Ultrastructural immunocytochemical localization of herpes simplex virus (type 1) in trigeminal ganglion neurons.

Abstract:

:Four days after corneal inoculation of mice with herpes simplex (type 1) virus (HSV), infected trigeminal ganglion cells with and without calcitonin gene-related peptide (CGRP) antigenicity were examined by electron microscopy in sections treated with colloidal gold labeled antibodies. Cells that contain CGRP were identified by the dense gold labeling of small vesicles about 100 nm in diameter. Adjacent thin sections were stained using an indirect colloidal gold immunocytochemical technique to reveal HSV-1 antigens. In CGRP-positive neurons, HSV antigens were located over both nuclear and cytoplasmic compartments. HSV label was found over cytoplasmic vesicles that were significantly larger than those labeled with anti-CGRP antisera; the HSV-containing vesicles ranged in profile diameter from less than 170 to greater than 400 nm. There was no overlap in the distribution of the two labels. Thus, for this time period, the organelles involved in transport of the endogenous neuropeptide and HSV appear to remain discrete. Furthermore, there was no significant difference in the distribution of HSV in CGRP-reactive and CGRP-negative trigeminal ganglion cells. Thus, there is no indication of a preferential distribution or limited replication of HSV in CGRP-positive neurons.

journal_name

Curr Eye Res

journal_title

Current eye research

authors

LaVail JH,Meade LB,Dawson CR

doi

10.3109/02713689109020354

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

23-9

eissn

0271-3683

issn

1460-2202

journal_volume

10 Suppl

pub_type

杂志文章
  • Wearing Swimming Goggles Reduces Central Corneal Thickness and Anterior Chamber Angle, and Increases Intraocular Pressure.

    abstract::Purpose/Aim: To assess the changes in anterior eye segment biometrics and intraocular pressure during and after wearing swimming goggles.Materials and methods: Anterior segment biometry and intraocular pressure were assessed in 20 healthy young adults (22.2 ± 4.7 years) during the wearing of a drilled swimming goggle....

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2019.1662056

    authors: Jiménez R,Molina R,García JA,Redondo B,Vera J

    更新日期:2020-05-01 00:00:00

  • Quantification of Retinal Microvascular Density Using Optic Coherence Tomography Angiography in Primary Angle Closure Disease.

    abstract::Purpose: To evaluate the microvascular density of the peripapillary and parafoveal areas of the eyes with primary angle closure disease (PACD) using optical coherence tomography angiography (OCTA). Materials and methods: An observational study was conducted with 76 PACD patients (29 eyes with primary angle closure sus...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2020.1849728

    authors: Wang X,Chen J,Kong X,Sun X

    更新日期:2020-11-26 00:00:00

  • Effect of Isolated Oblique Muscle Weakening Procedures on Horizontal Deviation in A- and V-pattern Exotropia.

    abstract::Purpose: To evaluate the effect of isolated oblique muscle weakening on horizontal deviation in patients with A- and V-pattern exotropia.Methods: The medical records of patients who underwent bilateral oblique muscle weakening for A- and V-pattern exotropia associated oblique muscle overaction between 2011 and 2018 we...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2019.1660795

    authors: Wang X,Zhang W,Liu L

    更新日期:2020-02-01 00:00:00

  • Experimental preretinal neovascularization by laser-induced venous thrombosis in rats.

    abstract:PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After a...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.16.1.26.5116

    authors: Saito Y,Park L,Skolik SA,Alfaro DV,Chaudhry NA,Barnstable CJ,Liggett PE

    更新日期:1997-01-01 00:00:00

  • Age-dependent effect of UV light in abnormal alpha neoprotein formation in the lens.

    abstract::The aim of this study was to determine whether or not alpha neoprotein formation can occur in the lens in response to a photolytic process. Alpha crystallin from young calf and aged bovine lenses was irradiated for up to 22 hours with UV light. After cortical alpha crystallin had been irradiated, it completely preserv...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688809001882

    authors: Hibino K,Du J,Dillon J,Malinowski K

    更新日期:1988-11-01 00:00:00

  • Real-time confocal microscopic observations on human corneal nerves and wound healing after excimer laser photorefractive keratectomy.

    abstract:PURPOSE:Corneal wound healing after excimer laser photorefractive keratectomy (PRK) passes through a series of characteristic stages which have earlier been defined by means of histological, histochemical, and biochemical approaches. We investigated the potential of confocal microscopy to verify morphological changes i...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.16.7.640.5058

    authors: Linna T,Tervo T

    更新日期:1997-07-01 00:00:00

  • The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells in vitro.

    abstract:PURPOSE:Maintaining mature and viable retinal pigment epithelial cells (RPE) in vitro has proven challenging. Investigating compounds that can promote RPE-viability and maturation is motivated by RPE transplantation research, the quest to understand RPE physiology, and a desire to modulate RPE in pathological states. W...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2020.1809001

    authors: Khan AZ,Utheim TP,Moe MC,Aass HCD,Sapkota D,Vallenari EM,Eidet JR

    更新日期:2020-09-01 00:00:00

  • Immunological characterization of calf lens gamma-crystallins, separated by preparative isoelectric focusing.

    abstract::Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological componen...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688109001869

    authors: Bours J,Vornhagen R,Herlt M,Rink H

    更新日期:1981-01-01 00:00:00

  • Neurotransmitters in the retina.

    abstract::Processing of visual information within the retina depends in large measure upon a complement of chemical neurotransmitters which are released at synaptic contacts between individual neurons. Numerous investigators have participated in the identification of many of these transmitters and their assignment to specific n...

    journal_title:Current eye research

    pub_type: 杂志文章,评审

    doi:10.3109/02713689609003465

    authors: Pourcho RG

    更新日期:1996-07-01 00:00:00

  • Corneal autofluorescence in relation to permeability of the blood-aqueous barrier in diabetic patients with clinically significant macular edema and in an age-matched control group.

    abstract:PURPOSE:Corneal autofluorescence is related to advanced glycation end products formed by glucose that reaches the cornea via the aqueous humour. The aim of the study was to examine the influence on autofluorescence of changes in permeability of the blood aqueous barrier. METHODS:Corneal autofluorescence was measured i...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1076/ceyr.26.4.307.15433

    authors: Kessel L,Moldow B,van Best JA,Sander B

    更新日期:2003-05-01 00:00:00

  • The cross-species A3 adenosine-receptor antagonist MRS 1292 inhibits adenosine-triggered human nonpigmented ciliary epithelial cell fluid release and reduces mouse intraocular pressure.

    abstract:PURPOSE:Antagonists to A3 adenosine receptors (ARs) lower mouse intraocular pressure (IOP), but extension to humans is limited by species variability. We tested whether the specific A3AR antagonist MRS 1292, designed to cross species, mimicks the effects of other A3AR antagonists on cultured human nonpigmented ciliary ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713680590953147

    authors: Yang H,Avila MY,Peterson-Yantorno K,Coca-Prados M,Stone RA,Jacobson KA,Civan MM

    更新日期:2005-09-01 00:00:00

  • Sodium/potassium ATPase in normal and cataractous human lenses.

    abstract::Na/K ATPase activity has been determined in normal and cataractous human lenses using labeled ATP as a substrate. The enzyme is distributed throughout the lens with approximately 1/3 of the total activity in the capsule-epithelium and 1/2 of the activity in the cortex. Furthermore, the activity of Na/K ATPase decrease...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688209000778

    authors: Kobatashi S,Roy D,Spector A

    更新日期:1982-01-01 00:00:00

  • 24-hour intraocular pressure and blood pressure levels with latanoprost/timolol fixed combination versus timolol.

    abstract:PURPOSE:To evaluate 24-hr intraocular pressure (IOP) and blood pressure (BP) with timolol or latanoprost/timolol fixed combination (LTFC). METHODS:Patients with primary open-angle glaucoma or ocular hypertension with normal blood pressure were randomized to LTFC, dosed each evening, or timolol dosed twice daily in a c...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验

    doi:10.1080/02713680902850075

    authors: Konstas AG,Pikilidou MI,Tsironi S,Mikropoulos D,Kozobolis VP,Sarafidis PA,Lasaridis AN,Nelson LA,Stewart WC

    更新日期:2009-05-01 00:00:00

  • Prostaglandins increase proMMP-1 and proMMP-3 secretion by human ciliary smooth muscle cells.

    abstract:PURPOSE:The mechanism by which prostaglandin(PG)F2 alpha increases uveoscleral outflow and lowers intraocular pressure in primates is not known. In cultured human ciliary muscle cells, PGF2 alpha induces the expression of the protooncogene c-fos which is known to induce the transcription of genes such as matrix metallo...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713689609017628

    authors: Lindsey JD,Kashiwagi K,Boyle D,Kashiwagi F,Firestein GS,Weinreb RN

    更新日期:1996-08-01 00:00:00

  • Detection of Pro- and Antiangiogenic Factors in the Human Sclera.

    abstract:PURPOSE:Avascular tissues can be used to identify antilymph- or antihemangiogenic factors. The human sclera-the outer covering layer of the eye, lacks lymphatic vessels and contains only a superficial network of blood vessels and was used here to identify endogenous antiangiogenic factors. METHODS:Expression levels of...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2018.1540704

    authors: Schlereth SL,Karlstetter M,Hos D,Matthaei M,Cursiefen C,Heindl LM

    更新日期:2019-02-01 00:00:00

  • Liquid chromatographic determination of uric acid and ascorbic acid in rat retinae after ophthalmic artery and optic nerve ligation.

    abstract::Change of urate and ascorbate levels in retinae of rat left eyes after ophthalmic artery and optic nerve ligation was studied by reversed-phase high-performance liquid chromatography with electrochemical detection. The right eyes were used for sham operation. We found that uric acid levels in the retinae of the left e...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688608996369

    authors: Iriyama K,Yoshiura M,Iwamoto T,Kamada Y,Nozawa H,Mizuno A,Matsuzaki H

    更新日期:1986-07-01 00:00:00

  • Modulators of cyclic AMP in monolayer cultures of Y-79 retinoblastoma cells: partial characterization of the response with VIP and glucagon.

    abstract::VIP markedly stimulates intracellular cAMP accumulation in the human retinoblastoma Y-79 cell line. cAMP increased about 5-fold above the basal level with 10(-8)M VIP and reached a maximum level (about 70-fold increase) with 2 X 10(-6)M VIP. Glucagon at 6 X 10(-8)M significantly increased cAMP accumulation with a maxi...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688408997218

    authors: Kyritsis A,Koh SW,Chader GJ

    更新日期:1984-02-01 00:00:00

  • Comparison of 2-methoxyestradiol and methotrexate effects on non-Hodgkin's B-cell lymphoma.

    abstract:PURPOSE:Methotrexate (MTX) is the most commonly used chemotherapeutic agent to treat primary central nervous system lymphoma (PCNSL) and intraocular lymphoma (IOL). 2-methoxyestradiol (2ME2) is a potent antitumor and anti-angiogenesis agent which, unlike other cytotoxic drugs, has minimal toxicity. In this study, anti-...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713680701473244

    authors: Park J,Franco RS,Augsburger JJ,Banerjee RK

    更新日期:2007-07-01 00:00:00

  • Characterization of human meibum lipid using raman spectroscopy.

    abstract:PURPOSE:Raman spectroscopy could potentially be used as a diagnostic tool for the detection of meibomian gland dysfunction by measuring the composition, conformation, and amount of meibum lipid on the lid margin. Toward this goal and to test our studies of meibum lipid using infrared spectroscopy, we measured the Raman...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713680903122029

    authors: Oshima Y,Sato H,Zaghloul A,Foulks GN,Yappert MC,Borchman D

    更新日期:2009-10-01 00:00:00

  • VIP modulation of cultured glial cells of the rat retina.

    abstract::Cultured retinal glial cells from the rat are responsive to modulation by vasoactive intestinal peptide (VIP). VIP (1 X 10(-6) M) elevated the intracellular cyclic AMP concentration from the basal level of (4.4-11.1) p mole/mg protein to (354-440) p mole/mg protein in three minutes at 25 degrees C. The half-maximal co...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688909000045

    authors: Koh SW,Roberge FG

    更新日期:1989-11-01 00:00:00

  • Production and specificity of monoclonal antibodies to retinal S antigen.

    abstract::Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale cultures and as ascites in mice. The specificity of the antibo...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688409000800

    authors: Faure JP,Mirshahi M,Dorey C,Thillaye B,de Kozak Y,Boucheix C

    更新日期:1984-06-01 00:00:00

  • Collared silicone punctal plugs compared to intracanalicular plugs for the treatment of dry eye.

    abstract:AIM:To compare collared silicone punctal plugs to intracanalicular SmartPlugs for the treatment of moderate to severe dry eye. MATERIALS AND METHODS:In this prospective, randomized, single blind, clinical study, 30 patients (60 eyes) who had been diagnosed with moderate to severe dry eye syndrome were enrolled. Study ...

    journal_title:Current eye research

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/02713683.2013.765487

    authors: Rabensteiner DF,Boldin I,Klein A,Horwath-Winter J

    更新日期:2013-05-01 00:00:00

  • Vitreous Hyper-Reflective Dots in Optical Coherence Tomography and Retinal Tear in Patients with Acute Posterior Vitreous Detachment.

    abstract:PURPOSE:To report the presence of hyper-reflective dots in the vitreous cavity using spectral-domain optical coherence tomography (SD-OCT) in patients with acute symptomatic posterior vitreous detachment (PVD) and investigate their association with the presence of retinal tear. METHODS:The medical records of 77 patien...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2017.1289226

    authors: Oh JH,Oh J,Roh HC

    更新日期:2017-08-01 00:00:00

  • Anterior Migration of Triamcinolone Acetonide after Posterior Subtenon Injection for Macular Edema Predisposes to Intraocular Pressure Elevation.

    abstract:PURPOSE:To evaluate the effect of anterior migration of triamcinolone acetonide on intraocular pressure (IOP) elevation after posterior subtenon injection of triamcinolone acetonide (PSTA) for macular edema. METHODS:One hundred and ten eyes from 89 patients who received PSTA for macular edema were prospectively enroll...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2020.1826979

    authors: Yang YH,Hsu WC,Hsieh YT

    更新日期:2020-09-27 00:00:00

  • Role of Nitric Oxide in the Development of Cataract Formation in CdCl2-induced Hypertensive Animals.

    abstract:PURPOSE:Previously we established a strong association of systemic hypertension with cataract formation. In the present study, we investigated the role of nitric oxide (NO) in the development of cataract formation in CdCl2-induced hypertensive animals. MATERIALS AND METHODS:Hypertension was induced in male albino rats...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2018.1501490

    authors: Yadav A,Choudhary R,Bodakhe SH

    更新日期:2018-12-01 00:00:00

  • Spectral-Domain Optical Coherence Tomography for Determination of Retinal Thickness in Pediatric Patients with Mild-To-Moderate Chronic Kidney Disease: A Cross-Sectional Study.

    abstract:OBJECTIVE:Children with chronic kidney diseases (CKD) are at risk for neurological diseases at early adulthood. Spectral-domain optical coherence tomography (SD-OCT) of the retina is especially suitable for determination of intraretinal layer thickness. We wonder whether retinal thinning is already present in pediatric...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2018.1522649

    authors: Prakasam RK,Götze A,von Keyserlingk S,Jünemann A,Röhlig M,Stachs O,Fischer DC

    更新日期:2019-02-01 00:00:00

  • Trabecular meshwork recovery after phagocytic challenge.

    abstract::The short and long term response of the trabecular meshwork to a phagocytic challenge and the response of the meshwork to different types of foreign particles was studied by injecting one eye of 25 adult cats with a phagocytic agent (zymosan, blood, or latex microspheres) while the fellow eye received a control soluti...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688909000037

    authors: Johnson DH,Richardson TM,Epstein DL

    更新日期:1989-11-01 00:00:00

  • An in vivo model for dissociating alpha 2-and DA2-adrenoceptor activity in an ocular adnexa: utility of the cat nictitating membrane preparation.

    abstract::Recent reports from this laboratory indicate that dopamine (DA2) agonists can lower intraocular pressure (IOP) in rabbits, monkeys and rats. This communication suggests that the cat nictitating membrane (CNM) preparation is a useful model for localizing the site(s) and mechanism(s) of action of dopamine (DA2) agonists...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688409007415

    authors: Potter DE,Burke JA

    更新日期:1984-11-01 00:00:00

  • Successful Consecutive Expansion of Limbal Explants Using a Biosafe Culture Medium under Feeder Layer-Free Conditions.

    abstract:PURPOSE:Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost ...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.1080/02713683.2016.1250278

    authors: López-Paniagua M,Nieto-Miguel T,de la Mata A,Galindo S,Herreras JM,Corrales RM,Calonge M

    更新日期:2017-05-01 00:00:00

  • Intracorneal lesions produced with focused ultrasound.

    abstract::Focused ultrasound concentrates a large amount of ultrasonic energy into a small focal zone. We have used new techniques to develop a transducer that creates intracorneal, thermally induced lesions in rabbit eyes in vitro. Histologic study shows that these lesions are confined to the stroma and that the procedure caus...

    journal_title:Current eye research

    pub_type: 杂志文章

    doi:10.3109/02713688209000777

    authors: Mark DB,Beuerman R

    更新日期:1982-01-01 00:00:00