Abstract:
PURPOSE/AIM:To quantitatively analyse the evolution of TUNEL-labeling, after in vivo exposure to UVB. METHODS:Altogether, 16 Sprague Dawley rats were unilaterally exposed in vivo for 15 min to close to threshold dose, 5 kJ/m(2), of ultraviolet radiation in the 300 nm wavelength region. Animals were sacrificed in groups of 4 at 1, 5, 24 and 120 h after exposure. For each animal, both eye globes were removed and frozen. The frozen eye was cryo-sectioned in 10 µm thick midsagittal sections. From each globe, three midsagittal sections with at least five sections interval in between were mounted on a microscope slide. Sections were TUNEL-labeled and counter stained with DAPI. For quantification of apoptosis, a fluorescence microscope was used. In sections with a continuous epithelial cell surface, the number of lens epithelial cell nuclei and the number of TUNEL-positive epithelial cell nuclei was counted. The total number of TUNEL-positive epithelial cell nuclei for all three sections of one lens in relation to the total number of epithelial cell nuclei for all three sections of the same lens was compared between exposed and contralateral not exposed lens for each animal. RESULTS:The relative difference of the fraction of TUNEL-positive nuclei between exposed and contralateral not exposed lens increased gradually, peaked in the time interval 5-120 h after exposure, and then declined. CONCLUSIONS:Close to threshold dose of UVB induces TUNEL-labeling that peaks in the time window 5-120 h after exposure to UVB.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Kronschläger M,Yu Z,Talebizadeh N,Meyer LM,Hallböök F,Söderberg PGdoi
10.3109/02713683.2013.783079subject
Has Abstractpub_date
2013-08-01 00:00:00pages
880-5issue
8eissn
0271-3683issn
1460-2202journal_volume
38pub_type
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journal_title:Current eye research
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doi:
更新日期:2000-01-01 00:00:00
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