Abstract:
:Penetrating keratoplasty was the only therapeutic choice for the treatment of corneal endothelial decompensation until the introduction of evolutional endothelial keratoplasties, namely Descemet's stripping automated endothelial keratoplasty (DSAEK) and Descemet's membrane endothelial keratoplasty (DMEK). Although now in widespread use, DSAEK and DMEK still have associated problems, such as difficulty of the surgical technique, acute and chronic cell loss, and shortage of donor corneas. Therefore, regeneration of the corneal endothelium by tissue engineering techniques is being researched to overcome these problems. The concept of transplantation of cultured corneal endothelial cells (CECs) was proposed in the 1970s. However, cultivation of human CECs (HCECs) in sufficient quantity and with acceptable quality for clinical use has proven surprisingly difficult, and the development of methods for transplanting cultured HCECs has been necessary. Numerous research groups have developed culture protocols and techniques that are now bringing corneal endothelial regeneration closer to real-world therapy. For instance, we started a clinical trial in 2013 involving the injection of cultured HCECs into the anterior chamber of patients with corneal endothelial decompensation. This review outlines the rapid progression of this research field, including clinical trial results, and is also intended to identify topics that still require further research or discussion.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Okumura N,Koizumi Ndoi
10.1080/02713683.2019.1700529subject
Has Abstractpub_date
2020-03-01 00:00:00pages
303-312issue
3eissn
0271-3683issn
1460-2202journal_volume
45pub_type
杂志文章abstract:PURPOSE:To generate toxicology profiles of individual drugs on human corneal epithelial cells (HCEC) and compare their in vitro cytotoxicity. METHODS:Monolayer cultures of HCEC were harvested from two human donor eyes. Sunitinib (0.3-10 µg/mL) and Sorafenib (0.3-100 µg/mL), diluted in culture medium (CnT-BM.1, CELLnTE...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.833629
更新日期:2014-02-01 00:00:00
abstract:PURPOSE:Considerable evidence suggests that both axial and peripheral refraction play important roles in eye growth control. The large variability in peripheral refraction seen in adults and children indicates that the peripheral retina is exposed to a wide range of refractive errors. The current lack of appropriate me...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.61.15454
更新日期:2003-07-01 00:00:00
abstract::Purpose/Aim: To assess the changes in anterior eye segment biometrics and intraocular pressure during and after wearing swimming goggles.Materials and methods: Anterior segment biometry and intraocular pressure were assessed in 20 healthy young adults (22.2 ± 4.7 years) during the wearing of a drilled swimming goggle....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1662056
更新日期:2020-05-01 00:00:00
abstract::Purpose: To summarize the research status on the function and mechanism of circRNAs in regulating the occurrence of diabetic retinopathy (DR). Methods: We systematically searched PubMed, Embase.com, and ARVO Abstracts website and reviewed relevant studies. Results: Thousands of circRNAs were found to be aberrantly exp...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1812086
更新日期:2020-08-30 00:00:00
abstract:PURPOSE:Accumulation of lipoprotein-derived lipids including esterified and unesterified cholesterol in Bruch's membrane of human eyes is a major age-related change involved in initiating and sustaining soft drusen in age-related macular degeneration (AMD). The apolipoprotein (apo) A-I mimetic peptide 4F is a small ant...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1370118
更新日期:2018-01-01 00:00:00
abstract::Purpose/Aim: Corneal abrasions and nonhealing corneal epithelial defects are common conditions that cause pain and sometimes are slow to heal. Histatins, a family of histidine-rich peptides, have been implicated in oral and skin epithelial wound healing, and have been shown to be effective in vitro in human corneal ep...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1490772
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:Histidine is an indispensable amino acid with an imidazole ring and it is a precursor of histamine, carnosine, and anserine. Histidine has been proposed to act as a neurotransmitter or neuromodulator in mammalian central nervous system (CNS), including retina. In this study, we report histidine-like immunoreact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.6.600.5084
更新日期:1997-06-01 00:00:00
abstract:PURPOSE:To investigate the effects of citicoline on upregulated clusterin and retinal damage induced by kainic acid (KA). METHODS:KA was injected into the vitreous of rats. Effects of systemic citicoline treatments were estimated by measuring the thickness of the various retinal layers, immunoblotting, and immunohisto...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701758719
更新日期:2007-12-01 00:00:00
abstract:PURPOSE:To investigate the characteristics of anterior chamber inflammatory reaction induced by intravitreal injection of endothelin-1 (ET-1). METHODS:The time course of changes in aqueous protein concentration (APC) after intravitreal injection of 10(-4), 10(-5), 10(-6) and 10(-7) M ET-1 into rabbit eyes was measured...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.2.205.5612
更新日期:1998-02-01 00:00:00
abstract::The full length cDNA sequence for the lens-specific intermediate filament protein, CP49, from chicken is presented. The sequence contains features typical of the other intermediate filament proteins, including two major alpha-helical regions, helix I and II and appropriate linker regions. CP49 lacks a C-terminal non-a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508998401
更新日期:1995-07-01 00:00:00
abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514055
更新日期:2018-12-01 00:00:00
abstract::Recently, growth factors are known to phosphorylate tyrosine residues of proteins to regulate cellular functions. We investigated growth factor-dependent phosphorylation of membrane proteins in cultured human retinal pigment epithelial (RPE) cells. The phosphorylation experiments were done in membrane preparations of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209033498
更新日期:1992-10-01 00:00:00
abstract:PURPOSE:To evaluate postnatal developmental changes in the amounts of retinal glutamate, glutamine and GABA, and in the distribution of retinal glutamine in the rat. METHODS:Free amino acids were extracted from rat retinas of different postnatal stages, and the concentrations of glutamate, glutamine and GABA were dete...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609008907
更新日期:1996-06-01 00:00:00
abstract::Na/K ATPase activity has been determined in normal and cataractous human lenses using labeled ATP as a substrate. The enzyme is distributed throughout the lens with approximately 1/3 of the total activity in the capsule-epithelium and 1/2 of the activity in the cortex. Furthermore, the activity of Na/K ATPase decrease...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209000778
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:To quantify levels of matricellular proteins in aqueous humor samples from acute primary angle closure (APAC) and non-glaucomatous cataract eyes and investigate their correlation with intraocular pressure (IOP) fluctuation. MATERIALS AND METHODS:Aqueous humor samples were collected from 63 eyes including 29 cu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1449222
更新日期:2018-06-01 00:00:00
abstract::Systemically administered fluorescein is converted to fluorescein glucuronide. The conjugate is fluorescent and interferes with the measurement of fluorescein in the anterior chamber. Carboxyfluorescein is a hydrophilic derivative of fluorescein. In the rabbit, carboxyfluorescein is not converted as readily to a fluor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609025171
更新日期:1986-05-01 00:00:00
abstract:PURPOSE:Corneal wound healing after excimer laser photorefractive keratectomy (PRK) passes through a series of characteristic stages which have earlier been defined by means of histological, histochemical, and biochemical approaches. We investigated the potential of confocal microscopy to verify morphological changes i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.7.640.5058
更新日期:1997-07-01 00:00:00
abstract:PURPOSE:To establish autologous iris pigment epithelial (IPE) cell transplantation in monkey eyes. METHODS:Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cell culture was performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To characterize the effect of glutamate receptor activation/inhibition on the secretion of vascular endothelial growth factor (VEGF) in retina-specific glial (Müller) cells under experimental conditions of hyperglycemia and hypoxia, two intrinsic pathologic conditions of diabetic retinopathy. METHODS:Purified ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.483312
更新日期:2010-08-01 00:00:00
abstract:PURPOSE:Dry eye is a common condition known to have many systemic co-morbidities. We aim to report the frequency of depression and anxiety suspects in eye clinic patients and explore the association between dry eye signs and symptoms with depression and anxiety. MATERIALS AND METHODS:Adult outpatients were recruited f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1056804
更新日期:2016-05-01 00:00:00
abstract::Purpose: To determine the refractive astigmatism following toric intraocular lens (tIOL) implantation without consideration of posterior keratometric astigmatism with a conventional tIOL calculator for eyes with low keratometric astigmatism (0.75D to 2.5D) and to theoretically compare the outcomes with predicted refra...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1638418
更新日期:2019-12-01 00:00:00
abstract::Purpose: To evaluate the long-term efficacy and safety of the modified canaloplasty technique with additional suprachoroidal drainage in patients with pseudoexfoliation glaucoma. Methods: This retrospective clinical trial included 131 patients with pseudoexfoliation glaucoma who underwent canaloplasty with suprachoroi...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1795203
更新日期:2021-02-01 00:00:00
abstract::We established growth curves for Propionibacterium acnes isolates recovered from eyes with chronic postoperative endophthalmitis. The growth curve plotted the average of the duplicate bacterial concentration against time. The generation time for P. acnes calculated from the growth curves was approximately 5.1 hours. T...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999875
更新日期:1994-06-01 00:00:00
abstract:PURPOSE:To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis. MATER...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802647662
更新日期:2009-02-01 00:00:00
abstract:PURPOSE:In the limbo-corneal epithelium the stem and early precursor epithelial cell pool is confined to the limbal rim. Among the features associated with this spatial segregation is the general paucity of connexin43 (Cx43) within the limbal basal cell population and its complete absence in resident stem cells. The li...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.6.430.8599
更新日期:2002-06-01 00:00:00
abstract::Adult albino mice received tritiated thymidine either injected subcutaneously (333 microCi) every 6 hours for up to 36 hours, or administered in their drinking water for up to 74 days (about 45 microCi/day). Tissue sections were examined with autoradiography to determine the distribution of the label. Animals receivin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809047029
更新日期:1988-03-01 00:00:00
abstract:PURPOSE:The purposes of this study were to investigate the effects of topically administrated Tacrolimus and Octreotide on modulation of postoperative scarring in experimental glaucoma filtration surgery and to compare the antifibrotic properties of these agents with mitomycin-C (MMC). MATERIAL AND METHODS:A total of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.635404
更新日期:2012-03-01 00:00:00
abstract::Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale cultures and as ascites in mice. The specificity of the antibo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000800
更新日期:1984-06-01 00:00:00
abstract::From knowledge of the optical parameters of experimental animal eyes and radiometric parameters of the light source, it is possible to provide accurate retinal irradiances (dose rates). Examples are given for the rat, cat, monkey and other animals of interest. Fluorescent lamps and incandescent lamp radiances are prov...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688408997199
更新日期:1984-01-01 00:00:00
abstract:PURPOSE:To assess the effect of aging on optical quality in a normal population. METHODS:We prospectively examined 100 normal eyes of 100 healthy volunteers (50 men, 50 women; ages, 20-69 years; mean age ± standard deviation, 44.6 ± 15.5 years). We quantitatively assessed the values of MTF cutoff frequency, Strehl rat...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713683.2012.688164
更新日期:2012-10-01 00:00:00