Serum-free culture of porcine and rabbit corneal epithelial cells.

Abstract:

:To better define the growth requirements for corneal epithelial cells, methods for serum-free culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin/EDTA. Growth of cells with epithelial-like morphology was readily achieved in low calcium MCDB 153 medium containing epidermal growth factor, insulin, hydrocortisone, and bovine pituitary extract. Primary cultures could be subcultivated at least four times at 1:6 split ratios. Examination of cultured corneal epithelial cells by transmission electron microscopy demonstrated a relatively undifferentiated phenotype. Elevation of calcium in the medium caused the reappearance of desmosomal junctions and a more typical corneal epithelial morphology.

journal_name

Curr Eye Res

journal_title

Current eye research

authors

Hackworth LA,Faraji-Shadan F,Schuschereba ST,Bowman PD

doi

10.3109/02713689008999564

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

919-23

issue

9

eissn

0271-3683

issn

1460-2202

journal_volume

9

pub_type

杂志文章
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