Abstract:
PURPOSE:To determine the roles of the murine interleukin-8 receptor homolog (mIL-8Rh, neutrophil chemokine CXC receptor 2) and macrophage inflammatory protein-1alpha (MIP-1alpha, a CC chemokine) in two eye inflammation models: endotoxin-induced uveitis (EIU) and immune complex-induced uveitis (reverse passive Arthus reaction (RPAR) uveitis). METHODS:For the EIU model, 250 ng E.coli endotoxin was injected into the vitreous of mIL-8Rh-/- mice or heterozygous littermate mIL-8Rh+/- controls and into MIP-1alpha-/- mice or congenic MIP-1alpha+/+ controls. Eyes were harvested after 24 h for histologic characterization of infiltrating cells and IL-6 bioassays. For the RPAR model, mouse antiserum against human serum albumin (HSA) was injected into the vitreous of mIL-8Rh-/-, mIL-8Rh+/-, MIP-1alpha-/-, and MIP-1alpha+/+ mice. Twenty-four hours later, animals were challenged with intravenous HSA. Eyes were harvested after 4 h for analysis. RESULTS:RPAR resulted in the deposition of immune complexes at the ciliary area and iris with the subsequent development of uveitis. Genetic deficiency of mIL-8Rh reduced the median number of infiltrating cells in EIU by 63% (p < 0.01) but had no effect on RPAR-induced inflammation. In the EIU model, macrophages comprised a much higher percentage (45%) of infiltrating cells in mice lacking mIL-8Rh than in controls (17%). Loss of the MIP-1alpha gene had no apparent effect on RPAR uveitis and a 39% reduction of infiltrating cells in EIU that was not statistically significant. IL-6 activity in aqueous humor was much less in mice with RPAR uveitis than in those with EIU. Neither gene deletion had a significant impact on IL-6 levels in either disease model. CONCLUSIONS:Chemokines acting via mIL-8Rh have a significant role in the induction of neutrophil infiltration during EIU but not during RPAR uveitis. MIP-1alpha is not critical for either EIU or RPAR-induced uveitis. The differential dependence on IL-8-like chemokines is in accord with the two forms ofuveitis having different etiologies and, therefore, potentially different optimal therapies.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Brito BE,O'Rourke LM,Pan Y,Huang X,Park JM,Zamora D,Cook DN,Planck SR,Rosenbaum JTdoi
10.1076/ceyr.19.1.76.5339subject
Has Abstractpub_date
1999-07-01 00:00:00pages
76-85issue
1eissn
0271-3683issn
1460-2202journal_volume
19pub_type
杂志文章abstract:PURPOSE:This study was designed to investigate whether synthetic small interference RNA (siRNA) targeting VEGF-A could inhibit mouse corneal neovascularization or not. METHODS:First, the effect of synthetic siRNA targeting VEGF-A on the expression of VEGF in human retinal pigment epithelial line ARPE-19 cells was exam...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003597230
更新日期:2010-05-01 00:00:00
abstract::Changes in the content of calcitonin gene-related polypeptide (CGRP) and tyrosine hydroxylase (TH) immunoreactivities in the anterior segment of the rat eye were assessed histochemically in animals subjected to sympathetic ganglionectomy or to chronic sensory denervation induced by neonatal capsaicin treatment. In the...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033207
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate how glutathione reductase (GR) loses its activity during cataract formation and whether it is possible to revive it back to the normal levels. METHOD:In this study, endogenous as well as synthetic reducing systems (GSH, TTase, DTT, captopril) and alpha-crystallin at dif...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590953110
更新日期:2005-10-01 00:00:00
abstract:PURPOSE:The expression of human leukocyte antigen (HLA) class II molecules on the cell surface is necessary for the presentation of peptide antigens to helper CD4 T lymphocytes of the immune system. We studied the immunoexpression of HLA class II antigen in conjunctival precursor lesions and conjunctival squamous cell ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.3.151.16052
更新日期:2003-09-01 00:00:00
abstract:PURPOSE:To evaluate the effect of anterior migration of triamcinolone acetonide on intraocular pressure (IOP) elevation after posterior subtenon injection of triamcinolone acetonide (PSTA) for macular edema. METHODS:One hundred and ten eyes from 89 patients who received PSTA for macular edema were prospectively enroll...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1826979
更新日期:2020-09-27 00:00:00
abstract::Intraocular synthesis of IgG antibodies against HSV (herpes simplex virus), CMV (cytomegalovirus) and VZV (varicella zoster virus) is considered as an indirect proof of uveoretinal infection. Paired serum and aqueous samples obtained from 16 patients with retinitis associated with AIDS, 3 patients with ARN, 8 patients...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999412
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:This study was designed to investigate the presence of the secretory phospholipase A2 receptor in RPE, a protein closely related to the phagocytic mannose receptor. METHODS:Proteins from cultured rat, pig, and human RPE were separated by SDS-PAGE and immunoblotted with a polyclonal guinea pig sPLA2 receptor an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.1.1.5345
更新日期:1999-07-01 00:00:00
abstract::Uveal melanoma develops in one of the most capillary-rich tissues of the body and is disseminated hematogenously. Knowledge of the nature and the spatiotemporal expression of angiogenic factors in uveal melanoma is essential to the development of new treatment strategies, especially with regard to improving survival. ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600865052
更新日期:2006-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1185129
更新日期:2017-03-01 00:00:00
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journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713688809033194
更新日期:1988-07-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the effect of vitreous reflux (VR) on the short-term effect of intravitreal ranibizumab injection. MATERIALS AND METHODS:The study included 181 eyes of 81 age-related macular degeneration (AMD) and 100 diabetic macular edema (DME) patients. Treatment response was evaluated...
journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2011-12-01 00:00:00
abstract::Transforming growth factor-beta (TGF-beta), a multifunctional cytokine which has been identified in normal and inflamed ocular fluids, may play a role in the evolution of inflammatory ocular lesions. In this study we utilized a rabbit model of LPS-induced uveitis to determine if exogenous TGF-beta 2 could alter its co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017616
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To assess changes in retinal vascular caliber in response to short-term use of nepafenac eye drops in patients with mild diabetic macular edema. MATERIALS AND METHODS:Thirty-four patients with previously untreated bilateral mild diabetic macular edema were included in this prospective study. For each participa...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2017.1399425
更新日期:2018-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.700755
更新日期:2012-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033223
更新日期:1988-12-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608995151
更新日期:1996-12-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.1.23.9966
更新日期:2002-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490503769
更新日期:2004-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.5.503.7049
更新日期:1997-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689009000051
更新日期:1990-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688808995738
更新日期:1988-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.10.992.9021
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:To investigate the potential toxicity of the vehicle used for triamcinolone acetonide (TA) to the cornea, lens, ciliary body, and retina of pigmented rabbits. METHODS:Forty chinchilla rabbits (40 eyes) were divided into four groups: group A (control group) eyes received an intravitreal injection of 0.1 ml ster...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680903063082
更新日期:2009-09-01 00:00:00
abstract::Na/K ATPase activity has been determined in normal and cataractous human lenses using labeled ATP as a substrate. The enzyme is distributed throughout the lens with approximately 1/3 of the total activity in the capsule-epithelium and 1/2 of the activity in the cortex. Furthermore, the activity of Na/K ATPase decrease...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209000778
更新日期:1982-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.3109/02713683.2013.841255
更新日期:2014-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.2.102.5086
更新日期:1997-02-01 00:00:00
abstract::Penetrating keratoplasty was the only therapeutic choice for the treatment of corneal endothelial decompensation until the introduction of evolutional endothelial keratoplasties, namely Descemet's stripping automated endothelial keratoplasty (DSAEK) and Descemet's membrane endothelial keratoplasty (DMEK). Although now...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1700529
更新日期:2020-03-01 00:00:00
abstract::Glaucoma is increasingly recognized as a neurodegenerative disorder, characterized by the accelerated loss of retinal ganglion cells (RGCs) and their axons. Impaired axonal transport has been implicated as a pathogenic mechanism in a number of neurodegenerative diseases, including glaucoma. The long RGC axon, with its...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713683.2015.1037924
更新日期:2016-01-01 00:00:00