Abstract:
PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved in the modulation of retinal signalling. Therefore, we tested the effect of both, ZnCl(2) (10 to 500 microM) and DEDTC (100 to 500 microM), which chelates zinc ions for the capacity to influence the ERG b-wave amplitude. METHODS:Transretinal potentials from the isolated bovine retina were recorded as electroretinograms and Ca(2+) inward currents by patch-clamp recordings of stably Ca(v)2.3 transfected HEK-293 cells, yielding an IC(50) value of 5.3 microM for ZnCl(2). RESULTS:ZnCl(2) (10-15 microM) increased the b-wave amplitude by 1.52-fold +/- 0.12 (n = 6 retinas), which was partially reversible upon washout. The same 1.5-fold stimulation of the b-wave amplitude was reported recently for 15 microM NiCl(2). The superfusion of isolated retinas by DEDTC (100 microM) caused a transient decrease of the ERG b-wave amplitude (0.75-fold +/- 0.06; n = 4), suggesting that the co-secretion of Zn(2+) ions may occur under scotopic conditions. CONCLUSION:The stimulatory effect of ZnCl(2) on the ERG b-wave amplitude resembles the stimulatory effect of NiCl(2) and may be mediated rather by the NiCl(2)-sensitive, Ca(v)2.3 E-/R-type voltage-gated Ca(2+) channels than by NiCl(2)-sensitive T-type channels.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Siapich SA,Wrubel H,Albanna W,Alnawaiseh M,Hescheler J,Weiergräber M,Lüke M,Schneider Tdoi
10.3109/02713680903509410subject
Has Abstractpub_date
2010-04-01 00:00:00pages
322-34issue
4eissn
0271-3683issn
1460-2202journal_volume
35pub_type
杂志文章abstract::The corneal endothelium is known to contain gap junctions on its lateral borders. Here we show that the endothelial cells of the rabbit cornea are highly dye coupled. Lucifer Yellow CH introduced into a single cell through either an intracellular glass microelectrode or a patch electrode rapidly spreads into its neigh...
journal_title:Current eye research
pub_type: 杂志文章
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journal_title:Current eye research
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