Abstract:
PURPOSE:Chloride efflux plays an important role in aqueous humor production. Chloride currents have been described in bovine non-pigmented ciliary epithelium (NPE), in transformed cultured human NPE, and in bovine volume-activated chloride channels described in the latter. It is the basolateral membranes of the NPE of ocular ciliary processes that comprise the exit pathway for the process of aqueous secretion performed by the double syncytial layer of ciliary epithelium, however. Therefore, we studied both cell-attached, and, excised, inside-out patches from basolateral membranes of the NPE. METHODS:Cell-attached and cell-free excised patches were formed from the basolateral membranes of NPE and single channel currents recorded with a Dagan 3900A patch clamp amplifier. RESULTS:A low conductance channel of 14 pS was observed and recorded in 30% of cell attached patches and in 35% of excised inside out patches under symmetrical conditions (160 mM chloride). This channel displayed a nearly linear current-voltage relationship, with an open probability that was not voltage-dependent. The channel was chloride-selective: N-methyl-D-glucamine (NMDG) used as cation did not alter the current profile nor the reversal protein. Further, with inside-out patches, the reversal potential was close to zero (0.3 +/- 0.5 mV (10) in symmetrical (160 mV) chloride, but shifted to -32.3 +/- 0.5 mV (5) when the concentration of chloride in the bathing solution was reduced to 40 mM while the recording pipette was held at 160 mM. This value approaches the theoretical equilibrium potential of chloride for these conditions. The channel anion permeability sequence was: I- > NO3- > or = Br- > Cl- > gluconate- approximately equal to aspartate-. Three different chloride-channel blockers inhibited the channel activity. CONCLUSION:A low conductance channel, selective for chloride, and, modulated by beta adrenergic and VIP stimulation, based on it sensitivity to exogenously added cAMP, ATP and the catalytic subunit of PKA, is present in the exit basolateral membranes of rabbit NPE and contributes to the process of aqueous humor formation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Chen S,Sears Mdoi
10.1076/ceyr.16.7.710.5064subject
Has Abstractpub_date
1997-07-01 00:00:00pages
710-8issue
7eissn
0271-3683issn
1460-2202journal_volume
16pub_type
杂志文章abstract:PURPOSE:The purpose of the current study is to assess changes in the expression of Acid-Sensing Ion Channel (ASIC)1a and ASIC2 in retinal ganglion cells (RGCs) after retinal ischemia and reperfusion (I/R) injury and to test if inhibition of ASIC1a provides RGC neuroprotection. METHODS:Transient ischemia was induced in...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1454478
更新日期:2018-07-01 00:00:00
abstract:PURPOSE:Due to a possible link in humans between atherosclerosis, a high-fat diet, and the development of age-related retinal degenerations, we investigated retinal changes with age and diet in high-fat-fed C57BL/6 mice, a mouse model of human atherosclerosis. METHODS:We fed C57BL/6J mice either a normal chow diet or ...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408995780
更新日期:1994-03-01 00:00:00
abstract:PURPOSE:Mechanical stimulation induces intercellular Ca(2 +) waves in the corneal endothelium. The extent of the wave propagation is dependent on the activity of gap junctions, hemichannels, and ectonucleotidases. To further establish the use of a cell culture model to investigate intercellular communication, in this s...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680902913022
更新日期:2009-06-01 00:00:00
abstract:PURPOSE:Acanthamoeba keratitis is a sight-threatening corneal infection. It is known that: (i) more amoebae bind to the surface of injured corneas than to the normal corneal surface and (ii) mannose-containing glycoproteins (GPs) possess binding sites for Acanthamoeba. The present study was undertaken to determine whet...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Calpains are calcium-activated proteinases which have been implicated in tissue differentiation and degeneration. The aims of the present study were: (1) to determine the relationship between postnatal age and calpain activity in the rat retina; (2) to test if calpain activity was aberrant in the RCS retina at differe...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508998502
更新日期:1995-08-01 00:00:00
abstract::Papavarine, cholera toxin, and isoproterenol each elevated cyclic AMP in cultured rat retinal pigment epithelium and reduced the phagocytosis of isolated rod outer segments (38-66% of controls) during a 4 hour incubation. In addition, cultured pigment epithelium was preincubated with 25 mM exogenous cyclic AMP for two...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608995161
更新日期:1986-01-01 00:00:00
abstract::Transforming growth factor-beta (TGF-beta), a multifunctional cytokine which has been identified in normal and inflamed ocular fluids, may play a role in the evolution of inflammatory ocular lesions. In this study we utilized a rabbit model of LPS-induced uveitis to determine if exogenous TGF-beta 2 could alter its co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017616
更新日期:1996-01-01 00:00:00
abstract::Two alternative methods for the study of the lens cytoskeleton are described which serve to overcome some of the difficulties imparted by the unique biology of the lens. The first technique involves rapid freezing, thick sectioning, and selective extraction and/or fixation of the lens section. This approach offers sev...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997582
更新日期:1990-11-01 00:00:00
abstract:PURPOSE:We performed nailfold capillary microscopy to explore microvasculature abnormalities in uveitis overall and uveitis stratified in various ways. METHODS:This was a cross-sectional, case-control, observational study. One hundred and seven uveitis patients and 130 control subjects were included. We used a JH-1004...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1496265
更新日期:2018-11-01 00:00:00
abstract::Prorenin (PR) was localized by electron microscopic (EM) immunostaining of cryo-ultramicrotomy sections of human ciliary body and correlated with light microscopic immunostaining. Both layers of the ciliary epithelium contained the prohormone. However, density was much higher in the adjacent extracellular spaces, part...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309020401
更新日期:1993-10-01 00:00:00
abstract:PURPOSE:The purpose of this work is to describe and review the technology of plasma rich in growth factors (PRGF), a novel blood derivative product, in the treatment of ocular surface disorders. METHODS:To demonstrate the importance of this technology in the treatment of ocular pathologies, a thorough review of the pr...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713683.2015.1104362
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:Both HSV-1 and HSV-2 express LAT during viral latency. Previous studies indicated that deletion of either LAT impairs viral reactivation from latency, but that the HSV-1 LAT confers the ability to reactivate efficiently from trigeminal ganglia onto HSV-2. We sought to determine whether the HSV-2 LAT could funct...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.3.219.14896
更新日期:2003-03-01 00:00:00
abstract:PURPOSE:To assess the incidence of cell proliferation and apoptosis in epiretinal membranes from eyes with proliferative vitreoretinopathy (PVR), proliferative diabetic retinopathy (PDR), and macular pucker (MP) and to further investigate the potential involvement of key executors of apoptosis. METHODS:Epiretinal memb...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680590956306
更新日期:2005-05-01 00:00:00
abstract::The acyclic nucleoside phosphonate analogue PMEA [9-(2-phosphonylmethoxyethyl)adenine] is a broad spectrum antiviral agent effective against DNA viruses and retroviruses. It is particularly active against the human immunodeficiency virus and, like other phosphonylmethoxyalkyl derivatives, it also inhibits HSV-1, TK- H...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020370
更新日期:1991-01-01 00:00:00
abstract:PURPOSE/AIMS:To evaluate the prognostic factors for the best corrected visual acuity (BCVA) and central subfield macular thickness (CSMT) after vitrectomy with internal limiting membrane (ILM) peeling for diabetic macular edema. MATERIALS AND METHODS:A total of 44 eyes of 35 patients who had undergone vitrectomy with ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.820327
更新日期:2013-12-01 00:00:00
abstract::Free radicals and oxidative damage play roles in aging and age-related ocular diseases such as cataracts, so defensive mechanisms become important factors for protection. Because N-acetylation is involved in a wide variety of detoxification processes, this study was conducted to examine the relationship between the ac...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680591003799
更新日期:2005-10-01 00:00:00
abstract::Although patients with diabetic retinopathy have been reported to have elevated vitreal IGF-I levels, it is not known whether diabetes also affects the levels of vitreal IGF binding proteins (IGFBPs) which control IGF's bioavailability. To address this issue, vitreal IGFBP levels were assayed in human diabetics, rats ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408999886
更新日期:1994-07-01 00:00:00
abstract::Interleukin 4 (IL-4) may play a role in the development of allergic disease. We questioned whether IL-4 related phenomenon occurred on the surface of the conjunctiva during allergic conjunctivitis. Ten patients with cedar pollen-allergic conjunctivitis and 10 patients with postsurgical conjunctivitis were enrolled in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608997415
更新日期:1996-02-01 00:00:00
abstract::The new HERPCHEK (Dupont, No. Billerica, MA) enzyme immunosorbent assay (EIA) was used in a double-blind clinical study for rapid and specific detection of ocular herpes simplex virus (HSV) infection. This 4-hour assay can be used to demonstrate conclusively the presence of HSV antigen without culture and thereby rapi...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713688809033194
更新日期:1988-07-01 00:00:00
abstract::Inoculation of herpes simplex virus Type 1 into the anterior chamber of BALB/c mice induces a characteristic ocular infection involving primarily the anterior segment of virus-injected eyes and the retina of contralateral uninjected eyes. In the BALB/c strain, which is susceptible to herpetic retinitis, anti-HSV delay...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688808995741
更新日期:1988-02-01 00:00:00
abstract:PURPOSE:To examine the effects of calcitonin gene-related peptide (CGRP) on intraocular pressure (IOP). METHODS:IOP was periodically measured in rabbits treated with intravitreal injection (20 ael) of either: 1) CGRP (10(-4) approximately 10(-7) M) into one eye; 2) CGRP (10(-4) or 10(-6) M) into both eyes 30 min after...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.5.432.5291
更新日期:1999-11-01 00:00:00
abstract:PURPOSE:To determine by immunohistochemistry the distribution of different nitric oxide synthases (NOS) isoforms in the porcine eye. METHODS:By light microscopy (immunofluorescence), porcine ocular tissues were investigated using monoclonal antibodies against neuronal NOS (nNOS; NOS I), endothelial NOS (ecNOS; NOS III...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.5.375.5355
更新日期:1999-05-01 00:00:00
abstract:PURPOSE:We located immunohistochemically the matrix metalloproteinases (MMP) -1, -2, -3 and -9 and the tissue inhibitors of matrix metalloproteinases (TIMP) -1 and -2 in the fibrous capsule of patients with intraocular lenses (IOLs). METHODS:During vitreoretinal surgery in 10 patients we obtained post-cataract surgery...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.21.6.962.6989
更新日期:2000-12-01 00:00:00
abstract::Purpose: To evaluate the efficacy of polytetrafluoroethylene/polylactide-co-glycolide (PTFE/PLGA) laminate containing sustained-release thalidomide for delayed adjustable strabismus surgery. Methods: This is a prospective, masked-observer, controlled study using 50 eyes of 25 rabbits. After superior rectus muscle (SRM...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1591461
更新日期:2019-07-01 00:00:00
abstract:PURPOSE:To investigate orbital biomechanical properties in patients with thyroid orbitopathy and in age- and gender-matched healthy subjects using the Corvis-ST (CST, Oculus Wetzlar, Germany). METHODS:The CST allows a non-contact tonometry with an ultra-high-speed Scheimpflug device to record the deformation of the co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405044
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Maintaining mature and viable retinal pigment epithelial cells (RPE) in vitro has proven challenging. Investigating compounds that can promote RPE-viability and maturation is motivated by RPE transplantation research, the quest to understand RPE physiology, and a desire to modulate RPE in pathological states. W...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1809001
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To determine the relative expression of metallothionein isoforms and their differential induction by oxidative stress in cultured RPE cells and to localize the isoforms in the human chorioretinal complex. METHODS:Total RNA was isolated from cultured human retinal pigment epithelial cells using TRI-Reagent. An ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.1.12.5426
更新日期:2002-01-01 00:00:00
abstract::Epsilon is a recently described eye lens protein from Rana temporaria, an anuran amphibian. It is oligomeric with a subunit M.W. of 35K. The cDNA coding for 35K E- in frog lens does not show any homology with cDNA's coding for alpha-, beta-, gamma-, and delta-crystallins. Immunologically, it also does not react with a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609000014
更新日期:1986-10-01 00:00:00
abstract:PURPOSE:To report the presence of hyper-reflective dots in the vitreous cavity using spectral-domain optical coherence tomography (SD-OCT) in patients with acute symptomatic posterior vitreous detachment (PVD) and investigate their association with the presence of retinal tear. METHODS:The medical records of 77 patien...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1289226
更新日期:2017-08-01 00:00:00