Abstract:
PURPOSE:To study the relationship of oxygen level and glucose concentration on the secretion of vascular endothelial growth factor (VEGF) mRNA and protein in several types of cultured retinal cells. MATERIALS AND METHODS:Several types of human and bovine retinal cells were cultured in medium without glucose, or containing 5 mM or 25 mM D-glucose or 5 mM D-glucose and 20 mM D-galactose. Cells were cultured in 20% O(2) ("normoxia") or in 1% O(2) ("hypoxia"). After being cultured for 8-96 hr, we measured VEGF protein in the medium and VEGF mRNA in the cell layer, as well as the concentrations of glucose, lactate, and pyruvate in the medium. RESULTS:Hypoxia increased VEGF mRNA and protein in these cells. In normoxia, culture in high glucose medium had no significant effect on basal VEGF production in normal glucose. However, culture in hypoxia and high glucose significantly blunted hypoxic VEGF up-regulation. Culture in normoxia, with no glucose in the medium, significantly increased VEGF. Culture in high galactose medium did not significantly affect VEGF production. Despite considerable lactate production, especially in the presence of 25 mM glucose, addition of strong buffers to the medium had little effect on VEGF production. CONCLUSIONS:Cultured retinal cells up-regulate their VEGF production when their energy supply, including glucose and/or O(2), is inadequate. Supplying glucose to the cells in the presence of low O(2) reduces their VEGF production. We suggest that "early worsening" of retinopathy results when diabetic patients with minimal to moderate retinopathy, whose retinal circulation and, hence, retinal oxygen supply is compromised, are placed on a "tight" glucose control regimen and their major remaining retinal energy source is reduced, with VEGF up-regulation as a compensatory mechanism.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Kennedy A,Frank RNdoi
10.3109/02713683.2010.521968subject
Has Abstractpub_date
2011-02-01 00:00:00pages
168-77issue
2eissn
0271-3683issn
1460-2202journal_volume
36pub_type
杂志文章abstract:PURPOSE:To detect the immunohistochemical changes in the main lacrimal glands of patients suffering from chronic ocular sequelae of Stevens-Johnson syndrome (SJS). METHODS:Histological sections of biopsies from the lacrimal gland of three chronic SJS patients (mean age, 33 years; 2 males) with severe dry eye disease (...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1836227
更新日期:2020-10-18 00:00:00
abstract:PURPOSE:Retinal pigment epithelial (RPE) cell proliferation is an important step in the pathogenesis of ocular diseases such as proliferative retinopathy. Growth factors play a major role in modulating the development and progression of these diseases. The aim of this study was to examine the effects of single growth f...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:To investigate the therapeutic role of topical and oral fluconazole treatment using a rabbit model of Aspergillus fumigatus keratitis. METHODS:Aspergillus fumigatus spores were injected into the corneal stroma of the right eye of 20 rabbits. Forty-eight hours later the rabbits were randomly divided into three ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.2.113.14514
更新日期:2003-02-01 00:00:00
abstract:PURPOSE:To assess the distribution of dark-light changes in iris features and associated factors amongst American Caucasians, American Chinese and mainland Chinese aged 40 years and over. METHODS:Four gender- and age-matched cohorts: Caucasians, American Chinese, Southern Chinese and Northern Chinese comprised the stu...
journal_title:Current eye research
pub_type: 杂志文章,多中心研究
doi:10.3109/02713683.2012.660593
更新日期:2012-07-01 00:00:00
abstract:PURPOSE:Choroidal neovascularization (CNV) is a major cause of vision loss in patients with age-related macular degeneration (AMD). Stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) plays a critical role in homing of bone marrow-derived cells (BMCs) to choroidal neovascularization (CNV). In this st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.593107
更新日期:2011-09-01 00:00:00
abstract:PURPOSE:Form-deprivation myopia (FDM) is believed to result principally from actions of substances that modulate information processing in the retina. We used a chick model to investigate what role nitric oxide (NO), a gaseous neuromodulator, might play in the development of FDM. METHODS:We injected different concentr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.10.992.9021
更新日期:1997-10-01 00:00:00
abstract::A prototype automated flicker perimeter was used to investigate flicker luminance thresholds in glaucoma patients (n = 11), ocular hypertensives (high risk n = 10, low and intermediate risk n = 21) and normal controls (n = 10). Differential light sensitivity was measured for one eye of each subject using a flickering ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409047005
更新日期:1994-10-01 00:00:00
abstract::Components of the Renin Angiotensin System (RAS) have been detected in ocular tissues and fluids. The source of the ocular RAS proteins is unknown but possibilities include diffusion or leakage from the systemic circulation, specific uptake from the blood, or local synthesis. We have used RT-PCR and in situ hybridizat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409047011
更新日期:1994-10-01 00:00:00
abstract::The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003066
更新日期:1984-04-01 00:00:00
abstract:OBJECTIVE:Children with chronic kidney diseases (CKD) are at risk for neurological diseases at early adulthood. Spectral-domain optical coherence tomography (SD-OCT) of the retina is especially suitable for determination of intraretinal layer thickness. We wonder whether retinal thinning is already present in pediatric...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1522649
更新日期:2019-02-01 00:00:00
abstract::The effect of cytochalasin D (CD), an actin monomer-stabilizer, has been studied on cataract development in rat lenses. Cataractogenesis was induced by incubating the rat lenses in medium 199 (M199) containing 10(-5) M CD; by the end of 24 h, lenses first developed a visible opacity. The increased lactate dehydrogenas...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508995134
更新日期:1995-10-01 00:00:00
abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001869
更新日期:1981-01-01 00:00:00
abstract::We studied the protective effects of passive immunization with virus specific antibody in newborn rabbits inoculated subcutaneously with type 2 herpes simplex virus (HSV-2). Newborn rabbits given anti-HSV-2 antibody intraperitoneally (IP) on days 0, 2 and 4 post infection had smaller herpetic skin lesions and reduced ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709020084
更新日期:1987-01-01 00:00:00
abstract::Change of urate and ascorbate levels in retinae of rat left eyes after ophthalmic artery and optic nerve ligation was studied by reversed-phase high-performance liquid chromatography with electrochemical detection. The right eyes were used for sham operation. We found that uric acid levels in the retinae of the left e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608996369
更新日期:1986-07-01 00:00:00
abstract:PURPOSE:Retinoblastoma (RB) is the most common intraocular malignancy in children. Deregulation of several miRNAs has been identified in RB, suggesting a potential role in tumorigenesis. Recent evidence suggests that many dietary components like folate, retinoids and curcumin act as potential anticancer/antiproliferati...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.647224
更新日期:2012-05-01 00:00:00
abstract::The possible roles of monocytes and lymphocytes in vitreous fibrosis were examined by injecting various numbers of the peripheral cell types into the rabbit vitreous. Our results indicated that the degree of vitreous fibrosis and the presence of traction retinal detachment corresponded to the number of cells injected....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033223
更新日期:1988-12-01 00:00:00
abstract::Lenses from normal Wistar rats and those from a Wistar strain with X-ray-induced cataract mutation were examined electron microscopically for morphological characterization and acid phosphatase (AcPase) localization. Ocular anlages of 11 through 18 days of gestation were included in the study. A late-separating or per...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003064
更新日期:1984-04-01 00:00:00
abstract::Lipid synthesis was investigated in bovine lens in organ culture for 9 hours using 14C acetate as a precursor. The greatest incorporation occurred in phospholipid fraction with phosphatidyl choline being the major phospholipid labeled. Considerable activity was incorporated in the free fatty acid fraction, cholesterol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034889
更新日期:1987-09-01 00:00:00
abstract::Purpose/Aim: To determine the associations among socioeconomic factors, delay in management, and high-risk histopathologic features in eyes primarily enucleated for retinoblastoma. Materials and Methods: A single-site survey was conducted from January 2016 through January 2018. Eyes primarily enucleated for unilateral...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1623898
更新日期:2019-10-01 00:00:00
abstract:UNLABELLED:Abstract Purpose of the Study: Protein tyrosine phosphatase σ (PTPσ) acts as a neuronal receptor for chondroitin sulfate proteoglycans (CSPGs). CSPGs have inhibitory effects on experience-dependent plasticity and usually form lattice-like cell coatings that surround the parvalbumin (PV) interneurons in the v...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.803287
更新日期:2013-10-01 00:00:00
abstract:UNLABELLED:Purpose. To develop a rapid and accurate method for the quantification of reduced glutathione (GSH) and oxidized glutathione (GSSG) using micro-quantities of ocular lens. Methods. The epithelium, cortex and nucleus of the lens were separated and also the whole lens was homogenized in 3% metaphosphoric acid. ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609003455
更新日期:1996-07-01 00:00:00
abstract::We have examined the T cell specificity of a Lewis rat T cell line (R208) specific for a pathogenic, 123 residue cyanogen bromide produced peptide of bovine S-antigen by using two independent sets of overlapping synthetic peptides representing the entire length of the 123 residue fragment. S-antigen, a 48 kDa immunopa...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999429
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:To evaluate central corneal thickness (CCT) and corneal volume (CV) in healthy eyes using swept source anterior segment optical coherence tomography (SS-AS-OCT) and other devices. METHODS:Thirty-three healthy right eyes of 33 subjects were investigated. CCT was measured with SS-AS-OCT, rotating Scheimpflug cam...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713683.2012.745878
更新日期:2013-05-01 00:00:00
abstract::Activity of RNA polymerases I, II and III (distinguished using alpha-amanitin) and activity of DNA polymerases alpha and beta (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carba...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709025204
更新日期:1987-03-01 00:00:00
abstract:PURPOSE:To study changes in the morphometric characteristics of the whole brain visual-related cortex in various stages of primary open angle glaucoma (POAG) in vivo. MATERIALS AND METHODS:Thirty POAG patients (nine early stage cases and 21 advanced-late stage cases) and 30 gender-, education-, and age-matched healthy...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.683506
更新日期:2012-09-01 00:00:00
abstract::Approximately 90% of all ophthalmic drug formulations are now applied as eyedrops. Although eyedrops are convenient and well accepted by patients, about 95% of the drug contained in the drops is lost due to absorption through the conjunctiva or through the tear drainage. A major fraction of the drug eventually enters ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500346633
更新日期:2005-12-01 00:00:00
abstract::The morphology and postnatal ontogeny of the major pathway for aqueous outflow in the canine eye has been examined. Normal canine chamber angles of the Beagle at one day, one through ten weeks, three, four and six months, and one and two years of age were used. Angle tissue was prepared and examined by standard techni...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000791
更新日期:1984-06-01 00:00:00
abstract:PURPOSE:Several procedures in cataract surgery carry the risk of high temperature increases in the capsular bag. The present study was undertaken to determine the shrinkage temperature of the human lens capsule and to investigate the effect of temperature on the mechanical behavior of the lens capsule. METHOD:Thermal-...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.5.470.5198
更新日期:1998-05-01 00:00:00
abstract::The clinical impression that pre-existing diabetes exacerbates radiation injury to the retinal vasculature was studied in STZ diabetic rats. Half of 2 groups of streptozotocin (STZ)-induced diabetic rats and 1 group of normal animals had their right eyes irradiated with 1000 cGy of 90 KVP x-rays. The prevalence of ace...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309029227
更新日期:1993-11-01 00:00:00
abstract:PURPOSE:To optimize cultured human corneal endothelial cell (cHCEC) sheet transplantation technique for maintenance of cHCEC viability. MATERIALS AND METHODS:cHCEC sheets cultured on a collagen scaffold were covered with or without Viscoat® and exposed to humidified air in the incubator. cHCEC sheets with or without V...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1101774
更新日期:2016-09-01 00:00:00