Peroxynitrite-induced apoptosis in photoreceptor cells.

Abstract:

PURPOSE:To study the mechanisms of peroxynitrite-induced photoreceptor cell damage, using retinal cultures and a peroxynitrite donor, 3-morpholinosydnonimine (SIN-1). METHODS:Retinal explants obtained from 20-day-old Lewis rat pups, were exposed to SIN-1 for varying lengths of time at varying concentrations. Apoptosis in the photoreceptor cells was detected using the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method and a DNA fragmentation assay. Selected retinal samples were processed for an ultrastructural analysis to confirm apoptosis. The retinas exposed to SIN-1 were tested for the expression of caspase-3 by immunohistochemistry and a Western blot analysis. The retinas were also evaluated for the prevention of apoptosis in the presence of the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk). RESULTS:The retinal explants exposed to SIN-1 showed a significant increase in the presence of TUNEL-positive photoreceptor cells. Similarly lineal increases in TUNEL-positive cells were seen in the presence of increasing concentrations of SIN-1. DNA ladder formation was seen with the exposure of SIN-1. Ultrastructurally, SIN-1 exposed retinas revealed typical apoptotic changes in the photoreceptor cell nuclei. The retinas preincubated with urate for 6 hours and exposed to SIN-1 for 16 hours showed significantly fewer TUNEL-positive cells compared to the retinas exposed to SIN-1 alone (p < 0.05). Moreover, the retinas exposed to SIN-1 showed the expression of caspase-3. This expression, as well as the number of apoptotic photoreceptors, significantly decreased in the presence of Z-VAD-fmk. CONCLUSIONS:These results show that peroxynitrite induces apoptosis in photoreceptor cells and that such retinal damage appears to be mediated by caspase-3. The apoptotic process can be minimized by peroxynitrite scavenger urate, as well as by the caspase inhibitor Z-VAD-fmk.

journal_name

Curr Eye Res

journal_title

Current eye research

authors

Ito S,Wu GS,Kimoto T,Hisatomi T,Ishibashi T,Rao NA

doi

10.1076/ceyr.28.1.17.23488

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

17-24

issue

1

eissn

0271-3683

issn

1460-2202

pii

Q2LW9740PGUM4JHC

journal_volume

28

pub_type

杂志文章
  • Pinealitis coincident with recurrent uveitis: immunohistochemical studies.

    abstract::Although experimental models of autoimmune uveitis predict pinealitis coincident with uveitis, there is no direct evidence of pineal pathology accompanying a human uveitis. Horses with naturally occurring uveitis are a potential source of eye and pineal tissues that are not available from human patients with active uv...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689208999525

    authors: Kalsow CM,Dwyer AE,Smith AW,Nifong TP

    更新日期:1992-01-01 00:00:00

  • The cross-species A3 adenosine-receptor antagonist MRS 1292 inhibits adenosine-triggered human nonpigmented ciliary epithelial cell fluid release and reduces mouse intraocular pressure.

    abstract:PURPOSE:Antagonists to A3 adenosine receptors (ARs) lower mouse intraocular pressure (IOP), but extension to humans is limited by species variability. We tested whether the specific A3AR antagonist MRS 1292, designed to cross species, mimicks the effects of other A3AR antagonists on cultured human nonpigmented ciliary ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713680590953147

    authors: Yang H,Avila MY,Peterson-Yantorno K,Coca-Prados M,Stone RA,Jacobson KA,Civan MM

    更新日期:2005-09-01 00:00:00

  • Cell turnover in ciliary epithelium compared to other slow renewing epithelia in the adult mouse.

    abstract::Adult albino mice received tritiated thymidine either injected subcutaneously (333 microCi) every 6 hours for up to 36 hours, or administered in their drinking water for up to 74 days (about 45 microCi/day). Tissue sections were examined with autoradiography to determine the distribution of the label. Animals receivin...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688809047029

    authors: McDonald TF,Green K

    更新日期:1988-03-01 00:00:00

  • Immediate Effect of 3% Diquafosol Ophthalmic Solution on Tear MUC5AC Concentration and Corneal Wetting Ability in Normal and Experimental Keratoconjunctivitis Sicca Rat Models.

    abstract:PURPOSE:To evaluate the immediate effect of 3% diquafosol ophthalmic solution on tear MUC5AC concentration, periodic acid-Schiff (PAS)-positive goblet cells, and tear film stability in normal and keratoconjunctivitis sicca (KCS) rat models. METHODS:Rats were divided into normal and KCS groups. 3% of diquafosol solutio...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2016.1233986

    authors: Choi KE,Song JS,Kang B,Eom Y,Kim HM

    更新日期:2017-05-01 00:00:00

  • Pancreatic islet transplantation ameliorates the effect of diabetes on retinal capillary endothelial cell kinetics.

    abstract::Perturbations in capillary endothelial cell function are early events in the pathogenesis of diabetic retinopathy. We used tritiated thymidine autoradiography to study the turn-over rate of retinal capillary endothelial cells in retinovascular preparations from normal, untreated diabetic and diabetic mice treated with...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688909025802

    authors: Cuthbertson RA,Mandel TE

    更新日期:1989-07-01 00:00:00

  • Quantitative Analysis of Optical Coherence Tomography Angiography Features in Patients with Nonocular Behcet's Disease.

    abstract:PURPOSE:To quantitatively measure the flow, nonflow, density, and foveal avascular zone (FAZ) assessment tool parameters of optical coherence tomography angiography (OCTA) in patients with nonocular Behcet's Disease (BD), and to compare the results with those obtained in healthy controls. METHODS:FAZ parameters, flow ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2018.1530361

    authors: Goker YS,Yılmaz S,Kızıltoprak H,Tekin K,Demir G

    更新日期:2019-02-01 00:00:00

  • Aging alters the phagocytic capability of inflammatory cells induced into cornea.

    abstract::The changes in mouse corneal inflammatory cell population and the phagocytic capability of these cells were studied quantitatively at 24 and 48 hours after eliciting inflammatory cells into the cornea by Pseudomonas aeruginosa inoculation. Mice were selected for study according to their ability (young adult Swiss-Webs...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689008995199

    authors: Hazlett LD,Kreindler FB,Berk RS,Barrett R

    更新日期:1990-02-01 00:00:00

  • Age does not affect contractile responses of the isolated rhesus monkey ciliary muscle to muscarinic agonists.

    abstract::In primates, ciliary muscle contraction causes accommodation and facilitates aqueous outflow. In living rhesus monkeys, accommodative, outflow facility, and ciliary muscle movement responses to cholinergic agonists all decline with age. We developed an apparatus to determine in vitro whether the latter is related to i...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689309024623

    authors: Poyer JF,Kaufman PL,Flügel C

    更新日期:1993-05-01 00:00:00

  • Protective effect of passive immunization on herpetic retinitis of newborn rabbits.

    abstract::We studied the protective effects of passive immunization with virus specific antibody in newborn rabbits inoculated subcutaneously with type 2 herpes simplex virus (HSV-2). Newborn rabbits given anti-HSV-2 antibody intraperitoneally (IP) on days 0, 2 and 4 post infection had smaller herpetic skin lesions and reduced ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688709020084

    authors: Friedlaender RP,Oh JO,Minasi P,Ohashi Y

    更新日期:1987-01-01 00:00:00

  • Computerised densitometry of red-free retinal photographs correlated with automatic perimetry.

    abstract::An image analyser installed in a 16 bit personal computer was used to measure grey levels in red free photographs of normal and glaucomatous eyes. Regional photographic image intensity and density were correlated with Humphrey Field Analyser results for corresponding areas of visual field. Two methods for standardisin...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688809033210

    authors: Cooper RL,Eikelboom RH,Barry C

    更新日期:1988-08-01 00:00:00

  • A Comparison of different scoring terminations rules for visual acuity testing: from a computer simulation to a clinical study.

    abstract::Purpose: To compare four visual acuity (VA) scoring termination rules. Methods: A computer simulation generated 30,000 virtual patients who underwent 10 repetitions for each of four termination rules, on both the Snellen and ETDRS charts (2.4 million tests performed in total). Three termination rules focused on the sm...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2019.1589524

    authors: Mimouni M,Shamir RR,Cohen AD,El-Yaniv R,Cohen MJ,Joskowicz L,Blumenthal EZ

    更新日期:2019-07-01 00:00:00

  • The effect of postnatal growth retardation on abnormal neovascularization in the oxygen exposed neonatal rat.

    abstract::Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newb...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689608995831

    authors: Holmes JM,Duffner LA

    更新日期:1996-04-01 00:00:00

  • Efficacy of the Inverted Internal Limiting Membrane Flap Technique with Perfluorocarbon Liquid-Mediated Selective Staining for Large Macular Hole Repair.

    abstract:PURPOSE:The aim of the present study was to evaluate the efficacy of the inverted internal limiting membrane (ILM) flap technique that involved the use of a perfluorocarbon liquid (PFCL) bubble for the selective prevention of indocyanine green (ICG) staining of the parts of the inverted flap in contact with the retinal...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2018.1524014

    authors: Ra H,Lee WK

    更新日期:2019-01-01 00:00:00

  • 24-hour intraocular pressure and blood pressure levels with latanoprost/timolol fixed combination versus timolol.

    abstract:PURPOSE:To evaluate 24-hr intraocular pressure (IOP) and blood pressure (BP) with timolol or latanoprost/timolol fixed combination (LTFC). METHODS:Patients with primary open-angle glaucoma or ocular hypertension with normal blood pressure were randomized to LTFC, dosed each evening, or timolol dosed twice daily in a c...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验

    doi:10.1080/02713680902850075

    authors: Konstas AG,Pikilidou MI,Tsironi S,Mikropoulos D,Kozobolis VP,Sarafidis PA,Lasaridis AN,Nelson LA,Stewart WC

    更新日期:2009-05-01 00:00:00

  • The Association of Nailfold Capillaroscopy with Systemic Matrix Metalloproteinase-9 Concentration in Normal-Tension Glaucoma.

    abstract:PURPOSE:To investigate the association of nailfold capillaroscopy, heart rate variability (HRV), and clinical characteristics of glaucoma with the plasma matrix metalloproteinase-9 (MMP-9) level in normal-tension glaucoma (NTG). MATERIALS AND METHODS:We conducted a prospective, cross-sectional study on 25 patients wit...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713683.2014.971937

    authors: Lee NY,Park HY,Park SH,Park CK

    更新日期:2015-01-01 00:00:00

  • Down-regulation of matrix metalloproteinase-9 by pyrrolidine dithiocarbamate prevented retinal ganglion cell death after transection of optic nerve in rats.

    abstract:PURPOSE:To investigate the effects of pyrrolidine dithiocarbamate (PDTC), a nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) inhibitor, on the expression of matrix metalloproteinases (MMP) and MMP-mediated apoptosis in retinal ganglion cells (RGCs) following the transection of the optic nerve (ON) in ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713683.2011.606591

    authors: Sun MH,Chen KJ,Tsao YP,Kao LY,Han WH,Lin KK,Pang JH

    更新日期:2011-11-01 00:00:00

  • 20:5n-3 but not 22:6n-3 is a preferred substrate for synthesis of n-3 very-long- chain fatty acids (C24-C36) in retina.

    abstract::The objective of this study was to determine if 20:5n-3 or 22:6n-3 is the primary precursor of very-long-chain fatty acids (VLCFAs; C24-C36) synthesized in retina. Rats were fed semisynthetic, nutritionally complete diet containing 20% (w/w) fat with 3% (w/w) of 22:6n-3. After 6 weeks feeding, the vitreal fluid of eac...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713680500246957

    authors: Suh M,Clandinin MT

    更新日期:2005-11-01 00:00:00

  • Renin mRNA is synthesized locally in rat ocular tissues.

    abstract::Components of the Renin Angiotensin System (RAS) have been detected in ocular tissues and fluids. The source of the ocular RAS proteins is unknown but possibilities include diffusion or leakage from the systemic circulation, specific uptake from the blood, or local synthesis. We have used RT-PCR and in situ hybridizat...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689409047011

    authors: Brandt CR,Pumfery AM,Micales B,Bindley CD,Lyons GE,Sramek SJ,Wallow IH

    更新日期:1994-10-01 00:00:00

  • Hydrogels as potential probes for investigating the mechanism of lenticular presbyopia.

    abstract:PURPOSE:To synthesize and characterize hydrogels with viscoelastic properties comparable to those of the natural lens. METHODS:Hydrogels were synthesized in water by free-radical polymerization of the monomer poly(ethyleneglycol)-monomethacrylate. Three different molecular weights of poly(ethyleneglycol)-dimethacrylat...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.22.5.384.5493

    authors: Murthy SK,Ravi N

    更新日期:2001-05-01 00:00:00

  • Evolution of TUNEL-labeling in the rat lens after in vivo exposure to just above threshold dose UVB.

    abstract:PURPOSE/AIM:To quantitatively analyse the evolution of TUNEL-labeling, after in vivo exposure to UVB. METHODS:Altogether, 16 Sprague Dawley rats were unilaterally exposed in vivo for 15 min to close to threshold dose, 5 kJ/m(2), of ultraviolet radiation in the 300 nm wavelength region. Animals were sacrificed in group...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713683.2013.783079

    authors: Kronschläger M,Yu Z,Talebizadeh N,Meyer LM,Hallböök F,Söderberg PG

    更新日期:2013-08-01 00:00:00

  • Serum and Aqueous Humor Levels of Fetuin-A in Pseudoexfoliation Syndrome.

    abstract:PURPOSE:To evaluate serum and aqueous humor levels of fetuin-A in patients with pseudoexfoliation syndrome (PEXS) in comparison with those of age- and sex-matched healthy subjects. MATERIALS AND METHODS:This prospective study included 25 patients with PEXS and 25 control subjects who were undergoing cataract surgery w...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2017.1324629

    authors: Yuksel N,Takmaz T,Ozel Turkcu U,Ergin M,Altinkaynak H,Bilgihan A

    更新日期:2017-10-01 00:00:00

  • Safety and efficacy of loteprednol etabonate for treatment of papillae in contact lens-associated giant papillary conjunctivitis.

    abstract::Loteprednol etabonate (LE) is a new corticosteroid based on the "soft drug" concept. Contact lens-associated giant papillary conjunctivitis (GPC) was studied as a model for the anti-inflammatory effect of LE. Patients with bilateral GPC were enrolled in a multicenter, randomized, double-masked, placebo-controlled, par...

    journal_title:Current eye research

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.3109/02713689308999455

    authors: Bartlett JD,Howes JF,Ghormley NR,Amos JF,Laibovitz R,Horwitz B

    更新日期:1993-04-01 00:00:00

  • Zinc causes an apparent increase in rhodopsin phosphorylation.

    abstract:PURPOSE:Rhodopsin is a zinc-binding protein. We investigated the effect of low concentrations of zinc on the initial phosphorylation of rhodopsin. METHODS:Dark-adapted bovine rod outer segments (ROS) were incubated with (gamma 32P) ATP and 5 mM magnesium in the presence and absence of micromolar amounts of zinc. The R...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689609017650

    authors: Shuster TA,Martin F,Nagy AK

    更新日期:1996-10-01 00:00:00

  • A Novel Approach to Analyze the Progression of Measured Corneal Sub-Basal Nerve Fiber Length in Continuously Expanding Mosaic Images.

    abstract::Purpose/Aim of the study: A recently proposed technique enables the generation of continuously increasing mosaic images of the corneal sub-basal nerve plexus (SNP) using in vivo corneal confocal microscopy (CCM). The aim of the present study was to investigate the progression of the corneal nerve fiber length (CNFL) m...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2016.1221977

    authors: Allgeier S,Winter K,Bretthauer G,Guthoff RF,Peschel S,Reichert KM,Stachs O,Köhler B

    更新日期:2017-04-01 00:00:00

  • Corneal thickness and volume measurements by swept source anterior segment optical coherence tomography in normal subjects.

    abstract:PURPOSE:To evaluate central corneal thickness (CCT) and corneal volume (CV) in healthy eyes using swept source anterior segment optical coherence tomography (SS-AS-OCT) and other devices. METHODS:Thirty-three healthy right eyes of 33 subjects were investigated. CCT was measured with SS-AS-OCT, rotating Scheimpflug cam...

    journal_title:Current eye research

    pub_type: 临床试验,杂志文章

    doi:10.3109/02713683.2012.745878

    authors: Fukuda R,Usui T,Miyai T,Mori Y,Miyata K,Amano S

    更新日期:2013-05-01 00:00:00

  • Lipid peroxidation in porcine irises: dependence on pigmentation.

    abstract:PURPOSE:Pigmentation of the iris is caused by varying amounts of melanin pigment granula in a constant number of melanocytes in the superficial stroma. Melanin has been shown to act as antioxidant. We have now investigated lipid peroxidation in dependence on stromal pigmentation in isolated porcine irises. METHODS:The...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.22.3.229.5511

    authors: Nau-Staudt K,Nau WM,Haefliger IO,Flammer J

    更新日期:2001-03-01 00:00:00

  • Effect of repeated applanation tonometry on the accuracy of intraocular pressure measurements.

    abstract:PURPOSE:To evaluate the stability of successive applanation tonometry measurements in glaucomatous eyes. METHODS:A prospective, comparative, randomized, and evaluator-masked study was conducted in a tertiary medical center. Sixty-seven patients with glaucoma attending our glaucoma clinic underwent four successive intr...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/02713681003678824

    authors: Gaton DD,Ehrenberg M,Lusky M,Wussuki-Lior O,Dotan G,Weinberger D,Snir M

    更新日期:2010-06-01 00:00:00

  • Rose bengal induced corneal swelling: relation to inciting wavelength.

    abstract::Cornea endothelial cells sensitized with rose bengal and exposed to light demonstrated a wavelength dependent alteration in physiological response. Corneas exposed to 549 millimicron swelled at rates similar to controls exposed to identical energy levels of incandescent light. Corneas exposed to 451, 500, 612, and 651...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688109019990

    authors: Hull DS,Csukas S,Green K

    更新日期:1981-01-01 00:00:00

  • Changes in connexin43 in early ocular surface development.

    abstract:PURPOSE:In the limbo-corneal epithelium the stem and early precursor epithelial cell pool is confined to the limbal rim. Among the features associated with this spatial segregation is the general paucity of connexin43 (Cx43) within the limbal basal cell population and its complete absence in resident stem cells. The li...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.24.6.430.8599

    authors: Wolosin JM,Schütte M,Zieske JD,Budak MT

    更新日期:2002-06-01 00:00:00

  • Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections.

    abstract::The new HERPCHEK (Dupont, No. Billerica, MA) enzyme immunosorbent assay (EIA) was used in a double-blind clinical study for rapid and specific detection of ocular herpes simplex virus (HSV) infection. This 4-hour assay can be used to demonstrate conclusively the presence of HSV antigen without culture and thereby rapi...

    journal_title:Current eye research

    pub_type: 临床试验,杂志文章

    doi:10.3109/02713688809033194

    authors: Dunkel EC,Pavan-Langston D,Fitzpatrick K,Cukor G

    更新日期:1988-07-01 00:00:00