Abstract:
PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After anesthesia, 27 eyes of pigmented rats received an intraperitoneal injection of 0.2 ml of, 10% sodium fluorescein 15 minutes prior to laser treatment. With a blue-green argon laser, selected venous sites next to the optic nerve head were photocoagulated indirectly with a 78 diopter lens. Venous occlusion was accomplished using laser parameters of 1.0 second, 50 microns, and 50-100 mW. For a control group, 10 eyes were coagulated on the retina between major vessels using the same parameters after fluorescein injection. For a second control group, 1% sodium hyaluronate was injected into the subretinal space to make a long-standing retinal detachment in 5 eyes. RESULTS:With 1-8 laser impulses, each venous occlusion was obtained and was associated with extreme venous constriction and tortuousity. Retinal edema became evident 10-30 minutes after treatment in the sectors associated with the occluded veins. This edema became a bullous retinal detachment (RD) within 12 hours and intra-retinal hemorrhage was observed. The retinal edema continued for 3-10 days and the retinas reattached spontaneously. Prior to or after retinal reattachment 70% (19/27) of eyes developed retinal NV and tractional RD. Of these, 11 developed NV of the optic disc (NVD), 6 developed NV elsewhere (NVE), and 2 developed NVD and NVE. In 30% (8/27) of the eyes, retinal edema resolved without evidence of NV. In control groups no eyes showed either circulatory disorders or evidence of NV. CONCLUSIONS:This is a new model of retinal ischemia and associated neovascularization established by venous thrombosis that is easily reproducible. Many aspects of rat retinal physiology are known and this model has promise as an avenue for further investigation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Saito Y,Park L,Skolik SA,Alfaro DV,Chaudhry NA,Barnstable CJ,Liggett PEdoi
10.1076/ceyr.16.1.26.5116subject
Has Abstractpub_date
1997-01-01 00:00:00pages
26-33issue
1eissn
0271-3683issn
1460-2202journal_volume
16pub_type
杂志文章abstract:PURPOSE:To investigate the morphological or functional differences of retina in amblyopia. MATERIALS AND METHODS:Forty-one patients with unilateral strabismic, anisometropic or combined amblyopia were included in the study. A control group was composed of 16 normal children. All participants were tested with optical c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.779721
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To compare the abilities of balanced salt solution, BSS Plus and Hartmann's lactated Ringer's (HLR) solution to maintain the viability of retinal vascular endothelial cells (RVEC) in vitro. METHODS:Cultured retinal vascular endothelial cells were suspended in each irrigating solution for four hours. Viability ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.3.239.15414
更新日期:1997-03-01 00:00:00
abstract::The cell content of the precorneal tears of eight subjects was sampled following overnight wear of a contact lens in one eye. This was compared with morning samples taken from the other eye, and afternoon samples taken several days later from both eyes. In the morning the median number of desquamated epithelial cells ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908996380
更新日期:1989-04-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) were used to model the solar radiation-induced cataract that occurs in hatchery-reared lake trout (Salvelinus namaycush). Rainbow trout eyes were exposed to wideband predominantly UVB radiant energy from a filtered 1000W xenon source. Lenticular damage was assessed and classified an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999477
更新日期:1993-02-01 00:00:00
abstract::We established growth curves for Propionibacterium acnes isolates recovered from eyes with chronic postoperative endophthalmitis. The growth curve plotted the average of the duplicate bacterial concentration against time. The generation time for P. acnes calculated from the growth curves was approximately 5.1 hours. T...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999875
更新日期:1994-06-01 00:00:00
abstract::Phosphorylated proteins of the chick lens were identified following incubation of lenses in a medium containing 32P and subsequent analysis by gel electrophoresis. The acidic variant of the vimentin and both subunits of fodrin were phosphorylated, as were the 95 Kd and 49 Kd proteins associated with the beaded-chain f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409167214
更新日期:1984-07-01 00:00:00
abstract:PURPOSE:To determine, by a plaque reduction assay, the in vitro efficacy of novel antiviral agents in the treatment of feline herpes virus 1 (FHV-1) keratitis in the domestic cat (Felis felis). MATERIALS AND METHODS:A standard plaque reduction assay was performed using a laboratory strain of FHV-1 and embryo-derived f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490504849
更新日期:2004-08-01 00:00:00
abstract::Purpose: To investigate to what extent the OSDI can be utilized as a discriminative test for clinical findings. Methods: One thousand and ninety patients with dry eye disease (DED) were consecutively included and examined for osmolarity, tear film break-up time (TFBUT), ocular protection index (OPI), ocular surface st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1604972
更新日期:2019-09-01 00:00:00
abstract::It has been suggested that the provision of HLA matched tissue for corneal transplantation would be beneficial for graft survival especially in high risk patients. In this study we report the application of a polymerase chain reaction (PCR) based tissue typing procedure to type cadaveric donor material. Using beta-glo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209001798
更新日期:1992-05-01 00:00:00
abstract:PURPOSE:Recent studies have shown that the levels of interleukin (IL)-1beta, IL-6 and IL-8 are associated with the severity of infectious diseases. The purpose of this study was to investigate whether IL-1beta regulates the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.6.406.6969
更新日期:2001-12-01 00:00:00
abstract::Corneas and tendons dissected from 17-day-old chick embryos were labeled with [35S]methionine in the presence of 0.3 mM alpha,alpha'-dipyridyl. The unhydroxylated, 35S-labeled pro alpha chains and alpha chains were isolated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The pro alpha and alpha chains...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688508999972
更新日期:1985-02-01 00:00:00
abstract::The variation of central corneal thickness with age was measured weekly at 10am by ultrasound pachometry in 10 normal (5 to 19 weeks of age) and 10 alloxan-induced diabetic (9 to 19 weeks of age) rabbits. The diurnal variation of corneal thickness was also measured at 2 hour intervals for a period of 24 hours on both ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999611
更新日期:1990-05-01 00:00:00
abstract:PURPOSE:Transforming growth factor -beta2 (TGF-beta2) is a predominant isoform of TGF-betas in the eye and plasmin is a peptidase with many functions. To better understand the pathogenesis of retinal microcirculation disorders, the effects of TGF-beta2 and plasmin on cultured bovine retinal pericytes were investigated....
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Samples of aqueous humor from rabbits and humans were examined for growth effects on subconfluent monolayer fibroblast cultures from ocular and extraocular tissues. At a concentration of 20% aqueous by medium volume, both homologous and heterologous aqueous stimulated DNA synthesis in fibroblasts from both species. Th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209020019
更新日期:1982-01-01 00:00:00
abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001869
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To compare the efficacy and tolerability of diclofenac and bromfenac ophthalmic solutions as adjunctive therapy after cataract surgery. MATERIALS AND METHODS:This prospective randomized controlled study included 130 patients who underwent cataract surgery. One hundred patients were randomized to receive postop...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2018.1501489
更新日期:2018-12-01 00:00:00
abstract:UNLABELLED:Abstract Purpose of the Study: Protein tyrosine phosphatase σ (PTPσ) acts as a neuronal receptor for chondroitin sulfate proteoglycans (CSPGs). CSPGs have inhibitory effects on experience-dependent plasticity and usually form lattice-like cell coatings that surround the parvalbumin (PV) interneurons in the v...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.803287
更新日期:2013-10-01 00:00:00
abstract:PURPOSE/AIM:Cultured autologous oral mucosal epithelial cells (OMECs) have proven useful in the treatment of ocular surface disorders. This study is the first to investigate the potential of expanding OMEC in a xenobiotic- and serum-free medium using therapeutic contact lenses (CLs) as a substrate and carrier. MATERIA...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1004720
更新日期:2016-01-01 00:00:00
abstract::Approximately 90% of all ophthalmic drug formulations are now applied as eyedrops. Although eyedrops are convenient and well accepted by patients, about 95% of the drug contained in the drops is lost due to absorption through the conjunctiva or through the tear drainage. A major fraction of the drug eventually enters ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500346633
更新日期:2005-12-01 00:00:00
abstract:PURPOSE:To investigate the therapeutic role of topical and oral fluconazole treatment using a rabbit model of Aspergillus fumigatus keratitis. METHODS:Aspergillus fumigatus spores were injected into the corneal stroma of the right eye of 20 rabbits. Forty-eight hours later the rabbits were randomly divided into three ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.2.113.14514
更新日期:2003-02-01 00:00:00
abstract:PURPOSE:Methotrexate (MTX) is the most commonly used chemotherapeutic agent to treat primary central nervous system lymphoma (PCNSL) and intraocular lymphoma (IOL). 2-methoxyestradiol (2ME2) is a potent antitumor and anti-angiogenesis agent which, unlike other cytotoxic drugs, has minimal toxicity. In this study, anti-...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701473244
更新日期:2007-07-01 00:00:00
abstract:PURPOSE:To investigate the agreement of angular locations of retinal nerve fiber layer (RNFL) defect margins in glaucomatous eyes by using red-free fundus photographs and Cirrus high-definition optical coherence tomography (OCT) RNFL deviation and thickness maps. METHODS:We examined 380 RNFL defects that showed clear ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.900805
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:To determine whether substance P is present in human tears. METHODS:Tear samples (1-2 microliters) were collected from one eye of each of 12 subjects. Two of the eyes had dry eye syndrome, two wore contact lenses and had dry eye syndrome, and eight were normal. Five of the eight normal eyes were scheduled to u...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.9.960.5040
更新日期:1997-09-01 00:00:00
abstract::This study reports on serum interleukin 2-receptor (IL-2R) levels in uveitis patients. IL-2R is the mediator protein of interleukin 2 (IL-2), a mitogenetic cytokine of primarily immune cell interaction. Stimulated T-cells readily express IL-2R on their cell surface and release a soluble form into serum. The measuremen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999415
更新日期:1990-01-01 00:00:00
abstract::Penetrating keratoplasty was the only therapeutic choice for the treatment of corneal endothelial decompensation until the introduction of evolutional endothelial keratoplasties, namely Descemet's stripping automated endothelial keratoplasty (DSAEK) and Descemet's membrane endothelial keratoplasty (DMEK). Although now...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1700529
更新日期:2020-03-01 00:00:00
abstract::The effect of monensin on the cytotoxic effect of antitransferrin receptor immunotoxin (IT) was determined on cultured, human retinal pigment epithelial (hRPE) cells. Human RPE cells were treated with 0.1-10,000 ng/ml IT with and without 0.01-0.1 microM monensin, a lysosomotropic reagent that can influence IT activity...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999495
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:Corneal scarring is a common poor outcome of corneal trauma. Transforming growth factor β-1 plays a vital role in corneal fibrosis, inducing keratocyte transformation to myofibroblasts. Other than corneal transplantation, no other curative treatment methods for corneal scarring are currently available. Hypercap...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1820526
更新日期:2020-09-11 00:00:00
abstract::The ocular penetration pathways of three alpha 2-adrenergic agents (p-aminoclonidine, AGN 190342, and clonidine) were investigated in rabbits both in vitro and in vivo. The corneal permeabilities of the compounds correlated positively with their octanol/water distribution coefficients. The ocular drug absorption via c...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997579
更新日期:1990-11-01 00:00:00
abstract:PURPOSE:Extracellular matrix homeostasis is dependent in part upon a family of matrix metalloproteinases and their specific inhibitors, the tissue inhibitors of metalloproteinases (TIMPs). Recently, gene defects in TIMP-3 have been identified in the affected individuals of several families with Sorsby's fundus dystroph...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.2.102.5086
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:To determine the effects of a subconjunctival injection of mitomycin-C (MMC) on iridial circulation in rabbits. METHODS:Dutch rabbits anesthetized with pentobarbital received a 0.2-ml subconjunctival injection of 0.4 mg/ml or 0.1 mg/ml MMC; the contralateral eye received 0.2 ml physiological saline. Intraocula...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.197.5327
更新日期:1999-08-01 00:00:00