Abstract:
PURPOSE:Intravitreal injection of ciliary neurotrophic factor (CNTF) is known to induce glial intermediate filament protein (GFAP) expression in retinal Müller cells. Because CNTF binding can activate multiple signaling kinases, we have examined the involvement of JAK/STAT pathway in GFAP induction in Müller cells. METHODS:CNTF was injected intravitreally into mouse eyes. Immunocytochemistry and immunoblotting were used to study GFAP and STAT3-p (phosphorylated STAT3) levels either in mouse eyes, retinal explant cultures or in a Müller cell line, rMC-1. RESULTS:In protein extracts of CNTF-injected eyes, retinal explants and the Müller cells, there was a substantial increase in STAT3-p level. Immunocytochemistry showed that STAT3-p was now present in many cell bodies in the INL and the GCL. To prove that CNTF acted via the JAK-STAT pathway, rMC-1 cells were transfected with a dominant-negative STAT3 mutant prior to treatment with CNTF. In the immunoblots of transfected cells, there was decrease in GFAP level. CONCLUSIONS:The results establish that CNTF can induce GFAP expression in retinal Müller cells through the JAK/STAT signaling pathway.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Wang Y,Smith SB,Ogilvie JM,McCool DJ,Sarthy Vdoi
10.1076/ceyr.24.4.305.8408subject
Has Abstractpub_date
2002-04-01 00:00:00pages
305-12issue
4eissn
0271-3683issn
1460-2202journal_volume
24pub_type
杂志文章abstract::Purpose/Aim: The goal of this study is to reveal how age-related changes in phospholipid (PL) composition in the fiber cell plasma membranes of the human eye lens affect the cholesterol (Chol) content at which Chol bilayer domains (CBDs) and Chol crystals start to form.Materials and Methods: Saturation-recovery electr...
journal_title:Current eye research
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doi:10.1080/02713683.2019.1662058
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journal_title:Current eye research
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journal_title:Current eye research
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abstract::Uveal melanoma develops in one of the most capillary-rich tissues of the body and is disseminated hematogenously. Knowledge of the nature and the spatiotemporal expression of angiogenic factors in uveal melanoma is essential to the development of new treatment strategies, especially with regard to improving survival. ...
journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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doi:10.3109/02713688409167214
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journal_title:Current eye research
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journal_title:Current eye research
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abstract::Ocular aldehyde oxidase was purified for the first time from bovine ciliary body cytosol by ammonium sulfate fractionation and successive HPLC using DEAE anion-exchange and hydroxyapatite columns. The purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The mol...
journal_title:Current eye research
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journal_title:Current eye research
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