Abstract:
PURPOSE:To determine the effect of prolonged exposure to high glucose on cellular behavior of normal human corneal epithelial cells (HCEC). METHODS:HCEC were cultured in medium under normal or high glucose conditions for 14 days. Proliferation was evaluated by direct cell counting and [(3)H]thymidine incorporation. Cell cycle analysis was performed using flow cytometry. The ability of HCEC to attach to type I collagen was evaluated using a short-term colorimetric adhesion assay. The effect of high glucose on the expression of integrin alpha(3)beta(1) was also evaluated using flow cytometry. RESULTS:Cell number and [(3)H]thymidine incorporation under high glucose conditions decreased compared with those under normal glucose conditions. The cells exposed to high glucose were G(0)/G(1) than untreated cells. The adhesion ability of HCEC under high glucose conditions decreased compared to normal glucose conditions. Expression of integrin alpha( 3)beta(1) was down-regulated under high glucose conditions. CONCLUSIONS:High glucose had deleterious effects on cellular behavior of HCEC, which might cause delayed corneal epithelial wound healing in diabetic keratopathy.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Fujita H,Morita I,Takase H,Ohno-Matsui K,Mochizuki Mdoi
10.1076/ceyr.27.4.197.16598subject
Has Abstractpub_date
2003-10-01 00:00:00pages
197-203issue
4eissn
0271-3683issn
1460-2202journal_volume
27pub_type
杂志文章abstract::Recently, all the structural features of non-proliferative diabetic retinopathy have been demonstrated in mice fed 30% galactose for 21-26 months. To determine whether changes in retinal matrix protein mRNA levels occur early in the course of murine diabetes we used a competitive RT-PCR method to quantitate retinal mR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Intravitreal injection of purified human chorionic gonadotropin (hCG) in rabbits decreased intraocular pressure (IOP). A dose-dependent decrease in IOP was observed with intravitreal hCG concentrations at 30 nM and 100 nM. The onset of this effect was later than 10 hr following the injection and it lasted for more tha...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809015151
更新日期:1988-10-01 00:00:00
abstract::Lipid synthesis was investigated in bovine lens in organ culture for 9 hours using 14C acetate as a precursor. The greatest incorporation occurred in phospholipid fraction with phosphatidyl choline being the major phospholipid labeled. Considerable activity was incorporated in the free fatty acid fraction, cholesterol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034889
更新日期:1987-09-01 00:00:00
abstract:PURPOSE:To determine the effects of a subconjunctival injection of mitomycin-C (MMC) on iridial circulation in rabbits. METHODS:Dutch rabbits anesthetized with pentobarbital received a 0.2-ml subconjunctival injection of 0.4 mg/ml or 0.1 mg/ml MMC; the contralateral eye received 0.2 ml physiological saline. Intraocula...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.197.5327
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:To investigate the effects of rapamycin on the TNF-α-induced secretion of interleukin-6 (IL-6) and IL-8 in orbital fibroblasts and its possible mechanism. MATERIALS AND METHODS:Orbital fibroblasts were obtained from patients with thyroid-associated ophthalmopathy. IL-6 and IL-8 levels were measured by enzyme-l...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.750368
更新日期:2013-06-01 00:00:00
abstract::Papavarine, cholera toxin, and isoproterenol each elevated cyclic AMP in cultured rat retinal pigment epithelium and reduced the phagocytosis of isolated rod outer segments (38-66% of controls) during a 4 hour incubation. In addition, cultured pigment epithelium was preincubated with 25 mM exogenous cyclic AMP for two...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608995161
更新日期:1986-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003597230
更新日期:2010-05-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) are involved in posterior capsule opacification (PCO), but the mechanisms that promote MMP expression are yet to be determined. In this study, we investigated whether type I collagen, which is only detected in aged or cataractous lens capsules, affects the expression and activat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.853194
更新日期:2014-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.28.1.17.23488
更新日期:2004-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.2.133.5531
更新日期:2001-02-01 00:00:00
abstract:PURPOSE:Recent studies have shown that the levels of interleukin (IL)-1beta, IL-6 and IL-8 are associated with the severity of infectious diseases. The purpose of this study was to investigate whether IL-1beta regulates the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.6.406.6969
更新日期:2001-12-01 00:00:00
abstract::Purpose: Notch signaling plays a vital role in the differentiation and proliferation of corneal epithelial cells from limbal stem cells. The temporal regulation of Notch signaling during this differentiation remains unknown. Hence, we investigated the importance of temporal activation/blockage of Notch signaling durin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1673436
更新日期:2020-04-01 00:00:00
abstract::We previously reported that Ia+ monolayers of LEW rat corneal endothelial (CE) cells were unable to stimulate proliferation of MHC compatible T cell lines or IL-2 release from hybridomas, and inhibited [3H]-thymidine incorporation when added to conventional lymphocyte proliferation assays. Our purpose was to further a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999891
更新日期:1994-08-01 00:00:00
abstract:PURPOSE:Psychiatric conditions and not just the treatments themselves might be involved in the pathophysiology of dry eye disease (DED). The aim of our study was to evaluate the association between depression and DED using objective and subjective tests in patients with newly diagnosed depressive disorder who were not ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1236966
更新日期:2017-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1662056
更新日期:2020-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1387273
更新日期:2018-02-01 00:00:00
abstract::A method is described for the preparation of plasma membrane enriched fractions from bovine retinal pigment epithelium (RPE) by means of differential centrifugation followed by the use of self forming gradients of Percoll. A detailed analysis of the distribution of organelle specific markers (nuclei, mitochondria, lys...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000806
更新日期:1984-09-01 00:00:00
abstract::Membrane response to the various temperatures as one of the external factors was investigated in the lenses of the poikilothermal animal and the homothermal animal. The rainbow trout lens was used as the poikilothermal material and the rat lens as the homothermal material. The rainbow trout lens maintained in vitro it...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509025158
更新日期:1985-04-01 00:00:00
abstract::Two alternative methods for the study of the lens cytoskeleton are described which serve to overcome some of the difficulties imparted by the unique biology of the lens. The first technique involves rapid freezing, thick sectioning, and selective extraction and/or fixation of the lens section. This approach offers sev...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997582
更新日期:1990-11-01 00:00:00
abstract::Using an animal model of retinoblastoma in inbred rats and cultured human adenovirus type 12-induced retinoblastoma-like tumor cells (RAO 188), complement-dependent cytotoxicity was determined by measuring release of 3H-uridine labelled RNA. Sera from rats in which tumors did not grow after adenovirus type 12 inoculat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019973
更新日期:1981-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1606252
更新日期:2019-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309029227
更新日期:1993-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1789664
更新日期:2021-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.9.924.5133
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:To explore the in vitro proliferative potential of human limbal epithelial cells after 31 degrees C organ-culture storage and to investigate putative factors influencing it. METHODS:185 cultures of limbal explants were carried-out either from full-thickness explants (n = 102) or from enzymatically dissociated ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600681228
更新日期:2006-05-01 00:00:00
abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001869
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:Unoprostone isopropyl is an ocular hypotensive that was originally produced as a prostaglandin F2alpha analogue and is eventually recognized as a synthetic docosanoid. The compound is recently suggested to have potent neuroprotective ability in the retina. The purpose of this study is to test whether and how th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490889465
更新日期:2004-12-01 00:00:00
abstract:PURPOSE:Endothelial cells of different vascular systems may express site-specific adhesion molecules to attract leukocyte subsets. This study describes a method to visualize and compare leukocyte-endothelial interactions in three vascular beds within the same eye in mice. METHODS:Digital in vivo fluorescence microscop...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.3.214.8303
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:To investigate the agreement of angular locations of retinal nerve fiber layer (RNFL) defect margins in glaucomatous eyes by using red-free fundus photographs and Cirrus high-definition optical coherence tomography (OCT) RNFL deviation and thickness maps. METHODS:We examined 380 RNFL defects that showed clear ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.900805
更新日期:2014-11-01 00:00:00
abstract::The acyclic nucleoside phosphonate analogue PMEA [9-(2-phosphonylmethoxyethyl)adenine] is a broad spectrum antiviral agent effective against DNA viruses and retroviruses. It is particularly active against the human immunodeficiency virus and, like other phosphonylmethoxyalkyl derivatives, it also inhibits HSV-1, TK- H...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020370
更新日期:1991-01-01 00:00:00