Abstract:
PURPOSE:Methotrexate (MTX) is the most commonly used chemotherapeutic agent to treat primary central nervous system lymphoma (PCNSL) and intraocular lymphoma (IOL). 2-methoxyestradiol (2ME2) is a potent antitumor and anti-angiogenesis agent which, unlike other cytotoxic drugs, has minimal toxicity. In this study, anti-proliferative, apoptotic, and cell-cycle effects of 2ME2 and MTX were compared to evaluate 2ME2 efficacy in human lymphoma cells, models for non-Hodgkin B cell lymphomas. METHODS:The cells were cultured and incubated with varying concentrations of 2ME2 or MTX. A tetrazolium-based colorimetric assay was used to quantify the anti-proliferative effects of 2ME2 and MTX using a microplate reader. To detect apoptotic and cell cycle distribution changes induced by 2ME2 and MTX, the cells were stained with Annexin V-FITC and/or propidium iodide (PI) and analyzed by flow cytometry. RESULTS:Lymphoma cell proliferation was inhibited by 50% at concentrations ranging from 0.4 to 1 microM for 2ME2 and 0.06 to 0.2 microM for MTX. Induction of apoptosis by 2ME2 and MTX was observed in the tested cells. 2ME2 was a G2/M-phase specific blocker whereas MTX was an S-phase specific blocker in cell cycle analyses. At 1 microM concentration, 2ME2 and MTX showed similar anti-proliferative effect on the lymphoma cell lines. In previously reported studies, for normal endothelial cells, 1 microM 2ME2 showed no appreciable toxicity, while MTX at this same concentration exhibited significant cytotoxicity. 2ME2 at a therapeutic target concentration of 1 mu M may be an effective and relatively non-toxic drug for the treatment of PCNSL with IOL. CONCLUSIONS:Our study of the effect of 2ME2 and MTX on anti-proliferation, apoptosis, and cell cycling suggests that 2ME2 is a potential agent for treating PCNSL and IOL.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Park J,Franco RS,Augsburger JJ,Banerjee RKdoi
10.1080/02713680701473244subject
Has Abstractpub_date
2007-07-01 00:00:00pages
659-67issue
7-8eissn
0271-3683issn
1460-2202pii
781950572journal_volume
32pub_type
杂志文章abstract:PURPOSE:Whether there is an interaction between eyes of individual subjects in refractive development is an important issue to guide experimental designs and help understand mechanisms involved in development of refractive errors. This study investigated whether spontaneous high myopia in one eye will affect refractive...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.568660
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells ar...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:Mechanical stimulation induces intercellular Ca(2 +) waves in the corneal endothelium. The extent of the wave propagation is dependent on the activity of gap junctions, hemichannels, and ectonucleotidases. To further establish the use of a cell culture model to investigate intercellular communication, in this s...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680902913022
更新日期:2009-06-01 00:00:00
abstract::Antifibrosis agents have improved the success of glaucoma filtration surgery, although undesired side effects are not readily reversible and may present a major limitation in the use of these agents. Our purpose was to study the efficacy of cytosine arabinoside (Ara-C) as an adjunctive antimetabolite in glaucoma surge...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409025140
更新日期:1994-11-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) were used to model the solar radiation-induced cataract that occurs in hatchery-reared lake trout (Salvelinus namaycush). Rainbow trout eyes were exposed to wideband predominantly UVB radiant energy from a filtered 1000W xenon source. Lenticular damage was assessed and classified an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999477
更新日期:1993-02-01 00:00:00
abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514055
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:To evaluate differences of phagocytic capacities in rat retinal pigment epithelial (RPE) cells, iris pigment epithelial (IPE) cells, and basic fibroblast growth factor (bFGF) cDNA-transfected IPE (bFGF-IPE) cells in vitro. METHODS:The RPE cells and IPE cells were isolated from adult Long Evans rats' eyes. The ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.3.185.5465
更新日期:2001-09-01 00:00:00
abstract::Specimens of a cornea, conjunctiva and iris were removed at the time of penetrating keratoplasty in a case of nephropathic cystinosis. Immunohistochemical studies revealed the presence of class II antigens on the stromal keratocytes of the cornea and the vascular endothelial cells, pericytes and fibroblasts of the con...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709025222
更新日期:1987-04-01 00:00:00
abstract:PURPOSE:Pseudoexfoliation (PXF) is a microfibrillopathy involving disordered elastogenesis. Abnormal extracellular matrix (ECM) production underlies the pathophysiology of PXF. The enzyme Lysyl oxidase (LOX) and its isoforms are known to cross-link the elastin and collagen. Though the etiopathogensis of PXF is not well...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1125505
更新日期:2016-10-01 00:00:00
abstract:PURPOSE:RPE65, a retinal pigment epithelium-specific 65-kDa protein, plays a critical role in the visual cycle of the eye. Rpe65(-/-) mice develop vision loss due to a lack of 11-cis-retinal, degradation of M-opsin and mislocalization of S-opsin. Several studies have suggested that 9-cis-β-carotene, a precursor of 9-ci...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.907430
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:To demonstrate spectroscopic retinal oximetry measurements on arteries and veins in swine using intravitreal illumination. Retinal arterial and venous saturations are measured for a range of inspired O2 levels after pars plana vitrectomy. METHODS:Pars plana vitrectomy and intravitreal manipulations were perfor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600760493
更新日期:2006-07-01 00:00:00
abstract::The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003066
更新日期:1984-04-01 00:00:00
abstract::We examined jun-B gene expression in the rat retina after a focal retinal injury by in situ hybridization. 30 min after producing a penetrating retinal wound, the retina showed the expression of jun-B mRNA intensively in the inner nuclear layer (INL) and relatively sparsely in the ganglion cell layer. The double-label...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508998524
更新日期:1995-11-01 00:00:00
abstract::Blood and lymphatic vessel proliferation into the cornea occurs after severe trauma. Whilst the events early in blood vessel growth are reasonably understood, little is known of the early events in lymphangiogenesis. In this study, a model for proliferating lymphatic vessels using tritiated-thymidine autoradiography a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013898
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:Previous studies have suggested that corneal fibrosis controlled by a TGFbeta-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFbeta in post-PR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::The water soluble (WS), urea soluble (US) and urea insoluble (UI) fractions from individual human lenses 1.8 to 65 years of age were isolated from concentric fiber layers. In lenses younger than 19 years, a uniform distribution in the amount of WS, US and UI fractions was found throughout the entire lens. These fracti...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609015101
更新日期:1986-02-01 00:00:00
abstract::Purpose: To investigate the cytotoxic effects of fresh black carbon (FBC) particles and ozone-oxidized BC (OBC) particles on human corneal epithelial cells (HCECs), and the role of the NLRP3 inflammasome signal pathway in the process.Methods: HCECs were treated with different doses of FBC and OBC particles, the cell i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1698051
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:To compare the differences in higher-order aberrations (HOAs) after laser subepithelial keratomileusis (LASEK) between two different laser platforms. METHODS:One hundred and seven eyes of 107 patients were included in this study. Fifty-six eyes underwent LASEK with the Triple-A profile (an ablation profile of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1726404
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To evaluate the effect of short-term hypobaric hypoxic conditions (STHC) on intraocular pressure (IOP) in healthy subjects. METHODS:The study group was comprised of 30 healthy participants (60 eyes) with a mean age of 24.8 years. IOP was measured with a Tono-Pen XL tonometer at ground level (792 m above sea le...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802416696
更新日期:2008-10-01 00:00:00
abstract::Lens epithelial cells were isolated from samples of C57BL/6 and CD-1 mice at different ages between 1 and 5 months, and assayed for the level of N-acetyl-beta-glucosaminidase and their ability to replicate in-vitro. The lens epithelial cells from C57BL/6 mice had a reduced replicative potential compared to other norma...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688208996354
更新日期:1982-01-01 00:00:00
abstract::Inoculation of herpes simplex virus Type 1 into the anterior chamber of BALB/c mice induces a characteristic ocular infection involving primarily the anterior segment of virus-injected eyes and the retina of contralateral uninjected eyes. In the BALB/c strain, which is susceptible to herpetic retinitis, anti-HSV delay...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688808995741
更新日期:1988-02-01 00:00:00
abstract:PURPOSE/AIM:To quantitatively analyse the evolution of TUNEL-labeling, after in vivo exposure to UVB. METHODS:Altogether, 16 Sprague Dawley rats were unilaterally exposed in vivo for 15 min to close to threshold dose, 5 kJ/m(2), of ultraviolet radiation in the 300 nm wavelength region. Animals were sacrificed in group...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.783079
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.1.26.5116
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:To learn if peripheral nerve pathways are necessary for corneal expansion and anterior segment growth under a 12-hr light:dark cycle or for the inhibition of corneal expansion under constant light rearing. METHODS:Recently hatched White Leghorn chicks under anesthesia received unilateral ciliary ganglionectomy...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600738127
更新日期:2006-06-01 00:00:00
abstract:PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1250278
更新日期:2017-05-01 00:00:00
abstract::In primates, ciliary muscle contraction causes accommodation and facilitates aqueous outflow. In living rhesus monkeys, accommodative, outflow facility, and ciliary muscle movement responses to cholinergic agonists all decline with age. We developed an apparatus to determine in vitro whether the latter is related to i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309024623
更新日期:1993-05-01 00:00:00
abstract::Changes in the content of calcitonin gene-related polypeptide (CGRP) and tyrosine hydroxylase (TH) immunoreactivities in the anterior segment of the rat eye were assessed histochemically in animals subjected to sympathetic ganglionectomy or to chronic sensory denervation induced by neonatal capsaicin treatment. In the...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033207
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:To determine whether environmental factors or bacterial viability affect the binding of two strains of P. aeruginosa to mouse cornea. METHODS:Scarified corneas were placed in organ culture and inoculated with P. aeruginosa cell suspensions containing either ATCC 19660 or PAO1 bacterial strains classed as cytot...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.3.231.5226
更新日期:1998-03-01 00:00:00
abstract:PURPOSE:To evaluate choroidal thickness (CT) and retinal morphological changes in eyes with neovascular age-related macular degeneration (nAMD) following ranibizumab or aflibercept intravitreal treatment. MATERIALS AND METHODS:This was a prospective, observational, comparative study where 76 eyes of 76 consecutive pat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405045
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Endothelial cells of different vascular systems may express site-specific adhesion molecules to attract leukocyte subsets. This study describes a method to visualize and compare leukocyte-endothelial interactions in three vascular beds within the same eye in mice. METHODS:Digital in vivo fluorescence microscop...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.3.214.8303
更新日期:2002-03-01 00:00:00