Abstract:
PURPOSE:To explore the in vitro proliferative potential of human limbal epithelial cells after 31 degrees C organ-culture storage and to investigate putative factors influencing it. METHODS:185 cultures of limbal explants were carried-out either from full-thickness explants (n = 102) or from enzymatically dissociated cells (n = 83) seeded on a feeder layer of human keratocytes. Epithelial outgrowth was assessed by phase contrast microscopy using a computerized image analysis software. Cell phenotype was evaluated by transmission electron microscopy and immunocytology. Univariate and multivariate analysis were performed to determine factors influencing epithelial growth in culture. RESULTS:An epithelial outgrowth of 100 square mm or more was observed in 52% of cultures, (average growth area: 440 +/- 256 mm at three weeks). Corneal epithelial phenotype was confirmed by transmission electron microscopy, and cytokeratin pattern. Cytokeratine 19, deltaNp63, nestin and vimentin positive staining revealed undifferentiated epithelial cells in both explant and cell suspension cultures at three weeks. Short death to cornea retrieval time (p < 0.03) and female donors (p < 0.01) were associated with higher cell growth. Enzymatic treatment of explants by trypsin, but not dispase, decreased cell proliferation at two (p < 0.03) and three weeks (p < 0.04). Donor age, duration of corneal storage, and source of the explant did not influence the cell growth. CONCLUSION:Organ-culture conditions can preserve limbal cell mitotic potential if limbal tissue is excised early after circulatory arrest. Human keratocytes can be used as a feeder layer allowing epithelial cells to maintain poorly differentiated phenotype in culture. Further investigations are needed to explain the influence of the donor sex on epithelial cell growth in culture.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Zito-Abbad E,Borderie VM,Baudrimont M,Bourcier T,Laroche L,Chapel C,Uzel JLdoi
10.1080/02713680600681228subject
Has Abstractpub_date
2006-05-01 00:00:00pages
391-9issue
5eissn
0271-3683issn
1460-2202pii
U07109X7W0531243journal_volume
31pub_type
杂志文章abstract:PURPOSE:The aim of this pilot study was to determine the presence of complement activation products in tears from pre- and postkeratoplasty eyes and the fellow eyes in order to investigate the activation of the classical and alternative pathways of the complement system in the early postkeratoplasty period. METHODS:Te...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.2.99.8165
更新日期:2002-02-01 00:00:00
abstract:PURPOSE:Previous studies showed that lutein and zeaxanthin, the major human retinal carotenoids, are concentrated in the macula. In this study, the carotenoids in human macular and peripheral retina and the retinal pigment epithelium (RPE) were analyzed. They were also determined in the rod outer segments (ROS) before ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.6.491.5276
更新日期:1999-12-01 00:00:00
abstract::The maximal velocity (Vmax) and the apparent dissociation constant (K0.5) of Na+K+-ATPase have each been estimated with respect to sodium and potassium ion activation. These estimations were made from the enzymatic activity of plasma membrane preparations derived from bovine corneal endothelial cells. The determinatio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034834
更新日期:1987-05-01 00:00:00
abstract:PURPOSE:It has been demonstrated that chick eye growth compensates for defocus imposed by spectacle lenses: the eye elongates in response to hyperopic defocus imposed by negative lenses and slows its elongation in response to myopic defocus imposed by positive lenses. We ask whether the synthesis of scleral extracellul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.4.320.10697
更新日期:1997-04-01 00:00:00
abstract:PURPOSE/AIM OF THE STUDY:Polarization-sensitive anterior-segmental optical coherence tomography (PS-OCT) is useful to evaluate the filtering blebs after trabeculectomy. However, the attenuation phenomenon of OCT signal disturbs the visibility of intra-bleb's structure and distribution of birefringence; a specific param...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1825749
更新日期:2020-10-04 00:00:00
abstract::We established growth curves for Propionibacterium acnes isolates recovered from eyes with chronic postoperative endophthalmitis. The growth curve plotted the average of the duplicate bacterial concentration against time. The generation time for P. acnes calculated from the growth curves was approximately 5.1 hours. T...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999875
更新日期:1994-06-01 00:00:00
abstract::Steroids are widely used in the prevention of corneal neovascularization in a wide range of natural and experimental situations. However, no information is available on their effect on the growth of the individual limbal blood vascular cells or of lymphatic cells involved in corneal neovascularization. In addition, tr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508999908
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:Accumulation of lipoprotein-derived lipids including esterified and unesterified cholesterol in Bruch's membrane of human eyes is a major age-related change involved in initiating and sustaining soft drusen in age-related macular degeneration (AMD). The apolipoprotein (apo) A-I mimetic peptide 4F is a small ant...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1370118
更新日期:2018-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.971937
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To determine whether the usual clinical dose of intravitreal dexamethasone can attenuate intraocular inflammation and retinal necrosis in a rabbit model of fulminant Bacillus cereus endophthalmitis induced by crude exotoxins. METHODS:Thirty-six eyes from pigmented mongrel rabbits received intravitreal injectio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490516701
更新日期:2004-10-01 00:00:00
abstract:PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903509410
更新日期:2010-04-01 00:00:00
abstract:AIMS:To elucidate the intramuscular distribution and branching patterns of the abducens nerve in the lateral rectus (LR) muscle so as to provide anatomical confirmation of the presence of compartmentalization, including for use in clinical applications such as botulinum toxin injections. METHODS:Thirty whole-mount hum...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1438631
更新日期:2018-06-01 00:00:00
abstract:PURPOSE:Intravitreal injection of ciliary neurotrophic factor (CNTF) is known to induce glial intermediate filament protein (GFAP) expression in retinal Müller cells. Because CNTF binding can activate multiple signaling kinases, we have examined the involvement of JAK/STAT pathway in GFAP induction in Müller cells. ME...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.4.305.8408
更新日期:2002-04-01 00:00:00
abstract:PURPOSE:To evaluate the immediate effect of 3% diquafosol ophthalmic solution on tear MUC5AC concentration, periodic acid-Schiff (PAS)-positive goblet cells, and tear film stability in normal and keratoconjunctivitis sicca (KCS) rat models. METHODS:Rats were divided into normal and KCS groups. 3% of diquafosol solutio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1233986
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Dry eye is a common condition known to have many systemic co-morbidities. We aim to report the frequency of depression and anxiety suspects in eye clinic patients and explore the association between dry eye signs and symptoms with depression and anxiety. MATERIALS AND METHODS:Adult outpatients were recruited f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1056804
更新日期:2016-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.2.69.10156
更新日期:2002-08-01 00:00:00
abstract::The effects of repeated exposures to UV-A (335 nm) and UV-B (305 nm) radiation on the crystalline lens were studied by treating cultured bovine lenses daily or weekly. The effects of irradiation on lens optical quality were monitored using an automated scanning laser system that records both relative transmittance and...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409167301
更新日期:1994-05-01 00:00:00
abstract::We measured the relative solubility of collagen in acetic acid after pepsin digestion and tentatively identified the types of collagen present in corneas of rabbits of various ages and in corneal scar tissue, using hydroxyproline assays and polyacrylamide gel electrophoretic analyses. More than 80% of the collagen in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019966
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To study whether there exists a relationship between retinal nerve fiber layer thickness and oxygen saturation and to identify whether perivascular thickness (pRNFL) or quadrant thickness (qRNFL) has a stronger correlation. METHODS:Patients without any detectable ocular abnormality on complete ophthalmic evalu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1104361
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To determine the role of matrix metalloproteinase 9 (MMP9) in advanced glycation end-products (AGEs) induced damage to blood-retinal barrier (BRB) and the therapeutic effects of minocycline, an MMP inhibitor, against the BRB damages. METHODS:Forty-eight Sprague Dawley rats were randomly assigned to 3 groups (1...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802450984
更新日期:2008-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.514658
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To examine the morphological changes in the meibomian glands of eyes of patients with dry eye disease using the non-contact infrared meibography system and to assess their relationship with meibomian dropout, signs, and tear-film function. METHODS:Subjects included 264 randomly selected patients (528 eyes) suf...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.891748
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514055
更新日期:2018-12-01 00:00:00
abstract:AIM OF THE STUDY:The similarity of many benign intrabulbar lesions to a malignant tumor requires detailed differential diagnostics. However, none of the known methods can be used as the only one to determine the type of lesions. The aim of this study was to determine color Doppler imaging (CDI) markers characteristic o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1341534
更新日期:2017-11-01 00:00:00
abstract::Spontaneous ocular shedding and adrenergic induction of ocular shedding were examined in rabbits infected with ten strains of herpes simplex virus type 1 (HSV-1): McKrae, KOS, F, Rodanus, 17 Syn+, RE, E-43, SC-16, MacIntyre, and CGA-3. All ocular inoculations were with 50 microliter of HSV-1 with titers between 1-10 X...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709020074
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To test if nicotine counteracts the dampening effect of human cytomegalovirus (HCMV) infection of NF-kappaB in retinal pigment epithelial (RPE) cells, thereby increasing the permissiveness of RPE cells for HCMV replication. METHODS:Human ARPE-19 cells were transfected with NF-kappaB luciferase DNA, inoculated ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500514651
更新日期:2006-02-01 00:00:00
abstract::Utilizing a rapid colorimetric assay that relates cell number to cytoplasmic hexosaminidase activity, we conducted drug-induced cytotoxicity experiments on human ocular fibroblasts cultured from Tenon's capsule specimens. The effects of two different agents--dimethyl sulfoxide (DMSO) or mitomycin C--on the proliferati...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999599
更新日期:1990-06-01 00:00:00
abstract::Purpose: The purpose of this study is to present the study design and one month's preliminary results of a 2-year randomized trial, Combined Atropine with Orthokeratology (AOK), for myopia control study. Methods: Children aged 6 to <11 years and with 1.00-4.00 D myopia were randomly assigned to AOK group or ortho-k al...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2019.1568501
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To quantitatively measure the flow, nonflow, density, and foveal avascular zone (FAZ) assessment tool parameters of optical coherence tomography angiography (OCTA) in patients with nonocular Behcet's Disease (BD), and to compare the results with those obtained in healthy controls. METHODS:FAZ parameters, flow ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1530361
更新日期:2019-02-01 00:00:00
abstract::Reduced glutathione (0.3 mM) stimulates the activity of sodium-potassium activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione, however, has no effect on Na+K+ATPase activity in the same tissue, although it does inhibit magnesium activated ATPase...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019985
更新日期:1981-01-01 00:00:00