Ultrastructural immunocytochemical localization of herpes simplex virus (type 1) in trigeminal ganglion neurons.


:Four days after corneal inoculation of mice with herpes simplex (type 1) virus (HSV), infected trigeminal ganglion cells with and without calcitonin gene-related peptide (CGRP) antigenicity were examined by electron microscopy in sections treated with colloidal gold labeled antibodies. Cells that contain CGRP were identified by the dense gold labeling of small vesicles about 100 nm in diameter. Adjacent thin sections were stained using an indirect colloidal gold immunocytochemical technique to reveal HSV-1 antigens. In CGRP-positive neurons, HSV antigens were located over both nuclear and cytoplasmic compartments. HSV label was found over cytoplasmic vesicles that were significantly larger than those labeled with anti-CGRP antisera; the HSV-containing vesicles ranged in profile diameter from less than 170 to greater than 400 nm. There was no overlap in the distribution of the two labels. Thus, for this time period, the organelles involved in transport of the endogenous neuropeptide and HSV appear to remain discrete. Furthermore, there was no significant difference in the distribution of HSV in CGRP-reactive and CGRP-negative trigeminal ganglion cells. Thus, there is no indication of a preferential distribution or limited replication of HSV in CGRP-positive neurons.


Curr Eye Res


Current eye research


LaVail JH,Meade LB,Dawson CR




Has Abstract


1991-01-01 00:00:00








10 Suppl


  • Intentional reactivation of latent ocular herpes infection during BVDU therapy.

    abstract::Sixteen adult New Zealand white rabbits with previously confirmed herpes simplex virus type 1 (HSV-1) infections were stimulated by iontophoresis of 6-hydroxydopamine into the cornea and were followed-up by topical epinephrine treatment to confirm latency. A total of 224 ocular cultures were obtained, of which 73 were...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Trousdale MD,Robin JB,Willey DE,De Clercq E

    更新日期:1987-12-01 00:00:00

  • Swelling pressure and hydration behavior of porcine corneal stroma.

    abstract:PURPOSE:The aim of this study was to characterize swelling pressure-thickness, swelling pressure-hydration and hydration-thickness relations of porcine cornea. METHODS:Mechanical compression tests and free swelling experiments were performed on porcine cornea. A rheometer (DHR-2, TA Instruments) with a thermally contr...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Hatami-Marbini H,Etebu E,Rahimi A

    更新日期:2013-11-01 00:00:00

  • Retinal neurodegeneration in diabetic peripheral neuropathy by optical coherence tomography: a systematic review and meta-analysis.

    abstract::Purpose: The optical coherence tomography (OCT) has been used to evaluate the changes of retinal degeneration in patients with diabetic peripheral neuropathy (DPN) in recent years, but the results of previous studies were controversial. Therefore, systematic review and meta-analysis were performed to evaluate the degr...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Liu B,Wang W,Zhou R,Zeng X,Zhu Z

    更新日期:2021-01-11 00:00:00

  • Optimization of Cultured Human Corneal Endothelial Cell Sheet Transplantation and Post-Operative Sheet Evaluation in a Rabbit Model.

    abstract:PURPOSE:To optimize cultured human corneal endothelial cell (cHCEC) sheet transplantation technique for maintenance of cHCEC viability. MATERIALS AND METHODS:cHCEC sheets cultured on a collagen scaffold were covered with or without Viscoat® and exposed to humidified air in the incubator. cHCEC sheets with or without V...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Yamaguchi M,Shima N,Kimoto M,Ebihara N,Murakami A,Yamagami S

    更新日期:2016-09-01 00:00:00

  • Identification of two of the major phosphorylated polypeptides of the bovine lens utilizing a lens cAMP-dependent protein kinase system.

    abstract::Two of the major in vitro phosphorylated polypeptides of the bovine lens have been identified. Analysis by means of two-dimensional gel electrophoresis (IEF) has demonstrated that the lens phosphorylated 57,000 and 43,000 dalton polypeptides correspond in mobility to purified phosphorylated bovine lens vimentin and ch...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Sredy J,Roy D,Spector A

    更新日期:1984-12-01 00:00:00

  • Protective effect of passive immunization on herpetic retinitis of newborn rabbits.

    abstract::We studied the protective effects of passive immunization with virus specific antibody in newborn rabbits inoculated subcutaneously with type 2 herpes simplex virus (HSV-2). Newborn rabbits given anti-HSV-2 antibody intraperitoneally (IP) on days 0, 2 and 4 post infection had smaller herpetic skin lesions and reduced ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Friedlaender RP,Oh JO,Minasi P,Ohashi Y

    更新日期:1987-01-01 00:00:00

  • The influence of thapsigargin on Na,K-ATPase activity in cultured nonpigmented ciliary epithelial cells.

    abstract::Experiments were conducted to test the influence of thapsigargin on the NaK-ATPase activity of cultured cells (ODM2) derived from human nonpigmented ciliary epithelium. The rate of ouabain-sensitive ATP hydrolysis (Na,K-ATPase activity) was diminished in cells that had been pretreated with thapsigargin then permeabili...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Mito T,Kuwahara S,Delamere NA

    更新日期:1995-08-01 00:00:00

  • A rapid HPLC method for the quantification of GSH and GSSG in ocular lens.

    abstract:UNLABELLED:Purpose. To develop a rapid and accurate method for the quantification of reduced glutathione (GSH) and oxidized glutathione (GSSG) using micro-quantities of ocular lens. Methods. The epithelium, cortex and nucleus of the lens were separated and also the whole lens was homogenized in 3% metaphosphoric acid. ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Liu S,Ansari NH,Wang C,Wang L,Srivastava SK

    更新日期:1996-07-01 00:00:00

  • Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in Pseudoexfoliation.

    abstract:PURPOSE:Pseudoexfoliation (PXF) is a microfibrillopathy involving disordered elastogenesis. Abnormal extracellular matrix (ECM) production underlies the pathophysiology of PXF. The enzyme Lysyl oxidase (LOX) and its isoforms are known to cross-link the elastin and collagen. Though the etiopathogensis of PXF is not well...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Gayathri R,Coral K,Sharmila F,Sripriya S,Sripriya K,Manish P,Shantha B,Ronnie G,Vijaya L,Narayanasamy A

    更新日期:2016-10-01 00:00:00

  • Fluorophotometric Analysis of the Ocular Surface Glycocalyx in Soft Contact Lens Wearers.

    abstract:PURPOSE:Unstable tear film characterized by shorting of tear break-up time (BUT) is associated with discomfort and dryness in contact lens wearers. The glycocalyx is thought to be crucial in maintaining the wettability and lubrication of the ocular surface. We evaluated the ocular surface glycocalyx in soft contact len...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Fukui M,Yamada M,Akune Y,Shigeyasu C,Tsubota K

    更新日期:2016-01-01 00:00:00

  • Lipid synthesis in the bovine lens.

    abstract::Lipid synthesis was investigated in bovine lens in organ culture for 9 hours using 14C acetate as a precursor. The greatest incorporation occurred in phospholipid fraction with phosphatidyl choline being the major phospholipid labeled. Considerable activity was incorporated in the free fatty acid fraction, cholesterol...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Albers-Jackson B,Nga Do A

    更新日期:1987-09-01 00:00:00

  • Interleukin-6 and Diabetic Retinopathy: A Systematic Review and Meta-Analysis.

    abstract:BACKGROUND:Interleukin-6 (IL-6) is produced by a variety of cells involved in inflammation and acts as local intensification signals in pathological processes associated with chronic eye inflammation. This meta-analysis was performed to provide a better understanding of the relationship between IL-6 and diabetic retino...

    journal_title:Current eye research

    pub_type: 杂志文章,meta分析


    authors: Yao Y,Li R,Du J,Long L,Li X,Luo N

    更新日期:2019-05-01 00:00:00

  • Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium.

    abstract::Activity of RNA polymerases I, II and III (distinguished using alpha-amanitin) and activity of DNA polymerases alpha and beta (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carba...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Colley AM,Law ML,Drake LA,Cavanagh HD

    更新日期:1987-03-01 00:00:00

  • Natural course of posterior subcapsular cataract over a short time period.

    abstract::Purpose/aim of the study: To observe and analyze the weekly changes in morphology and size of vacuoles, so called Wedl cells, in eyes with posterior subcapsular cataract (PSC). MATERIALS AND METHODS:Thirty-six eyes of 36 patients with pronounced PSC were included in this prospective study. High-resolution retroillumi...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Neumayer T,Hirnschall N,Georgopoulos M,Findl O

    更新日期:2017-12-01 00:00:00

  • Murine endotoxin-induced uveitis, but not immune complex-induced uveitis, is dependent on the IL-8 receptor homolog.

    abstract:PURPOSE:To determine the roles of the murine interleukin-8 receptor homolog (mIL-8Rh, neutrophil chemokine CXC receptor 2) and macrophage inflammatory protein-1alpha (MIP-1alpha, a CC chemokine) in two eye inflammation models: endotoxin-induced uveitis (EIU) and immune complex-induced uveitis (reverse passive Arthus re...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Brito BE,O'Rourke LM,Pan Y,Huang X,Park JM,Zamora D,Cook DN,Planck SR,Rosenbaum JT

    更新日期:1999-07-01 00:00:00

  • Computerised densitometry of red-free retinal photographs correlated with automatic perimetry.

    abstract::An image analyser installed in a 16 bit personal computer was used to measure grey levels in red free photographs of normal and glaucomatous eyes. Regional photographic image intensity and density were correlated with Humphrey Field Analyser results for corresponding areas of visual field. Two methods for standardisin...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Cooper RL,Eikelboom RH,Barry C

    更新日期:1988-08-01 00:00:00

  • Successful Consecutive Expansion of Limbal Explants Using a Biosafe Culture Medium under Feeder Layer-Free Conditions.

    abstract:PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: López-Paniagua M,Nieto-Miguel T,de la Mata A,Galindo S,Herreras JM,Corrales RM,Calonge M

    更新日期:2017-05-01 00:00:00

  • Characterization of alpha 2-adrenergic receptors, negatively coupled to adenylate cyclase, in rabbit ciliary processes.

    abstract::Inhibition of ciliary process adenylate cyclase was studied on rabbit membrane preparations. When considered individually, epinephrine, GTP and NaCl did not inhibit adenylate cyclase activity. On the other hand, when present together, epinephrine, GTP (10(-5) M) and NaCl (200 mM) acted synergistically to cause a 27% i...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Kintz P,Himber J,de Burlet G,Andermann G

    更新日期:1988-03-01 00:00:00

  • The effect of nitric oxide synthase inhibitor on form-deprivation myopia.

    abstract:PURPOSE:Form-deprivation myopia (FDM) is believed to result principally from actions of substances that modulate information processing in the retina. We used a chick model to investigate what role nitric oxide (NO), a gaseous neuromodulator, might play in the development of FDM. METHODS:We injected different concentr...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Fujikado T,Kawasaki Y,Fujii J,Taniguchi N,Okada M,Suzuki A,Ohmi G,Tano Y

    更新日期:1997-10-01 00:00:00

  • Effects of serotonergic compounds on aqueous humor dynamics in monkeys.

    abstract:PURPOSE:The effects of several serotonergic agonists on aqueous humor formation (AHF), total outflow facility (OF) and intraocular pressure (IOP) were investigated in living cynomolgus monkeys. METHODS:We determined the effect of a single topical unilateral 300 microg or 3 mg dose of the 5-HT agonists serotonin, 5-car...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Gabelt BT,Millar CJ,Kiland JA,Peterson JA,Seeman JL,Kaufman PL

    更新日期:2001-08-01 00:00:00

  • Morphological evidence for the transfer of anionic macromolecules from the interior of the eye to the blood stream.

    abstract::We have either introduced into the vitreous space or perfused through the anterior chamber of macaque monkey eyes two anionic tracers, anionic ferritin (AF) and horseradish peroxidase (HRP) and a cationic probe, cationic ferritin (CF). We have observed that the anionic molecules, but not the cationic one, are transpor...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Raviola G,Butler JM

    更新日期:1985-04-01 00:00:00

  • Changes in lens protein in concentric fractions from individual normal human lenses.

    abstract::The water soluble (WS), urea soluble (US) and urea insoluble (UI) fractions from individual human lenses 1.8 to 65 years of age were isolated from concentric fiber layers. In lenses younger than 19 years, a uniform distribution in the amount of WS, US and UI fractions was found throughout the entire lens. These fracti...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Li LK,Roy D,Spector A

    更新日期:1986-02-01 00:00:00

  • Rebuilding the Retina: Prospects for Müller Glial-mediated Self-repair.

    abstract::Retinal degeneration is a leading cause of untreatable blindness in the industrialised world. It is typically irreversible and there are few curative treatments available. The use of stem cells to generate new retinal neurons for transplantation purposes has received significant interest in recent years and is beginni...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Langhe R,Pearson RA

    更新日期:2020-03-01 00:00:00

  • Tear Production Levels and Dry Eye Disease Severity in a Large Norwegian Cohort.

    abstract:PURPOSE:To determine if the Schirmer I test (without anesthesia) cut-off value is a predictor of dry eye severity in a large Norwegian cohort of dry eye disease (DED) patients, which are grouped into six levels of tear production. METHODS:Patients (n = 1090) with DED of different etiologies received an extensive dry e...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Yazdani M,Chen X,Tashbayev B,Utheim ØA,Ræder S,Lagali N,Stojanovic A,Dartt DA,Utheim TP

    更新日期:2018-12-01 00:00:00

  • Quantifying retinal irradiance levels in light damage experiments.

    abstract::From knowledge of the optical parameters of experimental animal eyes and radiometric parameters of the light source, it is possible to provide accurate retinal irradiances (dose rates). Examples are given for the rat, cat, monkey and other animals of interest. Fluorescent lamps and incandescent lamp radiances are prov...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Sliney DH

    更新日期:1984-01-01 00:00:00

  • Regulation of human corneal epithelial mucins by rebamipide.

    abstract:PURPOSE:Membrane-associated mucins (MAMs) play important roles in barrier function and tear stability, and their expression on the ocular surface is altered in dry eye disease. Rebamipide is a mucin secretagogue that promotes the production of mucin-like glycoproteins in human corneal epithelial (HCE) cells. However, t...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Itoh S,Itoh K,Shinohara H

    更新日期:2014-02-01 00:00:00

  • An in vitro model of leukocyte mediated injury to the corneal epithelium.

    abstract::To enhance efforts directed at unraveling the role and mechanisms of leukocytes in mediating injury to corneal epithelium, an isolated bovine corneal cup was developed and evaluated. Bovine peripheral leukocytes and lysates were added to the corneal epithelial surface of isolated cornea for various periods after which...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Elgebaly SA,Gillies C,Forouhar F,Hashem M,Baddour M,O'Rourke J,Kreutzer DL

    更新日期:1985-01-01 00:00:00

  • Polymorphonuclear leukocyte cells and elastase in tears.

    abstract:PURPOSE:To characterize the effects that mode of sampling and overnight eye closure have on the nature of caseinolytic activity recovered in tear fluid. METHODS:Reflex, open and closed (R, O and C) eye tear fluids were collected by microcapillary tubes or from the inferior formix by Schirmer strip. Microcapillary coll...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Sakata M,Sack RA,Sathe S,Holden B,Beaton AR

    更新日期:1997-08-01 00:00:00

  • Prevalence and risk factors of diabetic retinopathy in subjects with suboptimal glycemic, blood pressure and lipid control. Sankara Nethralaya Diabetic Retinopathy Epidemiology and Molecular Genetic Study (SN-DREAMS, Report 33).

    abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Raman R,Gupta A,Kulothungan V,Sharma T

    更新日期:2012-06-01 00:00:00

  • Effect of human RAD50 gene therapy on glaucoma filtering surgery in rabbit eye.

    abstract:PURPOSE:To investigate the antiproliferative effects of the subconjunctival injection of human RAD50 (hRAD50) on fibroblasts after glaucoma filtering surgery. METHODS:After glaucoma filtering surgery in normal rabbit eye, the subconjunctival injection of hRAD50 was performed. Morphologic changes in the subconjunctival...

    journal_title:Current eye research

    pub_type: 杂志文章


    authors: Yoon KC,Yang KJ,Seo JS,Lee SE,Heo T,Chun BJ,Jung S,Kim KK,Ahn KY

    更新日期:2004-03-01 00:00:00