Abstract:
PURPOSE:To optimise the high efficiency, non-viral transfer of DNA to retinal pigment epithelial (RPE) cells in vitro. METHODS:A mammalian expression vector (pcDNA3.1) containing a firefly luciferase (luc) cDNA was used to transfect RPE cells using different chemical methods; calcium phosphate, DEAE-dextran and, liposomes-based transfection techniques. Transfection was optimised for both dose and time of exposure. The efficiency of gene transfer and cytotoxicity was measured 48 hours post-transfection using luciferase and MTT assays, respectively. The percentage of transfected cells (using optimal conditions) was determined with a construct expressing a jellyfish green fluorescent protein (GFP) using flow cytometery. RESULTS:Calcium phosphate and DEAE-dextran techniques failed to transfect the vector and led to high cytotoxicity. Liposomes-based methods successfully transferred the vector to RPE cells, but the efficiency varied for different liposomes; Tfx-50 > Lipofectin > Lipofectamine > Cellfectin > DMRIE-C. No significant cytotoxicity was observed with any of the liposome treatments. Optimal transfection was achieved with Tfx-50 at a 3:1 ratio of DNA:liposome; between 12-15% of cells being transfected. CONCLUSIONS:Efficient and non-toxic transfer of functional genes into primary RPE cells in vitro can be successfuly achieved by liposomes-based techniques. Tfx-50 appears to be a promising non-viral vector for RPE gene transfer.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Abul-Hassan K,Walmsley R,Boulton Msubject
Has Abstractpub_date
2000-05-01 00:00:00pages
361-6issue
5eissn
0271-3683issn
1460-2202journal_volume
20pub_type
杂志文章abstract:BACKGROUND:Interleukin-6 (IL-6) is produced by a variety of cells involved in inflammation and acts as local intensification signals in pathological processes associated with chronic eye inflammation. This meta-analysis was performed to provide a better understanding of the relationship between IL-6 and diabetic retino...
journal_title:Current eye research
pub_type: 杂志文章,meta分析
doi:10.1080/02713683.2019.1570274
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To investigate the effect of topical iganidipine, a new dihydropyridine derivative calcium channel blocker, on impairment of the ocular circulation caused by endothelin-1 (ET-1), we examined modification of the effect of ET-1 on visual-evoked potential (VEP) in albino rabbits. METHODS:To clarify whether VEP co...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract:PURPOSE:The mechanisms involved in the reported beneficial effects of Calcium dobesilate monohydrate (CaD) for the treatment of diabetic retinopathy (DR) remain to be elucidated. The main aim of the present study is to examine whether CaD prevents early events in the pathogenesis of DR such as neurodegeneration and vas...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1302591
更新日期:2017-09-01 00:00:00
abstract::Reduced glutathione (0.3 mM) stimulates the activity of sodium-potassium activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione, however, has no effect on Na+K+ATPase activity in the same tissue, although it does inhibit magnesium activated ATPase...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019985
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:To assess the upper eyelid kinematics during spontaneous blinking in unilateral paralytic lagophthalmos before and after upper eyelid load with gold weight (GW) or autogenous temporalis fascia (TF) sling. DESIGN:Comparative case series. SUBJECTS:Patients with long-standing unresolved unilateral facial palsy w...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.3109/02713683.2015.1031252
更新日期:2016-04-01 00:00:00
abstract::Purpose: To map the time course of changes in intraocular straylight of the human eye 1 year after initial lens wearing for orthokeratology treatment using the objective double-pass technique. Materials and Methods: A total of 35 subjects (19 males and 16 females) completed the study. The mean age was 11.46 ± 2.33 yea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514056
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:The aim of this review is to evaluate the efficacy of available Purtscher's Retinopathy treatments. MATERIALS AND METHODS:In order to collect single-case reports, electronic searches were conducted in several databases including PubMed, Embase, Web of Science, China National Knowledge Infrastructure, SinoMed...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.1080/02713683.2016.1255335
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:The aim of this pilot study was to determine the presence of complement activation products in tears from pre- and postkeratoplasty eyes and the fellow eyes in order to investigate the activation of the classical and alternative pathways of the complement system in the early postkeratoplasty period. METHODS:Te...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.24.2.99.8165
更新日期:2002-02-01 00:00:00
abstract::DNA sequence analysis of overlapping shotgun and restriction fragments have revealed the entire sequence of the human lens fiber cell intrinsic membrane protein MP19 gene (also termed MP17, MP18, and MP20). The 8,056 bp MP19 gene contains 5 exons encoding a mature protein of 173 amino acids, which displayed a very hig...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309033503
更新日期:1993-12-01 00:00:00
abstract::Perturbations in capillary endothelial cell function are early events in the pathogenesis of diabetic retinopathy. We used tritiated thymidine autoradiography to study the turn-over rate of retinal capillary endothelial cells in retinovascular preparations from normal, untreated diabetic and diabetic mice treated with...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909025802
更新日期:1989-07-01 00:00:00
abstract::Two alternative methods for the study of the lens cytoskeleton are described which serve to overcome some of the difficulties imparted by the unique biology of the lens. The first technique involves rapid freezing, thick sectioning, and selective extraction and/or fixation of the lens section. This approach offers sev...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008997582
更新日期:1990-11-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) are involved in posterior capsule opacification (PCO), but the mechanisms that promote MMP expression are yet to be determined. In this study, we investigated whether type I collagen, which is only detected in aged or cataractous lens capsules, affects the expression and activat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.853194
更新日期:2014-05-01 00:00:00
abstract:PURPOSE:To assess the distribution of dark-light changes in iris features and associated factors amongst American Caucasians, American Chinese and mainland Chinese aged 40 years and over. METHODS:Four gender- and age-matched cohorts: Caucasians, American Chinese, Southern Chinese and Northern Chinese comprised the stu...
journal_title:Current eye research
pub_type: 杂志文章,多中心研究
doi:10.3109/02713683.2012.660593
更新日期:2012-07-01 00:00:00
abstract:PURPOSE:Age-related macular degeneration (AMD) is the most common cause of visual loss. The dry AMD is characterized by retinal pigment epithelium (RPE) death and changes in AMD lead to severe loss of vision. Coumarin-derived esculetin has a number of therapeutic and pharmacological effects such as anti-inflammatory an...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1481517
更新日期:2018-09-01 00:00:00
abstract::Blood and lymphatic vessel proliferation into the cornea occurs after severe trauma. Whilst the events early in blood vessel growth are reasonably understood, little is known of the early events in lymphangiogenesis. In this study, a model for proliferating lymphatic vessels using tritiated-thymidine autoradiography a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013898
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To compare the quality of corneal endothelium of precut Descemet-stripping automated endothelial keratoplasty (DSAEK) tissue when transported with and without the anterior lamellar corneal tissue (ALCT) when organ-culture corneal storage methods are used. METHODS:After microkeratome-assisted excision of anteri...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.648045
更新日期:2012-02-01 00:00:00
abstract::Antifibrosis agents have improved the success of glaucoma filtration surgery, although undesired side effects are not readily reversible and may present a major limitation in the use of these agents. Our purpose was to study the efficacy of cytosine arabinoside (Ara-C) as an adjunctive antimetabolite in glaucoma surge...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689409025140
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:In tissues outside the brain, vascular endothelial growth factor-A (VEGF) causes vascular hyper-permeability by opening of inter-endothelial junctions and induction of fenestrations and vesiculo-vacuolar organelles (VVOs). In preliminary studies, we observed that in blood-retinal barrier endothelium, other cell...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:The aim of this study was to characterize a standardized porcine ex-vivo testing system for intraocular pressure (IOP) monitoring after vitrectomy with different endotamponades. METHODS:Twenty-four pig eyes, six per endotamponade group were obtained immediately postmortem. After pars plana vitrectomy, vitreous...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1297461
更新日期:2017-08-01 00:00:00
abstract::Levels of rhodopsin in the photoreceptors of Long-Evans rats were reduced by approximately 55% through dietary deprivation of vitamin A. The interaction of visual pigment with G-protein was examined in receptor outer segment (ROS) membranes obtained from these animals. A binding assay was used to quantitate affinity o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908996390
更新日期:1989-04-01 00:00:00
abstract::Purpose: The purpose of this study is to present the study design and one month's preliminary results of a 2-year randomized trial, Combined Atropine with Orthokeratology (AOK), for myopia control study. Methods: Children aged 6 to <11 years and with 1.00-4.00 D myopia were randomly assigned to AOK group or ortho-k al...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2019.1568501
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:Experiments were performed to evaluate the effect of various pro- and anti-inflammatory cytokines on the human fetal retinal pigment epithelium's (HFRPE) expression of major histocompatibility complex (MHC) and costimulatory molecules. METHODS:Pure cultures of HFRPE cells were isolated. HFRPE cells were incuba...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.4.285.5456
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:Phoria is used in the diagnosis of binocular dysfunctions, such as convergence insufficiency. A common treatment for convergence insufficiency is vision therapy, yet it is controversial whether the phoria changes as a result of rehabilitation. Phoria can vary due to the duration and/or the type of prior visual ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.694551
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a member of the TIMP family of proteins, playing a significant role in the control of extracellular matrix remodelling. TIMP-3 might play a role in the regulation of retinal neovascularization during progression of diabetic retinopathy. Recently, three novel p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.91.15954
更新日期:2003-08-01 00:00:00
abstract:PURPOSE:Dry eye is a common condition known to have many systemic co-morbidities. We aim to report the frequency of depression and anxiety suspects in eye clinic patients and explore the association between dry eye signs and symptoms with depression and anxiety. MATERIALS AND METHODS:Adult outpatients were recruited f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1056804
更新日期:2016-05-01 00:00:00
abstract:PURPOSE:To investigate the therapeutic role of topical and oral fluconazole treatment using a rabbit model of Aspergillus fumigatus keratitis. METHODS:Aspergillus fumigatus spores were injected into the corneal stroma of the right eye of 20 rabbits. Forty-eight hours later the rabbits were randomly divided into three ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.2.113.14514
更新日期:2003-02-01 00:00:00
abstract::5-Fluorouracil (5-FU) and Mitomycin C (MMC) are used as adjunct chemotherapy during glaucoma filtering surgery to suppress conjunctival fibroblast proliferation. Since part of these agents may gain access to the anterior chamber and cause cytotoxicity to the corneal endothelium we set up an in vitro system to establis...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209001812
更新日期:1992-06-01 00:00:00
abstract:PURPOSE:To evaluate the anatomical correlation between fellow eyes for bilateral second-line anti-VEGF treatment in eyes with bilateral diabetic macular edema (DME) with incomplete response to first-line bevacizumab therapy. METHODS:Seventy-four eyes (n = 37 patients) with bilateral-DME having incomplete response to f...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1812085
更新日期:2020-08-30 00:00:00
abstract:PURPOSE:The present study was conducted to develop and characterize a functional primary culture of pigmented rabbit conjunctival epithelial cells on permeable support exhibiting tight barrier properties. METHODS:Conjunctival epithelial cells were isolated by 0.2% protease treatment, cultured at 0.5-1.8 x 10(6) cells/...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689608995151
更新日期:1996-12-01 00:00:00
abstract::The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS (arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on pol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020325
更新日期:1991-10-01 00:00:00