogels were synthesized in water by free-radical polymerization of the monomer poly(ethyleneglycol)-monomethacrylate. Three different molecular weights of poly(ethyleneglycol)-dimethacrylat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.5.384.5493
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:Pseudoexfoliation (PXF) is a microfibrillopathy involving disordered elastogenesis. Abnormal extracellular matrix (ECM) production underlies the pathophysiology of PXF. The enzyme Lysyl oxidase (LOX) and its isoforms are known to cross-link the elastin and collagen. Though the etiopathogensis of PXF is not well...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1125505
更新日期:2016-10-01 00:00:00
abstract:PURPOSE:To evaluate the ultrastructure of the cut edge and associated endothelial cell loss following donor cornea trephination with a standard punch, vacuum punch, and vacuum trephine and artificial anterior chamber system. MATERIALS AND METHODS:This laboratory investigation compared trephinations (8.0 mm) performed ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903222779
更新日期:2009-11-01 00:00:00
abstract:PURPOSE:Dry eye is a common condition known to have many systemic co-morbidities. We aim to report the frequency of depression and anxiety suspects in eye clinic patients and explore the association between dry eye signs and symptoms with depression and anxiety. MATERIALS AND METHODS:Adult outpatients were recruited f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1056804
更新日期:2016-05-01 00:00:00
abstract::The effect of cytochalasin D (CD), an actin monomer-stabilizer, has been studied on cataract development in rat lenses. Cataractogenesis was induced by incubating the rat lenses in medium 199 (M199) containing 10(-5) M CD; by the end of 24 h, lenses first developed a visible opacity. The increased lactate dehydrogenas...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508995134
更新日期:1995-10-01 00:00:00
abstract::Purpose/Aim: Corneal abrasions and nonhealing corneal epithelial defects are common conditions that cause pain and sometimes are slow to heal. Histatins, a family of histidine-rich peptides, have been implicated in oral and skin epithelial wound healing, and have been shown to be effective in vitro in human corneal ep...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1490772
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:Previous studies have suggested that corneal fibrosis controlled by a TGFbeta-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFbeta in post-PR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::In the present study, using retinal ischemia as a model, we examined if different periods of ischemia and recirculation influenced the generation of reactive oxygen species, i.e. in hydrogen peroxide generation and nitroblue tetrazolium (NBT) reduction. Ischemia was induced for 30 and 90 min by ligation of the optic n...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508999913
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.669005
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:Taurolidine is a broad-spectrum, non antibiotic antimicrobial agent, not previously tested against the common causes of bacterial keratitis. This study, employing an experimental rabbit model of Staphylococcus aureus keratitis, investigated the effectiveness of topical taurolidine in reducing the number of bact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490908733
更新日期:2005-03-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) were used to model the solar radiation-induced cataract that occurs in hatchery-reared lake trout (Salvelinus namaycush). Rainbow trout eyes were exposed to wideband predominantly UVB radiant energy from a filtered 1000W xenon source. Lenticular damage was assessed and classified an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999477
更新日期:1993-02-01 00:00:00
abstract:PURPOSE:Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a member of the TIMP family of proteins, playing a significant role in the control of extracellular matrix remodelling. TIMP-3 might play a role in the regulation of retinal neovascularization during progression of diabetic retinopathy. Recently, three novel p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.91.15954
更新日期:2003-08-01 00:00:00
abstract:PURPOSE:Intravitreal injection of ciliary neurotrophic factor (CNTF) is known to induce glial intermediate filament protein (GFAP) expression in retinal Müller cells. Because CNTF binding can activate multiple signaling kinases, we have examined the involvement of JAK/STAT pathway in GFAP induction in Müller cells. ME...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.4.305.8408
更新日期:2002-04-01 00:00:00
abstract:PURPOSE:This study was carried out in order to determine the most potent and novel uveitopathogenic sites of recoverin using synthetic peptides. METHODS:Several synthetic peptides containing the recoverin sequence plus adjuvants were injected into Lewis rats, and the uveitopathogenic sequence was defined, clinically, ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.4.304.5503
更新日期:2001-04-01 00:00:00
abstract:PURPOSE:The aim of this study was to characterize swelling pressure-thickness, swelling pressure-hydration and hydration-thickness relations of porcine cornea. METHODS:Mechanical compression tests and free swelling experiments were performed on porcine cornea. A rheometer (DHR-2, TA Instruments) with a thermally contr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.809769
更新日期:2013-11-01 00:00:00
abstract:PURPOSE:To characterize the effects that mode of sampling and overnight eye closure have on the nature of caseinolytic activity recovered in tear fluid. METHODS:Reflex, open and closed (R, O and C) eye tear fluids were collected by microcapillary tubes or from the inferior formix by Schirmer strip. Microcapillary coll...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.8.810.8992
更新日期:1997-08-01 00:00:00
abstract::The variation of central corneal thickness with age was measured weekly at 10am by ultrasound pachometry in 10 normal (5 to 19 weeks of age) and 10 alloxan-induced diabetic (9 to 19 weeks of age) rabbits. The diurnal variation of corneal thickness was also measured at 2 hour intervals for a period of 24 hours on both ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999611
更新日期:1990-05-01 00:00:00
abstract:PURPOSE:To test if nicotine counteracts the dampening effect of human cytomegalovirus (HCMV) infection of NF-kappaB in retinal pigment epithelial (RPE) cells, thereby increasing the permissiveness of RPE cells for HCMV replication. METHODS:Human ARPE-19 cells were transfected with NF-kappaB luciferase DNA, inoculated ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500514651
更新日期:2006-02-01 00:00:00
abstract:PURPOSE:To evaluate the effect of gatifloxacin ophthalmic solution 0.3% (Zymar, Allergan, Inc., Irvine, CA, USA) on corneal endothelial cell density and morphology and to measure gatifloxacin penetration into aqueous humor. METHODS:This was a single-center, open-label clinical study. Ten patients undergoing standard c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590968556
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:To investigate the effect of shaking on corneal endothelial preservation. METHODS:Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into two groups. In Group ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.11.1111.5111
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:To investigate whether retinal impairment was affected by age of rats in acute glaucoma model. METHODS:Young adult and aged rats were randomly divided into normal control, 45 mmHg, 60 mmHg and 90 mmHg groups. Intraocular pressures (IOP) of rats were acutely elevated to 45 mmHg, 60 mmHg and 90 mmHg, respectivel...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.922194
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:To evaluate the clinical outcomes of laser in situ keratomileusis (LASIK) using a new approach for the calculation of the ablation profile based on wavefront vertexing from pupil plane to corneal. METHODS:One hundred eyes of 50 patients (age, 21-41 years) with low and moderate myopia were enrolled in this pros...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1822418
更新日期:2020-09-16 00:00:00
abstract::Purpose/Aim: Many genes have been associated with primary open-angle glaucoma (POAG). Knowing exactly where they are expressed in the eye helps to unravel POAG pathology and to select optimal targets for intervention. We investigated whether RNA in situ hybridization (RNA-ISH) is a convenient technique to obtain detai...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1607392
更新日期:2019-09-01 00:00:00