Abstract:
PURPOSE:After corneal injury, persistent epithelial defects (PED's) may occur due to the chronic failure of the regenerating epithelium to adhere to the underlying stroma. The aim of this study was to examine the potential of epsilonaminocaproic acid (EACA) as a topical treatment for PED's. EACA inhibits the activation of plasmin, which metabolizes fibronectin. Fibronectin, a glycoprotein, anchors corneal epithelium to the basement membrane and the underlying stroma. METHODS:In anesthetized rabbits, PED's were induced with sodium hydroxide (1 N). Seven days later, during the late healing phase, treatment began with administration of EACA (30%) to the right eye and administration of vehicle alone to the left eye three times daily. A control group received neither EACA nor vehicle. Rabbits were treated for 19 days. PED's were visualized by fluorescein staining. Their size was mapped using digital planimetry. RESULTS:After 11 days of treatment with EACA, treated PED's were 50% smaller than in corneas treated with vehicle alone. Following treatment for 15 days, corneas treated with EACA had significantly greater re-epithelialization than vehicle-treated or control corneas. Frozen sections stained immunofluorescently for fibronectin appeared to qualitatively contain more adherent fibronectin in treated corneas. Transmission and scanning electron microscopy indicated that the epithelium was more polymorphic, thinner and vacuolated in untreated controls compared to EACA treated eyes. Light microscopy demonstrated more continuous adherent epithelium after EACA treatment. CONCLUSIONS:Topically administered EACA decreases both the severity and incidence of persistent epithelial defects produced by alkali bums to the cornea. EACA appears to promote adherence of the regenerating epithelium to the underlying stroma. Thus, topically administered EACA may be an effective treatment for this chronic condition.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Williams PB,Crouch ER Jr,Crouch ER,Mazaheri Mdoi
10.1076/ceyr.18.2.150.5382subject
Has Abstractpub_date
1999-02-01 00:00:00pages
150-7issue
2eissn
0271-3683issn
1460-2202journal_volume
18pub_type
杂志文章abstract:PURPOSE:Intravitreal injection of ciliary neurotrophic factor (CNTF) is known to induce glial intermediate filament protein (GFAP) expression in retinal Müller cells. Because CNTF binding can activate multiple signaling kinases, we have examined the involvement of JAK/STAT pathway in GFAP induction in Müller cells. ME...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.4.305.8408
更新日期:2002-04-01 00:00:00
abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408995780
更新日期:1994-03-01 00:00:00
abstract::Purpose/Aim of the study: A recently proposed technique enables the generation of continuously increasing mosaic images of the corneal sub-basal nerve plexus (SNP) using in vivo corneal confocal microscopy (CCM). The aim of the present study was to investigate the progression of the corneal nerve fiber length (CNFL) m...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1221977
更新日期:2017-04-01 00:00:00
abstract::The acyclic nucleoside phosphonate analogue PMEA [9-(2-phosphonylmethoxyethyl)adenine] is a broad spectrum antiviral agent effective against DNA viruses and retroviruses. It is particularly active against the human immunodeficiency virus and, like other phosphonylmethoxyalkyl derivatives, it also inhibits HSV-1, TK- H...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020370
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:The purpose was to investigate the effect of the superoxide dismutase mimetic tempol on decreases in retinal blood flow that are found in diabetic mice. MATERIALS AND METHODS:Streptozotocin (STZ) was injected into male C57BL/6 mice to induce hyperglycemia. One week following the STZ injection, subsets of the m...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.556300
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903509410
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:To determine whether the usual clinical dose of intravitreal dexamethasone can attenuate intraocular inflammation and retinal necrosis in a rabbit model of fulminant Bacillus cereus endophthalmitis induced by crude exotoxins. METHODS:Thirty-six eyes from pigmented mongrel rabbits received intravitreal injectio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490516701
更新日期:2004-10-01 00:00:00
abstract:PURPOSE:To investigate the expression in the mouse retina and the function in a retinal cell of cullin5 that is known as a protein that is associated with ubiquitilation. METHODS:The retinal sites of expressing cullin5 were determined by immunohistochemistry with a specific antibody for cullin5. Retinal cells were tra...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.514658
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:This study was performed to compare the positive predictive value of peripapillary retinal nerve fiber layer (RNFL) thickness measurements obtained using Cirrus high-definition optical coherence tomography (Cirrus HD-OCT; Carl Zeiss Meditec, Dublin, CA) and spectral OCT/scanning laser ophthalmoscopy (SLO) (OPKO...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713683.2013.824989
更新日期:2014-01-01 00:00:00
abstract::The morphology and postnatal ontogeny of the major pathway for aqueous outflow in the canine eye has been examined. Normal canine chamber angles of the Beagle at one day, one through ten weeks, three, four and six months, and one and two years of age were used. Angle tissue was prepared and examined by standard techni...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000791
更新日期:1984-06-01 00:00:00
abstract:PURPOSE:Diabetic maculopathy (DM) treated with photocoagulation may subsequently progress to proliferative diabetic retinopathy (PDR). However, there is insufficient knowledge about the incidence and risk factors for the development of PDR in patients previously treated for DM. MATERIALS AND METHODS:Survival data was ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1712729
更新日期:2020-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.1.26.5116
更新日期:1997-01-01 00:00:00
abstract::A method is described for the preparation of plasma membrane enriched fractions from bovine retinal pigment epithelium (RPE) by means of differential centrifugation followed by the use of self forming gradients of Percoll. A detailed analysis of the distribution of organelle specific markers (nuclei, mitochondria, lys...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000806
更新日期:1984-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.28.3.181.26247
更新日期:2004-03-01 00:00:00
abstract:PURPOSE:Klotho genes are expressed in limited tissues and have been found to be a pathogenic factor of age-related diseases in mammals, but their expressions and functions in human eyes are yet to be defined. This study was performed to investigate the expression of Klotho family genes in human ocular tissues and anter...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1259421
更新日期:2017-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.5.541.5187
更新日期:1998-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1689275
更新日期:2020-06-01 00:00:00
abstract::We adapted a previously described procedure to quantitate collagen in corneal tissue sections prepared from paraffin-embedded samples. The method entailed staining the deparaffinized tissue sections with Sirius red, eluting the bound dye with NaOH-methanol, and estimating the color in a spectrophotometer as an indicat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609015119
更新日期:1986-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609000760
更新日期:1996-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017628
更新日期:1996-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033207
更新日期:1988-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019966
更新日期:1981-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
abstract:PURPOSE:The fibroblast growth factors (FGFs) are a family of 9 heparin binding proteins which have been proposed to play key roles in angiogenesis. Basic FGF (bFGF), acidic FGF (aFGF) and FGF5 have previously been demonstrated to be expressed in the normal retina and RPE. In this study, the expression of FGF5 was explo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.4.396.10685
更新日期:1997-04-01 00:00:00
abstract::The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003066
更新日期:1984-04-01 00:00:00
abstract:PURPOSE:Hypercarbia has been suggested as a risk factor for retinopathy of prematurity (ROP). We investigated the effect of hypercarbia on the retinal vasculature of the neonatal rat to determine whether hypercarbia alone could induce preretinal neovascularization analogous to ROP. METHODS:In a preliminary blood gas s...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The intraocular inflammatory reaction in a murine model of herpes simplex keratouveitis was studied using immunohistochemical techniques. 5 x 10(5) PFU of HSV-1 (Stewart strain) was applied to the abraded cornea of BALB/c mice. The subsequent development of keratouveitis was documented by sequential examination, conve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309024629
更新日期:1993-05-01 00:00:00
abstract::The present study was aimed to demonstrate that cytochrome P-450 was induced by drug administration in particular tissues in the eye. Phenobarbital was used as an inducer. We showed immunohistochemically that cytochrome P-450 was induced in the cornea, conjunctiva and ciliary epithelium of rabbits after four days of i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034854
更新日期:1987-07-01 00:00:00
abstract::The goal of this study was to determine the effect of extracellular matrix components on the phagocytic function of the retinal pigment epithelial (RPE) cell. Cultured human fetal RPE cells were established in culture and plated on three commercially-prepared substrates: collagen IV, fibronectin and laminin and on thr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017607
更新日期:1996-01-01 00:00:00