Abstract:
:Receptors for VIP in mouse peritoneal macrophages (MPM) were examined using [125I]labeled VIP as ligand. The receptor binding was rapid, reversible, saturable, specific, and dependent on time, pH, temperature and cell concentration. At 15 degrees C, the stoichiometric data suggested the presence of two classes of VIP receptors with Kd values of 1.05 +/- 0.2 and 66.4 +/- 11.0 nM and binding capacities of 19.2 +/- 2.8 and 706.6 +/- 172.0 fmol VIP/10(6) cells. The interaction showed a high degree of specificity, as suggested by competition experiments with various peptides structurally related to VIP as follows: VIP > helodermin > rGRF > PHI > secretin. Glucagon, pancreastatin, somatostatin, insulin, and octapeptide of cholecystokinin (CCK 26-33) were ineffective at concentrations as high as 1 microM. VIP was a potent and efficient stimulator of cyclic AMP production in MPM. The stimulation was observed at a concentration as low as 0.01 nM VIP. Half-maximal stimulation (ED50) was observed at 1.0 +/- 0.2 nM VIP, and maximal stimulation (three-fold above basal levels) was obtained between 0.1-1 microM. The cyclic AMP system of mouse peritoneal macrophages showed a high specificity for VIP. The order of potency observed in inducing cyclic AMP production was VIP > helodermin > rGRF > PHI > secretin. Glucagon, insulin, pancreastatin, somatostatin and octapeptide of cholecystokinin did not modify cyclic AMP levels at concentrations as high as 1 microM.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Calvo JR,Montilla ML,Guerrero JM,Segura JJdoi
10.1016/0165-5728(94)90218-6subject
Has Abstractpub_date
1994-02-01 00:00:00pages
85-93issue
1eissn
0165-5728issn
1872-8421pii
0165-5728(94)90218-6journal_volume
50pub_type
杂志文章abstract::Intercellular adhesion molecule-1 (ICAM-1) is a cell surface glycoprotein which can be induced on astrocytes, the major glial cell of the central nervous system (CNS). In this study, we examined the effect of three proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)90083-3
更新日期:1994-05-01 00:00:00
abstract::Transmitters and cotransmitters of the sympathetic nervous system are involved in the regulation of a variety of immune cell functions. However, it is not entirely clear what stimuli lead to the release of these molecules in immune organs. In this study, we investigated whether local ischemia can cause the parallel re...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(00)00365-9
更新日期:2000-11-01 00:00:00
abstract::HLA-DR, Iba1 and CD68 are widely used microglia markers in human tissue. However, due to differences in gene regulation, they may identify different activation stages of microglia. Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in microglia with different phenotypes, ranging from ramified to amoebo...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2017.04.007
更新日期:2017-08-15 00:00:00
abstract:OBJECTIVE:It's hypothesized that gastrointestinal microbiota might play an important role in pathogenesis of multiple sclerosis (MS). The aim of the present study was to assess the prevalence of small intestinal bacterial overgrowth (SIBO) in MS patients compared with sex and age matched controls without MS. METHODS:T...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2016.11.004
更新日期:2016-12-15 00:00:00
abstract::Immunological events occurring in the central nervous system (CNS) as a result of head trauma are largely unexplored. We report here that the levels of the alternative pathway complement proteins C3 and factor B are elevated in the cerebrospinal fluid (CSF) of head-injured patients. C3 and factor B indices suggest tha...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(96)00164-6
更新日期:1997-03-01 00:00:00
abstract::Neuronal apoptosis has recently been implicated in multiple sclerosis (MS). Apoptotic cell death of neurons is induced in cultures exposed to cerebrospinal fluid (CSF) from MS patients. Since caspases are essential in the regulation of apoptosis, direct evidence was sought linking caspases to CSF-induced neuronal deat...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(02)00467-8
更新日期:2003-03-01 00:00:00
abstract::Human G-protein-coupled formyl peptide receptor-like 1 and its mouse homologue formyl peptide receptor 2 mediate the chemotactic activity of a variety of pathogen and host-derived peptides, including amyloid beta(42), a key causative factor in Alzheimer's disease (AD).Here, we found that polyinosine-polycytidylic acid...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2009.05.018
更新日期:2009-08-18 00:00:00
abstract::Opsoclonus is an ocular motility disorder characterized by spontaneous, arrhythmic conjugate saccades of varying amplitude occurring in all directions of gaze without normal intersaccadic interval. Etiological spectrum of opsoclonus encompasses paraneoplastic and neoplastic conditions, infectious and para-infectious e...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2020.577394
更新日期:2020-11-15 00:00:00
abstract::Mitoxantrone, a new antineoplastic anthracenedione drug, suppressed the development of experimental allergic encephalomyelitis (EAE) in rats when administered during the incubation period. One dose of mitoxantrone had a favorable effect on EAE even when withheld until after the onset of clinical signs. In a model of r...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(86)90063-9
更新日期:1986-12-01 00:00:00
abstract::Brain capillary endothelial cells are the component of blood brain barrier, and the first line of defense against viruses invading into brain. We demonstrate that treatment of hCMEC/D3 cells, a human brain capillary endothelial cell line, with a Toll-like receptor 3 (TLR3) agonist polyinosinic-polycytidylic acid (poly...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2018.08.016
更新日期:2018-11-15 00:00:00
abstract::We established cultures of human astrocytes and astrocytoma cells from surgical specimens, to study regulation of class II major histocompatibility (MHC) complex antigen expression by interferons. Using these cultures we previously showed that expression of the class II MHC determinant HLA-DR could be induced by inter...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(91)90054-b
更新日期:1991-08-01 00:00:00
abstract::Interactions between PD-1 and its two differentially expressed ligands, PD-L1 and PD-L2, attenuate T cell activation and effector function. To determine the role of these molecules in autoimmune disease of the CNS, PD-1-/-, PD-L1-/- and PD-L2-/- mice were generated and immunized to induce experimental autoimmune encep...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2006.10.006
更新日期:2007-01-01 00:00:00
abstract::Rat thymocytes incubated in RPMI medium for 120-300 min release a soluble factor of molecular weight below 10,000 Da which inhibits the binding of 3H-labeled muscarinic antagonists in an uncompetitive manner, i.e. it reduces maximal specific binding (Bmax) without changing the affinity of the ligand (KD). This factor ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(88)90119-1
更新日期:1988-03-01 00:00:00
abstract::Patients with progressive multiple sclerosis (MS) and controls were compared with regard to: (a) in vitro pokeweed mitogen (pwm)-induced IgG secretion, as an indirect measure of T8+ cell-mediated suppressor function; (b) alloantigen-directed cytotoxic activity, a predominantly T8+ cell-mediated function. The MS group ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(86)80005-4
更新日期:1986-09-01 00:00:00
abstract::Opioid-containing immunocytes migrate to inflamed sites where they release beta-endorphin which activates peripheral opioid receptors and produces analgesia. The immigration of immunocytes to sites of inflammation is mediated by adhesion molecules. In this study, the expression of L-, P-, E-selectin and platelet-endot...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(00)00284-8
更新日期:2000-08-01 00:00:00
abstract::Migraine is believed to be a chronic neurological disorder with the exact aetiology being unknown. But, there is a debate on the role of immune dysfunction in migraine pathophysiology. Hence, authors made a debut attempt to explore the link between lymphocyte subset populations and migraine. A significant increase in ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2015.11.015
更新日期:2016-01-15 00:00:00
abstract::The aim of the current review is to summarize the results of studies on the role of γδ T cells in the pathogenesis of multiple sclerosis and its animal model - the experimental autoimmune encephalomyelitis. Despite the fact that numerous studies have been performed, the role of γδ T is still not fully understood. It s...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章,评审
doi:10.1016/j.jneuroim.2019.02.009
更新日期:2019-05-15 00:00:00
abstract::Human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory neurological disease caused by HTLV-I infection. It has been shown that HAM/TSP patients have high proviral loads and an extraordinarily high frequency of circulating CD8 + cytotoxic T lymphocytes...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00175-7
更新日期:2000-01-24 00:00:00
abstract::Differences in the regulation of surface molecule expression and functional activity of microglia, the resident immune effector elements of the central nervous system (CNS), might give important insights into understanding the predilection sites of some diseases within the CNS. Therefore, canine microglial cells in re...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.07.017
更新日期:2010-10-08 00:00:00
abstract::We investigated whether microglia form gap junctions with themselves, or with astrocytes, oligodendrocytes, or neurons in vivo in normal mouse brains, and in pathological conditions that induce microglial activation - brain injury and a model of Alzheimer's disease. Although microglia are in close physical proximity t...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2014.02.005
更新日期:2014-04-15 00:00:00
abstract::The multifaceted pathogenesis of multiple sclerosis (MS) involves complex interactions between the immune and the central nervous systems which provide a multitude of targets for therapeutic interventions. Current therapies for MS are only partially effective. One potential strategy to increase treatment efficacy is t...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章,评审
doi:10.1016/j.jneuroim.2010.10.021
更新日期:2011-02-01 00:00:00
abstract::To explore the hypothesis that gammadelta T cells may regulate activated alphabeta T cells, we studied gammadelta T cell responses to alphabeta T cell clones in Multiple Sclerosis (MS) patients who received attenuated autologous autoreactive T cells. We recently conducted a pilot study of T cell vaccination with myeli...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00060-5
更新日期:1998-07-01 00:00:00
abstract::The extent to which symptomatic improvement in patients with multiple sclerosis treated with intravenous methylprednisolone depends on immunological actions of corticosteroids is unknown. In this study the effect of methylprednisolone on circulating T and B cell function has been assessed in vitro and in vivo. Low mol...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(90)90114-3
更新日期:1990-01-01 00:00:00
abstract::In this report, we show that the human astroglioma cell line, D54-MG, constitutively expresses C3 mRNA and secretes antigenically detectable C3 protein. The cytokine interferon-gamma (IFN-gamma) enhances C3 mRNA and protein expression by D54-MG cells in a dose- and time-dependent manner. C3 mRNA from both D54-MG cells...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(92)90020-l
更新日期:1992-06-01 00:00:00
abstract::Degeneration of cholinergic neurons in the medial septum and the diagonal band of Broca is a frequent neuropathological feature of Alzheimer's disease. To determine whether an immune process can injure these basal forebrain cholinergic neurons, we serially immunized guinea pigs with septal cholinergic hybrid cells (SN...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00062-3
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Autoreactive antibodies (ARAB) occur more frequently in patients with multiple sclerosis (MS) than in general population and the presence of these antibodies often causes uncertainty regarding the disease course, response to therapy and the diagnosis of MS. METHODS:Retrospective analyses of the ARAB, clinic...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.04.008
更新日期:2007-07-01 00:00:00
abstract::The effects of acute or chronic in vivo or in vitro exposures to supra-physiologic doses of isoproterenol, insulin or dexamethasone on rat splenic lymphocyte proliferation were investigated. Acutely, all in vivo challenges inhibited mitogen-induced lymphocyte proliferation, and correlated to increased corticosterone l...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(87)90079-8
更新日期:1987-10-01 00:00:00
abstract::Experimental autoimmune myasthenia gravis (EAMG) was induced in rats by injection of purified acetylcholine receptor (AChR). In addition to detecting elevated serum titers of anti-AChR antibodies, we observed decreased twitch-tension at submaximal stimulation voltages and increased curare sensitivity by muscles obtain...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(87)90013-0
更新日期:1987-04-01 00:00:00
abstract::Immunotherapeutic approaches to treating Alzheimer's disease (AD) using vaccination strategies must overcome the obstacle of achieving adequate responses to vaccination in the elderly. Here we demonstrate for the first time that application of the Escherichia coli heat-labile enterotoxin adjuvant-laden immunostimulato...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2014.01.002
更新日期:2014-03-15 00:00:00
abstract::Sprague-Dawley male rats implanted with chronic indwelling cannulae at the perifornical hypothalamus eat excessively during the sixth hour following administration of exogenous immune-complexing reactants to the brain site. Rabbit anti-HSA was injected, followed in 30 min by a 20-fold excess of antigen. Anaphylatoxin ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(87)90065-8
更新日期:1987-01-01 00:00:00