Abstract:
PURPOSE:To evaluate the inhibitory effect of human fetal retinal pigment epithelium (HFRPE) on the activation of human T-cells. METHODS:Pure cultures of HFRPE cells were incubated with purified human T-cells in three different activation assays: 1) allogenic peripheral blood mononuclear cells; 2) OKT3 coated beads in the presence of accessory cells; and 3) stimulation with phorbol ester and phytohemagglutinin. RESULTS:HFRPE cells suppressed the activation of T-cells in all three assays. The mechanism of HFRPE mediated T-cell suppression was apoptosis. The role of Fas ligand(FasL)/Fas-mediated T-cell suppression was excluded, since FasL protein or mRNA could not be detected on HFRPE cells with flow cytometry and by reverse transcriptase polymerase chain reaction, respectively. Additionally, the inhibitory effect of HFRPE cells could not be blocked by anti-Fas ligand or antagonistic anti-Fas antibodies. Moreover, HFRPE cells suppressed the proliferation of anti-CD3 mAb mediated T-cell proliferation of murine splenocytes isolated from lpr mice. The inhibitory effect of HFRPE cells was not PGE2 mediated, since indomethacin could not restore the T-cell activation. Although the HFRPE mediated T-cell apoptosis was cell-cell contact independent, it was not induced by secretion of TNF-alpha, TGF-beta, or IL-10. The ratio between HFRPE and T-cells had a major impact on the HFRPE's inhibitory effect. CONCLUSIONS:HFRPE cells suppressed the activation of human T-cells by induction of T-cell apoptosis through a process that involves the secretion of soluble factors. The HFRPE mediated T-cell suppression was dependent on the ratio between HFRPE and T-cells. This undefined pathway of T-cell apoptosis may play a role in the maintenance of immune privilege in the subretinal space and may reduce the severity of the immune response after HFRPE transplantation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Rezai KA,Semnani RT,Farrokh-Siar L,Hamann KJ,Patel SC,Ernest JT,van Seventer GAdoi
10.1076/ceyr.18.6.430.5269subject
Has Abstractpub_date
1999-06-01 00:00:00pages
430-9issue
6eissn
0271-3683issn
1460-2202journal_volume
18pub_type
杂志文章abstract::The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS (arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on pol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020325
更新日期:1991-10-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.548892
更新日期:2011-04-01 00:00:00
abstract::Many tissues which require vitamin A store the vitamin as long-chain fatty acyl esters of retinol. As part of a study designed to characterize vitamin A metabolism in the lacrimal gland, which transports retinol from blood to lacrimal gland fluid, extracts from lacrimal glands of rabbits and rats were analyzed by non-...
journal_title:Current eye research
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doi:10.3109/02713688809015147
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abstract:PURPOSE:We investigated the impact of target properties on vertical fusion amplitude (VFA) using a 3D display platform; the performance of the subjects allowed us to assess how central and peripheral retina regions interact during the fusion process. MATERIAL AND METHODS:Fourteen subjects were involved in the test. VF...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1196704
更新日期:2017-03-01 00:00:00
abstract::A reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H)-dependent hydrogen peroxide-generating activity of rat ocular tissue was investigated cytochemically utilizing the cerium method. NADPH or NADH oxidation resulted in electron-dense fine granular deposits on the corneal epithelial and endothelial plasma me...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688808997249
更新日期:1988-09-01 00:00:00
abstract:PURPOSE:To compare the retinal sensitivity, using Microperimeter and the morphological changes in retinal layers, using Spectral Domain Optical Coherence Tomography (SD-OCT), between myopes and age-matched emmetropes. METHODS:Twenty-nine myopic eyes and 29 emmetropic eyes were enrolled in this prospective case control...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.599105
更新日期:2011-10-01 00:00:00
abstract::The pigmented epithelium of the vertebrate retina phagocytizes the discarded tips of photoreceptors and it is likely that a specific cellular recognition process is involved in this phenomenon. The apical surface of retinal pigmented epithelium (RPE) contains microvilli which interdigitate with the outer segment regio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034868
更新日期:1987-08-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.5.309.13494
更新日期:2002-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
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journal_title:Current eye research
pub_type: 临床试验,杂志文章
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更新日期:2013-08-01 00:00:00
abstract::Trabecular meshwork cells from an inbred strain of mice (A/J) were established in tissue culture. Within 1 hour of enucleation, tissue containing the cornea and the chamber angle was excised and placed in tissue culture. Two to five days later, three cell types grew from the explants. Two of these cell types, corneal ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020340
更新日期:1991-11-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688908996390
更新日期:1989-04-01 00:00:00
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journal_title:Current eye research
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更新日期:2019-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600557030
更新日期:2006-03-01 00:00:00
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journal_title:Current eye research
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更新日期:2018-12-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713680902850075
更新日期:2009-05-01 00:00:00
abstract::Processing of visual information within the retina depends in large measure upon a complement of chemical neurotransmitters which are released at synaptic contacts between individual neurons. Numerous investigators have participated in the identification of many of these transmitters and their assignment to specific n...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713689609003465
更新日期:1996-07-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409167214
更新日期:1984-07-01 00:00:00
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journal_title:Current eye research
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更新日期:2018-06-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017650
更新日期:1996-10-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.648045
更新日期:2012-02-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688408997233
更新日期:1984-03-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章,meta分析
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更新日期:2019-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609000770
更新日期:1996-05-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688609020055
更新日期:1986-04-01 00:00:00
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journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00