Multiple selenocysteine content of selenoprotein P in rats.

Abstract:

:Partially purified selenoprotein P from rat plasma was digested with either trypsin, endoprotease Lys-C, or endoprotease Arg-C and analyzed by high pressure liquid chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Several 75Se-labeled peptides were detected. The moles of selenium in selenoprotein P were estimated based on the 75Se content of the 75Se-labeled peptide fragments. Using this method, selenoprotein P was shown to contain approximately 9 moles of selenium. This is the first report of a selenoprotein containing more than one selenium per polypeptide. These findings support the proposed function of this protein in selenium transport.

journal_name

J Inorg Biochem

authors

Motchnik PA,Tappel AL

doi

10.1016/0162-0134(90)80060-b

subject

Has Abstract

pub_date

1990-11-01 00:00:00

pages

265-9

issue

3

eissn

0162-0134

issn

1873-3344

pii

0162-0134(90)80060-B

journal_volume

40

pub_type

杂志文章
  • Cytochrome c oxidase, ligands and electrons.

    abstract::We present hereby an overview of the reactions of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, with ligands (primarily oxygen) and electrons, pointing out where necessary unresolved facts or questionable interpretations. ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.jinorgbio.2004.10.011

    authors: Brunori M,Giuffrè A,Sarti P

    更新日期:2005-01-01 00:00:00

  • A singular value decomposition approach for kinetic analysis of reactions of HNO with myoglobin.

    abstract::The reactions of several horse heart myoglobin species with nitrosyl hydride, HNO, derived from Angeli's salt (AS) and Piloty's acid (PA) have been followed by UV-visible, (1)H NMR and EPR spectroscopies. Spectral analysis of myoglobin-derived speciation during the reactions was obtained by using singular value decomp...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2012.10.005

    authors: Zapata AL,Kumar MR,Pervitsky D,Farmer PJ

    更新日期:2013-01-01 00:00:00

  • The formation and nature of the mixed valence copper-D-penicillamine-chloride cluster in aqueous solution and its relevance to the treatment of Wilson's disease.

    abstract::Complex formation between D-penicillamine (Pen) and copper(II) ions has been studied under simulated physiological conditions in both the presence and absence of the blood plasma constituents albumin, alanine, histidine, and zinc(II). Chromatographic and uv/vis and electron spin resonance (esr) spectroscopic methods w...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(00)80020-3

    authors: Laurie SH,Prime DM

    更新日期:1979-11-01 00:00:00

  • Origins of aging mass loss in recombinant N-terminus and C-terminus deletion mutants of the heme-PAS biosensor domain BjFixLH(140-270).

    abstract::Nine recombinant FixL heme domains from Bradyrhizobium japonicum previously were shown to exhibit mass instability independent of many environmental factors (J.D. Satterlee, C. Suquet, A. Bidwai, J. Erman, L. Schwall, R. Jimenez, Biochemistry 47 (2008) 1540-1553). Two of those recombinant proteins were produced in rem...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2011.01.015

    authors: Satterlee JD

    更新日期:2011-05-01 00:00:00

  • The interesting DNA-binding properties of three novel dinuclear Ru(II) complexes with varied lengths of flexible bridges.

    abstract::Three binuclear Ru(II) complexes with two [Ru(bpy)(2)(pip)](2+)-based subunits {where bpy=2,2'-bipyridine and pip=2-phenylimidazo[4,5-f][1,10]phenanthroline} being linked by varied lengths of flexible bridges, were synthesized and characterized by (1)H NMR, elemental analysis, UV-visible (UV-vis) and photoluminescence...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2010.12.004

    authors: Ju CC,Zhang AG,Yuan CL,Zhao XL,Wang KZ

    更新日期:2011-03-01 00:00:00

  • The role of protein binding of trivalent arsenicals in arsenic carcinogenesis and toxicity.

    abstract::Three of the most plausible biological theories of arsenic carcinogenesis are protein binding, oxidative stress and altered DNA methylation. This review presents the role of trivalent arsenicals binding to proteins in arsenic carcinogenesis. Using vacuum filtration based receptor dissociation binding techniques, the l...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.jinorgbio.2007.10.021

    authors: Kitchin KT,Wallace K

    更新日期:2008-03-01 00:00:00

  • Effective cleavage of phosphodiester promoted by the zinc(II) and copper(II) inclusion complexes of β-cyclodextrin.

    abstract::To construct the model of metallohydrolase, two inclusion complexes [MLCl2(β-CD)] (1, M=Zn(II); 2, M=Cu(II); L=N,N'-bis(2-pyridylmethyl)amantadine; β-CD=β-cyclodextrin) were synthesized by mixing β-CDs with the pre-synthesized complexes G1, [ZnLCl2] and G2, [CuLCl2]. Structures of G1, G2, 1 and 2 were characterized by...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2016.07.011

    authors: Zhou YH,Chen LQ,Tao J,Shen JL,Gong DY,Yun RR,Cheng Y

    更新日期:2016-10-01 00:00:00

  • Active site structure of SoxB-type cytochrome bo3 oxidase from thermophilic Bacillus.

    abstract::Two-subunit SoxB-type cytochrome c oxidase in Bacillus stearothermophilus was over-produced, purified, and examined for its active site structures by electron paramagnetic resonance (EPR) and resonance Raman (RR) spectroscopies. This is cytochrome bo3 oxidase containing heme B at the low-spin heme site and heme O at t...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(00)00145-8

    authors: Uchida T,Tsubaki M,Kurokawa T,Hori H,Sakamoto J,Kitagawa T,Sone N

    更新日期:2000-11-01 00:00:00

  • Effect of peripheral trifluoromethyl groups in artificial iron porphycene cofactor on ligand binding properties of myoglobin.

    abstract::An iron porphycene, a structural isomer of iron porphyrin, with trifluoromethyl groups at the peripheral position of the framework was incorporated into sperm whale apomyoglobin. The prepared myoglobin shows the higher O(2) affinity than the native protein. However, the oxygen affinity of the reconstituted myoglobin i...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2007.07.032

    authors: Matsuo T,Ito K,Nakashima Y,Hisaeda Y,Hayashi T

    更新日期:2008-02-01 00:00:00

  • Mechanistic aspects of the interaction between selenium and arsenic.

    abstract::Selenium is an essential trace element for humans and other animals, and there is mounting evidence for the efficacy of certain forms of selenium as cancer-chemopreventive compounds. However, over the years, numerous elements such as As, Cu, Zn, Cd, Hg, Sn, Pb, Ni, Co, Sb, Bi, Ag, Au, and Mo have been found to inhibit...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章,评审

    doi:10.1016/j.jinorgbio.2005.03.006

    authors: Zeng H,Uthus EO,Combs GF Jr

    更新日期:2005-06-01 00:00:00

  • Structural effects of Zn(2+) on cell membranes and molecular models.

    abstract::Zinc is an essential element for nutrition as well as for the proper development and function of brain cells, and its traces are present in a wide range of foods. It is a constituent of many enzyme systems and is an integral part of insulin and of the active site of intracellular enzymes. However, excessive accumulati...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2009.02.009

    authors: Suwalsky M,Novoa V,Villena F,Sotomayor CP,Aguilar LF,Ronowska A,Szutowicz A

    更新日期:2009-05-01 00:00:00

  • Luminescent pH sensing and DNA binding properties of a novel ruthenium(II) complex.

    abstract::A new Ru(II) complex, [Ru(bpy)(2)(dhipH3)](ClO4)(2) (in which bpy=2,2'-bipyridine, dhipH(3)=3,4-dihydroxy-imidado[4,5-f][1,10]-phenanthroline), was synthesized and characterized, and the pH effect on the emission spectra of the complex was studied. The interaction of the complex with calf thymus DNA was investigated b...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2004.02.021

    authors: Bai GY,Wang KZ,Duan ZM,Gao LH

    更新日期:2004-06-01 00:00:00

  • The effect of pyrophosphate, tripolyphosphate and ATP on the rate of the Fenton reaction.

    abstract::It has been recently reported that pyrophosphate, tri-polyphosphate, ATP and analogous ligands considerably decrease the yield of hydroxyl radicals by the Fenton reaction under conditions where [H(2)O(2)]>[Fe(II)L(n)]. It was suggested that this effect is due to the slowing down of the Fenton reaction by these ligands...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2011.01.009

    authors: Rachmilovich-Calis S,Masarwa A,Meyerstein N,Meyerstein D

    更新日期:2011-05-01 00:00:00

  • Influence of the introduction of a triphenylphosphine group on the anticancer activity of a copper complex.

    abstract::Aiming at obtaining new copper complexes with good cytotoxicity against cancer cells, triphenylphosphine (TPP) was introduced to obtain insight into the influence of the co-ligands. In this paper, two copper complexes, Cu(2-pbmq)(CH3OH)Br2 (1) and [Cu(2-pbmq)(TPP)Br]2 (2) were designed, synthesized, and characterized ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2020.111102

    authors: Li S,Zhao J,Guo Y,Mei Y,Yuan B,Gan N,Zhang J,Hu J,Hou H

    更新日期:2020-09-01 00:00:00

  • Transition metal complexes of diclofenac with potentially interesting anti-inflammatory activity.

    abstract::An overview is given of the results of metal ion-diclofenac interactions. Several complexes have been synthesized at the University of Ioannina. Binuclear complexes, [Cu(L)2(H2O)]2 x 2H2O and [CuL2(S)]2 where S is H2O, EtOH, DMSO, (CH3)2CO and DMF, and mononuclear complexes, [MnL2(H2O)], [FeL2(H2O)2], [CoL2(H2O)2] x 0...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(99)00175-0

    authors: Kovala-Demertzi D

    更新日期:2000-04-01 00:00:00

  • Prognostic significance of low serum levels of Clara cell phospholipid-binding protein in occupational aluminium neurotoxicity.

    abstract::The relationship between respiratory and neurological effects of exposure to aluminium (Al) was investigated in a group of foundry workers exposed to Al at concentrations below the threshold limit value (TLV) binding in Poland (2.0 mg Al2O3 m(-3)). Neurological and neurophysiological parameters indicated subclinical e...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2005.06.027

    authors: Halatek T,Sinczuk-Walczak H,Rydzynski K

    更新日期:2005-09-01 00:00:00

  • Geometric effects on OO bond scission of copper(II)-alkylperoxide complexes.

    abstract::Copper(II) complexes supported by N3-tridentate ligands, consisting of a rigid cyclic diamine (8-membered cyclic-diamine; L8 or 7-membered cyclic-diamine; L7) and a 2-(2-pyridyl)ethyl (-CH2CH2Py) group, were synthesized and structurally characterized. Reaction of the copper(II) complexes and cumene hydroperoxide (CmOO...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2017.08.016

    authors: Abe T,Morimoto Y,Mieda K,Sugimoto H,Fujieda N,Ogura T,Itoh S

    更新日期:2017-12-01 00:00:00

  • Metal specificity in DNA damage prevention by sulfur antioxidants.

    abstract::Metals such as CuI and FeII generate hydroxyl radical (.OH) by reducing endogenous hydrogen peroxide (H2O2). Because antioxidants can ameliorate metal-mediated oxidative damage, we have quantified the ability of glutathione, a primary intracellular antioxidant, and other biological sulfur-containing compounds to inhib...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2008.06.010

    authors: Battin EE,Brumaghim JL

    更新日期:2008-12-01 00:00:00

  • The syntheses of amphiphilic antimony(V)-phthalocyanines and spectral investigation on their aggregation behaviors in aqueous and non-aqueous solutions.

    abstract::Three amphiphilic antimony(V)-phthalocyanines have been synthesized by treating [Sb(R(4)Pc)(OH)(2)](+) salts in concentrated H(2)SO(4) and isolated as zwitter ions, [Sb(R(4)Pc)(SO(4)H)(SO(4))], where R(4)Pc denotes tetra-substituted phthalocyaninate; R(4)Pc=pc (R=H), tbpc (R=(t)Bu), and tObpc (R=O(n)Bu). Their solubil...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2012.02.021

    authors: Isago H,Kagaya Y,Oyama Y,Fujita H,Sugimori T

    更新日期:2012-06-01 00:00:00

  • Cis- and trans-conformations for peroxynitrite anions.

    abstract::The biochemically important anion, peroxynitrite, is commonly thought to have a cis-conformation which is stable. This is thought to have a cyclic structure, which blocks the formation of the trans-conformation. Furthermore, it is often suggested that the latter structure isomerises to the far more stable nitrate ion....

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(00)00057-x

    authors: Symons MC

    更新日期:2000-03-01 00:00:00

  • The copper(II) binding properties of the cyclic peptide c(HGHK).

    abstract::The new cyclic tetrapeptide c(HGHK) was synthesised in the solid phase and its complexes with copper(II) were studied in aqueous solution at various pH values by means of potentiometric and spectroscopic methods (UV, EPR, CD). Six mononuclear coordination species were clearly identified within the pH range 3-11. Spect...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2004.09.007

    authors: Brasun J,Gabbiani C,Ginanneschi M,Messori L,Orfei M,Swiatek-Kozlowska J

    更新日期:2004-12-01 00:00:00

  • Design, synthesis, and evaluation of phosphorescent Ir(III) complexes with anticancer activity.

    abstract::A range of phosphorescent Ir(III) complexes containing four diverse P^P-chelating ligands of the type [Ir(ppy)2(L)][PF6], (ppy = 2‑phenylpyridine) where L is 1,2‑bis(diphenylphosphino)benzene (L1), 1,2‑bis(diphenylphosphino)ethane (L2), 1,2‑bis(diphenylphosphino)propane(L3) and 1,8‑bis(diphenylphosphino)naphthalene (L...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2019.110703

    authors: Liu Z,Li J,Ge X,Zhang S,Xu Z,Gao W

    更新日期:2019-08-01 00:00:00

  • Modulation of relaxation activity of human topoisomerases by Pt(II)-based complexes.

    abstract::The clinical efficiency of Pt(II)-based drugs is founded on articulate mechanisms of action. Indeed it depends on a balanced combination of metal ion reactivity towards proteins and nucleic acids. Here we analysed the effect of two trans-platinum planar amines in comparison to cisplatin and transplatin on the DNA proc...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2020.111178

    authors: Pinato O,Benettazzo A,Dalla Via L,Farrell NP,Sissi C

    更新日期:2020-10-01 00:00:00

  • cis-1,4-diaminocyclohexane-Pt(II) and -(IV) adducts with DNA bases and nucleosides.

    abstract::A series of new platinum(II) and platinum(IV) adducts of type [P(II)(cis-1,4-DACH)LCl]NO(3,) where cis-1,4-DACH=cis-1,4-diaminocyclohexane, and L=9-ethylguanine, 1-methylcytosine, adenine, adenosine, cytosine, cytidine, guanine, and [Pt(IV)(cis-1,4-DACH)Ltrans-(X)(2)Cl]NO(3), (where Y=hydroxo or acetato), were synthes...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(03)00262-9

    authors: Ali MS,Khokhar AR

    更新日期:2003-09-01 00:00:00

  • In vivo coordination structural changes of a potent insulin-mimetic agent, bis(picolinato)oxovanadium(IV), studied by electron spin-echo envelope modulation spectroscopy.

    abstract::Bis(picolinato)oxovanadium(IV) [VO(pic)2] is one of the most potent insulin-mimetic vanadium complexes. To probe coordination structural changes of this complex in vivo and provide insights into the origin of its high potency, an electron spin-echo envelope modulation (ESEEM) study was performed on organs (kidney, liv...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(99)00204-4

    authors: Fukui K,Fujisawa Y,OhyaNishiguchi H,Kamada H,Sakurai H

    更新日期:1999-11-01 00:00:00

  • A 1H nmr study of the interaction of aurothiomalate ("Myocrisin") with human red blood cells in vitro.

    abstract::The results of 1H spin-echo Fourier transform (SEFT) nuclear magnetic resonance (nmr) experiments suggest that some aurothiomalate binds intracellular glutathione (GSH) when added to suspensions of red cells in vitro. When added to red cell lysates, a specific binding of gold to cysteine of GSH is observed together wi...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(83)85027-2

    authors: Otiko G,Razi MT,Sadler PJ,Isab AA,Rabenstein DL

    更新日期:1983-11-01 00:00:00

  • Selenium-containing proteins of rat kidney.

    abstract::Rat kidney selenium (Se)-containing proteins were studied by isotopic labeling with [75Se]selenite or [75Se]selenomethionine via three routes: oral, intraperitoneal injection, and incubation of kidney slices with the isotope. The two major Se-containing proteins in kidney were fractionated and partially characterized....

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(88)80005-9

    authors: Viljoen AJ,Tappel AL

    更新日期:1988-08-01 00:00:00

  • Cyclic tetranuclear half-sandwich ruthenium(II) complexes with 4,7-phenanthroline and hydroxo bridges: crystal structure, solution behaviour and binding to nucleosides.

    abstract::The reaction between [(eta(6)-p-cymene)Ru(H2O)3]X2 and 4,7-phenanthroline (phen) leads to the formation of the rectangular tetranuclear complexes [(eta(6)-p-cymene)4Ru4(mu-4,7-phen-N4,N7)(2)(mu-OH)4]X4 (X=NO3, 1a; SO3CF3, 1b) which have been structurally characterised by X-ray crystallography. 1H NMR spectroscopic stu...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2007.11.009

    authors: Galindo MA,Quirós M,Romero MA,Navarro JA

    更新日期:2008-05-01 00:00:00

  • Drug-metal interactions: copper(II) complex of the antidiabetic drug acetohexamide and pyridine.

    abstract::The preparation, spectral properties, and crystal structure of a copper(II) complex of 4-acetyl-N-[(cyclohexylamino)carbonyl]benzenesulfonamide, which is known as acetohexamide, and pyridine are reported. The complex Cu(AH)2(py)2, where AH = acetohexamide and py = pyridine, was prepared and characterized by X-ray and ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(90)84012-e

    authors: Pezeshk A,Pezeshk V

    更新日期:1990-03-01 00:00:00

  • Preparation, characterization, and antitumor activity of new ethylenediamine platinum(IV) complexes containing mixed carboxylate ligands.

    abstract::A series of ethylenediamine platinum(IV) complexes of the type PtIV(en)XA2 and PtIV(en)X'2A2, where X = 1,1-cyclobutanedicarboxylato or malonato, X' = chloro, cyclobutanecarboxylato, cyclopentanecarboxylato, or cyclohexanecarboxylato, and A = acetato or trifluoroacetato were synthesized and characterized by elemental ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(93)80015-2

    authors: Khokhar AR,Deng Y,Kido Y,Siddik ZH

    更新日期:1993-05-01 00:00:00