当前位置： SCI文献检索 > JOURNAL OF NEUROIMMUNOLOGY期刊下所有文献 > 2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis.

2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis.

Abstract：

:2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide(89-104), total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.

journal_name

J Neuroimmunol

journal_title

Journal of neuroimmunology

authors

Farinazzo A,Gini B,Milli A,Ruffini F,Marconi S,Turano E,Anghileri E,Barbieri F,Cecconi D,Furlan R,Bonetti B

doi

10.1016/j.jneuroim.2010.10.004

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

63-7

issue

1-2

eissn

0165-5728

issn

1872-8421

pii

S0165-5728(10)00454-6

journal_volume

232

pub_type

杂志文章

相关文献

JOURNAL OF NEUROIMMUNOLOGY文献大全
  • Development of a cell-based assay for the detection of anti-aquaporin 1 antibodies in neuromyelitis optica spectrum disorders.

    abstract：OBJECTIVE:To develop a cell-based assay (CBA) to detect aquaporin 1 (AQP1) antibodies and determine sensitivity/specificity in patients with neuromyelitis optica (NMO) spectrum disorders. METHODS:A HEK-293T transfected cell model expressing AQP1 was established and detected to be serum AQP1 antibodies. RESULTS:AQP1 a...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2014.06.003

    authors： Long Y,Zheng Y,Shan F,Chen M,Fan Y,Zhang B,Gao C,Gao Q,Yang N

    更新日期：2014-08-15 00:00:00

  • Transforming growth factor-beta 1 inhibits tumor necrosis factor-alpha/lymphotoxin production and adoptive transfer of disease by effector cells of autoimmune encephalomyelitis.

    abstract：:We previously reported that the CD4+ suppressor cells (Ts) that regulate recovery of Lewis rats from experimental autoimmune encephalomyelitis (EAE) produce transforming growth factor-beta (TGF-beta). We also reported that TGF-beta downregulates interferon-gamma (IFN-gamma), but not interleukin-2 (IL-2) production, by...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(94)90131-7

    authors： Stevens DB,Gould KE,Swanborg RH

    更新日期：1994-04-01 00:00:00

  • Serial blood T cell repertoire alterations in multiple sclerosis patients; correlation with clinical and MRI parameters.

    abstract：:A significant skewing of the peripheral T cell repertoire has been shown in relapsing-remitting multiple sclerosis (MS). Most of the studies already performed in this field are cross-sectional and therefore, little is known of the T cell repertoire evolution over time in MS and the correlation of T cell repertoire var...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2006.05.006

    authors： Laplaud DA,Berthelot L,Miqueu P,Bourcier K,Moynard J,Oudinet Y,Guillet M,Ruiz C,Oden N,Brouard S,Guttmann CR,Weiner HL,Khoury SJ,Soulillou JP

    更新日期：2006-08-01 00:00:00

  • Obstacles to identifying viruses that cause autoimmune disease.

    abstract：:In addition to a clear genetic disposition, environmental factors and viral infections are thought to play a role in the pathogenesis of autoimmune diseases such as type I diabetes or multiple sclerosis (MS). This article will explore, by use of paradigms developed in a transgenic mouse model, why it has been so diffi...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0165-5728(00)00227-7

    authors： von Herrath MG

    更新日期：2000-07-24 00:00:00

  • Arecoline a muscarinic cholinergic agent conditions central pathways that modulate natural killer cell activity.

    abstract：:The central nervous system plays an active role in the regulation of the immune system. Modulation of immune activities appears to be in part under the control of the hypothalamic-pituitary-adrenal (HPA) axis. We investigated the effect of a muscarinic cholinergic agonist, arecoline, which stimulates the secretion of ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(95)00025-w

    authors： Demissie S,Rogers CF,Hiramoto NS,Ghanta VK,Hiramoto RN

    更新日期：1995-06-01 00:00:00

  • Tolerogenic forms of auto-antigens and cytokines in the induction of resistance to experimental allergic encephalomyelitis.

    abstract：:Resistance to experimental allergic encephalomyelitis (EAE) induction by homogenized myelin (MSCH) in complete Freund's adjuvant (CFA) and pertussigen (P) in SJL mice was seen 1 week after intravenous injection of PLP 139-151 coupled to spleen cells (PLP-ECDI-SP). Although this resistance could be transferred by splee...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(95)00022-t

    authors： Santambrogio L,Crisi GM,Leu J,Hochwald GM,Ryan T,Thorbecke GJ

    更新日期：1995-05-01 00:00:00

  • A potential link between autoimmunity and neurodegeneration in immune-mediated neurological disease.

    abstract：:Multiple sclerosis (MS) patients make antibodies to heterogeneous nuclear ribonuclear protein A1 (hnRNP-A1), a nucleocytoplasmic protein. We hypothesized this autoimmune reaction might contribute to neurodegeneration. Antibodies from MS patients reacted with hnRNP-A1-'M9', its nuclear translocation sequence. Transfect...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2011.02.007

    authors： Lee S,Xu L,Shin Y,Gardner L,Hartzes A,Dohan FC,Raine C,Homayouni R,Levin MC

    更新日期：2011-06-01 00:00:00

  • Intravenous immunoglobulin suppresses experimental myasthenia gravis: immunological mechanisms.

    abstract：:Intravenous immunoglobulin (IVIG) administration has been beneficially used in the treatment of several autoimmune disorders including myasthenia gravis (MG), although its mechanism of action is still not clear. To study the optimal conditions of IVIG treatment and delineate its mechanism of action we established a su...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2006.04.011

    authors： Zhu KY,Feferman T,Maiti PK,Souroujon MC,Fuchs S

    更新日期：2006-07-01 00:00:00

  • Serum CD95 of relapsing remitting multiple sclerosis patients protects from CD95-mediated apoptosis.

    abstract：:Failure of CD95-mediated apoptosis as a potential negative regulatory mechanism of T cell expansion may be involved in T cell-mediated autoimmune diseases such as multiple sclerosis (MS). Recently, soluble CD95 has been shown to be elevated in MS patients with active disease. Here, we report that the sera of MS patien...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00032-0

    authors： Zipp F,Otzelberger K,Dichgans J,Martin R,Weller M

    更新日期：1998-06-15 00:00:00

  • Amelioration of CR-EAE with lisofylline: effects on mRNA levels of IL-12 and IFN-gamma in the CNS.

    abstract：:CR-EAE is a Th1-mediated inflammatory autoimmune demyelinating disease of the CNS and serves as a model of human multiple sclerosis. Our previous studies have shown the protective effect of orally administered lisofylline in the prevention of active and passively induced acute EAE. In our present studies we have exami...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(00)00348-9

    authors： Du C,Cooper JC,Klaus SJ,Sriram S

    更新日期：2000-10-02 00:00:00

  • Cyclophosphamide alters the clinical and pathological expression of experimental autoimmune gray matter disease.

    abstract：:Guinea pigs inoculated with bovine spinal cord ventral horn homogenate develop a syndrome termed experimental autoimmune gray matter disease (EAGMD) characterized by extremity weakness, bulbar signs, and a loss of lower and upper motoneurons. To provide evidence for the role of autoimmune mechanisms, we have administe...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(91)90123-o

    authors： Tajti J,Stefani E,Appel SH

    更新日期：1991-11-01 00:00:00

  • Characterization of naïve, memory and effector T cells in progressive multiple sclerosis.

    abstract：:We characterized naïve, central memory (CM), effector memory (EM) and terminally differentiated effector memory (TEMRA) CD4+ and CD8+ T cells and their expression of CD49d and CD26 in peripheral blood in patients with multiple sclerosis (MS) and healthy controls. CD26+ CD28+ CD4+ TEMRA T cells were increased in all su...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2017.06.001

    authors： Nielsen BR,Ratzer R,Börnsen L,von Essen MR,Christensen JR,Sellebjerg F

    更新日期：2017-09-15 00:00:00

  • Antibody directed against mannan of the Mycobacterium tuberculosis cell envelope provokes blood-brain barrier breakdown.

    abstract：:Previous studies demonstrated that blood-brain barrier (BBB) breakdown observed at the cerebral level during experimental allergic encephalomyelitis (EAE) arises from the presence of Mycobacterium tuberculosis in Freund's adjuvant and not only from the encephalitogenic antigens. The main objective of this study was to...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(99)00236-2

    authors： Namer IJ,Steibel J

    更新日期：2000-02-01 00:00:00

  • Platelet-activating factor acetylhydrolase gene polymorphism and its activity in Japanese patients with multiple sclerosis.

    abstract：:We evaluated the association of the plasma platelet-activating factor acetylhydrolase (PAF-AH) gene polymorphism (G(994)-->T) and PAF-AH activity with susceptibility and severity of multiple sclerosis (MS) in Japanese. DNA was collected from 216 patients with clinically definite MS (65 opticospinal MS (OS-MS) and 151 ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2004.01.008

    authors： Osoegawa M,Niino M,Ochi H,Kikuchi S,Murai H,Fukazawa T,Minohara M,Tashiro K,Kira J

    更新日期：2004-05-01 00:00:00

  • Immunologic effects of cyclophosphamide/ACTH in patients with chronic progressive multiple sclerosis.

    abstract：:We examined immune function and changes in T cell populations over a 1-year period in a series of progressive multiple sclerosis (MS) patients treated with different regimens of cyclophosphamide/ACTH as part of the Northeast Multiple Sclerosis Treatment Group. Our studies were designed to determine the effect of diffe...

    journal_title：Journal of neuroimmunology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0165-5728(91)90007-t

    authors： Hafler DA,Orav J,Gertz R,Stazzone L,Weiner HL

    更新日期：1991-05-01 00:00:00

  • T-cell-mediated epineurial vasculitis and humoral-mediated microangiopathy in cryoglobulinemic neuropathy.

    abstract：:We used immunohistochemistry to assess the role of humoral and cellular factors in endoneurial microangiopathy and epineurial vasculitis in 15 nerve biopsies of patients with axonal neuropathy and monoclonal or mixed cryoglobulinemia (CG). Deposition of immunoglobulins and cytolytic complement was detected in endoneur...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(96)00191-9

    authors： Bonetti B,Invernizzi F,Rizzuto N,Bonazzi ML,Zanusso GL,Chinaglia G,Monaco S

    更新日期：1997-03-01 00:00:00

  • Upregulation of intercellular adhesion molecule-1 (ICAM-1) expression in primary cultures of human brain microvessel endothelial cells by cytokines and lipopolysaccharide.

    abstract：:The expression of intercellular adhesion molecule-1 (ICAM-1) by human cerebral endothelium was studied in primary cultures of human brain microvessel endothelial cells following treatment with bacterial lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interferon-gam...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(92)90170-p

    authors： Wong D,Dorovini-Zis K

    更新日期：1992-07-01 00:00:00

  • Impaired immune responsiveness is an essential component in persistent central nervous system infection with gross murine leukemia virus.

    abstract：:Exposure of newborn mice to Gross murine leukemia virus (GMuLV) results in persistent viral infection of the central nervous system (CNS) white matter. Animals exposed to virus as neonates showed a marked depression in GMuLV-specific B lymphocyte function as evidenced by significant decreases in adult and neonatal ant...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(91)90093-m

    authors： Korostoff JM,Nakada MT,Markman JF,Gaulton GN

    更新日期：1991-10-01 00:00:00

  • Extracellular matrix antigen of human muscle defined by a monoclonal antibody.

    abstract：:A monoclonal antibody (7.1B3) has been produced in mice previously immunised with a glycoprotein fraction prepared from human myotube cells grown in tissue culture. The antibody precipitates an antigen complex of four glycoprotein components from detergent extracted human muscle myotube cultures. These are two chains ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(83)90023-1

    authors： Walsh FS,Phillips E,Dhut S,Moore SE

    更新日期：1983-08-01 00:00:00

  • Citrullinated myelin basic protein induces experimental autoimmune encephalomyelitis in Lewis rats through a diverse T cell repertoire.

    abstract：:An increased proportion of citrullinated MBP (MBP-C8) occurs in the brains of multiple sclerosis (MS) patients. In this study, MBP-C8 from guinea pig (GP) brains was isolated and found encephalitogenic in Lewis rats upon immunization. An encephalitogenic T cell line selected with MBP-C8 preferentially reacted with MBP...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00063-0

    authors： Cao L,Sun D,Whitaker JN

    更新日期：1998-08-01 00:00:00

  • Immunocytochemical characterisation of cell cultures grown from dissociated 1-2-day post-natal rat cerebral tissue. A developmental study.

    abstract：:A range of cell-specific markers have been employed with immunocytochemical methods to characterise and quantitate the cell types present in mixed brain cell cultures derived from dissociated 1-2-day post-natal rat cerebral hemispheres and grown in the presence of FCS. Protoplasmic astrocytes (GFAP+, A2B5-) were the m...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(84)80002-8

    authors： Walker AG,Chapman J,Bruce CB,Rumsby MG

    更新日期：1984-11-01 00:00:00

  • Antibody neutralization of vascular endothelial growth factor (VEGF) fails to attenuate vascular permeability and brain edema in experimental pneumococcal meningitis.

    abstract：:To determine the contribution of vascular endothelial growth factor (VEGF) to cerebral edema formation in bacterial meningitis, we used a VEGF neutralizing antibody to block VEGF in rabbits, following induction of meningitis by intracisternal inoculation with 10(9) heat-killed pneumococci. At 8 h, cerebrospinal fluid ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2004.11.013

    authors： van der Flier M,Coenjaerts FE,Mwinzi PN,Rijkers E,Ruyken M,Scharringa J,Kimpen JL,Hoepelman AI,Geelen SP

    更新日期：2005-03-01 00:00:00

  • Detection and identification of virus-specific, oligoclonal IgG in unconcentrated cerebrospinal fluid by immunoblot technique.

    abstract：:A technique is described which allows the detection of virus-specific oligoclonal IgG in unconcentrated cerebrospinal fluid (CSF) from patients with virus infections of the central nervous system. CSF is isoelectrically focused in agarose gels and immunoglobulins are blotted to nitrocellulose filters, passively loaded...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(84)80008-9

    authors： Dörries R,Ter Meulen V

    更新日期：1984-12-01 00:00:00

  • No association of vitamin D-binding protein gene polymorphisms in Japanese patients with MS.

    abstract：:Vitamin D-binding protein (DBP) is known to function as an immunomodulatory factor, as well as the main carrier of vitamin D. We analyzed the frequencies of two polymorphisms (codon 416 and codon 420) in the DBP gene through a case-control study involving 107 Japanese patients with multiple sclerosis (MS) and 109 heal...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(02)00099-1

    authors： Niino M,Kikuchi S,Fukazawa T,Yabe I,Tashiro K

    更新日期：2002-06-01 00:00:00

  • Activated microglial cells acquire an immature dendritic cell phenotype and may terminate the immune response in an acute model of EAE.

    abstract：:Antigen presentation, a key mechanism in immune responses, involves two main signals: the first is provided by the engagement of a major histocompatibility complex (MHC), class I or class II, with their TCR receptor in lymphocytes, whereas the second demands the participation of different co-stimulatory molecules, suc...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2010.03.021

    authors： Almolda B,González B,Castellano B

    更新日期：2010-06-01 00:00:00

  • Type I interferon signals control Theiler's virus infection site, cellular infiltration and T cell stimulation in the CNS.

    abstract：:Theiler's murine encephalomyelitis virus (TMEV) establishes a persistent infection in the central nervous system (CNS). To examine the role of type I interferon (IFN-I)-mediated signals in TMEV infection, mice lacking a subunit of the type I IFN receptor (IFN-IR KO mice) were utilized. In contrast to wild type mice, I...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2010.05.028

    authors： Jin YH,Hou W,Kim SJ,Fuller AC,Kang B,Goings G,Miller SD,Kim BS

    更新日期：2010-09-14 00:00:00

  • Interferon-beta specifically inhibits interferon-gamma-induced class II major histocompatibility complex gene transcription in a human astrocytoma cell line.

    abstract：:We established cultures of human astrocytes and astrocytoma cells from surgical specimens, to study regulation of class II major histocompatibility (MHC) complex antigen expression by interferons. Using these cultures we previously showed that expression of the class II MHC determinant HLA-DR could be induced by inter...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(91)90054-b

    authors： Ransohoff RM,Devajyothi C,Estes ML,Babcock G,Rudick RA,Frohman EM,Barna BP

    更新日期：1991-08-01 00:00:00

  • Regulation of the cAMP cascade, gene expression and immune function by cannabinoid receptors.

    abstract：:The objective of this article is to discuss the putative role of cannabinoid receptors in immune modulation by cannabinoid compounds. The primary focus is on the signal transduction events that are initiated following ligand binding to cannabinoid receptors and how these events lead to detrimental effects on the norma...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0165-5728(97)00228-2

    authors： Kaminski NE

    更新日期：1998-03-15 00:00:00

  • Distinct macrophage subpopulations regulate viral encephalitis but not viral clearance in the CNS.

    abstract：:Intranasal application of vesicular stomatitis virus (VSV) induces acute encephalitis characterized by a pronounced myeloid and T cell infiltrate. The role of distinct phagocytic populations on VSV encephalitis was therefore examined in this study. Ablation of peripheral macrophages did not impair VSV encephalitis or ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2010.05.034

    authors： Steel CD,Kim WK,Sanford LD,Wellman LL,Burnett S,Van Rooijen N,Ciavarra RP

    更新日期：2010-09-14 00:00:00

  • Methylene blue alleviates experimental autoimmune encephalomyelitis by modulating AMPK/SIRT1 signaling pathway and Th17/Treg immune response.

    abstract：:Methylene blue (MB) is an effective neuroprotectant in many neurological disorders. AMP-activated protein kinase (AMPK)/silent mating-type information regulation 2 homolog 1 (SIRT1) plays a crucial role in maintaining inflammatory responses and shows a synergistic effect on cell homeostasis. We investigated the effect...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2016.08.014

    authors： Wang J,Zhao C,Kong P,Bian G,Sun Z,Sun Y,Guo L,Li B

    更新日期：2016-10-15 00:00:00