Abstract:
PURPOSE:Pigmentation of the iris is caused by varying amounts of melanin pigment granula in a constant number of melanocytes in the superficial stroma. Melanin has been shown to act as antioxidant. We have now investigated lipid peroxidation in dependence on stromal pigmentation in isolated porcine irises. METHODS:The same number of lightly and heavily pigmented porcine irises (visual selection) were homogenized (1 : 20 w/v) in buffer (50 mmol/l phosphate buffer and 4 mmol/l sodium azide). 500 microl homogenate were incubated at 37 degrees C in duplicate for 5, 10, 20 and 40 min in absence and presence of Fe2+ as inducer of lipid peroxidation. The amount of lipid peroxidation was assayed by the thiobarbituric acid (TBA) test. The results are expressed as nmol of TBA reactive material (TBAR) produced/mg protein. Fe2+ concentration of the supernatant was determined spectrophotometrically with 1,10 orthophenanthroline. Concentrations of D-glucose and D -fructose in iris tissue homogenates were determined spectrophotometrically by enzymatic bioanalysis. RESULTS:70, 180 and 360 micromol/l Fe2+ induced lipid peroxidation. A plateau region was reached after 20 min. The amount of lipid peroxidation differed in dependence on stromal pigmentation in porcine irises. The effect was most significant at 180 micromol/l Fe2+, which induced 1.373 +/- 0.138 nmol TBAR/mg protein in lightly compared to 0.491 +/- 0.125 nmol TBAR/mg protein in heavily pigmented irises after 10 min incubation (p < 0.0001, n = 4). Similar effects (factor 2-3) were also measured after 20 and 40 min incubation. On the other hand, the content of Fe2+ in the supernatant was the same within error. Sugar concentrations (D-glucose and D-fructose) did not differ significantly for the two differently pigmented iris tissues. CONCLUSIONS:There is a stronger induction of lipid peroxidation in lightly compared to heavily pigmented porcine irises. This effect may be related to the difference in stromal melanin content and its antioxidant activity.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Nau-Staudt K,Nau WM,Haefliger IO,Flammer Jdoi
10.1076/ceyr.22.3.229.5511subject
Has Abstractpub_date
2001-03-01 00:00:00pages
229-34issue
3eissn
0271-3683issn
1460-2202journal_volume
22pub_type
杂志文章abstract:PURPOSE:The high prevalence of positive family history of keratoconus (KC) in KC patients is well-known. However, the results regarding the association between family history of KC and disease severity are controversial. The aim of this study was to evaluate the possible association between family history and severity ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1128553
更新日期:2016-11-01 00:00:00
abstract::DNA sequence analysis of overlapping shotgun and restriction fragments have revealed the entire sequence of the human lens fiber cell intrinsic membrane protein MP19 gene (also termed MP17, MP18, and MP20). The 8,056 bp MP19 gene contains 5 exons encoding a mature protein of 173 amino acids, which displayed a very hig...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309033503
更新日期:1993-12-01 00:00:00
abstract::Glaucoma is increasingly recognized as a neurodegenerative disorder, characterized by the accelerated loss of retinal ganglion cells (RGCs) and their axons. Impaired axonal transport has been implicated as a pathogenic mechanism in a number of neurodegenerative diseases, including glaucoma. The long RGC axon, with its...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713683.2015.1037924
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Interleukin-6 (IL-6) is produced by a variety of cells involved in inflammation and acts as local intensification signals in pathological processes associated with chronic eye inflammation. This meta-analysis was performed to provide a better understanding of the relationship between IL-6 and diabetic retino...
journal_title:Current eye research
pub_type: 杂志文章,meta分析
doi:10.1080/02713683.2019.1570274
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:Considerable evidence suggests that both axial and peripheral refraction play important roles in eye growth control. The large variability in peripheral refraction seen in adults and children indicates that the peripheral retina is exposed to a wide range of refractive errors. The current lack of appropriate me...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.61.15454
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:The aim of the present study was to evaluate the efficacy of the inverted internal limiting membrane (ILM) flap technique that involved the use of a perfluorocarbon liquid (PFCL) bubble for the selective prevention of indocyanine green (ICG) staining of the parts of the inverted flap in contact with the retinal...
journal_title:Current eye research
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doi:10.1080/02713683.2018.1524014
更新日期:2019-01-01 00:00:00
abstract::The cell content of the precorneal tears of eight subjects was sampled following overnight wear of a contact lens in one eye. This was compared with morning samples taken from the other eye, and afternoon samples taken several days later from both eyes. In the morning the median number of desquamated epithelial cells ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908996380
更新日期:1989-04-01 00:00:00
abstract:PURPOSE:To study the association of cell proliferation in uveal melanoma with the mean of the 10 largest nucleoli (MLN), microvascular prognostic factors, and survival. METHODS:Population-based, retrospective cohort study of 167 choroidal and ciliary body melanomas enucleated from 1972 to 1981. Mouse monoclonal antibo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680500478535
更新日期:2006-01-01 00:00:00
abstract::Recently, growth factors are known to phosphorylate tyrosine residues of proteins to regulate cellular functions. We investigated growth factor-dependent phosphorylation of membrane proteins in cultured human retinal pigment epithelial (RPE) cells. The phosphorylation experiments were done in membrane preparations of ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209033498
更新日期:1992-10-01 00:00:00
abstract:PURPOSE:To test the ability of two commercially available hydrogen peroxide disinfection solutions, one containing a surfactant and one without, to remove lipid from various contact lens materials using in vitro radiochemical experiments. METHODS:Etafilcon A, senofilcon A and balafilcon A contact lens materials were i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682636
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:Intrastromal epithelial migration occurs following deep excimer annular keratectomy. Our purpose is to determine the localization of gelatinase B expression and its relationship to migrating intrastromal epithelial cells following deep annular excimer keratectomy. METHODS:Rabbit corneas were treated with deep ...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::The intraocular inflammatory reaction in a murine model of herpes simplex keratouveitis was studied using immunohistochemical techniques. 5 x 10(5) PFU of HSV-1 (Stewart strain) was applied to the abraded cornea of BALB/c mice. The subsequent development of keratouveitis was documented by sequential examination, conve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:Previous studies have suggested that corneal fibrosis controlled by a TGFbeta-mediated cytocrine pathway underlies the development of clinical corneal haze and associated regression of photoablative effect following excimer laser PRK. Using a unique blocking antibody, we evaluated the role of TGFbeta in post-PR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:PURPOSE:To evaluate how corneal deturgescence after Descemet membrane endothelial keratoplasty (DMEK) influences the posterior corneal surface area and the endothelial cell density (ECD) measurements. MATERIALS AND METHODS:A mathematical model was formulated to estimate the increase in posterior corneal surface area a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.753095
更新日期:2013-02-01 00:00:00
abstract:PURPOSE:To investigate the biomechanical effect induced by radial keratotomy on outflow facility in paired, enucleated whole porcine eyes. METHODS:Freshly enucleated porcine eyes were perfused at a constant pressure of 10 mm Hg. Radial keratotomy (RK), with a 3.5 mm central clear zone and eight radial incisions, was p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.12.1193.5027
更新日期:1997-12-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405045
更新日期:2018-03-01 00:00:00
abstract::Purified Pseudomonas aeruginosa elastase cleaved a 65 kDa gelatinase [inactive proenzyme form of matrix metalloproteinase (MMP-2)] from human corneal fibroblasts into a biologically active fragment with an approximate molecular mass of 58 kDa. However, purified pseudomonal alkaline protease did not cleave MMP-2 apprec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209015082
更新日期:1992-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.907430
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:So far tear substitutes have demonstrated a limited role in restoring ocular surface damage in dry eye syndrome (DES). The aim of this study was to assess the efficacy of a new ocular surface modulator in an in vitro model of human corneal epithelium (HCE) damaged by severe osmotic stress mirroring the features...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1184282
更新日期:2017-03-01 00:00:00
abstract::Processing of visual information within the retina depends in large measure upon a complement of chemical neurotransmitters which are released at synaptic contacts between individual neurons. Numerous investigators have participated in the identification of many of these transmitters and their assignment to specific n...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713689609003465
更新日期:1996-07-01 00:00:00
abstract::Using phosphorus-31 nuclear magnetic resonance (P-31 NMR) spectroscopy the rat crystalline lens was evaluated as a potential animal model for the study of lens metabolic processes relevant to the human. The P-31 NMR spectra of intact rat lenses and rat lens PCA extracts provided comparable qualitative and quantitative...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019974
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:We wished to establish which matrix metalloproteinases (MMPs) and metalloproteinase inhibitors (TIMPs) were present in human interphotoreceptor matrix (IPM) and vitreous. METHODS:IPM and vitreous were obtained from postmortem human eyebank eyes. Western immunoblots were probed with antibodies against human MMP...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.2.132.5610
更新日期:1998-02-01 00:00:00
abstract::The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003066
更新日期:1984-04-01 00:00:00
abstract::Two alternative methods for the study of the lens cytoskeleton are described which serve to overcome some of the difficulties imparted by the unique biology of the lens. The first technique involves rapid freezing, thick sectioning, and selective extraction and/or fixation of the lens section. This approach offers sev...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997582
更新日期:1990-11-01 00:00:00
abstract::The pigmented epithelium of the vertebrate retina phagocytizes the discarded tips of photoreceptors and it is likely that a specific cellular recognition process is involved in this phenomenon. The apical surface of retinal pigmented epithelium (RPE) contains microvilli which interdigitate with the outer segment regio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034868
更新日期:1987-08-01 00:00:00
abstract::Antifibrosis agents have improved the success of glaucoma filtration surgery, although undesired side effects are not readily reversible and may present a major limitation in the use of these agents. Our purpose was to study the efficacy of cytosine arabinoside (Ara-C) as an adjunctive antimetabolite in glaucoma surge...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409025140
更新日期:1994-11-01 00:00:00
abstract::The immunochemical reactivities of the alpha A and alpha B subunits from bovine alpha-crystallin have been compared using 5 monoclonal antibodies and 2 polyvalent antisera. Each subunit bound the same maximum amount of antibody, regardless of its source, and each subunit was able to completely displace alpha-crystalli...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609020047
更新日期:1986-03-01 00:00:00
abstract::The effects of 4% epinephrine bitartrate and 0.5% timolol on ocular and optic nerve blood flow were studied in phakic and aphakic rabbit eyes using a radioactive microsphere (85Sr) technique. Either epinephrine or timolol was given topically as eight drops in the hour immediately preceding blood flow measurements (one...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000822
更新日期:1984-10-01 00:00:00
abstract:PURPOSE:Most studies of cell growth of the RPE employ cultures which have been previously passaged, but here we investigated freshly-explanted RPE cells, which may have been growth-quiescent for years, within the eye to determine whether co-culture affects initial outgrowth in primary culture. METHODS:Bovine or human ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.9.891.5051
更新日期:1997-09-01 00:00:00
abstract:PURPOSE:To investigate the efficacy of intravitreal piperacillin/tazobactam (250 microg/0.1 ml) in the treatment of experimental Pseudomonas aeruginosa endophthalmitis in rabbits. MATERIALS AND METHODS:Twenty New Zealand White albino rabbits were used in this study. The rabbits were divided into two groups (10 rabbits...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490894180
更新日期:2005-01-01 00:00:00