Abstract:
:We previously reported that Ia+ monolayers of LEW rat corneal endothelial (CE) cells were unable to stimulate proliferation of MHC compatible T cell lines or IL-2 release from hybridomas, and inhibited [3H]-thymidine incorporation when added to conventional lymphocyte proliferation assays. Our purpose was to further analyze the mechanism of the inhibitory activity of CE cells on T lymphocyte activation. Mitogen-induced proliferative responses of splenocytes were found to be as susceptible to inhibition by CE cells as previously reported for antigen-specific activation of T cell lines. Antigen presenting cell (APC) antigen pulsing experiments showed that CE cells did not inhibit antigen processing. Flow cytometry and microscopic observation of the co-cultures revealed that T cells became activated in the presence of antigen, APC and CE cells, exhibiting morphologic changes of blast cell formation, although they did not divide unless given exogenous IL-2. However, if T cells were preactivated in the absence of CE cells, they were no longer susceptible to inhibition if subsequently transferred into CE cell-conditioned medium or onto CE cells. Evidence for an inhibitory factor in CE cell culture supernatant was revealed by two approaches: 1) addition of conditioned medium from CE cell cultures led to inhibition of lymphocyte proliferation assays, and 2) split-well assays also demonstrated the existence of a cell-free immunosuppressive factor produced by the CE cells. However, the inhibition mediated by supernatant alone was less potent than that by direct T cell contact with CE cells, implying that cell-cell interaction contributed to the inhibition. Indomethacin, a prostaglandin synthetase inhibitor, did not reverse CE cell-mediated inhibition. Neutralizing antibodies to TGF-beta 1 and 2 did not reverse the inhibition by CE cells. In summary, T cells received activation signals from APC in the presence of CE cells, but proliferation was inhibited unless exogenous lymphokine was added.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Kawashima H,Gregerson DSdoi
10.3109/02713689408999891subject
Has Abstractpub_date
1994-08-01 00:00:00pages
575-85issue
8eissn
0271-3683issn
1460-2202journal_volume
13pub_type
杂志文章abstract:PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells ar...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract::Blood and lymphatic vessel proliferation into the cornea occurs after severe trauma. Whilst the events early in blood vessel growth are reasonably understood, little is known of the early events in lymphangiogenesis. In this study, a model for proliferating lymphatic vessels using tritiated-thymidine autoradiography a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013898
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To evaluate differences of phagocytic capacities in rat retinal pigment epithelial (RPE) cells, iris pigment epithelial (IPE) cells, and basic fibroblast growth factor (bFGF) cDNA-transfected IPE (bFGF-IPE) cells in vitro. METHODS:The RPE cells and IPE cells were isolated from adult Long Evans rats' eyes. The ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.3.185.5465
更新日期:2001-09-01 00:00:00
abstract:PURPOSE:There is a substantial literature showing that the selective estrogen receptor modulator tamoxifen can block swelling-activated chloride channels, such as those in astrocytes. This study aimed to test the hypothesis that anatomic characteristics consistent with swelling may be measured within the optic nerve he...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600602547
更新日期:2006-04-01 00:00:00
abstract::Purpose: To evaluate the effect of isolated oblique muscle weakening on horizontal deviation in patients with A- and V-pattern exotropia.Methods: The medical records of patients who underwent bilateral oblique muscle weakening for A- and V-pattern exotropia associated oblique muscle overaction between 2011 and 2018 we...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1660795
更新日期:2020-02-01 00:00:00
abstract:PURPOSE:To determine by immunohistochemistry the distribution of different nitric oxide synthases (NOS) isoforms in the porcine eye. METHODS:By light microscopy (immunofluorescence), porcine ocular tissues were investigated using monoclonal antibodies against neuronal NOS (nNOS; NOS I), endothelial NOS (ecNOS; NOS III...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.5.375.5355
更新日期:1999-05-01 00:00:00
abstract::Free radicals and oxidative damage play roles in aging and age-related ocular diseases such as cataracts, so defensive mechanisms become important factors for protection. Because N-acetylation is involved in a wide variety of detoxification processes, this study was conducted to examine the relationship between the ac...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680591003799
更新日期:2005-10-01 00:00:00
abstract:PURPOSE:To investigate the effects of pyrrolidine dithiocarbamate (PDTC), a nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) inhibitor, on the expression of matrix metalloproteinases (MMP) and MMP-mediated apoptosis in retinal ganglion cells (RGCs) following the transection of the optic nerve (ON) in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.606591
更新日期:2011-11-01 00:00:00
abstract:PURPOSE:To evaluate transcorneal penetration of topically applied 0.5% levofloxacin into the aqueous humor in human eyes. METHODS:Twenty cataract patients (14 females, 6 males) received 3 drops of 0.5% levofloxacin at 15 min intervals from 90 minutes before the surgery. At the beginning of the cataract surgery, 50 mic...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.5.403.8517
更新日期:2002-05-01 00:00:00
abstract::Although experimental models of autoimmune uveitis predict pinealitis coincident with uveitis, there is no direct evidence of pineal pathology accompanying a human uveitis. Horses with naturally occurring uveitis are a potential source of eye and pineal tissues that are not available from human patients with active uv...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689208999525
更新日期:1992-01-01 00:00:00
abstract:AIMS:To elucidate the intramuscular distribution and branching patterns of the abducens nerve in the lateral rectus (LR) muscle so as to provide anatomical confirmation of the presence of compartmentalization, including for use in clinical applications such as botulinum toxin injections. METHODS:Thirty whole-mount hum...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1438631
更新日期:2018-06-01 00:00:00
abstract:PURPOSE:We wished to establish which matrix metalloproteinases (MMPs) and metalloproteinase inhibitors (TIMPs) were present in human interphotoreceptor matrix (IPM) and vitreous. METHODS:IPM and vitreous were obtained from postmortem human eyebank eyes. Western immunoblots were probed with antibodies against human MMP...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.2.132.5610
更新日期:1998-02-01 00:00:00
abstract::From knowledge of the optical parameters of experimental animal eyes and radiometric parameters of the light source, it is possible to provide accurate retinal irradiances (dose rates). Examples are given for the rat, cat, monkey and other animals of interest. Fluorescent lamps and incandescent lamp radiances are prov...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688408997199
更新日期:1984-01-01 00:00:00
abstract::Cornea endothelial cells sensitized with rose bengal and exposed to light demonstrated a wavelength dependent alteration in physiological response. Corneas exposed to 549 millimicron swelled at rates similar to controls exposed to identical energy levels of incandescent light. Corneas exposed to 451, 500, 612, and 651...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019990
更新日期:1981-01-01 00:00:00
abstract::High pressure liquid chromatography was used to measure alpha-tocopherol in the retinas of rats reared in a cyclic light or dark environment. These measurements were performed on extracts of whole retinas during the developmental period, 18-60 days, and on isolated ROS from adult animals. Similar alpha-tocopherol dete...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409007414
更新日期:1984-11-01 00:00:00
abstract:PURPOSE:RPE65, a retinal pigment epithelium-specific 65-kDa protein, plays a critical role in the visual cycle of the eye. Rpe65(-/-) mice develop vision loss due to a lack of 11-cis-retinal, degradation of M-opsin and mislocalization of S-opsin. Several studies have suggested that 9-cis-β-carotene, a precursor of 9-ci...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.907430
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:The similar and overlapping activity of VEGF and the potent corneal-derived angiogenic eicosanoid 12(R)-HETrE calls for a study of the temporal relationship in the expression of these two autocoids. Since recent evidence suggests that hypoxia induces the expression of a CYP4B1 mRNA which might be involved in th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.1.1.5422
更新日期:2001-07-01 00:00:00
abstract::In these experiments we characterize rabbit and bovine corneal endothelia cell cultured on microporous membrane filters (0.6cm2). Cell cultured bovine or rabbit corneal endothelial cells (subcultures 1-3) were seeded onto Millicell-HA filter inserts. Electrical resistance measured across the cultured monolayers increa...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209069168
更新日期:1992-01-01 00:00:00
abstract::An image analyser installed in a 16 bit personal computer was used to measure grey levels in red free photographs of normal and glaucomatous eyes. Regional photographic image intensity and density were correlated with Humphrey Field Analyser results for corresponding areas of visual field. Two methods for standardisin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033210
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:To compare the differences in higher-order aberrations (HOAs) after laser subepithelial keratomileusis (LASEK) between two different laser platforms. METHODS:One hundred and seven eyes of 107 patients were included in this study. Fifty-six eyes underwent LASEK with the Triple-A profile (an ablation profile of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1726404
更新日期:2020-09-01 00:00:00
abstract:AIM:To test the feasibility of implanting human anterior lens capsules (HALCs) with porcine corneal endothelial cells (pCEC) in vivo in Göttingen minipigs and at the same time test the suitability of Göttingen minipig as model for endothelial keratoplasty. MATERIALS AND METHODS:Cell-carrier constructs of decellularize...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1706747
更新日期:2020-08-01 00:00:00
abstract:PURPOSE:To evaluate clinical outcome(s) and complication(s) of topical bevacizumab (avastin) in patients with impending recurrent pterygium. PATIENTS AND METHODS:This prospective clinical trial included 54 consecutive patients (54 eyes), who underwent pterygium surgery with bare sclera and mitomycin, and who were diag...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.3109/02713680903395273
更新日期:2010-01-01 00:00:00
abstract::Goggles that degrade the retinal image produce axial enlargement of the ocular globe and large myopic refractive errors. Many authors have assumed that visual image degradation itself leads to myopia. Hodos and co-authors have shown, however, that goggled eyes in chicks are considerably warmer than normal. Such temper...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999467
更新日期:1993-03-01 00:00:00
abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408995780
更新日期:1994-03-01 00:00:00
abstract::Two proteins, with molecular weights of 49 (CP49) and 115 kDa (CP115) as judged by SDS PAGE, have been shown by immunocytochemistry to be components of the beaded filament, a cytoskeletal structure thus far demonstrated only in the lens fiber cell. We have used antibodies reactive with CP49 to screen a mouse lens cDNA...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999499
更新日期:1993-01-01 00:00:00
abstract::Purpose/aim of the study: To observe and analyze the weekly changes in morphology and size of vacuoles, so called Wedl cells, in eyes with posterior subcapsular cataract (PSC). MATERIALS AND METHODS:Thirty-six eyes of 36 patients with pronounced PSC were included in this prospective study. High-resolution retroillumi...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1343852
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:Rhodopsin is a zinc-binding protein. We investigated the effect of low concentrations of zinc on the initial phosphorylation of rhodopsin. METHODS:Dark-adapted bovine rod outer segments (ROS) were incubated with (gamma 32P) ATP and 5 mM magnesium in the presence and absence of micromolar amounts of zinc. The R...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017650
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:To examine the association between macular pigment optical density (MPOD) and rod-mediated dark adaptation (RMDA) in persons ≥60 years old with normal maculas as determined by an accepted color fundus photography grading system. METHODS:This cross-sectional analysis used baseline data from eyes in the Alabama ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1460380
更新日期:2018-07-01 00:00:00
abstract::Importance: To analyze the characteristics and risk factors for pupillary capture (PC) of the intraocular lens (IOL) in congenital ectopia lentis (CEL) patients.Methods: Data of CEL patients who underwent lens extraction and intraocular implantation from 2013 to 2016 at Zhongshan Ophthalmic Center in China were collec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1668418
更新日期:2020-04-01 00:00:00
abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514055
更新日期:2018-12-01 00:00:00