Abstract:
PURPOSE:The mechanism by which prostaglandin(PG)F2 alpha increases uveoscleral outflow and lowers intraocular pressure in primates is not known. In cultured human ciliary muscle cells, PGF2 alpha induces the expression of the protooncogene c-fos which is known to induce the transcription of genes such as matrix metalloproteinase-1 (MMP-1) and MMP-3 in other cell systems. As these enzymes are initially secreted as proenzymes, the present study was undertaken to determine if PG treatment induces ciliary muscle cells to secrete either proMMP-1 or proMMP-3. METHODS:Human ciliary smooth muscle cells were grown to confluence in monolayer cell cultures and then treated with PGF2 alpha, 17-phenyltrinor-PGF2 alpha, or 11-deoxy-PGE1. Medium harvested at various times after treatment was assayed for proMMP-1 and proMMP-3 content using sandwich ELISAs. RESULTS:Three days after adding 10 nM PGF2 alpha, proMMP-1 and proMMP-3, concentrations in the culture medium were increased by 254 +/- 33% (mean +/- SE) and 128 +/- 13%, respectively. Compared with vehicle controls, 24 h treatment with 200 nM PGF2 alpha, 17-phenyltrinor-PGF2 alpha, or PGE1, increased proMMP-1 by 116 +/- 29%, 169 +/- 26%, and 273 +/- 16%, respectively. In parallel experiments, proMMP-3 was increased by 99 +/- 18%, 82 +/- 24%, and 214 +/- 16%, respectively. CONCLUSIONS:These results suggest that induction of MMPs in situ following topical PG treatment may degrade ciliary muscle extracellular matrix and possibly contribute to increased uveoscleral outflow, as well.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Lindsey JD,Kashiwagi K,Boyle D,Kashiwagi F,Firestein GS,Weinreb RNdoi
10.3109/02713689609017628subject
Has Abstractpub_date
1996-08-01 00:00:00pages
869-75issue
8eissn
0271-3683issn
1460-2202journal_volume
15pub_type
杂志文章abstract:PURPOSE:To facilitate studies of human retina and utilization of human retinal tissue for treatment of retinal diseases, we studied morphologic preservation in postmortem human retina. METHODS:Morphology of retinas from thirty-one human eyes was examined using light and electron microscopy. The inner and outer retina,...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract:PURPOSE:Basal cell carcinoma (BCC) is a locally invasive skin tumor which can be subdivided into a circumscribed nodular and an invasive fibrosing subtype. There is increasing evidence that macrophages play an important role in interacting between tumor cells and their microenvironment, thereby affecting not only the i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1478980
更新日期:2018-09-01 00:00:00
abstract::In primates, ciliary muscle contraction causes accommodation and facilitates aqueous outflow. In living rhesus monkeys, accommodative, outflow facility, and ciliary muscle movement responses to cholinergic agonists all decline with age. We developed an apparatus to determine in vitro whether the latter is related to i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309024623
更新日期:1993-05-01 00:00:00
abstract:PURPOSE:To evaluate the inhibitory effect of human fetal retinal pigment epithelium (HFRPE) on the activation of human T-cells. METHODS:Pure cultures of HFRPE cells were incubated with purified human T-cells in three different activation assays: 1) allogenic peripheral blood mononuclear cells; 2) OKT3 coated beads in ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.6.430.5269
更新日期:1999-06-01 00:00:00
abstract:PURPOSE:To evaluate serum and aqueous humor levels of fetuin-A in patients with pseudoexfoliation syndrome (PEXS) in comparison with those of age- and sex-matched healthy subjects. MATERIALS AND METHODS:This prospective study included 25 patients with PEXS and 25 control subjects who were undergoing cataract surgery w...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1324629
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:To establish autologous iris pigment epithelial (IPE) cell transplantation in monkey eyes. METHODS:Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cell culture was performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:The present study was conducted to develop and characterize a functional primary culture of pigmented rabbit conjunctival epithelial cells on permeable support exhibiting tight barrier properties. METHODS:Conjunctival epithelial cells were isolated by 0.2% protease treatment, cultured at 0.5-1.8 x 10(6) cells/...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608995151
更新日期:1996-12-01 00:00:00
abstract::Two anti-fibrotic agents were evaluated in vitro for potential application in trabeculectomy. The rate of collagen formation by cultured rabbit corneal fibroblasts was determined by [3H]proline uptake and hydroxylation assay. Incubation of fibroblasts, for 96 hours, with 16 mM 2,4-pyridine dicarboxylic acid (2,4-PDCA)...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309001833
更新日期:1993-06-01 00:00:00
abstract::Thyroxine-treated human lens epithelial cells, HLE B-3, were grown in either a normoxic or hyperoxic atmosphere as a first step in identifying factors related to their increased viability. Reactive oxygen species, ROS, and the apparent mitochondrial membrane potential, Delta Psi(m), were measured using fluorescent pro...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802167554
更新日期:2008-07-01 00:00:00
abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.669005
更新日期:2012-06-01 00:00:00
abstract::EDGF, a growth factor purified from bovine retina is able to increase the rate of wound healing of rabbit corneal epithelium in a dose dependent way. Two applications a day are enough to obtain the maximum rate. The most purified preparation of EDGF is as effective as EGF or pFGF in promoting this phenomenon and allow...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003059
更新日期:1984-04-01 00:00:00
abstract:PURPOSE:So far tear substitutes have demonstrated a limited role in restoring ocular surface damage in dry eye syndrome (DES). The aim of this study was to assess the efficacy of a new ocular surface modulator in an in vitro model of human corneal epithelium (HCE) damaged by severe osmotic stress mirroring the features...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1184282
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:Acanthamoeba keratitis is a sight-threatening corneal infection. It is known that: (i) more amoebae bind to the surface of injured corneas than to the normal corneal surface and (ii) mannose-containing glycoproteins (GPs) possess binding sites for Acanthamoeba. The present study was undertaken to determine whet...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::DNA sequence analysis of overlapping shotgun and restriction fragments have revealed the entire sequence of the human lens fiber cell intrinsic membrane protein MP19 gene (also termed MP17, MP18, and MP20). The 8,056 bp MP19 gene contains 5 exons encoding a mature protein of 173 amino acids, which displayed a very hig...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309033503
更新日期:1993-12-01 00:00:00
abstract::The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS (arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on pol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020325
更新日期:1991-10-01 00:00:00
abstract:PURPOSE:To optimize cultured human corneal endothelial cell (cHCEC) sheet transplantation technique for maintenance of cHCEC viability. MATERIALS AND METHODS:cHCEC sheets cultured on a collagen scaffold were covered with or without Viscoat® and exposed to humidified air in the incubator. cHCEC sheets with or without V...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1101774
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To optimise the high efficiency, non-viral transfer of DNA to retinal pigment epithelial (RPE) cells in vitro. METHODS:A mammalian expression vector (pcDNA3.1) containing a firefly luciferase (luc) cDNA was used to transfect RPE cells using different chemical methods; calcium phosphate, DEAE-dextran and, lipos...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract:PURPOSE:To investigate epithelial cell adhesion and proliferation on a newly developed elastin-like polymer (ELP) that mimics the functional characteristics of extracellular matrices. MATERIALS AND METHODS:A genetically engineered ELP with cell attachment sequences was adsorbed onto glass coverslips as 1, 2, or 3 mole...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802542053
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:To investigate the effects of rapamycin on the TNF-α-induced secretion of interleukin-6 (IL-6) and IL-8 in orbital fibroblasts and its possible mechanism. MATERIALS AND METHODS:Orbital fibroblasts were obtained from patients with thyroid-associated ophthalmopathy. IL-6 and IL-8 levels were measured by enzyme-l...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.750368
更新日期:2013-06-01 00:00:00
abstract:PURPOSE:We report on the use of subconjunctival bevacizumab on corneal vessel density in recurrent pterygia. METHODS:The charts of 5 patients with recurrent pterygium, who received subconjunctival injections of bevacizumab (2.5 mg/0.1 ml) were retrospectively reviewed. Ophthalmic evaluation included Snellen visual acu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701799101
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:Previous studies revealed an association of tumor necrosis factor super family-15 (TNFSF15) gene polymorphisms with several autoimmune diseases, but their roles in the development of Graves' disease (GD) and Graves' ophthalmopathy (GO) remain unclear. The aim of this study was to explore the correlation between...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1705494
更新日期:2020-07-01 00:00:00
abstract::The possible roles of monocytes and lymphocytes in vitreous fibrosis were examined by injecting various numbers of the peripheral cell types into the rabbit vitreous. Our results indicated that the degree of vitreous fibrosis and the presence of traction retinal detachment corresponded to the number of cells injected....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033223
更新日期:1988-12-01 00:00:00
abstract:PURPOSE:To characterize the effect of glutamate receptor activation/inhibition on the secretion of vascular endothelial growth factor (VEGF) in retina-specific glial (Müller) cells under experimental conditions of hyperglycemia and hypoxia, two intrinsic pathologic conditions of diabetic retinopathy. METHODS:Purified ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.483312
更新日期:2010-08-01 00:00:00
abstract::Purpose: Dual wavelength retinal oximetry allows the quantification of oxygen saturation in the larger retinal vessels. However, the technique might be refined further by identifying factors that are responsible for the different oxygen saturations in first order arterioles from the same eye shortly after their branch...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1524015
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) play an important role in the degradation of extracellular matrix (ECM) proteins in the retina. Breakdown of ECM proteins results in neovascularization and tractional retinal detachment, which eventually lead to the symptoms of proliferative diabetic retinopathy. Müller cells ar...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.682635
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:To assess the potential impact of corneal crosslinking treatment (365 nm ultraviolet (UV)A irradiation with riboflavin) on the material properties of Intracorneal Rings Segments (ICRS). MATERIALS AND METHODS:Material properties were studied using FT-IR spectroscopy and UV-Vis spectroscopy. Rings were examined:...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.941071
更新日期:2015-05-01 00:00:00
abstract::Purified Pseudomonas aeruginosa elastase cleaved a 65 kDa gelatinase [inactive proenzyme form of matrix metalloproteinase (MMP-2)] from human corneal fibroblasts into a biologically active fragment with an approximate molecular mass of 58 kDa. However, purified pseudomonal alkaline protease did not cleave MMP-2 apprec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209015082
更新日期:1992-11-01 00:00:00
abstract:AIM:To investigate microscopic changes in corneal morphology in chloroquine (CQ)-treated patients without corneal abnormalities being diagnosable by slit lamp microscopy. METHODS:Thirty-six eyes of 36 patients with rheumatoid arthritis (RA) were divided into two groups: group 1 included 26 patients receiving chloroqui...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.631719
更新日期:2012-04-01 00:00:00
abstract:PURPOSE:Retinal ischemia and neovascularization (NV) are important components of many retinal disorders. To facilitate further investigation of retinal ischemia and neovascularization, we sought to develop a reproducible in vivo experimental model of venous occlusion by photodynamic thrombosis in rats. METHODS:After a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.1.26.5116
更新日期:1997-01-01 00:00:00
abstract::A bovine lens "native" plasma membrane fraction containing its full compliment of extrinsic proteins was prepared by sucrose density centrifugation of the water insoluble fraction. The major membrane fraction was found at the 25/45% sucrose interface. This fraction contained 73% of the total water insoluble phospholip...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209000748
更新日期:1992-08-01 00:00:00