Vitamin C reduces cytochalasin D cataractogenesis.

Abstract:

:The effect of cytochalasin D (CD), an actin monomer-stabilizer, has been studied on cataract development in rat lenses. Cataractogenesis was induced by incubating the rat lenses in medium 199 (M199) containing 10(-5) M CD; by the end of 24 h, lenses first developed a visible opacity. The increased lactate dehydrogenase (LDH) activity in the culture medium, leakage of lens cytosolic proteins into the culture medium and observable development of opacity through a dissection microscope were correlated with cell damage associated with cataract formation. Non-denaturing polyacrylamide gel electrophoresis was used to separate three lens LDH isoenzymes. The effect of 1 mM vitamin C (VC) in reducing LDH leakage was also examined. The protective effect of VC on CD-initiated cataractous lenses is significant. This suggest that a portion of the opacity and lens damage may involve oxidative damage to the membrane-cytoskeleton complex which is started by CD, but partially prevented by VC

journal_name

Curr Eye Res

journal_title

Current eye research

authors

Kilic F,Trevithick JR

doi

10.3109/02713689508995134

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

943-9

issue

10

eissn

0271-3683

issn

1460-2202

journal_volume

14

pub_type

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