Abstract：
PURPOSE:To facilitate studies of human retina and utilization of human retinal tissue for treatment of retinal diseases, we studied morphologic preservation in postmortem human retina. METHODS:Morphology of retinas from thirty-one human eyes was examined using light and electron microscopy. The inner and outer retina, rod and cone photoreceptor cells, and central and peripheral retina were compared with regard to morphologic preservation. Possible factors affecting survival were analyzed. RESULTS:The earliest postmortem change was vacuolation of the nerve fiber layer within a few hours postmortem, followed by vacuolation and cytoplasmic swelling of the inner retina. As compared with the inner retina, outer retinal structure was better preserved, i.e., the photoreceptor cells maintained better morphology. Rod cell morphology was better preserved than cone cell morphology, with good preservation of the rod outer segment disc membranes and the inner segment mitochondrial membranes. Thus, well-preserved rod photoreceptor cells were evident in specimens at least 48-hours postmortem. Peripheral retina was better preserved than the central retina including the fovea and perifovea. Factors affecting anatomical integrity included the total time postmortem and, more importantly, the time between death and enucleation. Other factors, including age and sex, did not appear to affect morphological preservation in the present study. CONCLUSIONS:Human retina postmortem remained morphologically intact for a relatively long period of time, with differential preservation among different geographic areas and cell types. This morphologic evidence is consistent with previous findings of functional preservation (e.g. , photoresponses) in such tissue. This study may shed some light on understanding of human retina and its utilization for retinal transplantation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Huang JC,Voaden MJ,Zarbin MA,Marshall Jsubject
Has Abstractpub_date
2000-03-01 00:00:00pages
231-41issue
3eissn
0271-3683issn
1460-2202journal_volume
20pub_type
杂志文章abstract：:Adult albino mice received tritiated thymidine either injected subcutaneously (333 microCi) every 6 hours for up to 36 hours, or administered in their drinking water for up to 74 days (about 45 microCi/day). Tissue sections were examined with autoradiography to determine the distribution of the label. Animals receivin...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688809047029
更新日期：1988-03-01 00:00:00
abstract：PURPOSE:To determine the effects of a subconjunctival injection of mitomycin-C (MMC) on iridial circulation in rabbits. METHODS:Dutch rabbits anesthetized with pentobarbital received a 0.2-ml subconjunctival injection of 0.4 mg/ml or 0.1 mg/ml MMC; the contralateral eye received 0.2 ml physiological saline. Intraocula...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1076/ceyr.19.2.197.5327
更新日期：1999-08-01 00:00:00
abstract：OBJECTIVE:Children with chronic kidney diseases (CKD) are at risk for neurological diseases at early adulthood. Spectral-domain optical coherence tomography (SD-OCT) of the retina is especially suitable for determination of intraretinal layer thickness. We wonder whether retinal thinning is already present in pediatric...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2018.1522649
更新日期：2019-02-01 00:00:00
abstract：:3-aminotriazole (3AT) is known to reduce catalase levels in ocular tissues when given intravenously or orally. Rabbits were given either 4 ml/kg of a 3M solution of 3AT intravenously or a 2% solution as drinking fluid. Intravenous 3AT administration was followed at 4 hrs by an intracameral injection of hydrogen peroxi...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688709044504
更新日期：1987-12-01 00:00:00
abstract：PURPOSE:To explore the in vitro proliferative potential of human limbal epithelial cells after 31 degrees C organ-culture storage and to investigate putative factors influencing it. METHODS:185 cultures of limbal explants were carried-out either from full-thickness explants (n = 102) or from enzymatically dissociated ...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713680600681228
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:We report on the use of subconjunctival bevacizumab on corneal vessel density in recurrent pterygia. METHODS:The charts of 5 patients with recurrent pterygium, who received subconjunctival injections of bevacizumab (2.5 mg/0.1 ml) were retrospectively reviewed. Ophthalmic evaluation included Snellen visual acu...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713680701799101
更新日期：2008-01-01 00:00:00
abstract：AIM OF THE STUDY:The similarity of many benign intrabulbar lesions to a malignant tumor requires detailed differential diagnostics. However, none of the known methods can be used as the only one to determine the type of lesions. The aim of this study was to determine color Doppler imaging (CDI) markers characteristic o...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2017.1341534
更新日期：2017-11-01 00:00:00
abstract：PURPOSE:The purpose of this work is to describe and review the technology of plasma rich in growth factors (PRGF), a novel blood derivative product, in the treatment of ocular surface disorders. METHODS:To demonstrate the importance of this technology in the treatment of ocular pathologies, a thorough review of the pr...
journal_title：Current eye research
pub_type： 杂志文章,评审
doi：10.3109/02713683.2015.1104362
更新日期：2016-07-01 00:00:00
abstract：:Purpose: To evaluate the microvascular density of the peripapillary and parafoveal areas of the eyes with primary angle closure disease (PACD) using optical coherence tomography angiography (OCTA). Materials and methods: An observational study was conducted with 76 PACD patients (29 eyes with primary angle closure sus...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2020.1849728
更新日期：2020-11-26 00:00:00
abstract：PURPOSE:To evaluate indocyanine green angiography (ICGA) for the identification and characterization of afferent (feeding) and efferent (draining) vessels in patients with ocular surface neoplasia. MATERIALS AND METHODS:Consecutive patients with biopsy-proven benign, pre-invasive, or invasive ocular surface tumors of ...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2017.1387273
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2016.1250278
更新日期：2017-05-01 00:00:00
abstract：:Due to the multiple barriers imposed by the eye against the penetration of drugs, the ocular delivery and targeting are considered difficult to achieve. A major challenge in ocular drug therapy is to improve the poor bioavailability of topically applied ophthalmic drugs by overcoming the severe constraints imposed by ...
journal_title：Current eye research
pub_type： 杂志文章,评审
doi：10.3109/02713681003760168
更新日期：2010-07-01 00:00:00
abstract：PURPOSE:Intrastromal epithelial migration occurs following deep excimer annular keratectomy. Our purpose is to determine the localization of gelatinase B expression and its relationship to migrating intrastromal epithelial cells following deep annular excimer keratectomy. METHODS:Rabbit corneas were treated with deep ...
journal_title：Current eye research
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:In an attempt to determine the factors involved in ocular pigment variation, the eyes of pallid mice (c57BL/6J-Pa/Pa), and those of littermate black mice (C57BL/6J-+/Pa) were examined ultrastructurally, histochemically, and biochemically. As controls, the eyes of black and congenic albino mice (C57BL/6J +/C2J and -C2J...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688208998376
更新日期：1982-01-01 00:00:00
abstract：:The corneal endothelium of alloxan diabetic dogs was examined by wide-field specular microscopy and compared to that of age-matched non-diabetic dogs. Computer-assisted morphometry of individual cells showed that the diabetic dog endothelium had marked polymegethism and pleomorphism similar to that previously describe...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688509020031
更新日期：1985-07-01 00:00:00
abstract：:DNA sequence analysis of overlapping shotgun and restriction fragments have revealed the entire sequence of the human lens fiber cell intrinsic membrane protein MP19 gene (also termed MP17, MP18, and MP20). The 8,056 bp MP19 gene contains 5 exons encoding a mature protein of 173 amino acids, which displayed a very hig...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713689309033503
更新日期：1993-12-01 00:00:00
abstract：:Intravitreal injection of purified human chorionic gonadotropin (hCG) in rabbits decreased intraocular pressure (IOP). A dose-dependent decrease in IOP was observed with intravitreal hCG concentrations at 30 nM and 100 nM. The onset of this effect was later than 10 hr following the injection and it lasted for more tha...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688809015151
更新日期：1988-10-01 00:00:00
abstract：:Purpose: To determine the refractive astigmatism following toric intraocular lens (tIOL) implantation without consideration of posterior keratometric astigmatism with a conventional tIOL calculator for eyes with low keratometric astigmatism (0.75D to 2.5D) and to theoretically compare the outcomes with predicted refra...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2019.1638418
更新日期：2019-12-01 00:00:00
abstract：UNLABELLED:Purpose. To develop a rapid and accurate method for the quantification of reduced glutathione (GSH) and oxidized glutathione (GSSG) using micro-quantities of ocular lens. Methods. The epithelium, cortex and nucleus of the lens were separated and also the whole lens was homogenized in 3% metaphosphoric acid. ...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713689609003455
更新日期：1996-07-01 00:00:00
abstract：PURPOSE:To examine the effects of external modulation of epithelial-mesenchymal interaction on conjunctival epithelial cell differentiation characteristics. METHODS:Keratin K4 and laminin beta2 chain protein localization was examined in an organotypic model which facilitates the comparison of differentiation character...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1076/ceyr.18.1.28.5394
更新日期：1999-01-01 00:00:00
abstract：PURPOSE:To investigate the morphological or functional differences of retina in amblyopia. MATERIALS AND METHODS:Forty-one patients with unilateral strabismic, anisometropic or combined amblyopia were included in the study. A control group was composed of 16 normal children. All participants were tested with optical c...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713683.2013.779721
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:Oxidative damage of lens tissue contributes to the formation of age-related cataract. Pi-class glutathione-S-transferase (GSTP1) plays a role in the removal of oxidative adducts by transferring them to glutathione. To assess epigenetic regulation of GSTP1 and its potential role in age-related nuclear cataract (...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2016.1185129
更新日期：2017-03-01 00:00:00
abstract：:Water-soluble (WS) and water-insoluble (WI) crystallins were prepared from calf and bovine lenses. The WS-crystallins were analyzed by capillary isotachophoresis and by immunoelectrophoresis with polyvalent and alpha-, beta-, gamma-crystallin specific antisera. The WI-crystallins were first solubilized in formamide an...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688409065590
更新日期：1984-05-01 00:00:00
abstract：PURPOSE:Nona-D-arginine amide (D9R) affected a cure of Pseudomonas aeruginosa corneal disease when combined with ciprofloxacin. In this study, we show that D9R alone and prophylactic treatment with D9R significantly reduced corneal pathology and bacterial burden associated with P. aeruginosa infection. MATERIALS AND M...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713680903487609
更新日期：2010-03-01 00:00:00
abstract：PURPOSE:To evaluate the length of the trabecular meshwork (TM) from the scleral spur (SS) to Schwalbe's line (SL) and assess the detectability of the SS in histopathology specimens. METHODS:This study included 158 angle images from 79 cross-sectional slides derived from eyes enucleated for melanoma. The slides were st...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713683.2012.700757
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:To investigate the repeatability of macular and peripapillary retinal nerve fiber layer (RNFL) thickness measurements made using swept-source optical coherence tomography (SS-OCT) and automated segmentation. Measurements were made in non-diabetic controls and in patients with diabetic retinopathy (DR) with or w...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713683.2016.1170855
更新日期：2017-02-01 00:00:00
abstract：:The present study examined the anti-herpetic effect of the glycoprotein inhibitors, hydroxynorvaline and 2-deoxyglucose, alone and in combination with trifluridine on murine ocular herpes. Following ocular inoculation with a large dose of HSV-1 RE strain (10(6) pfu), ICR mice were treated during the acute infection wi...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713688609015097
更新日期：1986-02-01 00:00:00
abstract：:Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newb...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.3109/02713689608995831
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:To determine the role of apoptosis in the pathogenesis of cytomegalovirus retinitis in patients with the acquired immunodeficiency syndrome. METHODS:Forty-three eyes from patients with cytomegalovirus retinitis treated before the introduction of highly active anti-retroviral therapy were examined by routine hi...
journal_title：Current eye research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：PURPOSE:Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular developm...
journal_title：Current eye research
pub_type： 杂志文章
doi：10.1080/02713683.2018.1545911
更新日期：2019-04-01 00:00:00
