Abstract:
:The pigmented epithelium of the vertebrate retina phagocytizes the discarded tips of photoreceptors and it is likely that a specific cellular recognition process is involved in this phenomenon. The apical surface of retinal pigmented epithelium (RPE) contains microvilli which interdigitate with the outer segment regions of photoreceptor cells and it is this apical microvillous surface that is of particular interest with respect to phagocytosis. The present study is a report of a method to isolate a fraction that is enriched in microvilli from the apical surface of this highly polarized epithelial cell. Wheat germ agglutinin (WGA) conjugated sepharose beads are used to remove the microvillous membranes which are then observed with scanning and transmission electron microscopy. The proteins of this RPE-subfraction are separated through use of SDS-polyacrylamide gel electrophoresis. The relative molecular weights (Mr) and lectin binding properties of glycoproteins are examined in Western blots through the use of lectin-peroxidase conjugates as probes for carbohydrate residues. A preliminary comparison of membranes isolated from Long Evans (normal) and Royal College of Surgeons (dystrophic) rat retina RPE shows that the glycoproteins in these two preparations are different with respect to the binding of Concanavalin-A (Con-A) and WGA. In particular a glycoprotein in the normal RPE preparation with a Mr of 175K binds Con-A and WGA, but in the dystrophic RPE preparation binds little or no WGA. A glycoprotein present in the normal RPE preparation with a Mr of 86K binds Con-A and WGA, but both lectins have reduced binding sites in the dystrophic preparation. Limax flavus agglutinin (specific for sialic acid residues) binds to a high molecular weight glycoprotein with a Mr of 195K-196K which is present in both normal and dystrophic RPE membrane preparations and which also binds Con-A and WGA.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Cooper NG,Tarnowski BI,McLaughlin BJdoi
10.3109/02713688709034868subject
Has Abstractpub_date
1987-08-01 00:00:00pages
969-79issue
8eissn
0271-3683issn
1460-2202journal_volume
6pub_type
杂志文章abstract::Intraocular synthesis of IgG antibodies against HSV (herpes simplex virus), CMV (cytomegalovirus) and VZV (varicella zoster virus) is considered as an indirect proof of uveoretinal infection. Paired serum and aqueous samples obtained from 16 patients with retinitis associated with AIDS, 3 patients with ARN, 8 patients...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999412
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:To test the ability of two commercially available hydrogen peroxide disinfection solutions, one containing a surfactant and one without, to remove lipid from various contact lens materials using in vitro radiochemical experiments. METHODS:Etafilcon A, senofilcon A and balafilcon A contact lens materials were i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682636
更新日期:2012-09-01 00:00:00
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journal_title:Current eye research
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更新日期:2016-05-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:To detect the immunohistochemical changes in the main lacrimal glands of patients suffering from chronic ocular sequelae of Stevens-Johnson syndrome (SJS). METHODS:Histological sections of biopsies from the lacrimal gland of three chronic SJS patients (mean age, 33 years; 2 males) with severe dry eye disease (...
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pub_type: 杂志文章
doi:10.1080/02713683.2020.1836227
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journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713688908995761
更新日期:1989-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209069168
更新日期:1992-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.5.428.7048
更新日期:1997-05-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713688409003064
更新日期:1984-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608995176
更新日期:1986-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.853194
更新日期:2014-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809047034
更新日期:1988-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/02713680802337397
更新日期:2008-09-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/02713683.2014.932387
更新日期:2015-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020326
更新日期:1991-10-01 00:00:00
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doi:10.1076/ceyr.23.6.406.6969
更新日期:2001-12-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2019-02-01 00:00:00
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doi:10.3109/02713683.2012.694551
更新日期:2012-11-01 00:00:00
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journal_title:Current eye research
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doi:10.3109/02713688409003066
更新日期:1984-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999564
更新日期:1990-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
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doi:10.1076/ceyr.24.6.430.8599
更新日期:2002-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:1993-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409167301
更新日期:1994-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409011750
更新日期:1984-08-01 00:00:00