Abstract:
PURPOSE:Unoprostone isopropyl is an ocular hypotensive that was originally produced as a prostaglandin F2alpha analogue and is eventually recognized as a synthetic docosanoid. The compound is recently suggested to have potent neuroprotective ability in the retina. The purpose of this study is to test whether and how the biologically active metabolites of unoprostone isopropyl rescue retinal neuro-glial progenitor cells from apoptosis. METHODS:R28 cells were deprived of serum for 24 hr with or without varying concentrations of unoprostone metabolite M1 or M2 or vehicle in the presence or absence of specific inhibitors against several types of signal transduction proteins. Immunocytochemistry against activated caspase-3 with Hoechst nuclear staining was performed. RESULTS:Up to 15%of R28 cells became pyknotic and activated caspase-3 immunoreactive after 24-hr serum withdrawal. M1, but not M2, significantly reduced apoptotic cells in a dose-dependent fashion with a maximal effect at 100 microM (p < .0001). LY294002, the phosphatidylinositol 3-OH kinase (PI3K) inhibitor, and KT5823, the protein kinase G (PKG) inhibitor, reversed the antiapoptotic effect of M1. CONCLUSIONS:The unoprostone metabolite M1 protects retinal neuro-glial progenitor R28 cells from apoptosis induced by serum deprivation via the PI3K and PKG pathways.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Mukuno H,Nakamura M,Kanamori A,Nagai A,Negi A,Seigel Gdoi
10.1080/02713680490889465subject
Has Abstractpub_date
2004-12-01 00:00:00pages
457-64issue
6eissn
0271-3683issn
1460-2202pii
Q2105K86WDFYXM4Ljournal_volume
29pub_type
杂志文章abstract::The new HERPCHEK (Dupont, No. Billerica, MA) enzyme immunosorbent assay (EIA) was used in a double-blind clinical study for rapid and specific detection of ocular herpes simplex virus (HSV) infection. This 4-hour assay can be used to demonstrate conclusively the presence of HSV antigen without culture and thereby rapi...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713688809033194
更新日期:1988-07-01 00:00:00
abstract:PURPOSE:Due to a possible link in humans between atherosclerosis, a high-fat diet, and the development of age-related retinal degenerations, we investigated retinal changes with age and diet in high-fat-fed C57BL/6 mice, a mouse model of human atherosclerosis. METHODS:We fed C57BL/6J mice either a normal chow diet or ...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Glucocorticoid-induced ocular hypertension has been demonstrated in both animals and humans. It is possible that glucocorticoid-induced changes in trabecular meshwork (TM) cells are responsible for this hypertension. In order to elaborate further the effect of glucocorticoids on the trabecular meshwork, the ultrastruc...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309020383
更新日期:1993-09-01 00:00:00
abstract::Purpose: Dual wavelength retinal oximetry allows the quantification of oxygen saturation in the larger retinal vessels. However, the technique might be refined further by identifying factors that are responsible for the different oxygen saturations in first order arterioles from the same eye shortly after their branch...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1524015
更新日期:2019-01-01 00:00:00
abstract::Purpose/Aim: To determine the associations among socioeconomic factors, delay in management, and high-risk histopathologic features in eyes primarily enucleated for retinoblastoma. Materials and Methods: A single-site survey was conducted from January 2016 through January 2018. Eyes primarily enucleated for unilateral...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1623898
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:To facilitate studies of human retina and utilization of human retinal tissue for treatment of retinal diseases, we studied morphologic preservation in postmortem human retina. METHODS:Morphology of retinas from thirty-one human eyes was examined using light and electron microscopy. The inner and outer retina,...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Intraocular inflammation of exogenous origin, which may lead to the loss of visual function in both eyes, i.e. SO confronts ophthalmologists much less rarely than might be expected. Epidemiological studies comprising ergophthalmological aspects underscore this. Questions with regard to therapy should consider the fact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999411
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:To compare the clinical outcomes following Descemet's membrane endothelial keratoplasty (DMEK) with 100% air tamponade versus 10% sulfur hexafluoride (SF6) tamponade. METHODS:Retrospective analysis of 108 consecutive DMEK cases subdivided by anterior chamber tamponade with 54 eyes receiving 10% SF6 and 54 eyes...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1431286
更新日期:2018-04-01 00:00:00
abstract::An image analyser installed in a 16 bit personal computer was used to measure grey levels in red free photographs of normal and glaucomatous eyes. Regional photographic image intensity and density were correlated with Humphrey Field Analyser results for corresponding areas of visual field. Two methods for standardisin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033210
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:Whereas many previous studies have identified the association between sustained near work and myopia, few have assessed the influence of concomitant levels of cognitive effort. This study investigates the effect of cognitive effort on near-work induced transient myopia (NITM). METHODS:Subjects comprised of six...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.6.363.18190
更新日期:2003-12-01 00:00:00
abstract:PURPOSE/AIM:Cultured autologous oral mucosal epithelial cells (OMECs) have proven useful in the treatment of ocular surface disorders. This study is the first to investigate the potential of expanding OMEC in a xenobiotic- and serum-free medium using therapeutic contact lenses (CLs) as a substrate and carrier. MATERIA...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1004720
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Corneal scarring is a common poor outcome of corneal trauma. Transforming growth factor β-1 plays a vital role in corneal fibrosis, inducing keratocyte transformation to myofibroblasts. Other than corneal transplantation, no other curative treatment methods for corneal scarring are currently available. Hypercap...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1820526
更新日期:2020-09-11 00:00:00
abstract::It has been suggested that the provision of HLA matched tissue for corneal transplantation would be beneficial for graft survival especially in high risk patients. In this study we report the application of a polymerase chain reaction (PCR) based tissue typing procedure to type cadaveric donor material. Using beta-glo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209001798
更新日期:1992-05-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the effect of vitreous reflux (VR) on the short-term effect of intravitreal ranibizumab injection. MATERIALS AND METHODS:The study included 181 eyes of 81 age-related macular degeneration (AMD) and 100 diabetic macular edema (DME) patients. Treatment response was evaluated...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1789664
更新日期:2021-02-01 00:00:00
abstract::The administration of 0.1-0.5% of ethanol produces a slow increase in the transepithelial potential (TEP) of about 2 mV in the bovine pigment epithelium (RPE) under ordinary room lighting. However, virtually no response could be observed when ethanol was administered in the dark. Because of this apparent light sensiti...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999905
更新日期:1994-09-01 00:00:00
abstract:PURPOSE:So far tear substitutes have demonstrated a limited role in restoring ocular surface damage in dry eye syndrome (DES). The aim of this study was to assess the efficacy of a new ocular surface modulator in an in vitro model of human corneal epithelium (HCE) damaged by severe osmotic stress mirroring the features...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1184282
更新日期:2017-03-01 00:00:00
abstract::Changes in the content of calcitonin gene-related polypeptide (CGRP) and tyrosine hydroxylase (TH) immunoreactivities in the anterior segment of the rat eye were assessed histochemically in animals subjected to sympathetic ganglionectomy or to chronic sensory denervation induced by neonatal capsaicin treatment. In the...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033207
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:Nuclear factor-kappa B (NF-κB) has been proposed as a therapeutic target for the treatment of cataracts. The authors investigated the relationship between nuclear factor of activated T cells 5 (NFAT5) and NF-κB in ultraviolet B (UVB)-irradiated human lens epithelial (HLE) cells. METHODS:Human lens epithelial B...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1270327
更新日期:2017-07-01 00:00:00
abstract:PURPOSE:To determine whether environmental factors or bacterial viability affect the binding of two strains of P. aeruginosa to mouse cornea. METHODS:Scarified corneas were placed in organ culture and inoculated with P. aeruginosa cell suspensions containing either ATCC 19660 or PAO1 bacterial strains classed as cytot...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.3.231.5226
更新日期:1998-03-01 00:00:00
abstract:AIM:To test the feasibility of implanting human anterior lens capsules (HALCs) with porcine corneal endothelial cells (pCEC) in vivo in Göttingen minipigs and at the same time test the suitability of Göttingen minipig as model for endothelial keratoplasty. MATERIALS AND METHODS:Cell-carrier constructs of decellularize...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1706747
更新日期:2020-08-01 00:00:00
abstract:PURPOSE:To test the ability of two commercially available hydrogen peroxide disinfection solutions, one containing a surfactant and one without, to remove lipid from various contact lens materials using in vitro radiochemical experiments. METHODS:Etafilcon A, senofilcon A and balafilcon A contact lens materials were i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682636
更新日期:2012-09-01 00:00:00
abstract::Purpose: To evaluate the effect of methylphenidate on visual field testing in healthy adults with abnormal visual field results. Methods: This prospective, randomized, controlled interventional clinical trial comprised all patients who had abnormal visual field test results and normal eye examination and ophthalmic hi...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1858430
更新日期:2020-12-21 00:00:00
abstract::In the unsutured partial thickness penetrating wounds of the cornea, the epithelium migrates over the wounded stromal surface prior to the onset of stromal regeneration. To determine the possible affects of the epithelial ingrowth on the organization of the stromal scar tissues, the healing of unsutured and sutured wo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508995803
更新日期:1995-09-01 00:00:00
abstract:PURPOSE:To evaluate transcorneal penetration of topically applied 0.5% levofloxacin into the aqueous humor in human eyes. METHODS:Twenty cataract patients (14 females, 6 males) received 3 drops of 0.5% levofloxacin at 15 min intervals from 90 minutes before the surgery. At the beginning of the cataract surgery, 50 mic...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.5.403.8517
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:Phoria is used in the diagnosis of binocular dysfunctions, such as convergence insufficiency. A common treatment for convergence insufficiency is vision therapy, yet it is controversial whether the phoria changes as a result of rehabilitation. Phoria can vary due to the duration and/or the type of prior visual ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.694551
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:To compare the quality of corneal endothelium of precut Descemet-stripping automated endothelial keratoplasty (DSAEK) tissue when transported with and without the anterior lamellar corneal tissue (ALCT) when organ-culture corneal storage methods are used. METHODS:After microkeratome-assisted excision of anteri...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.648045
更新日期:2012-02-01 00:00:00
abstract::The intraocular inflammatory reaction in a murine model of herpes simplex keratouveitis was studied using immunohistochemical techniques. 5 x 10(5) PFU of HSV-1 (Stewart strain) was applied to the abraded cornea of BALB/c mice. The subsequent development of keratouveitis was documented by sequential examination, conve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
abstract::A model system for retinal adhesion, consisting of interacting bovine pigment, epithelium (PE) cells and photoreceptor outer segments, was utilized to examine any adhesive role of the interphotoreceptor matrix. PE cells were dissociated by trypsin treatment of the eyecup, and were allowed to replenish their surface pr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001859
更新日期:1981-01-01 00:00:00
abstract:AIM OF THE STUDY:The similarity of many benign intrabulbar lesions to a malignant tumor requires detailed differential diagnostics. However, none of the known methods can be used as the only one to determine the type of lesions. The aim of this study was to determine color Doppler imaging (CDI) markers characteristic o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1341534
更新日期:2017-11-01 00:00:00
abstract:PURPOSE:To determine the effectiveness of topically applied fluoroquinolones for experimental Pseudomonas or Serratia keratitis. METHODS:Bacteria were injected intrastromally (10(3) colony forming units [CFU]). From 16 to 22 hours post-infection (PI), a single topical drop of moxifloxacin (Vigamox, 0.545%), levofloxac...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.28.5.337.28676
更新日期:2004-05-01 00:00:00