Abstract:
:Purpose: To investigate the cytotoxic effects of fresh black carbon (FBC) particles and ozone-oxidized BC (OBC) particles on human corneal epithelial cells (HCECs), and the role of the NLRP3 inflammasome signal pathway in the process.Methods: HCECs were treated with different doses of FBC and OBC particles, the cell inhibitory effect was observed by MTS assay and the inhibitory concentration 50 (IC50) was calculated; the effect of FBC and OBC particles on cell apoptosis was assessed using flow cytometry-based annexin V/7AAD assay; the mRNA levels of NLRP3 inflammasome signal pathway components (NLRP3, ASC, and Caspase-1) and downstream cytokine (IL-1 β) were detected in HCECs.Results: After treatment for 24 h, the IC50 of FBC and OBC particles in HCECs were 295.5 μg/ml and 224.6 μg/ml, respectively. The percentages of cells in total apoptosis after exposure to FBC and OBC were significantly higher than in untreated control cells (P < .001). Moreover, OBC exposed cells exhibited significantly more total apoptosis than FBC exposed cells (P < .001). Compared to the untreated control, there was enhanced mRNA expression of NLRP3, Caspased-1, ASC, and IL-1β in both FBC and OBC treated HCECs (all P < .01). In addition, OBC treated HCECs yielded higher levels of IL-1β mRNA compared to FBC treated cells (P < .05).Conclusion: Both FBC and OBC particles have a cytotoxic effect on HCECs, accompanied by NLRP3 inflammasome activation, indicating that the NLRP3 inflammasome signaling pathway may be involved in the pathogenesis of the BC cytotoxic process. OBC is more toxic than FBC particles.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Long Q,Huang Y,Shang J,Liu Y,Chen Cdoi
10.1080/02713683.2019.1698051subject
Has Abstractpub_date
2020-06-01 00:00:00pages
680-685issue
6eissn
0271-3683issn
1460-2202journal_volume
45pub_type
杂志文章abstract::Calcium permeability was significantly increased in lenses from rats 36 h following a single injection of a cataractogenic dose of sodium selenite (30 nmol/g body weight) and this permeability returned to control values by 72 h post-injection (PI). The greater Ca2+ permeability could be partially reversed by incubatin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689308999466
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abstract::In an attempt to determine the factors involved in ocular pigment variation, the eyes of pallid mice (c57BL/6J-Pa/Pa), and those of littermate black mice (C57BL/6J-+/Pa) were examined ultrastructurally, histochemically, and biochemically. As controls, the eyes of black and congenic albino mice (C57BL/6J +/C2J and -C2J...
journal_title:Current eye research
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doi:10.3109/02713688208998376
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journal_title:Current eye research
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doi:10.1076/ceyr.22.2.133.5531
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abstract::We studied the protective effects of passive immunization with virus specific antibody in newborn rabbits inoculated subcutaneously with type 2 herpes simplex virus (HSV-2). Newborn rabbits given anti-HSV-2 antibody intraperitoneally (IP) on days 0, 2 and 4 post infection had smaller herpetic skin lesions and reduced ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709020084
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.5.432.5291
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abstract::Sixteen adult New Zealand white rabbits with previously confirmed herpes simplex virus type 1 (HSV-1) infections were stimulated by iontophoresis of 6-hydroxydopamine into the cornea and were followed-up by topical epinephrine treatment to confirm latency. A total of 224 ocular cultures were obtained, of which 73 were...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709044511
更新日期:1987-12-01 00:00:00
abstract::This study was conducted to determine the therapeutic efficacy of 3.0 mg/ml ciprofloxacin administered concurrently with one of two salts of prednisolone for the treatment of experimental pseudomonal keratitis. Rabbit corneas were injected intrastromally with Pseudomonas aeruginosa ATCC strain 27853. Sixteen hr after ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309024629
更新日期:1993-05-01 00:00:00
abstract:PURPOSE:Due to a possible link in humans between atherosclerosis, a high-fat diet, and the development of age-related retinal degenerations, we investigated retinal changes with age and diet in high-fat-fed C57BL/6 mice, a mouse model of human atherosclerosis. METHODS:We fed C57BL/6J mice either a normal chow diet or ...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:To determine the effects of a subconjunctival injection of mitomycin-C (MMC) on iridial circulation in rabbits. METHODS:Dutch rabbits anesthetized with pentobarbital received a 0.2-ml subconjunctival injection of 0.4 mg/ml or 0.1 mg/ml MMC; the contralateral eye received 0.2 ml physiological saline. Intraocula...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.197.5327
更新日期:1999-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract:AIMS:To elucidate the intramuscular distribution and branching patterns of the abducens nerve in the lateral rectus (LR) muscle so as to provide anatomical confirmation of the presence of compartmentalization, including for use in clinical applications such as botulinum toxin injections. METHODS:Thirty whole-mount hum...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1438631
更新日期:2018-06-01 00:00:00
abstract::The content of rhodopsin in the eyes of 15 donors (30 eyes) was determined. Both retinal and pigment epithelial fractions were collected from each globe, extracted using 1% CTAB, and the rhodopsin difference spectrum of each fraction was obtained separately. The total amount of rhodopsin, obtained by summing the amoun...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689009003477
更新日期:1990-12-01 00:00:00
abstract:PURPOSE:To determine the role of apoptosis in the pathogenesis of cytomegalovirus retinitis in patients with the acquired immunodeficiency syndrome. METHODS:Forty-three eyes from patients with cytomegalovirus retinitis treated before the introduction of highly active anti-retroviral therapy were examined by routine hi...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract:PURPOSE:To investigate the expression in the mouse retina and the function in a retinal cell of cullin5 that is known as a protein that is associated with ubiquitilation. METHODS:The retinal sites of expressing cullin5 were determined by immunohistochemistry with a specific antibody for cullin5. Retinal cells were tra...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.514658
更新日期:2011-01-01 00:00:00
abstract::Previous attempts to prepare monoclonal antibodies (MAbs) against S-antigen, a photoreceptor cell protein involved in the visual process and a potent autoantigen for the induction of experimental autoimmune uveitis (EAU), have yielded MAbs which define only carboxyl terminal epitopes. In this study we devised alternat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999622
更新日期:1990-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.3.231.5226
更新日期:1998-03-01 00:00:00
abstract::Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale cultures and as ascites in mice. The specificity of the antibo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000800
更新日期:1984-06-01 00:00:00
abstract:PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1250278
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To determine the role of matrix metalloproteinase 9 (MMP9) in advanced glycation end-products (AGEs) induced damage to blood-retinal barrier (BRB) and the therapeutic effects of minocycline, an MMP inhibitor, against the BRB damages. METHODS:Forty-eight Sprague Dawley rats were randomly assigned to 3 groups (1...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802450984
更新日期:2008-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1104361
更新日期:2016-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802647662
更新日期:2009-02-01 00:00:00
abstract:PURPOSE:To test the ability of two commercially available hydrogen peroxide disinfection solutions, one containing a surfactant and one without, to remove lipid from various contact lens materials using in vitro radiochemical experiments. METHODS:Etafilcon A, senofilcon A and balafilcon A contact lens materials were i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682636
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:Considerable evidence suggests that both axial and peripheral refraction play important roles in eye growth control. The large variability in peripheral refraction seen in adults and children indicates that the peripheral retina is exposed to a wide range of refractive errors. The current lack of appropriate me...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.61.15454
更新日期:2003-07-01 00:00:00
abstract:PURPOSES:To investigate whether valproic acid (VPA) has a neuroprotective effect against ischemia/reperfusion (I/R) injury in the rat retina, and to elucidate the potential antioxidant mechanisms involved. METHODS:Adult male Wistar rats were randomly divided into four groups: sham (group A), sham plus VPA (group B), I...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.653616
更新日期:2012-05-01 00:00:00
abstract:PURPOSE:Histidine is an indispensable amino acid with an imidazole ring and it is a precursor of histamine, carnosine, and anserine. Histidine has been proposed to act as a neurotransmitter or neuromodulator in mammalian central nervous system (CNS), including retina. In this study, we report histidine-like immunoreact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.6.600.5084
更新日期:1997-06-01 00:00:00
abstract::In an earlier study we isolated three cyanogen bromide cleavage fragments of bovine IRBP that exhibited high levels of immunopathogenicity, producing inflammatory changes in the eyes (EAU) and pineal gland (EAP) of Lewis rats. These fragments have been localized within the IRBP sequence. In order to identify these put...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033202
更新日期:1988-07-01 00:00:00
abstract::Purpose: To compare four visual acuity (VA) scoring termination rules. Methods: A computer simulation generated 30,000 virtual patients who underwent 10 repetitions for each of four termination rules, on both the Snellen and ETDRS charts (2.4 million tests performed in total). Three termination rules focused on the sm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1589524
更新日期:2019-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701799101
更新日期:2008-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1004720
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Anterior ischemic optic neuropathy (AION) is the most common cause of non-glaucomatous optic nerve head (ONH) injury among older adults. AION results from a sudden ischemic insult to the proximal portion of the optic nerve, typically leading to visual impairment. Here, we present an experimental model of photod...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1135960
更新日期:2016-11-01 00:00:00