Abstract:
:Activity of RNA polymerases I, II and III (distinguished using alpha-amanitin) and activity of DNA polymerases alpha and beta (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carbamylcholine-treated and control eyes of rabbits with resurfacing acid burn defects. Incorporation was linear with time for all enzymes up to 30 min. In 10 min assays at varying substrate concentrations, all polymerases from carbamylcholine-treated eyes had significantly elevated Vmax compared to corresponding control enzymes. The drug also increased apparent affinity of RNA polymerase II for UTP and apparent affinity of DNA polymerases alpha and beta for dTTP. Results are discussed in relation to potential mechanisms by which effects of carbamylcholine on polymerase activity may be mediated.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Colley AM,Law ML,Drake LA,Cavanagh HDdoi
10.3109/02713688709025204subject
Has Abstractpub_date
1987-03-01 00:00:00pages
477-87issue
3eissn
0271-3683issn
1460-2202journal_volume
6pub_type
杂志文章abstract::The cytoskeletal proteins of the human lens were studied by SDS-PAGE, 2-D electrophoresis and immunologically. Spectrin, vimentin and actin were identified in the superficial fiber cells of human lenses even to age 87 years. These proteins are lost from the deeper cortical and nuclear fiber cells, where a broad zone o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688408997222
更新日期:1984-02-01 00:00:00
abstract::We measured the relative solubility of collagen in acetic acid after pepsin digestion and tentatively identified the types of collagen present in corneas of rabbits of various ages and in corneal scar tissue, using hydroxyproline assays and polyacrylamide gel electrophoretic analyses. More than 80% of the collagen in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019966
更新日期:1981-01-01 00:00:00
abstract::The intraocular inflammatory reaction in a murine model of herpes simplex keratouveitis was studied using immunohistochemical techniques. 5 x 10(5) PFU of HSV-1 (Stewart strain) was applied to the abraded cornea of BALB/c mice. The subsequent development of keratouveitis was documented by sequential examination, conve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
abstract::Two anti-fibrotic agents were evaluated in vitro for potential application in trabeculectomy. The rate of collagen formation by cultured rabbit corneal fibroblasts was determined by [3H]proline uptake and hydroxylation assay. Incubation of fibroblasts, for 96 hours, with 16 mM 2,4-pyridine dicarboxylic acid (2,4-PDCA)...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309001833
更新日期:1993-06-01 00:00:00
abstract:PURPOSE:To determine the roles of the murine interleukin-8 receptor homolog (mIL-8Rh, neutrophil chemokine CXC receptor 2) and macrophage inflammatory protein-1alpha (MIP-1alpha, a CC chemokine) in two eye inflammation models: endotoxin-induced uveitis (EIU) and immune complex-induced uveitis (reverse passive Arthus re...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.1.76.5339
更新日期:1999-07-01 00:00:00
abstract::To better define the growth requirements for corneal epithelial cells, methods for serum-free culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999564
更新日期:1990-09-01 00:00:00
abstract::We adapted a previously described procedure to quantitate collagen in corneal tissue sections prepared from paraffin-embedded samples. The method entailed staining the deparaffinized tissue sections with Sirius red, eluting the bound dye with NaOH-methanol, and estimating the color in a spectrophotometer as an indicat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609015119
更新日期:1986-08-01 00:00:00
abstract::A model for endothelial transport is updated to include recent evidence. We discuss electrolyte movements based on a Na+-K+ ATPase, a Na+-H+ exchanger, a Na+-HCO3 coupler, a Cl- -HCO-3 exchanger, a K+-Cl-coupler, and K+ and anion channels. We discuss near-isotonic transport of fluid on the basis of recent findings of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509025148
更新日期:1985-04-01 00:00:00
abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.669005
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.1.26.5116
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:To determine the plasma levels of the sulfur-containing amino-acids homocysteine, cysteine, cysteinylglycine, glutamylcysteine, glutathione, and taurine in patients with central retinal vein occlusion (CRVO) and in healthy subjects and to ascertain whether there are statistically significant differences between...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003698863
更新日期:2010-07-01 00:00:00
abstract:PURPOSE:This study was undertaken in order to investigate whether the production of vasodilatory prostaglandins is increased in the retinal vasculature of the diabetic rat. METHODS:Diabetes was induced in male Sprague-Dawley rats using streptozotocin. Diabetic rats were left uncontrolled for 3-4 weeks or were treated ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.2.79.5386
更新日期:1999-02-01 00:00:00
abstract:PURPOSE/AIM:We aimed to characterize the connective tissue microanatomy, elastin abundance, and fiber orientation in the human optic nerve sheath, also known as the optic nerve dura mater, for correlation with its biomechanical properties. MATERIALS AND METHODS:Seven whole human orbits aged 4-93 years, and five isolat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1739314
更新日期:2020-07-01 00:00:00
abstract:PURPOSE/AIMS:To evaluate the prognostic factors for the best corrected visual acuity (BCVA) and central subfield macular thickness (CSMT) after vitrectomy with internal limiting membrane (ILM) peeling for diabetic macular edema. MATERIALS AND METHODS:A total of 44 eyes of 35 patients who had undergone vitrectomy with ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.820327
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:To evaluate the effect of gatifloxacin ophthalmic solution 0.3% (Zymar, Allergan, Inc., Irvine, CA, USA) on corneal endothelial cell density and morphology and to measure gatifloxacin penetration into aqueous humor. METHODS:This was a single-center, open-label clinical study. Ten patients undergoing standard c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590968556
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:We identified a novel mutation in Paired Box gene 6 (PAX6) and characterized its associated clinical features of severe ocular malformation in a Chinese family with congenital aniridia. METHODS:We studied two patients with aniridia from a Chinese family. All patients and noncarriers in this family underwent fu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.688165
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:The aim of this study is to determine age-related morphological changes in the corneal subbasal nerve plexus (SNP) in two inbred mouse strains. MATERIALS AND METHODS:The corneal SNP was investigated by in vivo confocal laser scanning microscopy (CLSM) in 0.5-, 1-, 1.5-, and 2-year-old C57BL/6J mice and in 0.5-...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1088952
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:To evaluate the immediate effect of 3% diquafosol ophthalmic solution on tear MUC5AC concentration, periodic acid-Schiff (PAS)-positive goblet cells, and tear film stability in normal and keratoconjunctivitis sicca (KCS) rat models. METHODS:Rats were divided into normal and KCS groups. 3% of diquafosol solutio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1233986
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Choroidal neovascularization (CNV) is a major cause of vision loss in patients with age-related macular degeneration (AMD). Stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) plays a critical role in homing of bone marrow-derived cells (BMCs) to choroidal neovascularization (CNV). In this st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.593107
更新日期:2011-09-01 00:00:00
abstract::Retinal degeneration is a leading cause of untreatable blindness in the industrialised world. It is typically irreversible and there are few curative treatments available. The use of stem cells to generate new retinal neurons for transplantation purposes has received significant interest in recent years and is beginni...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1669665
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:Predicting the toxic potential of compounds to the ocular surface has depended on the Draize test for the past half century. Alternatives to Draize testing have recently been sought for a number of reasons. Stress gene expression has emerged as a means of quantifying cellular reaction and, thus, the toxic poten...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.115.5325
更新日期:1999-08-01 00:00:00
abstract::Achieving the long-term goal of successful engraftment of retinal tissues into eyes blinded by endogenous retinal failure will depend, at least in part, on controlling the immune response to antigens expressed on retinal grafts. Since histoincompatible tissues will have to be used for such transplants, methods to cont...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209000743
更新日期:1992-07-01 00:00:00
abstract:PURPOSE:To examine the effect of elevated extracellular glucose, thus simulating diabetes, on migration, adhesion and proliferation of SV40 transformed human corneal epithelial (HCE) cells. METHODS:HCE cells were maintained in serum supplemented media containing 5 mM, 17.5 mM or 38 mM D-glucose. Cell migration was det...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.9.924.5133
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:We report on the use of subconjunctival bevacizumab on corneal vessel density in recurrent pterygia. METHODS:The charts of 5 patients with recurrent pterygium, who received subconjunctival injections of bevacizumab (2.5 mg/0.1 ml) were retrospectively reviewed. Ophthalmic evaluation included Snellen visual acu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701799101
更新日期:2008-01-01 00:00:00
abstract::We previously reported that Ia+ monolayers of LEW rat corneal endothelial (CE) cells were unable to stimulate proliferation of MHC compatible T cell lines or IL-2 release from hybridomas, and inhibited [3H]-thymidine incorporation when added to conventional lymphocyte proliferation assays. Our purpose was to further a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999891
更新日期:1994-08-01 00:00:00
abstract::With Golgi impregnation, a simple amacrine cell type is described in the chick retina. The most relevant morphological feature of these retinal neurons is a single straight, radial trunk which dichotomizes tangentially in a rectilinear expansion in the 5th stratum of the inner plexiform layer (IPL). ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908995187
更新日期:1989-02-01 00:00:00
abstract::Intraocular synthesis of IgG antibodies against HSV (herpes simplex virus), CMV (cytomegalovirus) and VZV (varicella zoster virus) is considered as an indirect proof of uveoretinal infection. Paired serum and aqueous samples obtained from 16 patients with retinitis associated with AIDS, 3 patients with ARN, 8 patients...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999412
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:To evaluate transcorneal penetration of topically applied 0.5% levofloxacin into the aqueous humor in human eyes. METHODS:Twenty cataract patients (14 females, 6 males) received 3 drops of 0.5% levofloxacin at 15 min intervals from 90 minutes before the surgery. At the beginning of the cataract surgery, 50 mic...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.5.403.8517
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:Ethacrynic acid (ECA) has been shown to increase facility of aqueous outflow in whole eyes and perfused anterior segments, to open up spaces between cells in the trabecular meshwork and inner wall of Schlemm's canal, and to cause separation and retraction of trabecular meshwork and endothelial cells in culture....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017644
更新日期:1996-09-01 00:00:00
abstract:PURPOSE:Extracellular matrix (ECM) plays an important role in the regulation of cell function. The aging process may involve chemical modifications to ECM proteins, which may contribute to the aging of the Bruch membrane and pathogenesis of age-related macular degeneration (AMD). The purpose of this study is to investi...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590968259
更新日期:2005-08-01 00:00:00