Reduced intercellular communication and altered morphology of bovine corneal endothelial cells with prolonged time in cell culture.


PURPOSE:Mechanical stimulation induces intercellular Ca(2 +) waves in the corneal endothelium. The extent of the wave propagation is dependent on the activity of gap junctions, hemichannels, and ectonucleotidases. To further establish the use of a cell culture model to investigate intercellular communication, in this study, we have characterized the changes in the Ca(2 +) wave propagation in bovine corneal endothelial cells with prolonged time in culture. MATERIALS AND METHODS:Freshly isolated BCEC were cultured for a short term (8 to 14 days; referred to as "short term") and a long term (21 to 30 days; referred to as "long term"). Cell surface area and size were measured by confocal microscopy and flow cytometry, respectively. Calcium wave propagation was assayed by imaging spread of the Ca(2 +) waves elicited by mechanical stimulation. ATP release was assayed using Luciferin-Luciferase bioluminescence technique. RESULTS:Cells cultured for a long term showed larger surface area and size compared to those cultured for a short term, but a reduced spread of the Ca(2 +) wave. Exposure to exogenous apyrases, which can rapidly hydrolyze extracellular ATP, reduced the spread of the Ca(2 +) wave in both groups. The fractional decrease, however, was smaller in cells cultured for a long term. Exposure to ARL-67156 to inhibit the ectonucleotidases led to a larger enhancement of the active area in cells cultured for a long term. However, the active areas of the two groups were not significantly different in the presence of the drug. Furthermore, ATP release in response to mechanical stimulation was lower in cells cultured for a long term in the absence of ARL-67156 but not in its presence. CONCLUSIONS:BCEC cultured for a long term show an increase in cell surface area and cell size similar to the effect of aging in human corneas. Moreover, the cells cultured for a long term showed a reduced ATP-dependent paracrine intercellular communication, largely due to an increase in the activity of the ectonucleotidases.


Curr Eye Res


Current eye research


D'hondt C,Ponsaerts R,Srinivas SP,Vereecke J,Himpens B




Has Abstract


2009-06-01 00:00:00














  • Target Properties Effects on Central versus Peripheral Vertical Fusion Interaction Tested on a 3D Platform.

    abstract:PURPOSE:We investigated the impact of target properties on vertical fusion amplitude (VFA) using a 3D display platform; the performance of the subjects allowed us to assess how central and peripheral retina regions interact during the fusion process. MATERIAL AND METHODS:Fourteen subjects were involved in the test. VF...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Zhang D,Neveu P,Fattakhova Y,Ferragut S,Lamard M,Cochener B,de Bougrenet de la Tocnaye JL

    更新日期:2017-03-01 00:00:00

  • Effect of a single-piece and a three-piece acrylic sharp-edged IOL on posterior capsule opacification.

    abstract:BACKGROUND:To compare the intensity of posterior capsule opacification (PCO) between two intraocular lenses (IOLs) of the same optic material, but with different haptic design (1-piece vs. 3-piece). METHODS:This prospective intraindividual controlled cohort study comprised eighty eyes of 40 patients with bilateral age...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验


    authors: Mylonas G,Prskavec M,Baradaran-Dilmaghani R,Karnik N,Buehl W,Wirtitsch M

    更新日期:2013-01-01 00:00:00

  • Slitlamp assessment of age of onset and incidence of cataracts in pink-eyed, tan-hooded retinal dystrophic rats.

    abstract::Posterior subcapsular cataracts (PSC) are associated with hereditary retinal dystrophy in the Royal College of Surgeons (RCS) rat model and with human retinitis pigmentosa. The relationship of lens and retinal pathology has never been explained. Previous studies of pink-eyed RCS rats aged 2.5 to 11 months had shown an...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Hess HH,Newsome DA,Knapka JJ,Westney GE

    更新日期:1982-01-01 00:00:00

  • Neuroprotective Effects of Psalmotoxin-1, an Acid-Sensing Ion Channel (ASIC) Inhibitor, in Ischemia Reperfusion in Mouse Eyes.

    abstract:PURPOSE:The purpose of the current study is to assess changes in the expression of Acid-Sensing Ion Channel (ASIC)1a and ASIC2 in retinal ganglion cells (RGCs) after retinal ischemia and reperfusion (I/R) injury and to test if inhibition of ASIC1a provides RGC neuroprotection. METHODS:Transient ischemia was induced in...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Dibas A,Millar C,Al-Farra A,Yorio T

    更新日期:2018-07-01 00:00:00

  • Apoptosis-related fas antigen on memory T cells in aqueous humor of uveitis patients.

    abstract::To investigate the role of lymphocytes in the pathogenesis of uveitis, we analyzed the expression of memory markers, CD29 and CD45RO antigens, and apoptosis-related Fas antigen on T lymphocytes in the aqueous humor (AH) and peripheral blood (PB) from patients with uveitis. Using three-color flow cytometry, we assessed...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Ohta K,Norose K,Wang XC,Ito S,Yano A,Segawa K

    更新日期:1996-03-01 00:00:00

  • Anterior segment growth and peripheral neural pathways in chick.

    abstract:PURPOSE:To learn if peripheral nerve pathways are necessary for corneal expansion and anterior segment growth under a 12-hr light:dark cycle or for the inhibition of corneal expansion under constant light rearing. METHODS:Recently hatched White Leghorn chicks under anesthesia received unilateral ciliary ganglionectomy...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Stone RA,Sugimoto R,Capehart C,Pendrak K,Lin T

    更新日期:2006-06-01 00:00:00

  • A human apolipoprotein E mimetic peptide effectively inhibits HSV-1 TK-positive and TK-negative acute epithelial keratitis in rabbits.

    abstract:PURPOSE:To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis. MATER...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Bhattacharjee PS,Neumann DM,Hill JM

    更新日期:2009-02-01 00:00:00

  • Intentional reactivation of latent ocular herpes infection during BVDU therapy.

    abstract::Sixteen adult New Zealand white rabbits with previously confirmed herpes simplex virus type 1 (HSV-1) infections were stimulated by iontophoresis of 6-hydroxydopamine into the cornea and were followed-up by topical epinephrine treatment to confirm latency. A total of 224 ocular cultures were obtained, of which 73 were...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Trousdale MD,Robin JB,Willey DE,De Clercq E

    更新日期:1987-12-01 00:00:00

  • Effect of Congenital Lower Eyelid Epiblepharon Surgery on Asymmetric Margin Reflex Distance 1.

    abstract::Objectives: To investigate the characteristics of margin reflex distance 1 (MRD1) asymmetry in congenital lower eyelid epiblepharon and its resolution after surgical correction of epiblepharon. Methods: Among patients who underwent lower eyelid epiblepharon surgery from November 2015 to September 2017, patients with a...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Yang MK,Kim N,Choung HK,Khwarg SI

    更新日期:2020-12-15 00:00:00

  • Expression of membrane-associated mucins in limbal stem cell deficiency and after transplantation of cultivated limbal epithelium.

    abstract:PURPOSE:Membrane-associated mucins are important components of the ocular tear film. Our objective was to characterize the expression of membrane-associated mucins MUC1, MUC4, and MUC16 in healthy cornea, healthy conjunctiva, fibrovascular tissue (pannus) covering the corneal surface in limbal stem cell deficiency (LSC...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Pauklin M,Kakkassery V,Steuhl KP,Meller D

    更新日期:2009-03-01 00:00:00

  • Oxidative Stress Induces Biphasic ERK1/2 Activation in the RPE with Distinct Effects on Cell Survival at Early and Late Activation.

    abstract:PURPOSE:Oxidative stress is considered a major factor in the deterioration of retinal pigment epithelium (RPE) cells in dry age-related macular degeneration (AMD). The MAPK ERK1/2 can be activated by oxidative stress, may exert both pro- and anti-apoptotic functions, and has recently been proposed as a major factor in ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Koinzer S,Reinecke K,Herdegen T,Roider J,Klettner A

    更新日期:2015-01-01 00:00:00

  • Is another mannose receptor-like lectin present in retinal pigment epithelium?

    abstract:PURPOSE:This study was designed to investigate the presence of the secretory phospholipase A2 receptor in RPE, a protein closely related to the phagocytic mannose receptor. METHODS:Proteins from cultured rat, pig, and human RPE were separated by SDS-PAGE and immunoblotted with a polyclonal guinea pig sPLA2 receptor an...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Wilt SD,McLaughlin BJ

    更新日期:1999-07-01 00:00:00

  • Characterization of human meibum lipid using raman spectroscopy.

    abstract:PURPOSE:Raman spectroscopy could potentially be used as a diagnostic tool for the detection of meibomian gland dysfunction by measuring the composition, conformation, and amount of meibum lipid on the lid margin. Toward this goal and to test our studies of meibum lipid using infrared spectroscopy, we measured the Raman...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Oshima Y,Sato H,Zaghloul A,Foulks GN,Yappert MC,Borchman D

    更新日期:2009-10-01 00:00:00

  • Calpain activity in the retinas of normal and RCS rats.

    abstract::Calpains are calcium-activated proteinases which have been implicated in tissue differentiation and degeneration. The aims of the present study were: (1) to determine the relationship between postnatal age and calpain activity in the rat retina; (2) to test if calpain activity was aberrant in the RCS retina at differe...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Azarian SM,Williams DS

    更新日期:1995-08-01 00:00:00

  • C1r-C1s-C1inhibitor (C1rs-C1inh) complex measurements in tears of patients before and after penetrating keratoplasty.

    abstract:PURPOSE:The aim of this pilot study was to determine the presence of complement activation products in tears from pre- and postkeratoplasty eyes and the fellow eyes in order to investigate the activation of the classical and alternative pathways of the complement system in the early postkeratoplasty period. METHODS:Te...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kerényi A,Nagy G,Veres A,Varga L,Füst A,Nagymihány A,Czumbel N,Süveges I,Füst G

    更新日期:2002-02-01 00:00:00

  • Peptide-maps of procollagen (I) from corneas and tendons of 17-day-old chick embryos.

    abstract::Corneas and tendons dissected from 17-day-old chick embryos were labeled with [35S]methionine in the presence of 0.3 mM alpha,alpha'-dipyridyl. The unhydroxylated, 35S-labeled pro alpha chains and alpha chains were isolated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. The pro alpha and alpha chains...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kao WW

    更新日期:1985-02-01 00:00:00

  • Transforming growth factor-beta stimulates collagen and fibronectin synthesis by human corneal stromal fibroblasts in vitro.

    abstract::The effects of transforming growth factor-beta (TGF beta) and epidermal growth factor (EGF) on the synthesis of collagen and fibronectin, and on the proliferation of human corneal stromal fibroblasts in vitro, were evaluated. Human corneal stromal fibroblasts in culture were incubated for 48 hours with TGF beta or EGF...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Ohji M,SundarRaj N,Thoft RA

    更新日期:1993-08-01 00:00:00

  • Aberrant Epigenetic Alterations of Glutathione-S-Transferase P1 in Age-Related Nuclear Cataract.

    abstract:PURPOSE:Oxidative damage of lens tissue contributes to the formation of age-related cataract. Pi-class glutathione-S-transferase (GSTP1) plays a role in the removal of oxidative adducts by transferring them to glutathione. To assess epigenetic regulation of GSTP1 and its potential role in age-related nuclear cataract (...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Chen J,Zhou J,Wu J,Zhang G,Kang L,Ben J,Wang Y,Qin B,Guan H

    更新日期:2017-03-01 00:00:00

  • Microcirculation in eyes after rhegmatogenous retinal detachment surgery.

    abstract:PURPOSE:To investigate the tissue blood flow in the neuroretinal rim of the optic disk and macula after rhegmatogenous retinal detachment (RRD) surgery. METHODS:Tissue blood flow in the neuroretinal rim of the optic disk and macula was measured with the Heidelberg retina flowmeter in 53 eyes of 53 patients who had und...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Sato EA,Shinoda K,Kimura I,Ohtake Y,Inoue M

    更新日期:2007-09-01 00:00:00

  • Efficacy of antiviral agents in feline herpetic keratitis: results of an in vitro study.

    abstract:PURPOSE:To determine, by a plaque reduction assay, the in vitro efficacy of novel antiviral agents in the treatment of feline herpes virus 1 (FHV-1) keratitis in the domestic cat (Felis felis). MATERIALS AND METHODS:A standard plaque reduction assay was performed using a laboratory strain of FHV-1 and embryo-derived f...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Williams DL,Fitzmaurice T,Lay L,Forster K,Hefford J,Budge C,Blackmore K,Robinson JC,Field HF

    更新日期:2004-08-01 00:00:00

  • Association of changes in intracellular cyclic AMP with changes in phagocytosis in cultured rat pigment epithelium.

    abstract::Papavarine, cholera toxin, and isoproterenol each elevated cyclic AMP in cultured rat retinal pigment epithelium and reduced the phagocytosis of isolated rod outer segments (38-66% of controls) during a 4 hour incubation. In addition, cultured pigment epithelium was preincubated with 25 mM exogenous cyclic AMP for two...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Edwards RB,Flaherty PM

    更新日期:1986-01-01 00:00:00

  • Prolonged exposure to high glucose impaired cellular behavior of normal human corneal epithelial cells.

    abstract:PURPOSE:To determine the effect of prolonged exposure to high glucose on cellular behavior of normal human corneal epithelial cells (HCEC). METHODS:HCEC were cultured in medium under normal or high glucose conditions for 14 days. Proliferation was evaluated by direct cell counting and [(3)H]thymidine incorporation. Ce...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Fujita H,Morita I,Takase H,Ohno-Matsui K,Mochizuki M

    更新日期:2003-10-01 00:00:00

  • Discrete expression and distribution pattern of TIMP-3 in the human retina and choroid.

    abstract:PURPOSE:Extracellular matrix homeostasis is dependent in part upon a family of matrix metalloproteinases and their specific inhibitors, the tissue inhibitors of metalloproteinases (TIMPs). Recently, gene defects in TIMP-3 have been identified in the affected individuals of several families with Sorsby's fundus dystroph...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Vranka JA,Johnson E,Zhu X,Shepardson A,Alexander JP,Bradley JM,Wirtz MK,Weleber RG,Klein ML,Acott TS

    更新日期:1997-02-01 00:00:00

  • Rabbits have a circadian rhythm of aqueous humor cyclic AMP.

    abstract::Rabbits have circadian rhythms of intraocular pressure (IOP) and aqueous flow. The dark phase increases of IOP and flow are associated with increased aqueous cyclic AMP. If the daily rhythm of aqueous cyclic AMP reflects changes of tissue levels of cyclic AMP which mediate one of the mechanisms which control the rhyth...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kiuchi Y,Gregory DS

    更新日期:1992-09-01 00:00:00

  • Calmodulin antagonists induce changes in lens permeability and transparency.

    abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Sanderson J,Gandolfi SA,Duncan G

    更新日期:1994-03-01 00:00:00

  • Open-angle Glaucoma and Sensorineural Hearing Impairment in the Korean Population.

    abstract:PURPOSE:This study identifies the prevalence and risk factors of sensorineural hearing loss and primary open-angle glaucoma (POAG). MATERIALS AND METHODS:Patients aged 19 years or older who had undergone both ophthalmologic examination and audiometry as part of the Korea National Health and Nutrition Examination Surve...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kim JM,Lee MY,Kim JW,Lee J,Kim HJ,Cha SC,Kim NR

    更新日期:2020-08-01 00:00:00

  • Strain specificity of spontaneous and adrenergically induced HSV-1 ocular reactivation in latently infected rabbits.

    abstract::Spontaneous ocular shedding and adrenergic induction of ocular shedding were examined in rabbits infected with ten strains of herpes simplex virus type 1 (HSV-1): McKrae, KOS, F, Rodanus, 17 Syn+, RE, E-43, SC-16, MacIntyre, and CGA-3. All ocular inoculations were with 50 microliter of HSV-1 with titers between 1-10 X...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Hill JM,Rayfield MA,Haruta Y

    更新日期:1987-01-01 00:00:00

  • Chronic electrical stimulation of sympathetic nerves: effects on blood-aqueous barrier.

    abstract::The effect of electrical stimulation of the sympathetic nerves on the blood-aqueous barrier was investigated in rabbits. The permeability of the barrier was assessed during either acute, chronic or following chronic nerve stimulation. During acute and chronic stimulation of the sympathetic nerves, fluorescein entered ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Bartels SP,Pawlowski AM

    更新日期:1990-10-01 00:00:00

  • Optimization of Cultured Human Corneal Endothelial Cell Sheet Transplantation and Post-Operative Sheet Evaluation in a Rabbit Model.

    abstract:PURPOSE:To optimize cultured human corneal endothelial cell (cHCEC) sheet transplantation technique for maintenance of cHCEC viability. MATERIALS AND METHODS:cHCEC sheets cultured on a collagen scaffold were covered with or without Viscoat® and exposed to humidified air in the incubator. cHCEC sheets with or without V...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Yamaguchi M,Shima N,Kimoto M,Ebihara N,Murakami A,Yamagami S

    更新日期:2016-09-01 00:00:00

  • Measurement of iris color using computerized image analysis.

    abstract:PURPOSE:To develop a standardized method for measuring from iris photographs, light and dark segments of areas and densities of iris color. METHODS:Computerized image analysis was used to measure the iris photographs. The reproducibility of this method was studied in 30 normal eyes with three different colored irides,...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Takamoto T,Schwartz B,Cantor LB,Hoop JS,Steffens T

    更新日期:2001-06-01 00:00:00