Abstract:
PURPOSE:Recent advances in technology now provide tools capable of tracking genome-wide expression changes occurring in progressive pathological processes. The present experiments were carried out to determine if acetylcholine receptor α 6 subunit (Chrna6) is a reliable retinal ganglion cell (RGC) marker in adult mouse eyes and if Chrna6 expression can be used to track progressive loss of RGCs, such as is observed in the DBA/2J glaucoma model. METHODS:Data sets derived from the BXD strains were used to extract gene expression signatures for RGCs. Pooled retinas from DBA/2J or C57BL/6J cases at 1-3 months, 12 months, and 16-17 months were prepared for gene-array and RT-PCR analysis. Globes were fixed in paraformaldehyde and sectioned for immunofluorescence with antibodies against Chrna6. RESULTS:Chrna6 has a cellular expression signature for RGCs with high correlation to Thy1 (r = 0.65), a recognized RGC marker. Immunofluorescence experiments confirm that in the young and adult mouse retina, Chrna6 is preferentially expressed by RGCs. We further show that C3H/HeJ retinas, which lack photoreceptors, also express Chrna6 in the RGC layer. Gene expression array analyses, confirmed by RT-PCR, show progressive loss of Chrna6 expression in retinas of the DBA/2J glaucomatous mouse retinas. CONCLUSIONS:Quantitative trait locus analysis provides support for Chrna6 as a RGC marker. Chrna6 expression decreases with death of RGCs in glaucomatous DBA/2J mice and after optic nerve crush injury, further supporting Chrna6 as a reliable RGC marker. High expression of RGC Chrna6 in the absence of photoreceptors is suggestive that Chrna6 expression by RGCs is independent of photoreceptor-derived stimuli.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Munguba GC,Geisert EE,Williams RW,Tapia ML,Lam DK,Bhattacharya SK,Lee RKdoi
10.3109/02713683.2012.724512subject
Has Abstractpub_date
2013-01-01 00:00:00pages
150-7issue
1eissn
0271-3683issn
1460-2202journal_volume
38pub_type
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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doi:10.3109/02713688509000845
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doi:10.3109/02713689609000760
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doi:10.1080/02713680802045099
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journal_title:Current eye research
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doi:10.3109/02713688609015138
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更新日期:2013-10-01 00:00:00
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更新日期:2011-11-01 00:00:00
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更新日期:2016-09-01 00:00:00
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