Abstract:
:Uveal melanoma develops in one of the most capillary-rich tissues of the body and is disseminated hematogenously. Knowledge of the nature and the spatiotemporal expression of angiogenic factors in uveal melanoma is essential to the development of new treatment strategies, especially with regard to improving survival. In this study, we measured the angiogenic potential of several angiogenic factors in different uveal melanoma cell lines, in an in vivo model, and in primary tumor material from patients with melanoma. Most uveal melanoma cell lines expressed vascular endothelial growth factor (VEGF)-A (isoforms 121, 165, 189), VEGF-B, VEGF-C, VEGF-D, and basic fibroblastic growth factor (b-FGF) to various extents. The expression of VEGF-A 121 was always higher than that of the other VEGF-A isoforms, suggesting that VEGF-A 121 is the most abundant VEGF-A isoform. All experimentally induced tumors expressed VEGF-A, VEGF-B, VEGF-C, VEGF-D, and basic fibroblastic growth factor (b-FGF). Similarly, significant amounts of mRNA for VEGF-B, VEGF-C, VEGF-D, and b-FGF were detected in uveal melanoma material from patients. In contrast, VEGF-A mRNA (121, 165, 189) was low (9/28) or not detectable in the tumor samples. The synthesis of VEGF-A 165 and b-FGF protein by various cell lines was measured by enzyme-linked immunosorbent assay (ELISA). Most uveal melanoma cell lines, but not normal melanocytes, strongly synthesized and secreted VEGF-A 165 and b-FGF during cell culture. Our data suggest that the expression of (lymph) angiogenic factors may play a causal role in the angiogenesis and progression of uveal melanoma and distant metastasis.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Notting IC,Missotten GS,Sijmons B,Boonman ZF,Keunen JE,van der Pluijm Gdoi
10.1080/02713680600865052subject
Has Abstractpub_date
2006-09-01 00:00:00pages
775-85issue
9eissn
0271-3683issn
1460-2202pii
W64260G5K2036116journal_volume
31pub_type
杂志文章abstract:PURPOSE:To study the relationship of oxygen level and glucose concentration on the secretion of vascular endothelial growth factor (VEGF) mRNA and protein in several types of cultured retinal cells. MATERIALS AND METHODS:Several types of human and bovine retinal cells were cultured in medium without glucose, or contai...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.521968
更新日期:2011-02-01 00:00:00
abstract::In uveal vessels of isolated arterially perfused rabbit eyes there is direct evidence for the presence of post-junctional alpha 1-receptors and indirect evidence for alpha 2-receptors. Since vasoconstriction by epinephrine could not be blocked by an alpha 1-antagonist, only 30% by an alpha 2-antagonist and almost full...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688508999963
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:To demonstrate spectroscopic retinal oximetry measurements on arteries and veins in swine using intravitreal illumination. Retinal arterial and venous saturations are measured for a range of inspired O2 levels after pars plana vitrectomy. METHODS:Pars plana vitrectomy and intravitreal manipulations were perfor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600760493
更新日期:2006-07-01 00:00:00
abstract:PURPOSE/AIM:We aimed to characterize the connective tissue microanatomy, elastin abundance, and fiber orientation in the human optic nerve sheath, also known as the optic nerve dura mater, for correlation with its biomechanical properties. MATERIALS AND METHODS:Seven whole human orbits aged 4-93 years, and five isolat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1739314
更新日期:2020-07-01 00:00:00
abstract:PURPOSE:Recent advances in technology now provide tools capable of tracking genome-wide expression changes occurring in progressive pathological processes. The present experiments were carried out to determine if acetylcholine receptor α 6 subunit (Chrna6) is a reliable retinal ganglion cell (RGC) marker in adult mouse...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.724512
更新日期:2013-01-01 00:00:00
abstract::The distribution of vasoactive intestinal polypeptide (VIP)- and neurotensin (NT)-like immunoreactivities in the chicken retina was investigated by immunohistochemistry. VIP- and NT-positive cells were found throughout the chicken retina. The majority of them were located in the proximal portion of the inner nuclear l...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001736
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To determine the cellular mechanisms involved in the hypoxia-induced alteration of the retinal pigment epithelium (RPE) potentials and the light-evoked responses of the RPE in chicks. In addition, to determine the mechanisms involved in the recovery of the RPE during the post-hypoxic period. METHODS:In vitro p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713689808951219
更新日期:1998-04-01 00:00:00
abstract:PURPOSE:To study the effect of Na-K pump and K(ATP) channel inhibition on the diameter and the adenosine-induced vasodilation of small retinal arterioles. METHODS:Thirty isolated porcine arterioles with a diameter of approximately 70 microm were mounted in a double-barrelled pipette system placed in an organ bath, and...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.1.23.9966
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:Phoria is used in the diagnosis of binocular dysfunctions, such as convergence insufficiency. A common treatment for convergence insufficiency is vision therapy, yet it is controversial whether the phoria changes as a result of rehabilitation. Phoria can vary due to the duration and/or the type of prior visual ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.694551
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:To investigate the association of nailfold capillaroscopy, heart rate variability (HRV), and clinical characteristics of glaucoma with the plasma matrix metalloproteinase-9 (MMP-9) level in normal-tension glaucoma (NTG). MATERIALS AND METHODS:We conducted a prospective, cross-sectional study on 25 patients wit...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.971937
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To establish autologous iris pigment epithelial (IPE) cell transplantation in monkey eyes. METHODS:Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cell culture was performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To determine whether substance P is present in human tears. METHODS:Tear samples (1-2 microliters) were collected from one eye of each of 12 subjects. Two of the eyes had dry eye syndrome, two wore contact lenses and had dry eye syndrome, and eight were normal. Five of the eight normal eyes were scheduled to u...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.9.960.5040
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:To evaluate how patient satisfaction after cataract surgery is associated with postoperative visual acuity, visual functioning, dry eye signs and symptoms, health anxiety, and depressive symptoms. PATIENTS AND METHODS:Fifty-four patients (mean age: 68.02 years) were assessed 2 months after uneventful phacoemul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1262429
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:To quantitatively measure the flow, nonflow, density, and foveal avascular zone (FAZ) assessment tool parameters of optical coherence tomography angiography (OCTA) in patients with nonocular Behcet's Disease (BD), and to compare the results with those obtained in healthy controls. METHODS:FAZ parameters, flow ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1530361
更新日期:2019-02-01 00:00:00
abstract:PURPOSE:To compare the abilities of balanced salt solution, BSS Plus and Hartmann's lactated Ringer's (HLR) solution to maintain the viability of retinal vascular endothelial cells (RVEC) in vitro. METHODS:Cultured retinal vascular endothelial cells were suspended in each irrigating solution for four hours. Viability ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.3.239.15414
更新日期:1997-03-01 00:00:00
abstract::In the bovine lens the gamma IV-crystallin fraction is a principal determinant of the phase separation and opacification temperature, Tc (Siezen et al, Proc. Natl. Acad. Sci. USA 82, 1985, 1701). We have now measured the effect on Tc of purified gamma IV-crystallin solutions produced by a variety of reagents which aff...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509003352
更新日期:1985-10-01 00:00:00
abstract::Inhibition of ciliary process adenylate cyclase was studied on rabbit membrane preparations. When considered individually, epinephrine, GTP and NaCl did not inhibit adenylate cyclase activity. On the other hand, when present together, epinephrine, GTP (10(-5) M) and NaCl (200 mM) acted synergistically to cause a 27% i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809047034
更新日期:1988-03-01 00:00:00
abstract:PURPOSE:Pigmentation of the iris is caused by varying amounts of melanin pigment granula in a constant number of melanocytes in the superficial stroma. Melanin has been shown to act as antioxidant. We have now investigated lipid peroxidation in dependence on stromal pigmentation in isolated porcine irises. METHODS:The...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.3.229.5511
更新日期:2001-03-01 00:00:00
abstract:PURPOSE:So far tear substitutes have demonstrated a limited role in restoring ocular surface damage in dry eye syndrome (DES). The aim of this study was to assess the efficacy of a new ocular surface modulator in an in vitro model of human corneal epithelium (HCE) damaged by severe osmotic stress mirroring the features...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1184282
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:Psychiatric conditions and not just the treatments themselves might be involved in the pathophysiology of dry eye disease (DED). The aim of our study was to evaluate the association between depression and DED using objective and subjective tests in patients with newly diagnosed depressive disorder who were not ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1236966
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Endothelin-1 (ET-1) is a potent vasoconstrictive and neural peptide that has been demonstrated to be present and functionally active and important in the eye. This study was undertaken to examine for the first time the cellular distribution of ET-1 in the whole human eye. METHODS:Twelve human eyes were examine...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.5.541.5187
更新日期:1998-05-01 00:00:00
abstract:PURPOSE:The aim of this pilot study was to determine the presence of complement activation products in tears from pre- and postkeratoplasty eyes and the fellow eyes in order to investigate the activation of the classical and alternative pathways of the complement system in the early postkeratoplasty period. METHODS:Te...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.2.99.8165
更新日期:2002-02-01 00:00:00
abstract:PURPOSE:Unoprostone isopropyl is an ocular hypotensive that was originally produced as a prostaglandin F2alpha analogue and is eventually recognized as a synthetic docosanoid. The compound is recently suggested to have potent neuroprotective ability in the retina. The purpose of this study is to test whether and how th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490889465
更新日期:2004-12-01 00:00:00
abstract::Objectives: To investigate the characteristics of margin reflex distance 1 (MRD1) asymmetry in congenital lower eyelid epiblepharon and its resolution after surgical correction of epiblepharon. Methods: Among patients who underwent lower eyelid epiblepharon surgery from November 2015 to September 2017, patients with a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1857781
更新日期:2020-12-15 00:00:00
abstract::Antifibrosis agents have improved the success of glaucoma filtration surgery, although undesired side effects are not readily reversible and may present a major limitation in the use of these agents. Our purpose was to study the efficacy of cytosine arabinoside (Ara-C) as an adjunctive antimetabolite in glaucoma surge...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409025140
更新日期:1994-11-01 00:00:00
abstract::Calcium permeability was significantly increased in lenses from rats 36 h following a single injection of a cataractogenic dose of sodium selenite (30 nmol/g body weight) and this permeability returned to control values by 72 h post-injection (PI). The greater Ca2+ permeability could be partially reversed by incubatin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999466
更新日期:1993-03-01 00:00:00
abstract::Cytoskeletal and membrane proteins were isolated from respectively the urea-soluble and urea-insoluble fractions of the squid lens. The main cytoskeletal polypeptide has a molecular weight (63000) and an amino acid composition similar to those of vertebrate intermediate filament proteins, including mammalian lens vime...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688208996358
更新日期:1982-01-01 00:00:00
abstract::The effects of light damage were studied in four inbred strains of albino rats, Fischer (F344), Lewis (L), Wistar (W), and Buffalo (BUF). Thirty-five-day-old rats (3 from each strain) were kept in cyclic light for two weeks, after which they were exposed to constant fluorescent light (180-190 foot-candles, 490-580 nm)...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999576
更新日期:1990-08-01 00:00:00
abstract:PURPOSE:To investigate the morphological or functional differences of retina in amblyopia. MATERIALS AND METHODS:Forty-one patients with unilateral strabismic, anisometropic or combined amblyopia were included in the study. A control group was composed of 16 normal children. All participants were tested with optical c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.779721
更新日期:2013-07-01 00:00:00
abstract::Corneal acidosis has been shown to reduce corneal hydration control (CHC) as measured by the rate, expressed as the percent recovery per hour (PRPH), at which the thickness of the cornea decreases exponentially after an increased hydration load. Since the effect of pH on corneal function is of scientific interest and ...
journal_title:Current eye research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/02713689508999932
更新日期:1995-05-01 00:00:00