Abstract:
UNLABELLED:OBJECTDIVES: Mouse double minute 2 is a key negative regulator of the p53 protein, a central node in the mediation of tumor suppression. The MDM2 gene contains 2 differently regulated promoters, MDM2-P1 and MDM2-P2, which differ strongly in their biological and clinical importance. METHODS:We assess the clinical significance of the expression of messenger RNA (mRNA) transcripts originating from both MDM2 promoters, measured with quantitative reverse transcription polymerase chain reaction in microdissected tissues from 57 patients with pancreatic ductal adenocarcinoma (PDAC). Furthermore, we determine the clinical relevance of p53 mRNA transcript expression and incorporate the somatic p53 mutational status into our analyses. RESULTS:Interestingly, elevated transcript levels from the P1 promoter, but not the P2 promoter, associate significantly with up to 6.3-fold increased relative risk for tumor-related death (Cox multivariate analysis: P = 0.013). Furthermore, transcripts originating from both MDM2 promoters are found to correlate significantly with p53 mRNA levels (up to r = 0.315; P = 0.017). In addition, low p53 mRNA expression associates with worse PDAC prognosis (relative risk = 2.28; P = 0.021). CONCLUSIONS:This study presents the first differentiated analysis of the MDM2-P1, MDM2-P2, and p53 transcript expression in human PDAC and demonstrates the significant clinical implications of those transcripts. Furthermore, it suggests an additional facet in the regulation of MDM2 via its P1 promoter in this malignancy.
journal_name
Pancreasjournal_title
Pancreasauthors
Grochola LF,Taubert H,Greither T,Bhanot U,Udelnow A,Würl Pdoi
10.1097/mpa.0b013e3181f95104subject
Has Abstractpub_date
2011-03-01 00:00:00pages
265-70issue
2eissn
0885-3177issn
1536-4828journal_volume
40pub_type
杂志文章相关文献
PANCREAS文献大全abstract::Human pure pancreatic juice (PPJ) and serum were analyzed for free fatty acids (FFAs) to study whether the damage to pancreatic cell membranes in pancreatitis is reflected as abnormal FFAs concentration and composition. Patients consisted of 13 normal controls, 7 patients with acute pancreatitis (AP) in remission, and...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198804000-00017
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVES:Serum pancreastatin is a sensitive and specific diagnostic biomarker in neuroendocrine tumors (NETs). Elevated pancreastatin levels are associated with worse progression-free survival and overall survival in small bowel and pancreatic NETs. In this study, we investigated the clinical significance of elevated...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000572
更新日期:2016-08-01 00:00:00
abstract::Changes in pancreatic growth and in mRNA concentrations in rat pancreas were monitored by dot-blot hybridization with cloned cDNAs of rat amylase, chymotrypsinogen B, proinsulin I, and actin during the pre- and postnatal period in the rat. Wistar rats were killed at the 18th day of gestation and at the 1st, 10th, 20th...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199007000-00008
更新日期:1990-07-01 00:00:00
abstract:OBJECTIVE:Multiple organ failure because of systemic inflammatory response in the early phase and sepsis in the late phase is the main contributor to high mortality in severe acute pancreatitis (SAP). High-mobility group box chromosomal protein 1 (HMGB1) was recently identified as a potent proinflammatory mediator and ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000236741.15477.8b
更新日期:2006-11-01 00:00:00
abstract::We investigated the role of platelet-activating factor (PAF) as a priming signal for cytokine-induced neutrophil chemoattractant (CINC) expression by bronchoalveolar macrophages in acute pancreatitis. Pancreatitis was induced by four intramuscular injections of cerulein (50 micrograms/kg at 1-h intervals) in Wistar ra...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199905000-00005
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to clarify the clinical and pathophysiological characteristics of autoimmune pancreatitis (AIP) and its subtypes (lymphoplasmacytic sclerosing pancreatitis [LPSP] and idiopathic duct-centric pancreatitis [IDCP]) seen around the world. METHODS:An international multicenter surve...
journal_title:Pancreas
pub_type: 杂志文章,多中心研究
doi:10.1097/MPA.0b013e3182258a15
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:We examined pancreatic cancer risk in association with hormonal and reproductive factors in a prospective cohort study of 89,835 Canadian women, aged 40-59 at recruitment, who were enrolled in the National Breast Screening Study (NBSS). METHODS:Linkages to national cancer and mortality databases yielded dat...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000160301.59319.ba
更新日期:2005-05-01 00:00:00
abstract::Solitary fibrous tumor (SFT) arising in the pancreas is exceedingly rare, with only 11 cases reported in the English literature. All cases described thus far have exhibited benign histology. We report the first case of malignant SFT of the pancreas. The patient was a 52-year-old woman who presented with obstructive ja...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000350
更新日期:2015-08-01 00:00:00
abstract::Pseudocysts and post-necrotic collections of the pancreas are sometimes treated by percutaneous drainage. In cases of post-necrotic collection, intractable pancreatic juice fistula is often formed by disruption of the main pancreatic duct in the necrotized region. We radically treated intractable pancreatic juice fist...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200103000-00018
更新日期:2001-03-01 00:00:00
abstract::Several reports have described a loss of endogenous antioxidants and molecular oxidative damage during acute pancreatitis. Since hyaluronic acid and chondroitin-4-sulfate possess antioxidant properties, the effect of the administration of these glycosaminoglycans in a cerulein-induced acute pancreatitis in rats was in...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200403000-00019
更新日期:2004-03-01 00:00:00
abstract::250 syngeneic islets were implanted either beneath the kidney capsule or into the liver of diabetic LEW 1.A rats to investigate the functional response of a limited mass of beta-cells to long-term hyperglycemia. The number of islets, per se, was expected to be insufficient to reverse the hyperglycemia. All animals wer...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199403000-00008
更新日期:1994-03-01 00:00:00
abstract:OBJECTIVES:Ecological studies support the hypothesis that there is an association between vitamin D and pancreatic cancer (PaCa) mortality, but observational studies are somewhat conflicting. We sought to contribute further data to this issue by analyzing the differences in PaCa mortality across the eastern states of A...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31819975f4
更新日期:2009-05-01 00:00:00
abstract:OBJECTIVES:Clinical trials are a prerequisite for evidence-based medicine. Trial representativity by internal and external validities is an assumption for a transfer into the real world. METHODS:A questionnaire addressing several forms of bias was established. Randomized controlled trials for adjuvant treatment of pan...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31819d7370
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVES:During the past decade, numerous studies in both humans and animals have demonstrated that C-peptide, although not influencing blood sugar control, might play a role in preventing and potentially reversing some of the chronic complications of type 1 diabetes. The aim of this paper is to present an up-to-date...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/00006676-200410000-00009
更新日期:2004-10-01 00:00:00
abstract::Although ethanol abuse is the major etiologic factor in the development of acute and chronic pancreatitis, the mechanisms of ethanol effects to cause pancreatitis are poorly understood. The major reason for the lack of progress is the relative lack of animal models that reproduce the deleterious effects of ethanol on ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200311000-00004
更新日期:2003-11-01 00:00:00
abstract::Acute pancreatitis generates a complex cascade of immunological events that affect the pathogenesis and the progression of this disease. Several inflammatory mediators seem to play a critical role in the pathogenesis of pancreatitis and the subsequent inflammatory response. In turn, these mediators can influence hemos...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/01.mpa.0000240617.66215.d2
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Adhesion of tumor cells to extracellular matrix (ECM) proteins plays an important role in tumor invasion and metastasis. AIMS:To investigate the expression of integrins in human pancreatic cancer cell lines and its alteration by interleukin (IL)-1alpha to examine the mechanism of adhesion of metastatic hu...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200111000-00011
更新日期:2001-11-01 00:00:00
abstract:OBJECTIVES:Acute pancreatitis (AP) is a leading cause of hospitalization for a gastrointestinal illness in the United States. We hypothesized that enhanced recovery approaches may lead to earlier time to refeeding in patients with AP. METHODS:We performed a double-blind, randomized controlled trial of patients admitte...
journal_title:Pancreas
pub_type: 杂志文章,随机对照试验
doi:10.1097/MPA.0000000000001225
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVES:In patients with acute recurrent pancreatitis (ARP), pancreas divisum, and no other etiologic factors, endoscopic retrograde cholangiopancreatography (ERCP) with minor papilla endoscopic sphincterotomy (miES) is often performed to enlarge the minor papillary orifice, based on limited data. The aims of this s...
journal_title:Pancreas
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/MPA.0000000000001370
更新日期:2019-09-01 00:00:00
abstract::We diverted bile and pancreatic juice from jejunal blind loops of different lengths in conscious rats to see if the pancreatic secretory response was dependent on the length of the jejunal blind loop. Short-term bile and pancreatic juice diversion from a short jejunal blind loop, representing only 8-10% of the total l...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199901000-00007
更新日期:1999-01-01 00:00:00
abstract:INTRODUCTION:The consumption of green tea is associated with a lower risk of several types of human carcinomas. A number of studies have focused on the possible mechanisms of cancer prevention by tea extracts, especially polyphenols such as epigallocatechin-3-gallate (EGCG). AIMS AND METHODOLOGY:Green tea-derived EGCG...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200207000-00012
更新日期:2002-07-01 00:00:00
abstract:OBJECTIVE:This paper aimed to assess the physiological effects of p53 on glucose homeostasis in vivo. METHODS:A recombinant adenoviral p53 (rAd-p53) vector was administered to insulin-resistant diabetic mice. Intraperitoneal glucose tolerance test was performed in all groups of mice. Changes in fasting blood glucose, ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000637
更新日期:2016-08-01 00:00:00
abstract::The hedgehog (Hh) family of genes, sonic hedgehog (Shh), Indian hedgehog (Ihh), and desert hedgehog (Dhh) encode signaling molecules that regulate multiple functions during organ development and in adult tissues. Altered hedgehog signaling has been implicated in disturbed organ development as well as in different dege...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/01.mpa.0000202937.55460.0c
更新日期:2006-03-01 00:00:00
abstract:INTRODUCTION AND AIM:Recent studies have suggested that CCK is not essential for normal pancreatic growth in mice. We examined whether the treatment of hyperglycemia participates in a non-CCK-1-receptor-mediated mechanism of pancreatic regeneration after partial (30%) pancreatectomy (Px) with use of Otsuka Long-Evans T...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200305000-00010
更新日期:2003-05-01 00:00:00
abstract::This study compared diet, type of alcoholism, and smoking in three groups of alcoholic men, with chronic pancreatitis (n = 56), with histological cirrhosis (n = 50), and without pancreatitis or cirrhosis (controls; n = 50) by a multidimensional analysis. Only patients in whom the first symptom of pancreatitis or cirrh...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199504000-00003
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVES:This study aims to identify the computed tomography (CT) features that may differentiate nonfunctioning pancreatic neuroendocrine tumors (NF-PanNETs) with lymph node (LN) metastasis from NF-PanNETs without lymph node metastasis. METHODS:We retrospectively analyzed 166 NF-PanNETs in 166 patients who had unde...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000888
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:In patients with pancreaticobiliary maljunction (PBM), reflux of pancreatic juice to the bile duct may contribute to carcinogenesis of the biliary tract. This study aimed to investigate the pancreatographic findings in patients with PBM and the relationship to their clinical findings in view of pancreatic ju...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000153336.50482.59
更新日期:2005-04-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to establish a minimally invasive and simple screening test for detection of pancreatic ductal adenocarcinoma (PDAC) using duodenal juice (DJ). METHODS:Duodenal juice was collected prospectively before endoscopic retrograde cholangiopancreatography in 46 patients. A protease inhibi...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182649979
更新日期:2013-03-01 00:00:00
abstract::Because abnormalities of the autonomic nervous system have been described in several animal models of obesity, and because galanin has been proposed to be a sympathetic neurotransmitter in the endocrine pancreas, we hypothesized that the hyperinsulinemia observed in genetically obese (ob/ob) mice may result either fro...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199203000-00016
更新日期:1992-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of iodine contrast media and gadoteric acid in acute necrotizing pancreatitis. METHODS:Fifty rabbits were distributed in 5 groups: 10 rabbits were assigned in the control group (group 5) and 40 rabbits were assigned in the pancreatitis group, wherein acute necrotizing pancreatitis was...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/mpa.0b013e3180a6f366
更新日期:2007-11-01 00:00:00