Abstract:
:To investigate the role of lymphocytes in the pathogenesis of uveitis, we analyzed the expression of memory markers, CD29 and CD45RO antigens, and apoptosis-related Fas antigen on T lymphocytes in the aqueous humor (AH) and peripheral blood (PB) from patients with uveitis. Using three-color flow cytometry, we assessed the number of T lymphocyte subsets that stained with fluorescence-conjugated anti-CD3, CD4, CD8, CD29, CD45RA, CD45RO, HLA-DR, and Fas monoclonal antibodies in the AH and PB from 19 patients with active uveitis who were diagnosed as having sarcoidosis, Vogt-Koyanagi-Harada disease, HLA-B27+ uveitis, or idiopathic uveitis. Cells from AH and PB were evaluated by light and electron microscopy before and after 6 h of incubation. The majority of lymphocytes in AH but not in PB, were CD3+HLA-DR+ (activated) T cells. The percentage of CD4+ lymphocytes was significantly higher in uveitic AH than in PBL (P < 0.01). While the percentage of CD4+ CD45RA+ (naive) cells within T cells was much lower in uveitic AH than in PB, the percentage of CD4+CD29+ or CD4+CD45RO+ (memory) cells was significantly higher in uveitic AH than in PBL (P < 0.01). Fas antigen was expressed preferentially on memory cells in uveitic AH. Apoptosis of cells in the AH was observed by microscopically following after incubation with no stimulation. Lymphocytes from the AH of patients with uveitis were more activated than those from PB. The majority of T lymphocytes from uveitic AH expressed memory markers and Fas antigen. Results suggest that an increase in the number of Fas+ memory T lymphocytes in AH is involved in the pathogenesis of uveitis.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Ohta K,Norose K,Wang XC,Ito S,Yano A,Segawa Kdoi
10.3109/02713689609007624subject
Has Abstractpub_date
1996-03-01 00:00:00pages
299-306issue
3eissn
0271-3683issn
1460-2202journal_volume
15pub_type
杂志文章abstract::The short and long term response of the trabecular meshwork to a phagocytic challenge and the response of the meshwork to different types of foreign particles was studied by injecting one eye of 25 adult cats with a phagocytic agent (zymosan, blood, or latex microspheres) while the fellow eye received a control soluti...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909000037
更新日期:1989-11-01 00:00:00
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journal_title:Current eye research
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doi:10.1080/02713683.2020.1820526
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209001812
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journal_title:Current eye research
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doi:10.1080/02713683.2020.1849728
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.3109/02713683.2013.841255
更新日期:2014-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020325
更新日期:1991-10-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999411
更新日期:1990-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,meta分析
doi:10.1080/02713683.2019.1570274
更新日期:2019-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.7.640.5058
更新日期:1997-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.548892
更新日期:2011-04-01 00:00:00
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journal_title:Current eye research
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doi:10.1080/02713680600760493
更新日期:2006-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.1.12.5426
更新日期:2002-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.5.428.7048
更新日期:1997-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608995831
更新日期:1996-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1773866
更新日期:2020-10-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713688609015101
更新日期:1986-02-01 00:00:00
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journal_title:Current eye research
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journal_title:Current eye research
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journal_title:Current eye research
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更新日期:1984-01-01 00:00:00
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journal_title:Current eye research
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更新日期:1997-01-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713689609017628
更新日期:1996-08-01 00:00:00
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journal_title:Current eye research
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Current eye research
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更新日期:2018-12-01 00:00:00