Abstract：
:A monoclonal antibody (7.1B3) has been produced in mice previously immunised with a glycoprotein fraction prepared from human myotube cells grown in tissue culture. The antibody precipitates an antigen complex of four glycoprotein components from detergent extracted human muscle myotube cultures. These are two chains of about 205K MW and two chains of about 130-140K MW. The antigen complex is trypsin-sensitive collagenase insensitive and bands as a complex of 10S on sucrose gradients in NP40. Indirect immunofluorescence studies on cell cultures of myoblasts and myotubes showed that the antigen existed in a number of locations: as a granular stain on the cell surface of myotubes, and as short streaks of immunoreactivity in the extracellular matrix. Clonal populations of myoblasts and myotubes gave a similar result showing that 7.1B3 is specifically synthesized by cells of the myogenic lineage. Double indirect immunofluorescence staining with 7.1B3 antibody and fibronectin antibody showed no overlap of immunoreactivity indicating that these two antigens are different. 7.1B3 antigen was found during human fetal muscle development in vivo at all time points studied from 15 weeks onwards. Immunoreactivity was localised to the loose extracellular matrix and in the endomysium. Perymysium staining was very prominent at 25 weeks and in adult muscle. Double staining of 7.1B3 antibody and alpha-bungarotoxin was carried out to determine whether 7.1B3 was present in synaptic junctions. Although 7.1B3 was found in the endomysium it is excluded from synaptic areas. We conclude that 7.1B3 antibody defines a new and novel antigen in the extracellular matrix of human muscle in vivo and in vitro.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Walsh FS,Phillips E,Dhut S,Moore SEdoi
10.1016/0165-5728(83)90023-1subject
Has Abstractpub_date
1983-08-01 00:00:00pages
11-31issue
1eissn
0165-5728issn
1872-8421pii
0165-5728(83)90023-1journal_volume
5pub_type
杂志文章abstract：:Tissue cysts, the hallmark of chronic Toxoplasma gondii infection, are predominantly located in the brain making clearance of the parasite difficult. Currently available anti-T. gondii drugs are ineffective on cysts and fail to prevent reactivation of latent toxoplasmosis. We examined whether abrogation of inhibitory ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
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更新日期：2018-06-15 00:00:00
abstract：:In order to evaluate changes in lymphocyte subpopulations of cerebrospinal fluid (CSF) cells in neurological diseases, normal control data have to be established. In this study we evaluated CSF samples from 65 dogs of both sexes and various breeds with an age range between 5 months and 6 years, and 20 one-year-old hea...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/S0165-5728(97)00192-6
更新日期：1998-02-01 00:00:00
abstract：:Gangliosides modulate various T cell effector functions through poorly defined mechanisms. To begin to understand one of their effects, the present study examined how normal brain-derived gangliosides suppress T cell proliferation using the murine T cell line, EL4, as a model. Gangliosides inhibited EL4 cell growth by...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00038-1
更新日期：1998-07-01 00:00:00
abstract：:An epistatic gene interaction has been advocated to explain disease susceptibility in multiple sclerosis (MS). Cytokine genes are possible candidates due to the central role played by cytokines in the regulation of the immune-mediated pathogenetic process leading to central nervous system demyelination in these patien...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00058-1
更新日期：1997-06-01 00:00:00
abstract：:Nitric oxide (NO) is implicated in inflammation and hypothalamic-pituitary responses to immune stimuli; however, the specific role of NO from neurons during stress-induced immune responses remains unspecified. We measured antigen-specific delayed-type-hypersensitivity (DTH) responses in the skin of wild-type (WT) and ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(03)00175-9
更新日期：2003-07-01 00:00:00
abstract：:Thymic tissues express transcripts encoding the alpha-3, alpha-5 and beta-4 subunits of nicotinic neuronal acetylcholine receptors (AcChRs) suggesting that neuronal AcChRs similar to those expressed in ganglia are expressed in the thymus. Transcription occurs in both isolated thymocytes and thymic epithelial cells. RT...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00120-3
更新日期：1997-11-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2012.08.013
更新日期：2013-01-15 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.07.017
更新日期：2014-09-15 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.06.008
更新日期：2006-10-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-5728(99)00197-6
更新日期：1999-12-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(83)90012-7
更新日期：1983-04-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(99)00229-5
更新日期：2000-03-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2020.577292
更新日期：2020-06-16 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(00)00445-8
更新日期：2001-03-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(94)90218-6
更新日期：1994-02-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2005.09.012
更新日期：2006-02-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(92)90154-d
更新日期：1992-03-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
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更新日期：1996-02-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(92)90176-l
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
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更新日期：1983-04-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(94)90032-9
更新日期：1994-09-01 00:00:00
abstract：:Fas (CD95) and Fas ligand (FasL, CD95L) have been implicated to be involved in the acute inflammatory response by attracting neutrophils and regulating their survival. Increased levels of soluble Fas and FasL are found in cerebrospinal fluid (CSF) samples of patients with bacterial meningitis but not in controls. Func...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.04.004
更新日期：2004-07-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(02)00023-1
更新日期：2002-04-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(87)90065-8
更新日期：1987-01-01 00:00:00
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journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/j.jneuroim.2009.09.013
更新日期：2010-03-30 00:00:00
abstract：:We investigated T cell immunity to Epstein-Barr virus (EBV) in multiple sclerosis (MS) and assessed cross-reactivity of the anti-EBV cellular response with brain antigens. We measured the proliferation, cytotoxicity, and number of interferon-γ secreting cells following stimulation with autologous EBV-infected lymphobl...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2010.08.009
更新日期：2010-12-15 00:00:00
abstract：:Psychological and physical stress can alter the immune system in both humans and animals. We have reported that mice subjected to chronic 12-h daily physical restraint for 2 days showed dramatic apoptosis in splenocytes. To identify genes that contribute to the splenocyte apoptosis, we compare gene expression in the s...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.05.004
更新日期：2006-08-01 00:00:00
abstract：:Microglial activation is the most common phenomenon in S. aureus induced brain abscesses as well as other common neurodegenerative diseases. The main objective of this study is to reduce the microglial inflammation with effective bacterial elimination. Ciprofloxacin and celecoxib were used in combination to regulate S...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.12.006
更新日期：2018-03-15 00:00:00
abstract：:Microglial inflammation plays a pivotal role in the pathogenesis of S. aureus induced brain abscesses. The objective of this study was to regulate microglial activation by the combinatorial treatment of ciprofloxacin either with dexamethasone or celecoxib via targeting M1 and M2 polarization. The antibiotic-immunomodu...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
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更新日期：2020-07-15 00:00:00
