Abstract:
PURPOSE:To examine whether transforming growth factor-beta 2 (TGF-beta 2) induces the expression of alpha-smooth muscle actin (SMA), a biochemical marker of myofibroblasts, in cultured bovine retinal pigment epithelial (RPE) cells. METHODS:Bovine RPE cells were cultured in F-12 nutrient mixture supplemented with 5% fetal bovine serum (FBS), with or without TGF-beta 2 (0.01-10 ng/ml) for 6 days. During this culture period, cells did not reach a confluence. Alpha-SMA was detected immunocytochemically with a mouse monoclonal antibody, and the ratio of the number of alpha-SMA positive cells to the total number of cells was calculated. RESULTS:About 10% of the cells in control cultures with only FBS were positive for alpha-SMA. TGF-beta 2 increased the ratio of positive cells dose-dependently (p < 0.0001), while a neutralizing antibody against TGF-beta 2 blocked this effect. CONCLUSIONS:TGF-beta 2 induced expression of alpha-SMA in bovine RPE cells.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Kurosaka D,Muraki Y,Inoue M,Katsura Hdoi
10.3109/02713689608995147subject
Has Abstractpub_date
1996-11-01 00:00:00pages
1144-7issue
11eissn
0271-3683issn
1460-2202journal_volume
15pub_type
杂志文章abstract:PURPOSE:It has been demonstrated that chick eye growth compensates for defocus imposed by spectacle lenses: the eye elongates in response to hyperopic defocus imposed by negative lenses and slows its elongation in response to myopic defocus imposed by positive lenses. We ask whether the synthesis of scleral extracellul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.4.320.10697
更新日期:1997-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.4.338.5307
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001859
更新日期:1981-01-01 00:00:00
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journal_title:Current eye research
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doi:10.1080/02713683.2019.1606252
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.1.23.9966
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2013-05-01 00:00:00
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更新日期:2017-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609020047
更新日期:1986-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.7.710.5064
更新日期:1997-07-01 00:00:00
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更新日期:2017-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017628
更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1080/02713683.2016.1255335
更新日期:2017-06-01 00:00:00
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doi:10.1080/02713683.2018.1522649
更新日期:2019-02-01 00:00:00
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更新日期:1995-11-01 00:00:00
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doi:10.1080/02713683.2016.1259421
更新日期:2017-06-01 00:00:00
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更新日期:1994-03-01 00:00:00
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更新日期:2005-02-01 00:00:00
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更新日期:2012-04-01 00:00:00
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更新日期:1992-01-01 00:00:00
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更新日期:1990-01-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00